Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,220,489	C→T	T332T (ACC→ACT)	patA →	putrescine aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,220,489	0	C	T	100.0%	188.3 / NA	53	T332T (ACC→ACT)	patA	putrescine aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/24);  total (29/24)

GATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATT  >  NC_000913/3220210‑3220774                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                              gcttCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGACACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGc                                                                                                                                                                                                                                                                           >  2:515012/3‑300 (MQ=255) gATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGc                                                                                                                                                                                                                                                                           >  2:524396/1‑300 (MQ=255)  aTTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGcc                                                                                                                                                                                                                                                                          <  1:677352/300‑1 (MQ=255)  aTTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCNTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCNTACCACCACTTTTGGCGGCAACCCGCTGGcc                                                                                                                                                                                                                                                                          <  2:572011/300‑1 (MQ=255)       gggTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGAATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCAATTTTGGCGGCAACCCGCTGGCCTGTgc                                                                                                                                                                                                                                                                     >  2:234769/1‑300 (MQ=255)            aaGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTgcggcgg                                                                                                                                                                                                                                                                <  2:736067/300‑1 (MQ=255)             aGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGNTGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTgcggcggc                                                                                                                                                                                                                                                               >  1:567160/1‑300 (MQ=255)                            gccgccgccGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCaa                                                                                                                                                                                                                                                >  2:480735/1‑300 (MQ=255)                                     gggCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCt                                                                                                                                                                                                                                       >  1:463154/1‑300 (MQ=255)                                      ggCTATCTCACCTCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCACACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCTGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACTATCAATGTGTTGCTg                                                                                                                                                                                                                                      <  2:64771/300‑1 (MQ=255)                                       gCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCNCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTNTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTgg                                                                                                                                                                                                                                     <  2:770863/300‑1 (MQ=255)                                               aCCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAAc                                                                                                                                                                                                                             <  2:119847/300‑1 (MQ=255)                                                    cgtacgtaAGCTATGCGATGAGTTAGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTAcc                                                                                                                                                                                                                        <  1:779875/300‑1 (MQ=255)                                                     gtacgtaAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCAACACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCg                                                                                                                                                                                                                       >  2:285653/1‑300 (MQ=255)                                                             gCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTTAATAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGc                                                                                                                                                                                                               <  2:36741/300‑1 (MQ=255)                                                                   cggtGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATATTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCGCTGCCTTGCCAAAGCGCTCGGCGGCGGNGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCa                                                                                                                                                                                                         <  2:206487/297‑1 (MQ=255)                                                                       gAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCaaaaa                                                                                                                                                                                                     <  1:650059/300‑1 (MQ=255)                                                                              gcgcACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGACAAGATGTTCGCCTGCGAGCATGAGACCGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGCGCAGAACTTACCGGCTCAGGTTGAGCAAAAAGGCgcta                                                                                                                                                                                              >  2:381864/1‑297 (MQ=255)                                                                                    tgatgaTCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGc                                                                                                                                                                                        <  1:184464/300‑1 (MQ=255)                                                                                     gatgatCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGAACATCGCCAATGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCt                                                                                                                                                                                       >  1:109319/1‑300 (MQ=255)                                                                                      atgatCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTg                                                                                                                                                                                      <  1:259634/300‑1 (MQ=255)                                                                                         atCCTCGATGAAGTACAAACGGGCATTGTGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGAc                                                                                                                                                                                   <  2:156322/300‑1 (MQ=255)                                                                                                     gTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCaa                                                                                                                                                                       >  1:746977/1‑300 (MQ=255)                                                                                                     gTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTATGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCAACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCaa                                                                                                                                                                       >  2:525245/1‑300 (MQ=255)                                                                                                          aaCGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGgc                                                                                                                                                                  >  2:776390/1‑300 (MQ=255)                                                                                                                      gcgcACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGNGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGNATATcc                                                                                                                                                      >  2:670053/1‑300 (MQ=255)                                                                                                                      gcgcACCGGCAATATGTTCGCCTGCGAGCATGAGAACATACAGCCGGATATCTTCTGCCTTGCCAAAGCGCTCGGCGTCTGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTNTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGTCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGTCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATcc                                                                                                                                                      <  2:184723/300‑1 (MQ=255)                                                                                                                             ggCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTg                                                                                                                                               >  2:742139/1‑300 (MQ=255)                                                                                                                             ggCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTg                                                                                                                                               >  1:721066/1‑300 (MQ=255)                                                                                                                                     gTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGa                                                                                                                                       <  1:443650/300‑1 (MQ=255)                                                                                                                                                      agaACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGa                                                                                                                      <  1:791634/300‑1 (MQ=255)                                                                                                                                                      agaACATACAGCCGGATATCCTCTGCCTTGCTAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTTACGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTNTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACTATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGa                                                                                                                      <  2:210595/300‑1 (MQ=255)                                                                                                                                                           gTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGACACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTg                                                                                                                 >  1:431896/1‑300 (MQ=255)                                                                                                                                                                  cGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCNGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGa                                                                                                          >  1:585891/1‑300 (MQ=255)                                                                                                                                                                        tCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGt                                                                                                    >  2:468073/1‑300 (MQ=255)                                                                                                                                                                        tCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCAGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGt                                                                                                    >  2:64797/1‑300 (MQ=255)                                                                                                                                                                        tCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCAATTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCAGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGt                                                                                                    >  1:276859/1‑300 (MQ=255)                                                                                                                                                                         ccTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGtt                                                                                                   >  1:85929/1‑300 (MQ=255)                                                                                                                                                                                                      gcgTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTg                                                                      >  1:516585/1‑300 (MQ=255)                                                                                                                                                                                                        gTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGcc                                                                    <  1:650727/300‑1 (MQ=255)                                                                                                                                                                                                                        cgACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCNTACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGcc                                                    <  2:620069/300‑1 (MQ=255)                                                                                                                                                                                                                        cgACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGcc                                                    <  2:126455/300‑1 (MQ=255)                                                                                                                                                                                                                                 gtataagagacaggtTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTAc                                           <  1:682552/287‑1 (MQ=255)                                                                                                                                                                                                                                 ccACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTAc                                           >  2:168880/1‑300 (MQ=255)                                                                                                                                                                                                                                 ccACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTGGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTAc                                           <  1:295887/300‑1 (MQ=255)                                                                                                                                                                                                                                              gtTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTNAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTNGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTGTAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTAcctgtctcttatac                              >  2:667141/1‑287 (MQ=255)                                                                                                                                                                                                                                              gtTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAAc                              <  1:829601/300‑1 (MQ=255)                                                                                                                                                                                                                                                tttCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGANCAAAAAGGCGATATGTTGCTGGACGGNTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTCCTGGTGGCAGGAACGc                            >  2:546474/1‑300 (MQ=255)                                                                                                                                                                                                                                                tttCAGTTCTGTTCGACAACCCATTCCTGCATACCACCAATTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCAGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGc                            >  1:448772/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  aCCCATTCCTGCATACCACCACTTTTGGCGGNAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTNCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGCGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAGCGa          >  2:590794/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       ttCCTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGc     >  2:471339/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         ccTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCAt   <  1:821079/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          cTGCATACCACCACTTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCAtt  >  1:62267/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                              GATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATT  >  NC_000913/3220210‑3220774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑