Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,556,984	G→A	T105I (ACC→ATC)	eutC ←	ethanolamine ammonia‑lyase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,556,984	0	G	A	100.0%	206.6 / NA	58	T105I (ACC→ATC)	eutC	ethanolamine ammonia‑lyase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/34);  total (24/34)

GAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGT  >  NC_000913/2556698‑2557268                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                             gAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCCCGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGAACTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAAc                                                                                                                                                                                                                                                                                  >  2:753831/1‑299 (MQ=255)      gtgtACCGACTTTCAGCCCTTCCTGTTTCGTGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCTGCACTTCTTTCAGTACGATATCTTTc                                                                                                                                                                                                                                                                                      <  2:720883/290‑1 (MQ=255)       tggaCCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCGGGATCTCTTCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGt                                                                                                                                                                                                                                                                            <  2:339292/296‑1 (MQ=255)               cTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCa                                                                                                                                                                                                                                                                   >  1:455383/1‑300 (MQ=255)                                   aGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCAT‑AGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCg                                                                                                                                                                                                                                              <  1:74195/300‑1 (MQ=255)                                    ggCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACTTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCTGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCg                                                                                                                                                                                                                                              <  1:114024/300‑1 (MQ=255)                                    ggCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCg                                                                                                                                                                                                                                              <  1:113634/300‑1 (MQ=255)                                         gCCATCAGCGGTGGCANGATCTCTTCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGANTGATCGGCCAGGAAACGCNGCAGCGCCTGCGTACGt                                                                                                                                                                                                                                         >  1:626681/1‑300 (MQ=255)                                            aTCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTg                                                                                                                                                                                                                                        <  2:715720/298‑1 (MQ=255)                                                        aGGATCTCTTCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCa                                                                                                                                                                                                                          <  1:707228/300‑1 (MQ=255)                                                                       ttCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACATGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAAcg                                                                                                                                                                                                           <  2:477293/300‑1 (MQ=255)                                                                       ttCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAAcg                                                                                                                                                                                                           <  1:402955/300‑1 (MQ=255)                                                                       ttCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTATACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATGTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAAcg                                                                                                                                                                                                           <  1:402771/300‑1 (MQ=255)                                                                        tCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAAcg                                                                                                                                                                                                           <  2:194888/299‑1 (MQ=255)                                                                            ggTGATCGCATCAGTGGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCANCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGc                                                                                                                                                                                                      <  2:527649/300‑1 (MQ=255)                                                                               gATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCAc                                                                                                                                                                                                   >  1:664961/1‑300 (MQ=255)                                                                                 tCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCAc                                                                                                                                                                                                   >  2:470506/1‑298 (MQ=255)                                                                                     aTCAGTTAACATGCCATCAGAAATAACGACCTGTACGTCCGTATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCTGGGCGAGTCAGGTAGAAATGGTTGTCGCTAATTTCAGAGCGTACGTCCAGCAAGCCCTGCGCTTTCACCCACTCGTCCGGCACTTCTTTCAGGACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGtgc                                                                                                                                                                                              <  2:290351/299‑1 (MQ=255)                                                                                         gTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACATTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGACCGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGAACGGCACGAACGGTACAAACGCGGGCCACGGtgct                                                                                                                                                                                             >  2:41648/1‑296 (MQ=255)                                                                                         gTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTgcgg                                                                                                                                                                                         <  1:107890/300‑1 (MQ=255)                                                                                               agaccATCAGAAATAACGACTTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTACACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCATCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTGCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTgcgg                                                                                                                                                                                         <  2:179985/291‑1 (MQ=255)                                                                                                 gCCATCAGAAATAACGACCTGTACGTCCGGATTTGCAACACACTGCGCTTTCAGCGCTTCAACAGCCTCTGCACACATGCGGATGCTCAGATCCGGGCGAGTCAGGTAGAGATTCTTGTCGCTAATTCCAGAGCGTACTTCCAGCAAACCCTGCGCCTCCACCCACCCGTCAGGCACTTCTTTCAGTACAATATCTTTCGAACGAGAGTGAGCGGACAGTAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAAcg                                                                                                                                                                                                           <  2:183194/274‑1 (MQ=255)                                                                                                        gAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAAt                                                                                                                                                                          >  2:17307/1‑300 (MQ=255)                                                                                                        gAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAAt                                                                                                                                                                          <  1:577322/300‑1 (MQ=255)                                                                                                             aaCGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTc                                                                                                                                                                     <  1:484691/300‑1 (MQ=255)                                                                                                                                   gCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAaca                                                                                                                                               >  2:600331/1‑300 (MQ=255)                                                                                                                                   gCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAaca                                                                                                                                               >  1:57363/1‑300 (MQ=255)                                                                                                                                   gCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTNCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCAACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAaca                                                                                                                                               >  2:545847/1‑300 (MQ=255)                                                                                                                                     aaCACACTACGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACAcc                                                                                                                                             <  2:477312/300‑1 (MQ=255)                                                                                                                                                            cAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCa                                                                                                                          >  2:44626/1‑296 (MQ=255)                                                                                                                                                                  cTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTa                                                                                                                >  1:93572/1‑300 (MQ=255)                                                                                                                                                                    tCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCCGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTaaa                                                                                                              >  2:252614/1‑300 (MQ=255)                                                                                                                                                                    tCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAANGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGAACGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTaaa                                                                                                              >  2:526408/1‑300 (MQ=255)                                                                                                                                                                    tCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTaaa                                                                                                              >  1:116873/1‑300 (MQ=255)                                                                                                                                                                    tCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGAACGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATATGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTaaa                                                                                                              >  2:349701/1‑300 (MQ=255)                                                                                                                                                                                 ggcggcCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCGGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAGTCCAGCGCACAGc                                                                                                 <  2:114436/300‑1 (MQ=255)                                                                                                                                                                                     gcCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTc                                                                                             >  2:398961/1‑300 (MQ=255)                                                                                                                                                                                     gcCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGAACGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTc                                                                                             >  1:401182/1‑300 (MQ=255)                                                                                                                                                                                         atatCCTGGCGAGTCGGTTAGAGATTTTTGTCGCTAATTTTAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTCCCGa                                                                                          <  2:635993/299‑1 (MQ=255)                                                                                                                                                                                         atatCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCg                                                                                           <  1:602972/298‑1 (MQ=255)                                                                                                                                                                                         atatCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGa                                                                                          <  2:650195/299‑1 (MQ=255)                                                                                                                                                                                           atCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATTGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAAAACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGt                                                                                       >  2:372556/1‑300 (MQ=255)                                                                                                                                                                                                    aGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCTGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGc                                                                              <  1:77031/300‑1 (MQ=255)                                                                                                                                                                                                    aGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGc                                                                              <  1:77040/300‑1 (MQ=255)                                                                                                                                                                                                         gttatagaTTTTTTTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTGTTTTCAGTACGATATCTTTCGAACGAGAGGGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAAGCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGcac                                                                         <  2:158458/295‑1 (MQ=255)                                                                                                                                                                                                         ggTATAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCACCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGcac                                                                         <  2:515215/300‑1 (MQ=255)                                                                                                                                                                                                                   tttGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTtggtggt                                                               <  2:441435/300‑1 (MQ=255)                                                                                                                                                                                                                                    agCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTg                                              <  1:453693/300‑1 (MQ=255)                                                                                                                                                                                                                                     gCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGa                                             >  1:237370/1‑300 (MQ=255)                                                                                                                                                                                                                                       gTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCTGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGTAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTTTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGAc                                            <  2:345769/299‑1 (MQ=255)                                                                                                                                                                                                                                        tACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGAACGGTACAAACGCGGGCAACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCCACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCAACGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGAc                                            >  2:321486/1‑298 (MQ=255)                                                                                                                                                                                                                                          cTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAANGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGgc                                         >  2:545795/1‑299 (MQ=255)                                                                                                                                                                                                                                           ttCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATATGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGgcgc                                       >  2:18153/1‑300 (MQ=255)                                                                                                                                                                                                                                                        tGCGCTGTCACCCACTCGTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGNGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAACCCAACGCACAGCTTTCCGAGGGTACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGGCGTGGGGGCCGCTTg                           <  2:542081/299‑1 (MQ=255)                                                                                                                                                                                                                                                           gcTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGGCGCGGGGGCCGCTTGTcc                        <  2:455383/299‑1 (MQ=255)                                                                                                                                                                                                                                                                             ggCACTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTg     <  1:729849/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              gCACTCCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGTCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTg     <  2:192094/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                aCTTCTTTCAGTACGATATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGt  >  2:680867/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                             GAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGCACGACCGGTACAAACGCGGGCCACGGTGCTGCGGCGCAGTTCTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGT  >  NC_000913/2556698‑2557268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑