breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 860 C→T A175V (GCG→GTG)  thrA → fused aspartate kinase/homoserine dehydrogenase 1
RA 10,564 C→T intergenic (‑70/+79) satP ← / ← yaaW acetate/succinate:H(+) symporter/putative enzyme‑specific chaperone YaaW
RA 23,291 C→T L301F (CTT→TTT)  ileS → isoleucine‑‑tRNA ligase
RA 25,114 C→T T908T (ACC→ACT)  ileS → isoleucine‑‑tRNA ligase
RA 47,652 G→A G136E (GGG→GAG)  kefF → regulator of KefC‑mediated potassium transport and quinone oxidoreductase
JC JC 57,276 IS2 (+) +5 bp intergenic (‑167/‑84) lptD ← / → djlA lipopolysaccharide assembly protein LptD/co‑chaperone protein DjlA
RA 69,531 G→A S173F (TCT→TTT)  araB ← ribulokinase
RA 71,134 G→A G250S (GGT→AGT)  araC → DNA‑binding transcriptional dual regulator AraC
RA 81,556 G→A L135L (CTG→TTG)  leuB ← 3‑isopropylmalate dehydrogenase
RA 148,424 C→T P124P (CCG→CCA)  panC ← pantothenate synthetase
MC JC 257,908 Δ776 bp insB9–[crl] insB9, insA9, [crl]
RA 311,511 G→A intergenic (‑175/‑601) matA ← / → ykgL DNA‑binding transcriptional dual regulator MatA/uncharacterized protein YkgL
RA 378,391 C→T intergenic (‑23/+71) frmB ← / ← frmA S‑formylglutathione hydrolase FrmB/S‑(hydroxymethyl)glutathione dehydrogenase
RA 390,948 C→T pseudogene (698/1458 nt) yaiT → putative autotransporter YaiT
RA 418,613 C→T A242V (GCG→GTG)  phoR → sensor histidine kinase PhoR
RA 432,880 C→T intergenic (‑29/‑122) yajI ← / → nrdR putative lipoprotein YajI/NrdR transcriptional repressor
RA 438,623 C→T A519T (GCG→ACG)  dxs ← 1‑deoxy‑D‑xylulose‑5‑phosphate synthase
RA 441,293 C→T E17E (GAG→GAA)  xseB ← exodeoxyribonuclease VII subunit XseB
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
JC JC 568,449 IS2 (–) +5 bp coding (135‑139/333 nt) emrE → multidrug/betaine/choline efflux transporter EmrE
RA 631,633 G→A W580* (TGG→TGA)  cstA → pyruvate transporter CstA
RA 722,194 G→A L741L (CTG→TTG)  kdpD ← sensor histidine kinase KdpD
RA 736,420 C→T intergenic (+201/‑25) ybfC → / → ybfQ uncharacterized protein YbfC/inactive transposase YbfQ
RA 781,595 G→A noncoding (19/76 nt) lysQ → tRNA‑Lys
RA 987,152 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
RA 1,196,220 C→T H366H (CAC→CAT)  icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT)  icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT)  icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC)  icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG)  icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT)  icd → isocitrate dehydrogenase
RA 1,290,881 C→T L214F (CTT→TTT)  rssB → regulator of RpoS
MC JC 1,294,035 Δ141 bp [tdk] [tdk]
JC 1,303,073 Δ12 bp coding (175‑186/921 nt) oppB → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
RA 1,312,633 C→T A86A (GCG→GCA)  yciB ← inner membrane protein
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,353,758 T→A D254V (GAT→GTT)  sapC ← putrescine ABC exporter membrane protein SapC
RA 1,494,388 Δ1 bp coding (684/924 nt) ydcI ← DNA‑binding transcriptional dual regulator YdcI
RA 1,517,508 G→T V40V (GTG→GTT)  ortT → orphan toxin OrtT
RA 1,531,250 C→T intergenic (‑746/‑566) yncO ← / → ydcC protein YncO/H repeat‑associated putative transposase YdcC
RA 1,556,938 C→T A105V (GCG→GTG)  osmC → osmotically inducible peroxiredoxin OsmC
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,667,887 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,744,751 Δ1 bp coding (1295/1374 nt) mdtK → multidrug efflux pump MdtK
JC JC 1,792,772 IS2 (–) +5 bp intergenic (‑41/+33) nlpC ← / ← btuD NlpC/P60 family lipoprotein NlpC/vitamin B12 ABC transporter ATP binding subunit
RA 1,978,243 G→T intergenic (‑46/+275) flhD ← / ← insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
RA 2,003,264 C→T E115K (GAA→AAA)  fliC ← flagellar filament structural protein
RA 2,032,423 G→A V219V (GTC→GTT)  yedJ ← putative HD superfamily phosphohydrolase YedJ
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,179,326 G→A G169E (GGA→GAA)  yegT → putative transporter YegT
RA 2,242,121 A→G S283P (TCG→CCG)  yeiB ← DUF418 domain‑containing protein YeiB
JC JC 2,269,876 IS5 (+) +4 bp intergenic (+309/‑100) lpxT → / → mepS lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
RA 2,278,650 G→A P371S (CCG→TCG)  bcr ← multidrug efflux pump Bcr
RA 2,313,736 C→T S83S (TCC→TCT)  rcsD → RcsD phosphotransferase
RA 2,316,221 C→T V15V (GTC→GTT)  rcsB → DNA‑binding transcriptional activator RcsB
RA 2,316,753 C→T L193L (CTG→TTG)  rcsB → DNA‑binding transcriptional activator RcsB
RA 2,317,233 C→T D882N (GAT→AAT)  rcsC ← histidine kinase RcsC
RA 2,317,463 C→T S805N (AGC→AAC)  rcsC ← histidine kinase RcsC
JC JC 2,319,850 IS2 (+) +5 bp coding (23‑27/2850 nt) rcsC ← histidine kinase RcsC
RA 2,320,552 C→T G170G (GGC→GGT)  atoS → sensor histidine kinase AtoS
RA 2,321,526 C→T A495V (GCC→GTC)  atoS → sensor histidine kinase AtoS
RA 2,324,745 C→T R213W (CGG→TGG)  atoA → acetyl‑CoA:acetoacetyl‑CoA transferase subunit beta
RA 2,327,447 C→T V233I (GTC→ATC)  yfaP ← DUF2135 domain‑containing protein YfaP
RA 2,397,444 G→A Q908* (CAA→TAA)  nuoG ← NADH:quinone oxidoreductase subunit G
JC JC 2,406,780 IS1 (–) +8 bp intergenic (‑139/‑774) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
RA 2,480,474 G→A G425R (GGA→AGA)  dsdA → D‑serine ammonia‑lyase
MC JC 2,558,699 Δ6,790 bp intZ–[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
JC JC 2,666,074 IS1 (+) +9 bp coding (640‑648/1281 nt) csiE → stationary phase‑inducible protein CsiE
RA 2,784,599 G→A S138F (TCT→TTT)  ypjC ← DUF5507 domain‑containing protein YpjC
RA 2,789,285 G→A G101S (GGT→AGT)  glaH → glutarate dioxygenase GlaH
RA 2,794,383 G→A G44D (GGC→GAC)  gabP → 4‑aminobutanoate:H(+) symporter
RA 2,799,156 G→A intergenic (‑661/‑8) stpA ← / → alaE DNA‑binding transcriptional repressor StpA with RNA chaperone activity/L‑alanine exporter
RA 2,799,342 G→A G60D (GGT→GAT)  alaE → L‑alanine exporter
RA 2,803,246 G→A A633A (GCG→GCA)  nrdE → ribonucleoside‑diphosphate reductase 2 subunit alpha
RA 2,815,005 G→A P479S (CCG→TCG)  gshA ← glutamate‑‑cysteine ligase
RA 2,816,433 G→A P3S (CCG→TCG)  gshA ← glutamate‑‑cysteine ligase
RA 2,827,260 T→C V290A (GTC→GCC)  srlE → sorbitol‑specific PTS enzyme IIBC1 component
RA 2,845,115 G→A P453S (CCG→TCG)  hycE ← formate hydrogenlyase subunit HycE
RA 2,856,061 G→A G575E (GGG→GAG)  fhlA → DNA‑binding transcriptional activator FhlA
RA 2,858,682 G→A E530E (GAG→GAA)  mutS → DNA mismatch repair protein MutS
RA 2,862,253 C→T A275V (GCC→GTC)  ygbJ → putative L‑threonate dehydrogenase
RA 2,864,013 C→T P141S (CCG→TCG)  ygbL → putative 3‑oxo‑tetronate 4‑phosphate decarboxylase YgbL
RA 2,871,025 G→A P94S (CCG→TCG)  truD ← tRNA pseudouridine(13) synthase
RA 2,878,239 G→A intergenic (+621/+330) iap → / ← cas2 alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2
RA 2,885,029 G→A L731L (CTG→TTG)  cas3 ← CRISPR‑associated endonuclease/helicase Cas3
RA 2,889,826 T→C T92A (ACC→GCC)  cysI ← sulfite reductase, hemoprotein subunit
RA 2,894,133 G→A W72* (TGG→TAG)  ygcO → putative 4Fe‑4S cluster‑containing protein
RA 2,899,661 G→A L205L (CTG→TTG)  ygcW ← putative deoxygluconate dehydrogenase
RA 2,926,071 C→T R242C (CGC→TGC)  queF → 7‑cyano‑7‑deazaguanine reductase
RA 2,930,384 C→T A270V (GCA→GTA)  sdaB → L‑serine deaminase II
RA 2,935,697 C→T A38A (GCC→GCT)  fucI → L‑fucose isomerase
RA 2,945,513 C→T T110T (ACG→ACA)  tcdA ← tRNA threonylcarbamoyladenosine dehydratase
RA 2,947,323 C→T intergenic (‑145/‑64) mltA ← / → metZ membrane‑bound lytic murein transglycosylase A/tRNA‑Met
RA 2,949,648 C→T A136V (GCC→GTC)  argA → N‑acetylglutamate synthase
RA 2,949,800 C→T L187L (CTG→TTG)  argA → N‑acetylglutamate synthase
RA 2,953,166 C→T P946P (CCG→CCA)  recB ← exodeoxyribonuclease V subunit RecB
RA 2,958,080 C→T V269I (GTA→ATA)  ptrA ← protease 3
RA 2,966,838 C→T E533K (GAG→AAG)  ptsP ← phosphoenolpyruvate‑protein phosphotransferase PtsP
RA 2,967,877 C→T W186* (TGG→TGA)  ptsP ← phosphoenolpyruvate‑protein phosphotransferase PtsP
RA 2,983,800 C→T G109S (GGT→AGT)  kduI ← 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
RA 2,984,177 C→T intergenic (‑53/+234) kduI ← / ← yqeF 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase/putative acyltransferase
RA 2,985,807 C→T intergenic (‑215/‑40) yqeF ← / → yqeG putative acyltransferase/putative transporter YqeG
RA 2,986,436 C→T S197L (TCG→TTG)  yqeG → putative transporter YqeG
RA 2,987,975 C→T T147I (ACC→ATC)  yqeH → putative LuxR family transcriptional regulator YqeH
RA 2,990,087 C→T E92K (GAA→AAA)  yqeK ← protein YqeK
RA 2,994,899 C→T intergenic (+1043/+1473) ygeI → / ← insD4 protein YgeI/IS2 insertion element protein InsB
RA 3,008,693 C→T A395V (GCT→GTT)  ygeX → 2,3‑diaminopropionate ammonia‑lyase
RA 3,009,580 G→A E273K (GAA→AAA)  ygeY → putative peptidase YgeY
RA 3,010,747 G→A L240L (CTG→CTA)  hyuA → phenylhydantoinase
RA 3,012,153 G→A A231A (GCG→GCA)  yqeA → putative amino acid kinase YqeA
RA 3,022,051 G→A G246S (GGT→AGT)  xdhD → fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
RA 3,042,914 C→T A79T (GCA→ACA)  yqfA ← hemolysin‑III family protein YqfA
RA 3,049,690 C→T A326A (GCG→GCA)  gcvT ← aminomethyltransferase
RA 3,056,165 C→T noncoding (183/183 nt) ssrS → 6S RNA
RA 3,057,590 C→T S274N (AGC→AAC)  serA ← phosphoglycerate dehydrogenase
RA 3,061,808 C→T T320I (ACC→ATC)  scpA → methylmalonyl‑CoA mutase
RA 3,062,319 C→T A490A (GCC→GCT)  scpA → methylmalonyl‑CoA mutase
RA 3,062,736 C→T R629R (CGC→CGT)  scpA → methylmalonyl‑CoA mutase
RA 3,071,691 C→T G311D (GGC→GAC)  pgk ← phosphoglycerate kinase
RA 3,073,577 C→T A39T (GCG→ACG)  epd ← D‑erythrose‑4‑phosphate dehydrogenase
RA 3,076,412 C→T V349M (GTG→ATG)  yggP ← putative zinc‑binding dehydrogenase YggP
RA 3,082,464 C→T F184F (TTC→TTT)  loiP → metalloprotease LoiP
RA 3,087,433 C→T T243I (ACT→ATT)  metK → methionine adenosyltransferase
RA 3,093,260 C→T S108S (TCC→TCT)  yqgE → DUF179 domain‑containing protein YqgE
RA 3,099,265 C→T A101T (GCG→ACG)  yggM ← DUF1202 domain‑containing protein YggM
RA 3,099,484 C→T E28K (GAA→AAA)  yggM ← DUF1202 domain‑containing protein YggM
RA 3,100,557 C→T V58I (GTT→ATT)  ansB ← L‑asparaginase 2
RA 3,112,243 C→T K269K (AAG→AAA)  yghF ← putative type II secretion system C‑type protein YghF
RA 3,114,520 C→T intergenic (‑168/+30) pppA ← / ← yghJ prepilin peptidase/putative lipoprotein YghJ
RA 3,160,906 C→T A81T (GCC→ACC)  ygiQ ← radical SAM superfamily protein YgiQ
RA 3,164,048 C→T M642I (ATG→ATA)  parC ← DNA topoisomerase IV subunit A
RA 3,167,365 C→T W118* (TGG→TGA)  ygiS ← putative deoxycholate binding periplasmic protein
RA 3,173,521 C→T A626T (GCG→ACG)  parE ← DNA topoisomerase IV subunit B
RA 3,175,472 C→T D179N (GAT→AAT)  yqiA ← esterase YqiA
RA 3,175,628 C→T V127I (GTC→ATC)  yqiA ← esterase YqiA
RA 3,190,892 G→A M87I (ATG→ATA)  yqiI → putative fimbrial protein YqiI
RA 3,193,282 C→T A140V (GCC→GTC)  yqiK → flotillin family inner membrane protein YqiK
RA 3,215,106 C→T W123* (TGG→TAG)  mug ← stationary phase mismatch/uracil DNA glycosylase
RA 3,218,479 C→T A200T (GCA→ACA)  aer ← aerotaxis receptor
RA 3,220,489 C→T T332T (ACC→ACT)  patA → putrescine aminotransferase
RA 3,232,319 C→T L219F (CTC→TTC)  fadH → 2,4‑dienoyl‑CoA reductase
RA 3,240,171 G→A G76G (GGG→GGA)  sstT → serine/threonine:Na(+) symporter
RA 3,243,005 C→T A104T (GCA→ACA)  uxaA ← D‑altronate dehydratase
RA 3,248,308 C→T P179L (CCG→CTG)  yqjA → DedA family protein YqjA
RA 3,248,681 C→T D81D (GAC→GAT)  mzrA → modulator protein MzrA
RA 3,251,737 C→T A238A (GCC→GCT)  yqjG → glutathionyl‑hydroquinone reductase YqjG
RA 3,254,120 C→T G32E (GGA→GAA)  yhaJ ← DNA‑binding transcriptional activator YhaJ
RA 3,254,853 C→T P179S (CCA→TCA)  yhaK → bicupin‑related protein
RA 3,255,992 G→A S220S (AGC→AGT)  cyuA ← putative L‑cysteine desulfidase CyuA
RA 3,261,943 G→A P159L (CCG→CTG)  tdcE ← 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4
RA 3,268,852 G→A G146G (GGG→GGA)  yhaC → uncharacterized protein YhaC
RA 3,268,970 A→G T186A (ACA→GCA)  yhaC → uncharacterized protein YhaC
RA 3,273,052 C→T G169D (GGC→GAC)  garL ← alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase
RA 3,276,711 C→T T477I (ACC→ATC)  garD → GarD
RA 3,478,611 C→T G61E (GGG→GAG)  yheV ← DUF2387 domain‑containing protein YheV
RA 3,481,609 G→A G107G (GGG→GGA)  yheS → putative ATP‑binding protein YheS
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,486,450 C→T P111S (CCG→TCG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,486,873 C→T P28L (CCC→CTC)  yhfK → putative transporter YhfK
RA 3,488,712 C→T A641V (GCC→GTC)  yhfK → putative transporter YhfK
RA 3,493,759 C→T intergenic (+10/‑252) tsgA → / → nirB putative transporter TsgA/nitrite reductase (NADH) large subunit
RA 3,518,251 G→A A79V (GCT→GTT)  aroB ← 3‑dehydroquinate synthase
RA 3,519,801 G→A V301V (GTC→GTT)  hofQ ← DNA utilization protein HofQ
RA 3,538,195 G→A A271T (GCG→ACG)  yhgF → putative RNA‑binding protein YhgF
RA 3,538,357 G→A G325R (GGA→AGA)  yhgF → putative RNA‑binding protein YhgF
RA 3,539,759 G→A intergenic (+53/‑404) yhgF → / → feoA putative RNA‑binding protein YhgF/ferrous iron transport protein A
RA 3,548,459 C→T E538K (GAG→AAG)  malQ ← 4‑alpha‑glucanotransferase
RA 3,550,402 C→T G691D (GGC→GAC)  malP ← maltodextrin phosphorylase
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,563,694 C→T intergenic (+176/+30) glpD → / ← yzgL aerobic glycerol 3‑phosphate dehydrogenase/putative uncharacterized protein YzgL
MC JC 3,682,261 Δ34 bp coding (1154‑1187/1287 nt) dctA ← C4 dicarboxylate/orotate:H(+) symporter
JC JC 3,737,137 IS2 (+) +5 bp coding (37‑41/825 nt) bax ← putative glycoside hydrolase Bax
RA 3,796,966 C→T L7F (CTT→TTT)  waaL → O‑antigen ligase
RA 3,814,113 C→T P62L (CCT→CTT)  dut → dUTP diphosphatase
RA 3,933,450 C→T P34S (CCC→TCC)  rbsD → D‑ribose pyranase
RA 3,934,173 C→T R132R (CGC→CGT)  rbsA → ribose ABC transporter ATP binding subunit
RA 3,941,568 G→A intergenic (‑241/‑240) yieP ← / → rrsC DNA‑binding transcriptional regulator YieP/16S ribosomal RNA
RA 3,942,034 G→A noncoding (227/1542 nt) rrsC → 16S ribosomal RNA
RA 4,061,400 C→T A79V (GCC→GTC)  yihM → putative TIM barrel domain‑containing protein YihM
JC JC 4,123,923 IS2 (–) +5 bp coding (530‑534/1026 nt) cytR ← DNA‑binding transcriptional repressor CytR
JC JC 4,135,180 IS2 (–) +5 bp coding (1346‑1350/2181 nt) katG → catalase/hydroperoxidase HPI
RA 4,146,389 (T)6→7 coding (132/879 nt) pflC → putative pyruvate formate‑lyase 2 activating enzyme PflC
RA 4,214,131 C→T intergenic (‑8/‑149) yjaB ← / → metA peptidyl‑lysine N‑acetyltransferase YjaB/homoserine O‑succinyltransferase
RA 4,218,867 C→T P91L (CCG→CTG)  aceK → isocitrate dehydrogenase kinase/phosphatase
RA 4,226,385 C→T P853L (CCG→CTG)  metH → cobalamin‑dependent methionine synthase
RA 4,250,729 C→T intergenic (+255/‑1777) malM → / → ubiC maltose regulon periplasmic protein/chorismate lyase
RA 4,253,539 C→T A175V (GCC→GTC)  ubiA → 4‑hydroxybenzoate octaprenyltransferase
RA 4,259,194 G→A intergenic (+73/‑43) dinF → / → yjbJ DNA damage‑inducible protein F/putative stress response protein YjbJ
RA 4,270,229 G→A intergenic (+102/‑9) aphA → / → yjbQ acid phosphatase/phosphotransferase/PF01894 family protein YjbQ
RA 4,277,732 G→A A88A (GCG→GCA)  soxR → DNA‑binding transcriptional dual regulator SoxR
RA 4,286,371 G→A P334L (CCC→CTC)  acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,286,403 G→A C323C (TGC→TGT)  acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,290,944 G→A V157M (GTG→ATG)  nrfD → putative menaquinol‑cytochrome c reductase subunit NrfD
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,298,639 G→A A243V (GCG→GTG)  fdhF ← formate dehydrogenase H
RA 4,302,032 G→A D349D (GAC→GAT)  mdtO ← putative multidrug efflux pump subunit MdtO
RA 4,302,462 G→A A206V (GCA→GTA)  mdtO ← putative multidrug efflux pump subunit MdtO
RA 4,303,109 G→A A334V (GCT→GTT)  mdtN ← putative multidrug efflux pump membrane fusion protein
RA 4,306,957 G→A A281A (GCC→GCT)  alsK ← D‑allose kinase
RA 4,310,026 C→T E319K (GAA→AAA)  alsA ← D‑allose ABC transporter ATP binding subunit
RA 4,315,749 C→T A112T (GCG→ACG)  phnN ← ribose 1,5‑bisphosphate phosphokinase
RA 4,316,019 C→T E22K (GAA→AAA)  phnN ← ribose 1,5‑bisphosphate phosphokinase
RA 4,316,390 C→T G277S (GGC→AGC)  phnM ← RPnTP hydrolase
RA 4,316,898 C→T R107R (CGG→CGA)  phnM ← RPnTP hydrolase
RA 4,317,597 C→T R100H (CGC→CAC)  phnL ← methylphosphonate degradation complex subunit PhnL
RA 4,317,641 C→T V85V (GTG→GTA)  phnL ← methylphosphonate degradation complex subunit PhnL
RA 4,317,944 C→T intergenic (‑49/+62) phnL ← / ← phnK methylphosphonate degradation complex subunit PhnL/carbon‑phosphorus lyase subunit PhnK
RA 4,318,776 C→T E277E (GAG→GAA)  phnJ ← carbon‑phosphorus lyase core complex subunit PhnJ
RA 4,319,494 C→T G38D (GGC→GAC)  phnJ ← carbon‑phosphorus lyase core complex subunit PhnJ
RA 4,325,150 G→A R6C (CGT→TGT)  phnC ← phosphonate/phosphate ABC transporter ATP binding subunit
RA 4,326,061 G→A intergenic (‑320/+338) yjdN ← / ← yjdM PF06983 family protein YjdN/zinc ribbon domain‑containing protein YjdM
RA 4,329,790 G→A G144D (GGT→GAT)  rdcB → regulator of diguanylate cyclase RdcB
RA 4,336,958 C→T G25E (GGG→GAG)  adiC ← arginine:agmatine antiporter
RA 4,339,222 C→T A434T (GCG→ACG)  adiA ← arginine decarboxylase, degradative
RA 4,342,807 C→T C299C (TGC→TGT)  melA → alpha‑galactosidase
RA 4,353,441 C→T G426D (GGT→GAT)  lysU ← lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase
RA 4,360,665 C→T A424T (GCG→ACG)  cadC ← DNA‑binding transcriptional activator CadC
RA 4,363,741 C→T W434* (TGG→TGA)  dsbD ← thiol‑disulfide exchange protein DsbD
RA 4,368,175 C→T M51I (ATG→ATA)  aspA ← aspartate ammonia‑lyase
RA 4,369,251 C→T V388I (GTC→ATC)  yjeH ← L‑methionine/branched chain amino acid exporter
RA 4,377,648 C→T P25P (CCG→CCA)  blc ← outer membrane lipoprotein Blc
RA 4,380,790 C→T G510D (GGC→GAC)  frdA ← fumarate reductase flavoprotein subunit
RA 4,380,900 C→T M473I (ATG→ATA)  frdA ← fumarate reductase flavoprotein subunit
RA 4,382,616 C→T intergenic (‑298/‑27) frdA ← / → epmA fumarate reductase flavoprotein subunit/EF‑P‑lysine lysyltransferase
RA 4,382,970 C→T P110S (CCT→TCT)  epmA → EF‑P‑lysine lysyltransferase
RA 4,391,611 C→T A3V (GCC→GTC)  orn → oligoribonuclease
RA 4,393,631 C→T R146H (CGC→CAC)  queG ← epoxyqueuosine reductase
RA 4,393,792 C→T T92T (ACG→ACA)  queG ← epoxyqueuosine reductase
RA 4,396,787 C→T T241T (ACC→ACT)  amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,399,104 C→T L565L (CTG→TTG)  mutL → DNA mismatch repair protein MutL
RA 4,405,238 C→T Y184Y (TAC→TAT)  purA → adenylosuccinate synthetase
RA 4,410,409 G→A E93K (GAG→AAG)  yjfI → DUF2170 domain‑containing protein YjfI
RA 4,413,615 G→A Q196Q (CAG→CAA)  yjfC → putative acid‑‑amine ligase YjfC
RA 4,435,880 G→A S229F (TCT→TTT)  cpdB ← 2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase/3'‑nucleotidase
RA 4,438,359 G→A G218D (GGC→GAC)  ytfI → protein YtfI
RA 4,438,403 G→A G233S (GGT→AGT)  ytfI → protein YtfI
RA 4,444,962 G→A S284N (AGC→AAC)  tamB → translocation and assembly module subunit TamB
RA 4,447,525 G→A M1138I (ATG→ATA)  tamB → translocation and assembly module subunit TamB
RA 4,449,507 G→A A49V (GCG→GTG)  ppa ← inorganic pyrophosphatase
RA 4,461,722 G→A N313N (AAC→AAT)  nrdD ← anaerobic ribonucleoside‑triphosphate reductase
RA 4,462,867 G→A intergenic (‑207/+187) nrdD ← / ← treC anaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑phosphate hydrolase
RA 4,477,303 G→A intergenic (+52/+4) yjgL → / ← argI protein YjgL/ornithine carbamoyltransferase ArgI
RA 4,479,109 G→A G143G (GGC→GGT)  yjgM ← putative N‑acetyltransferase YjgM
RA 4,479,694 G→A intergenic (‑157/‑36) yjgM ← / → yjgN putative N‑acetyltransferase YjgM/inner membrane protein YjgN
JC JC 4,536,236 IS5 (+) +4 bp intergenic (‑2183/‑358) topAI ← / → ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
RA 4,539,264 C→T V80I (GTT→ATT)  nanC ← N‑acetylneuraminate outer membrane channel
RA 4,542,683 Δ1 bp intergenic (+50/‑432) fimE → / → fimA regulator for fimA/type 1 fimbriae major subunit
RA 4,545,733 G→A S213N (AGT→AAT)  fimD → type I fimbriae usher protein
RA 4,559,285 G→A T81M (ACG→ATG)  iadA ← isoaspartyl dipeptidase
RA 4,561,613 G→A L355F (CTC→TTC)  yjiJ ← putative transporter YjiJ
RA 4,571,292 G→A L95L (CTG→TTG)  yjiR ← fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
RA 4,572,651 G→A D80N (GAT→AAT)  yjiT → putative uncharacterized protein YjiT
RA 4,577,641 G→A N106N (AAC→AAT)  mcrC ← Type IV methyl‑directed restriction enzyme EcoKMcrBC subunit
RA 4,600,636 G→A H114Y (CAT→TAT)  dnaC ← DNA replication protein DnaC
RA 4,610,221 G→A D270N (GAT→AAT)  prfC → peptide chain release factor RF3
RA 4,613,753 G→A K91K (AAG→AAA)  yjjV → putative metal‑dependent hydrolase YjjV

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196304 1211472 15169 36 [34] [35] 38 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270–1978503 9–776 139 [0] [0] 122 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423865–3424234 3424493–3424239 6–629 36 [35] [35] 36 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)56 (0.550) 41/598 1.4 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 382183 NA (NA)noncoding (924/1331 nt) IS2 repeat region
* ? NC_000913 382179 =NA (NA)34 (0.340) 29/594 2.6 NA noncoding (920/1331 nt) IS2 repeat region
?NC_000913 = 382590 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 12994980 (0.000)127 (1.270) 76/590 0.1 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)138 (1.360) 92/598 0.0 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)122 (1.200) 78/598 0.1 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL