| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,232,319 | C→T | L219F (CTC→TTC) | fadH → | 2,4‑dienoyl‑CoA reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,232,319 | 0 | C | T | 98.5% | 235.6 / ‑6.4 | 67 | L219F (CTC→TTC) | fadH | 2,4‑dienoyl‑CoA reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (36/30); minor base G (0/1); total (36/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGCTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCT > NC_000913/3232027‑3232612 | ggCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATTAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGATGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCt < 2:314121/300‑1 (MQ=255)ggCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCt > 1:65852/1‑300 (MQ=255) cccATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCAATGTTCGACTTGGTc > 2:246233/1‑300 (MQ=255) aaCCGTTTCGTGCCCCATTAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGAc < 1:639214/300‑1 (MQ=255) ccccATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACtttt > 1:816048/1‑300 (MQ=255) gAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGaa > 2:51490/1‑300 (MQ=255) gAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATNTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGaa > 1:579430/1‑300 (MQ=255) gAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATNTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGNGCTTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTAACGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGaa > 2:663835/1‑300 (MQ=255) aaGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGt > 1:623674/1‑300 (MQ=255) gCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACNCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGNCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTag > 2:651332/1‑300 (MQ=255) aTGAAGAGATCCTGCAACTGATCGACAATTTNGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTNCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGAACTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGc > 2:581183/1‑300 (MQ=255) aaGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTgg > 1:783955/1‑300 (MQ=255) tCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGCCCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAgg > 2:692089/1‑300 (MQ=255) tCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCaag > 2:253484/1‑298 (MQ=255) tCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAAACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGAGCAgg > 2:237150/1‑300 (MQ=255) tCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGNGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAgg > 1:560301/1‑300 (MQ=255) aaCTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGTAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTg < 2:799856/300‑1 (MQ=255) gcgcAACTGGCGCGGGATGCAGGATACGACGGTGTAGAGTTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAac < 1:67266/300‑1 (MQ=255) gggAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTANCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATtggct < 1:611997/300‑1 (MQ=255) gCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCAt < 1:817882/300‑1 (MQ=255) ggATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTNGAACTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGaa > 2:729471/1‑300 (MQ=255) gtgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCNCCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAANCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTc > 2:762673/1‑300 (MQ=255) gtgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTc > 1:209647/1‑300 (MQ=255) gtgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACAACGGCATTGGCTGGCATGAAGCACGTATTc > 2:149244/1‑300 (MQ=255) gtgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGACGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTc < 1:192341/300‑1 (MQ=255) tGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATNATTATCTACCGACTGTCGATGTTCGANCTGGTCGAAGACGGCGGGACTTTTTCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCa < 2:564771/300‑1 (MQ=255) gggTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGTGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTCTCTACCGACTGTCGATGTTCGAACTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAAAACCGGCATTGGCTGGCATGAAGCCCGTATTCCGACCATTGCCAc > 2:140265/1‑300 (MQ=255) ttCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGcc < 2:218796/300‑1 (MQ=255) gAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGc < 1:22707/300‑1 (MQ=255) ttCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTggg > 2:512909/1‑300 (MQ=255) tCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCTGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGTTCGAAGACGGCGGGACTTTTGCCTAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGt < 2:264879/300‑1 (MQ=255) tCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGt < 1:279382/300‑1 (MQ=255) gcgcACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCaa > 1:338266/1‑300 (MQ=255) gcACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTNCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTNCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAAc > 2:770538/1‑300 (MQ=255) ccAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGAACTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGa > 1:637995/1‑300 (MQ=255) aaTCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGaaa > 2:323167/1‑300 (MQ=255) cAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGc < 1:720619/300‑1 (MQ=255) gCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAATGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGACGCACGTATTTCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCa > 2:343182/1‑300 (MQ=255) gCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTCTCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGCGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCAACGCCCGTGCAGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCa > 2:214929/1‑300 (MQ=255) gTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGNAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACg > 2:203927/1‑300 (MQ=255) cggcgATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGt < 1:408293/300‑1 (MQ=255) cgATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAac > 1:654892/1‑300 (MQ=255) ttACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAaccac < 1:529404/300‑1 (MQ=255) ttACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTAACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAaccac < 1:391132/300‑1 (MQ=255) gCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTACGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAAcc < 2:31518/300‑1 (MQ=255) cAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCg < 1:823076/300‑1 (MQ=255) aTGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAAc < 1:21852/300‑1 (MQ=255) gCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGATCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGa < 2:350636/300‑1 (MQ=255) gCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGa < 1:308620/300‑1 (MQ=255) gCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAg > 1:780890/1‑300 (MQ=255) ccGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGTTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAgg < 1:363661/300‑1 (MQ=255) tAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTg > 1:18573/1‑300 (MQ=255) gAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGTGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTAGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGcc > 2:163441/1‑300 (MQ=255) gAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCACCACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGNCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCANGTTGca > 2:688620/1‑299 (MQ=255) gtgcggtgcgCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATtc < 2:221254/300‑1 (MQ=255) atgtgtataagagacagggCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATtctct < 1:300212/283‑1 (MQ=255) gtgcgCGAACGCGTCGGCAACGACTTCATTATTATCTAACGACTGTCGATGTTCGACCTGGTCGCAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTAGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGAAGGTTGCCGACGATATTCTATcg > 2:511503/1‑300 (MQ=255) tCGGCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCg < 2:697794/300‑1 (MQ=255) ggCAACGACTTCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATtctctctgtctattatacacat > 2:297596/1‑284 (MQ=255) cAACGACTTCATGAGTATCTACCGACTTTCGATGTTTGACCTGGTCGACGACGGCGGGACTTTTGCCGAAACGTGAGAGCTGGCGCATGCCATTGAATCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTACCACACCCGTGCCACGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCAatat < 2:257144/300‑1 (MQ=255) aCTTCATTATTATCTACCTACTGTCGATGTTCGACCTGGTCGAAGACGGCTGGACTTTTGCCTAAACGTTAGAGCTGGCGCAGGCCATTGAAGCGGCGTGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTTTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTAt < 2:773297/300‑1 (MQ=255) aCTTCATTATTATCTACCGACTGTCGATGTTCTACCTGGTCGGAGATGGCGGGACTTTTGCCTGAACGGTAGAGCTGGCGCAGGCCATTTAATTGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTTCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAGGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCTACGATATTCTCTCGCGTGGCGATGCCGATATGGTAt < 2:400777/300‑1 (MQ=255) tCATTATTATCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGNTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGNCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGa > 2:649698/1‑300 (MQ=255) atCTACCGACTGTCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGa > 1:627411/1‑300 (MQ=255) accaaCTGTCGATGTTCGACCTAGGCGAAGACGGCGGGACTTTTGCCGAAACATTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAATGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACAATCCGCAGGCTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGt < 2:16994/296‑1 (MQ=255) tCGATGTTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCt < 1:515418/300‑1 (MQ=255) tCGATGGTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGGAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCt < 2:704363/300‑1 (MQ=255) | GGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGCTCGACCTGGTCGAAGACGGCGGGACTTTTGCCGAAACGGTAGAGCTGGCGCAGGCCATTGAAGCGGCGGGCGCGACCATTATCAACACCGGCATTGGCTGGCATGAAGCACGTATTCCGACCATTGCCACGCCCGTGCCGCGCGGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCT > NC_000913/3232027‑3232612 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |