Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,730,560	C→T	W69* (TGG→TAG)	xylA ←	xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,730,560	0	C	T	97.8%	289.0 / NA	92	W69* (TGG→TAG)	xylA	xylose isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (42/48);  minor base G (0/2);  total (42/50)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCCGCCCAGCTGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTT‑CAGTTGCTCAATTATGTTATTTC  >  NC_000913/3730264‑3730854                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                         ttGCCGCCCAGCTGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACg                                                                                                                                                                                                                                                                                                       <  2:646476/300‑1 (MQ=255)      gccCAGCTGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACCGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGAc                                                                                                                                                                                                                                                                                                    <  1:442864/298‑1 (MQ=255)      gccCAGCTGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGAc                                                                                                                                                                                                                                                                                                    <  1:442911/298‑1 (MQ=255)           gCTGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTaaa                                                                                                                                                                                                                                                                                             >  1:312890/1‑300 (MQ=255)             tGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACg                                                                                                                                                                                                                                                                                           <  1:113900/300‑1 (MQ=255)                   cTTCAGGATCTGGGTTTGTCGCCGCACCCGCGACGTAGCGAGGGTTTGTAGAGCAGTTGGCCGTTCACCACAGCAGCTTCACGCCGCTCTCTCATTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTAACCAGGCTGCTGCTACGGACGATTAAACGccccc                                                                                                                                                                                                                                                                                      >  2:400067/1‑299 (MQ=255)                   cTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCAAGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTACGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCACATGCGACATCTACTTTACGCTTCGCCAAAGCAAGTGCCTCAACAGGCTGCTGCTAAAGACGCTTAAACGCCCCca                                                                                                                                                                                                                                                                                     >  2:184509/1‑300 (MQ=255)                    ttCAGGATCTGGGTTCGTCGCCGCACCCTCGCCGTAGCGAGGGTTTGTAAAGCAGTCGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCAGTTGCGCAAAATTATTGACGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCTACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCcac                                                                                                                                                                                                                                                                                    <  2:198470/300‑1 (MQ=255)                    ttCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTGGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCcac                                                                                                                                                                                                                                                                                    <  2:198372/300‑1 (MQ=255)                     tCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCATGTGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCcac                                                                                                                                                                                                                                                                                    <  2:198285/299‑1 (MQ=255)                       aGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCacac                                                                                                                                                                                                                                                                                  <  1:268402/299‑1 (MQ=255)                       aGGATCTGGGTTCGTCGCCGCACCCGCGCCGGAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCacac                                                                                                                                                                                                                                                                                  <  1:279926/299‑1 (MQ=255)                        ggATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAACTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGTTGCTGCTACGGATGATTAAACGCCCCCacac                                                                                                                                                                                                                                                                                  <  1:267890/298‑1 (MQ=255)                              gggTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACata                                                                                                                                                                                                                                                                          >  1:471259/1‑300 (MQ=255)                                  tcgtcgCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCg                                                                                                                                                                                                                                                                      <  1:57936/300‑1 (MQ=255)                                                      tAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGgt                                                                                                                                                                                                                                                   >  2:642642/1‑299 (MQ=255)                                                       aGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGgtgt                                                                                                                                                                                                                                                 >  1:110149/1‑300 (MQ=255)                                                            gggTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAAGTATTGATGTACGCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAg                                                                                                                                                                                                                                            <  2:306494/300‑1 (MQ=255)                                                            gggTTGGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAg                                                                                                                                                                                                                                            <  2:421531/300‑1 (MQ=255)                                                             ggTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGcc                                                                                                                                                                                                                                              <  2:347763/297‑1 (MQ=255)                                                             ggTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAACAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCCACGCCAATGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCTCGTTCCCGCAAAAGGTGTGCCAGt                                                                                                                                                                                                                                           >  1:267504/1‑300 (MQ=255)                                                                             ggCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACg                                                                                                                                                                                                                           <  1:588592/300‑1 (MQ=255)                                                                                    ccccACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGc                                                                                                                                                                                                                    >  1:471505/1‑300 (MQ=255)                                                                                          agcagcTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTc                                                                                                                                                                                                               <  1:52560/299‑1 (MQ=255)                                                                                          agcagcTTCACGCCACTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGctc                                                                                                                                                                                                                  <  1:387510/296‑1 (MQ=255)                                                                                              gcTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCNCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATAc                                                                                                                                                                                                          >  1:148571/1‑300 (MQ=255)                                                                                              gcTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATAc                                                                                                                                                                                                          >  1:285141/1‑300 (MQ=255)                                                                                                ttCACGCCGCTCTCTTCTTGCTTGCCTGCCAGTACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTCCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGc                                                                                                                                                                                                        <  2:166099/300‑1 (MQ=255)                                                                                                        gcTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACCTCTCCGCCCCGTTCCAGCAGAAGGTGTGCAAGTAGCAGGCGGCAAAACGCAAGTGCTCTTACATACGCTTACCCa                                                                                                                                                                                                 >  2:384826/1‑299 (MQ=255)                                                                                                            tcttctTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAacac                                                                                                                                                                                             <  2:400441/299‑1 (MQ=255)                                                                                                            tcttctTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACAcc                                                                                                                                                                                            <  1:643498/300‑1 (MQ=255)                                                                                                             cttcttGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAANTGCGACATATGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACAcc                                                                                                                                                                                            >  2:541353/1‑299 (MQ=255)                                                                                                             cttcttGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCa                                                                                                                                                                                           >  1:465490/1‑300 (MQ=255)                                                                                                             cttcttGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTAACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGTGGCAGAACGCAAGTGCTCTTCCATACGCTTACCCAACACCa                                                                                                                                                                                           >  2:395641/1‑300 (MQ=255)                                                                                                                 ttgcttgcCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGtt                                                                                                                                                                                        <  2:137510/299‑1 (MQ=255)                                                                                                                              aGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAg                                                                                                                                                                          <  1:671799/300‑1 (MQ=255)                                                                                                                                    tCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGa                                                                                                                                                                       >  1:14880/1‑297 (MQ=255)                                                                                                                                                gcAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACggg                                                                                                                                                       <  1:642642/300‑1 (MQ=255)                                                                                                                                                       tattGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAgg                                                                                                                                                <  1:202510/300‑1 (MQ=255)                                                                                                                                                           gATGTACTCTTTTAACNACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGNATTGTAGTGACGGAATGCNGAAAGGGTTTgggggtt                                                                                                                                             >  1:616866/1‑294 (MQ=255)                                                                                                                                                           gATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGAttt                                                                                                                                            >  1:233219/1‑300 (MQ=255)                                                                                                                                                                           gacgCGCCCTCAGGGGAAACAGCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATaacg                                                                                                                             <  2:167414/299‑1 (MQ=255)                                                                                                                                                                                 cccTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTCTGAGCCTTCATAACGAACGCGa                                                                                                                      >  2:342412/1‑300 (MQ=255)                                                                                                                                                                                       ggggAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCTGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGc                                                                                                                <  2:662057/300‑1 (MQ=255)                                                                                                                                                                                       ggggAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGc                                                                                                                <  2:719001/300‑1 (MQ=255)                                                                                                                                                                                       ggggAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGc                                                                                                                >  1:230749/1‑300 (MQ=255)                                                                                                                                                                                       ggggAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCCTCATAACGAACGCGATCGAGc                                                                                                                >  2:63820/1‑300 (MQ=255)                                                                                                                                                                                       ggggAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACAACAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGCTTTTGAGCCTTCATAACGAACGCGATCGAGc                                                                                                                >  2:35050/1‑300 (MQ=255)                                                                                                                                                                                              cATCCACATCGTGGAANCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATNTTGAGCCTTCATAACGAANGCGATCGAGCTGGTCaa                                                                                                         >  1:626933/1‑300 (MQ=255)                                                                                                                                                                                              cATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCaa                                                                                                         >  1:130136/1‑300 (MQ=255)                                                                                                                                                                                                           gAAGCAATAAAATGGCACATGTAACTTGTGT‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCat                                                                                            <  2:624344/300‑1 (MQ=255)                                                                                                                                                                                                             aGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCATGCTGCTACTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCatat                                                                                          <  1:127570/300‑1 (MQ=255)                                                                                                                                                                                                             aGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCGACGGACGAGTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCatat                                                                                          <  1:127639/300‑1 (MQ=255)                                                                                                                                                                                                             aGCAAGAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCatat                                                                                          <  1:127557/300‑1 (MQ=255)                                                                                                                                                                                                                  tAAAATGGCACATGTAACCTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACACCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATACTCGCCCCGTTCCAGCATAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAGAATAGGCTTGCATATTGAAc                                                                                     <  2:201669/300‑1 (MQ=255)                                                                                                                                                                                                                    aaaTGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTc                                                                                   <  1:177169/300‑1 (MQ=255)                                                                                                                                                                                                                    aaaTGGCACATGGAACTTGTGG‑AAAAACTCAAATTCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTc                                                                                   <  1:177039/300‑1 (MQ=255)                                                                                                                                                                                                                           acaTGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATc                                                                            <  1:750058/300‑1 (MQ=255)                                                                                                                                                                                                                           acaTGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACACAAGTACTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATc                                                                            <  2:371174/300‑1 (MQ=255)                                                                                                                                                                                                                           acaTGTAACTT‑TGGTAAAAACTCAAATTCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGAGTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATc                                                                            <  2:87501/300‑1 (MQ=255)                                                                                                                                                                                                                            caTGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGCGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCa                                                                           >  1:188380/1‑300 (MQ=255)                                                                                                                                                                                                                            caTGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCt                                                                                                 >  1:735731/1‑278 (MQ=255)                                                                                                                                                                                                                            caTGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCt                                                                                                 <  2:735731/278‑1 (MQ=255)                                                                                                                                                                                                                            caTGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCa                                                                           >  1:188370/1‑300 (MQ=255)                                                                                                                                                                                                                            caTGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTACATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCa                                                                           >  1:573398/1‑300 (MQ=255)                                                                                                                                                                                                                             atgtagctttttg‑AAAAACTCAAATGCTACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCGGGCTGCTGCTACGGACGATGAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGAGGAAAAACGCAGGTGCTCTTCCATACGCCTACCCAACACCAGCTCGTCTGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCGTCATAACGAACGCGATCGAGCTGTTCAAAATAGGCTTGCATATTGAACTCCATAATc                                                                            <  2:87605/286‑1 (MQ=255)                                                                                                                                                                                                                                      gtgG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCcg                                                                 <  1:395641/300‑1 (MQ=255)                                                                                                                                                                                                                                                   aaaTTCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgatgga                                                      <  2:110149/299‑1 (MQ=255)                                                                                                                                                                                                                                                          aCATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTc                                               >  1:557931/1‑299 (MQ=255)                                                                                                                                                                                                                                                          aCATCTGCTTTACGCTTCGCCAACGCCAGTGCCTAACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTc                                               >  1:175561/1‑299 (MQ=255)                                                                                                                                                                                                                                                               tGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAat                                          <  1:81880/299‑1 (MQ=255)                                                                                                                                                                                                                                                                gCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAatat                                        >  2:447564/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAatat                                        >  1:237988/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATNGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCCTATTGAACTCCATAATCAGGTAATGCAGAGGGTGATGGGTGATGTCGTActat                                        >  2:225155/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     aCGCTTCGCCAACACCAGTGCCGCACCAGGCTGCTGCGACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAATGTGTGCCAGTAGCAGGCGGCAAAACGTAAGTGCTCTTCCCTACGCTTACCCAACACCAGTGCGTCGGGATTGTAGTGACTGAATGCT‑AACGGGTTTGAGGACTTGGAGCCTTCATAATGAACGCGGTCGAGCTGGTCAAAATATGCTTGCATATTGAACTTCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGc                                   <  2:407389/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         tttgCCAACGCCAGTGCCTCACCAGGCTGCTGCTACTGACGATTAAACGCCCCCACACCAAACATATCCTCCCCGTGCCAGCAGAAGGTGTGCCAGTAGCGGGCGGCAAAACGCAAGTGCTCTTc                                                                                                                                                                                                               <  2:203717/122‑1 (MQ=255)                                                                                                                                                                                                                                                                          tCGCCAACGCCANTGCCTCACCAGGCTGCTGNTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGNTGTCGTAATATTGGGc                                   <  1:617390/295‑1 (MQ=255)                                                                                                                                                                                                                                                                            gCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCt                            >  1:345374/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               aaCGCCAGTGCCGCACCAGGCGGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCTGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGTTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCCTT‑c                        <  2:312890/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 cGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGAAGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCttcca                        >  2:117873/1‑296 (MQ=255)                                                                                                                                                                                                                                                                                  gCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCACACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCAGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCttt                          >  2:281796/1‑296 (MQ=255)                                                                                                                                                                                                                                                                                     aGTGCCTCACCATGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAATTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTT‑CAg                      <  1:716669/296‑1 (MQ=255)                                                                                                                                                                                                                                                                                           tCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAAt                                                                             >  1:705291/1‑236 (MQ=255)                                                                                                                                                                                                                                                                                           tCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAAt                                                                             >  1:705545/1‑236 (MQ=255)                                                                                                                                                                                                                                                                                           tCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAAt                                                                             <  2:705291/236‑1 (MQ=255)                                                                                                                                                                                                                                                                                           tCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAAt                                                                             <  2:705545/236‑1 (MQ=255)                                                                                                                                                                                                                                                                                           tCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTT‑CAGTTGCTCAAtt            >  1:92320/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           tCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTT‑AAGTTGCTCAAtt            >  2:308362/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           tCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCAGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTT‑CAGTTGCTCAAtt            >  1:93333/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           tCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCAATACGCTTACCCAACACCAGTTCGTAGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCAGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTT‑CAGTTGATCAAtt            >  2:320774/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            cACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGGTGGATGATGTCGTAATATTGGGCATTCCCTTTACA‑TTGCTCAATTa           >  2:45307/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 ggCTGCTGTTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTT‑CAGTTGCTCAATTATGTTa      <  2:181009/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     gctgctACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTT‑CAGTTGCTCAATTATGTTATTTc  >  1:642181/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                         TTGCCGCCCAGCTGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGG‑AAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCT‑AACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTT‑CAGTTGCTCAATTATGTTATTTC  >  NC_000913/3730264‑3730854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑