Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,797,083 C→T V288M (GTG→ATG)  pheT ← phenylalanine‑‑tRNA ligase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,797,0830CT98.0% 174.3 / ‑4.0 51V288M (GTG→ATG) pheTphenylalanine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (26/24);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ACCGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGT  >  NC_000913/1796790‑1797378
                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                       
accGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATANCGGTTAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGt                                                                                                                                                                                                                                                                                                   <  1:528242/300‑1 (MQ=255)
accGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATANCGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGt                                                                                                                                                                                                                                                                                                   <  1:528226/300‑1 (MQ=255)
accGCAGATGGCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTTAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCTAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCg                                                                                                                                                                                                                                                                                                    <  2:282565/299‑1 (MQ=255)
 ccGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCATTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGtt                                                                                                                                                                                                                                                                                                  >  1:215605/1‑300 (MQ=255)
       aTGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGccc                                                                                                                                                                                                                                                                                            <  1:567727/300‑1 (MQ=255)
                agcagACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGc                                                                                                                                                                                                                                                                                    <  1:516984/299‑1 (MQ=255)
                      cGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTNTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGATTCAGTACCGTCGAGCAGCATCAACGTTTCGCCCTCTTTCGCCATCCGc                                                                                                                                                                                                                                                                             >  2:589743/1‑300 (MQ=255)
                            gCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGcaccac                                                                                                                                                                                                                                                                        <  1:312031/299‑1 (MQ=255)
                                gTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACNATGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATgc                                                                                                                                                                                                                                                                   <  1:157210/300‑1 (MQ=255)
                                gTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATgc                                                                                                                                                                                                                                                                   <  1:135454/300‑1 (MQ=255)
                                  tCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTTAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATgccg                                                                                                                                                                                                                                                                 <  1:110124/300‑1 (MQ=255)
                                     aTCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCt                                                                                                                                                                                                                                                              <  2:689190/300‑1 (MQ=255)
                                        gCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCGGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCa                                                                                                                                                                                                                                                           >  1:433121/1‑300 (MQ=255)
                                        gCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCa                                                                                                                                                                                                                                                           >  1:21489/1‑300 (MQ=255)
                                                                      cGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGc                                                                                                                                                                                                                             >  1:454935/1‑300 (MQ=255)
                                                                       gCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCt                                                                                                                                                                                                                            >  1:688684/1‑300 (MQ=255)
                                                                          cATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATtggctggc                                                                                                                                                                                                                         >  2:752130/1‑300 (MQ=255)
                                                                                      aCGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGagca                                                                                                                                                                                                             >  1:668010/1‑300 (MQ=255)
                                                                                              gTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATa                                                                                                                                                                                                      >  1:642040/1‑299 (MQ=255)
                                                                                               tATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGt                                                                                                                                                                                                    <  1:214569/300‑1 (MQ=255)
                                                                                                      gcgagatgtgtataagagacaggTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGAc                                                                                                                                                                                             >  1:746052/22‑300 (MQ=255)
                                                                                                       ccATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGAc                                                                                                                                                                                             >  1:388915/1‑299 (MQ=255)
                                                                                                                            gTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACCACTGCATCGAt                                                                                                                                                                           >  1:197020/1‑296 (MQ=255)
                                                                                                                            gTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGaa                                                                                                                                                                       >  2:454196/1‑300 (MQ=255)
                                                                                                                             tGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTTGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAAc                                                                                                                                                                      <  2:452261/300‑1 (MQ=255)
                                                                                                                                        gggCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCa                                                                                                                                                            <  1:708523/299‑1 (MQ=255)
                                                                                                                                                                                    aTTCACGCCAGAGTGTTCGCCAACGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAAc                                                                                                               >  1:99301/1‑300 (MQ=255)
                                                                                                                                                                                                                 tGCCGCCCATCGCCAGCGCCTTGTTGTGGTCTGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCTTCGAGTAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGAt                                                                                  <  2:180985/300‑1 (MQ=255)
                                                                                                                                                                                                                 tGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGAt                                                                                  <  1:668507/300‑1 (MQ=255)
                                                                                                                                                                                                                 gcgagatgtgtataagagacaggttgtGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGAt                                                                                  >  1:679877/23‑300 (MQ=255)
                                                                                                                                                                                                                  gccgccCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATa                                                                                 <  1:454196/300‑1 (MQ=255)
                                                                                                                                                                                                                         aTCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAAcgc                                                                             >  1:664043/1‑297 (MQ=255)
                                                                                                                                                                                                                          tCGCCAGCGCCTTGTGGTGGTCTGCGAGGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGTTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCTGAGTTGGCGCTTTAACGTTAATGCCTTTTACCacac                                                                                           <  2:388915/282‑1 (MQ=255)
                                                                                                                                                                                                                                  gcCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACAGATCCCGCAACAACGCAGTTTNTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTcc                                                                 >  1:540834/1‑300 (MQ=255)
                                                                                                                                                                                                                                  gcCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTAGCTTAAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACCGCGGAGTTGGCTCTCTAACGTTCATGCCtttt                                                                                                  >  2:93707/1‑267 (MQ=255)
                                                                                                                                                                                                                                     ttgttgTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCTCCCNCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTTCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGTTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCGCACGGCCAAGATAACGCGGGCAGGCTTCCGgc                                                              <  1:548116/300‑1 (MQ=255)
                                                                                                                                                                                                                                     ttgttgTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAACGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGgc                                                              >  1:62111/1‑300 (MQ=255)
                                                                                                                                                                                                                                       gttgtGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCTAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGgcgc                                                            >  1:450985/1‑300 (MQ=255)
                                                                                                                                                                                                                                             gTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGAc                                                      <  2:432064/300‑1 (MQ=255)
                                                                                                                                                                                                                                                 gCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTa                                                  >  2:739606/1‑300 (MQ=255)
                                                                                                                                                                                                                                                 gCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTCTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCAGCAACGAAGCAGTTTTTCTTTAATCCACAGCGGAGTTGGCGCTTTAACGTTAATGTCTTTTACCACACGGCAAAGGTCAAGCGGGCAGGCTTCCGgcgc                                                            >  2:92693/1‑290 (MQ=255)
                                                                                                                                                                                                                                                     tGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGTTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCg                                              <  2:442292/300‑1 (MQ=255)
                                                                                                                                                                                                                                                       aCCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGc                                            <  2:454935/300‑1 (MQ=255)
                                                                                                                                                                                                                                                             gtgtCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCgt                                       >  2:428916/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                     aTTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCTGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGAt                              <  2:353615/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                             tcgcttcAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCAc                      <  2:502135/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                cttcAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGACAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCAccca                   >  2:59452/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                                cttcAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCaa                   >  1:704220/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                cttcAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGAACCa                    >  2:643347/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                cttcAGTACCGTCGAGCAGCATCAGCGTTTCGCACTATTTAGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACACCTGCATCGATAGAATGGATCCCGCAACGCCGCAGTCTTTCTTTCATCCACAGCGGAGTTGGCGCTTTCACGTTCATGCCTCTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTAGACTGTAATCGGCAGCGTGTCGTCGATGCTCGCACCaa                   >  2:275540/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                 agcaTCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTTACGTCAACAACGGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTCGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCTGAACGATTTCCGGt  <  2:89672/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                       
ACCGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGT  >  NC_000913/1796790‑1797378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑