Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,017,589	A→C	I346L (ATC→CTC)	fliI →	flagellar export ATPase FliI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,017,589	0	A	C	100.0%	157.7 / NA	44	I346L (ATC→CTC)	fliI	flagellar export ATPase FliI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/26);  total (18/26)

GGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAAC  >  NC_000913/2017297‑2017868                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                        gggTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTTAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGa                                                                                                                                                                                                                                                                                  >  1:165526/1‑300 (MQ=255) gggTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGa                                                                                                                                                                                                                                                                                  >  1:739180/1‑300 (MQ=255)  ggTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAACGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGAc                                                                                                                                                                                                                                                                                 <  2:653075/300‑1 (MQ=255)        gccTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGACCCGATTGCCGACTCCGCGCGGGCGCTCCt                                                                                                                                                                                                                                                                                     >  2:264690/1‑290 (MQ=255)        gccTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCAGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCTCGCGGGCGCTCCTCGACGGTcac                                                                                                                                                                                                                                                                           >  2:633146/1‑300 (MQ=255)             tGCCACGCGCAGTGCCGAAGGGTTTCGCGATCGTGTTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGAGTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATtg                                                                                                                                                                                                                                                                       <  2:215485/299‑1 (MQ=255)                 aCGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGc                                                                                                                                                                                                                                                                    <  1:360685/298‑1 (MQ=255)                 aCGCGCATGGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGc                                                                                                                                                                                                                                                                    <  1:372136/298‑1 (MQ=255)                   gcgcATTGCCGAATATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCANTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCNGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCGGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGc                                                                                                                                                                                                                                                                    <  2:570673/296‑1 (MQ=255)                     gcATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGtct                                                                                                                                                                                                                                                               <  1:313170/299‑1 (MQ=255)                     gcATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGGGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACNGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGtct                                                                                                                                                                                                                                                               <  2:237634/299‑1 (MQ=255)                                                             gCTGATGATGGACTCCTTCACCCTCTACGCGATTGCCCACCGTGAAATGGCGCTGGCAATTTGCGAACCCCCCGCCACCAAAGGCTATCCACNGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCATGCCGGAAATGGCATTAGCGGCGGCGGCTTGATTACCGCGTTTTGTACCGTGCTCACTGAGGGCGATGACCAGCAGGATCCGATTGCCGATTCCGCGCGGGCGctcctctgcgttcacactgttctttcc                                                                                                                                                                                                                                                               <  2:238280/259‑22 (MQ=255)                                                                 aTTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGaa                                                                                                                                                                                                                  >  1:291770/1‑300 (MQ=255)                                                                 aTTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCAAATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGaa                                                                                                                                                                                                                  >  1:291547/1‑300 (MQ=255)                                                                           cccTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCAAATTGTGCTGTCTCGCCGACTGGCGGAAGCAGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCa                                                                                                                                                                                                        >  2:375853/1‑300 (MQ=255)                                                                                                   aGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATc                                                                                                                                                                                 <  1:375853/299‑1 (MQ=255)                                                                                                    gCGTGAAATTGCGTTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATc                                                                                                                                                                                 <  2:189906/298‑1 (MQ=255)                                                                                                    gCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGTCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCGCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACGATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAg                                                                                                                                                                               <  2:488133/300‑1 (MQ=255)                                                                                                            ttGCGCTGGCGATTGGCGAACCCCCCGCCACCAAATGTTATCCACCATCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAAc                                                                                                                                                                       <  2:476137/300‑1 (MQ=255)                                                                                                                                      tccacaaAAGGGTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGTTCGAGCGTGCCGGAGATGGCATTAGCGTCGGCTGCTCGATTACCGCGTTTTATACCGTACTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCTTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACGTTACGCGCGAGTGCGCACCtc                                                                                                                                                 <  2:649261/295‑2 (MQ=255)                                                                                                                                      gCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCtt                                                                                                                                                 <  2:629040/296‑1 (MQ=255)                                                                                                                                      gCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGAT‑AGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAAc                                                                                                                                            <  1:608456/300‑1 (MQ=255)                                                                                                                                       agacggaaGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAAc                                                                                                                                            <  2:515800/294‑1 (MQ=255)                                                                                                                                         accaAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAAc                                                                                                                                            <  2:291770/298‑1 (MQ=255)                                                                                                                                         accaAAGGTTATCCACCGTCGGTGTTTGCCAAAGTACCGGCACTAGGCGAGCGTGCCGGAAGTGGCATTAGCGGCGGCGGCTCGATTACCGTGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAACCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAAc                                                                                                                                            <  2:291547/298‑1 (MQ=255)                                                                                                                                             aaGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAAc                                                                                                                                            >  1:515800/1‑294 (MQ=255)                                                                                                                                              aGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGCTTGCCGACTCCTCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCAGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCCGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCtgtt                                                                                                                                     >  1:309250/1‑300 (MQ=255)                                                                                                                                              aGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCTCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCtgtt                                                                                                                                     >  1:309657/1‑300 (MQ=255)                                                                                                                                              aGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCtgtt                                                                                                                                     >  1:43893/1‑300 (MQ=255)                                                                                                                                                         gtcagttgttgctcAAATTACCGGCACCGGTCGAGCGTGCCGGAAATGGCATTAGCTGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTATGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAAc                                                                                                                                            <  2:248732/269‑1 (MQ=255)                                                                                                                                                                   tGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACcg                                                                                                                <  2:388153/300‑1 (MQ=255)                                                                                                                                                                        aaTTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCGTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGagc                                                                                                           <  1:718080/300‑3 (MQ=255)                                                                                                                                                                                  aCTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGt                                                                                                 <  1:367013/300‑1 (MQ=255)                                                                                                                                                                                                                            gATTACCGCGTTTTATACCGTGCTCACTGAATGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGc                                                       <  1:671158/300‑1 (MQ=255)                                                                                                                                                                                                                            gATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGc                                                       <  1:220943/300‑1 (MQ=255)                                                                                                                                                                                                                            gATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATACGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATAGTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCGAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGTCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGc                                                       <  2:423087/300‑1 (MQ=255)                                                                                                                                                                                                                                   gcgTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGc                                                 <  1:496824/299‑1 (MQ=255)                                                                                                                                                                                                                                     gTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGNTCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCGTCGcact                                              >  2:218518/1‑297 (MQ=255)                                                                                                                                                                                                                                     gTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCt                                              >  1:417662/1‑300 (MQ=255)                                                                                                                                                                                                                                     gTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTAGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGACCCGATGCTCGATAAAGCCATCGCCCt                                              >  2:648941/1‑300 (MQ=255)                                                                                                                                                                                                                                     gTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCAGACTGGCGGAAGCCGGGCACTCTCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTATCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCt                                              >  1:705622/1‑300 (MQ=255)                                                                                                                                                                                                                                               gTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGACGCCTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCATATGCCAAAGGCAGCGATCCGATGTTCGATAAAGCCATCGCCCTGTGGCCGCa                                     >  2:374533/1‑299 (MQ=255)                                                                                                                                                                                                                                                    cACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGa                               >  1:574930/1‑300 (MQ=255)                                                                                                                                                                                                                                                        gAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGc                           <  1:567080/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  tGCCGACTCCGCGCGGGCGCTCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAAc  >  2:694938/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                        GGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCGATGACCAGCAGGATCCGATTGCCGACTCCGCGCGGGCGATCCTCGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGCCGCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAAC  >  NC_000913/2017297‑2017868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑