Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	547,891	C→T	L404L (CTC→CTT)	fdrA →	putative oxamate carbamoyltransferase FdrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	547,891	0	C	T	100.0%	189.0 / NA	53	L404L (CTC→CTT)	fdrA	putative oxamate carbamoyltransferase FdrA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/23);  total (30/23)

ACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAA  >  NC_000913/547642‑548185                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                       aCGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCAACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGAGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCtt                                                                                                                                                                                                                                                      >  2:209957/1‑300 (MQ=255)      aCGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGt                                                                                                                                                                                                                                                  >  2:492298/1‑299 (MQ=255)      aCGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCAACAAGATTATTGACCTCGGCGATGATTTCTAAACCGTCGGGCGTCCCCATCCGATGATCGAACCAAACTTACGCACCCAGTTAATTGCCGATTTTGGAGCTAAACCGCAAGTGCGCGTGTTGCGGCTTGATGTCGTGGTTGGCTTCGGt                                                                                                                                                                                                                                                  >  2:492509/1‑299 (MQ=255)         cAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGa                                                                                                                                                                                                                                              >  1:291808/1‑300 (MQ=255)         cAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCAACAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGa                                                                                                                                                                                                                                              >  1:659770/1‑300 (MQ=255)                 ccTGTCAGCAGCGGATNTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTNCTTGATGTCGTGATTGGCNTCGGTGCGACCGCCGAt                                                                                                                                                                                                                                      >  1:632297/1‑300 (MQ=255)                   tGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATTGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATc                                                                                                                                                                                                                                     <  1:380379/299‑1 (MQ=255)                           gcagATTTATTTGCTGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCTGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGACCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCt                                                                                                                                                                                                                            <  2:668739/297‑1 (MQ=255)                             ggATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTc                                                                                                                                                                                                                           >  2:632431/1‑299 (MQ=255)                                   atttGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTg                                                                                                                                                                                                                    <  1:661554/300‑1 (MQ=255)                                              gTATACCGGCGGTACGCTGGCTGCAGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGc                                                                                                                                                                                                          >  2:209121/1‑299 (MQ=255)                                                      gcggTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCTATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCt                                                                                                                                                                                                 >  1:99420/1‑300 (MQ=255)                                                          tACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACTTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGc                                                                                                                                                                                             <  1:7594/300‑1 (MQ=255)                                                          tACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACAAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGc                                                                                                                                                                                             <  1:403025/300‑1 (MQ=255)                                                                    gCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTa                                                                                                                                                                                   >  1:504259/1‑300 (MQ=255)                                                                       gAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGAt                                                                                                                                                                                <  1:504307/300‑1 (MQ=255)                                                                               gggATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATTGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAAcc                                                                                                                                                                        <  2:478035/300‑1 (MQ=255)                                                                                    tACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGt                                                                                                                                                                   <  1:563882/300‑1 (MQ=255)                                                                                        tGCCGGACACCTTTGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCTTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGc                                                                                                                                                               <  1:64482/300‑1 (MQ=255)                                                                                        tGCCGGACACCTTGGCGTGGAAGCCGACGGGACCCATCAACATTGCATGATGCGGGACGCCGATAGCCACCAGATTATGGACCTCGGCGATGATTTCTACACCTTCGTGCGTCCCCATCCGATAATCGACCCAACCTTACGCAACCAGTTAATTACCGATCTTGGCGCTAAACCCCAAGTTCGCGTGTGGCTGCTTGGTTTCTTGATTGGCTTCGGTGCGACCGCTGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCTTGTGCCGCGCGTTTAGATAATCAACCACTGTAtcc                                                                                                                                                               <  2:280711/300‑3 (MQ=255)                                                                                        tGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATNGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGc                                                                                                                                                               <  1:535268/300‑1 (MQ=255)                                                                                         gCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGcc                                                                                                                                                              <  2:262485/300‑1 (MQ=255)                                                                                         gCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGcc                                                                                                                                                              <  1:276604/300‑1 (MQ=255)                                                                                            ggACACCTTGGCGTGGAAGCCGACGATACCCATCAACATTGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCact                                                                                                                                                           >  1:199725/1‑298 (MQ=255)                                                                                            ggACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCAt                                                                                                                                                            <  1:352343/299‑1 (MQ=255)                                                                                                                 gacgaTACCCATCAACATGGCATGATGCTGGACGCCGATAGCAACCAGACTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCACATCCGATGATCGACCCAACCTTACGCAAACAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGTCTGTGTCGCGTGTTTAGATAATTAACCACTGTATGCCATTGCCCCGGTGCCAGGCACTGaa                                                                                                                                      >  2:54521/1‑300 (MQ=255)                                                                                                                  acgaTACCCATCAACATTGCATGATGCTGGACGCCGATAGCCACCAGATTATCGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGaa                                                                                                                                      <  2:311944/299‑1 (MQ=255)                                                                                                                        cccATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCCCGGTGACAGGCACTGAACGTGAcc                                                                                                                               >  1:729495/1‑300 (MQ=255)                                                                                                                        cccATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGAcc                                                                                                                               >  1:729520/1‑300 (MQ=255)                                                                                                                             cAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTAGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATAAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCaa                                                                                                                          >  2:382398/1‑300 (MQ=255)                                                                                                                               aCATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGaa                                                                                                                                      <  1:469571/286‑1 (MQ=255)                                                                                                                               aCATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAAt                                                                                                                         >  1:304273/1‑299 (MQ=255)                                                                                                                               aCATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCAGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGaa                                                                                                                                      >  2:469571/1‑286 (MQ=255)                                                                                                                               aCATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTACTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAAt                                                                                                                         >  1:345424/1‑299 (MQ=255)                                                                                                                                               gACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATc                                                                                                        <  1:477239/300‑1 (MQ=255)                                                                                                                                                         gcccccaGATTATTGACCTCGGCGCTGATTTCTACACCGTAGGGCGTCCCCATCAGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGTGACCGCCGATCCTGCCGCCTAGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACAAATGTATGACATTGCCACGGTGACAGGCCCTGAACGTGACCCGCAATGCCGCTAGTAGCAAATCGCCAAGCt                                                                                                >  2:406115/5‑298 (MQ=255)                                                                                                                                                                         ccTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGc                                                                              >  1:611196/1‑300 (MQ=255)                                                                                                                                                                                     tttCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTc                                                                  >  1:501882/1‑300 (MQ=255)                                                                                                                                                                                             ccGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTAt                                                                                                                                                                 >  1:407686/1‑197 (MQ=255)                                                                                                                                                                                             ccGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTAt                                                                                                                                                                 <  2:407686/197‑1 (MQ=255)                                                                                                                                                                                                                        aCCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGGTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGAAAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCAACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGTGGTAGCGTTaa                               >  2:198976/1‑300 (MQ=255)                                                                                                                                                                                                                        aCCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGTGGCAGCGTTaa                               >  2:495073/1‑300 (MQ=255)                                                                                                                                                                                                                        aCCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTaa                               >  1:365660/1‑300 (MQ=255)                                                                                                                                                                                                                        aCCCAACCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTaa                               >  2:436393/1‑300 (MQ=255)                                                                                                                                                                                                                            aaCCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCACAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGCGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAAtt                             <  2:658689/298‑1 (MQ=255)                                                                                                                                                                                                                             aCCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCACTGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTCAATTc                            >  2:739468/1‑298 (MQ=255)                                                                                                                                                                                                                             aCCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCAt                          >  2:477675/1‑300 (MQ=255)                                                                                                                                                                                                                             aCCTTACGCAACCAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCATGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTATATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCGGCAAATCGCCACGCTGGAAGATGCGGGGATTGTGGTCGTGAGTTCGCTACCGGAAGCCACCTTGTTGGCGGCAGTGTTAATTCAt                          >  2:406149/1‑300 (MQ=255)                                                                                                                                                                                                                                 tACGCAACGAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATcc                        <  1:639750/298‑1 (MQ=255)                                                                                                                                                                                                                                        ccAGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCCCGGTGACAGGCACTGAACGTGACCCGCCATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGCTTGCGGTCGTGAGTTTGCTAACGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCtctc                  >  2:254062/1‑297 (MQ=255)                                                                                                                                                                                                                                          aGTTAATTGCCGATCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGcc               <  1:222522/298‑1 (MQ=255)                                                                                                                                                                                                                                                      aTCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCaa  <  2:729495/299‑1 (MQ=255)                                                                                                                                                                                                                                                      aTCTTGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCaa  <  2:729520/299‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                       ACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATTTCTACACCGTCGGGCGTCCCCATCCGATGATCGACCCAACCTTACGCAACCAGTTAATTGCCGATCTCGGCGCTAAACCGCAAGTGCGCGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAA  >  NC_000913/547642‑548185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑