breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 78 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,326,4500TG36.0% 202.7 / 20.3 100G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_0009131,435,7150GA34.0% 78.5 / 16.6 50intergenic (‑96/+45)uspF/ompNuniversal stress protein F/outer membrane porin N
*NC_000913811,7210TG32.6% 227.9 / 27.9 141V67G (GTG→GGG) bioCmalonyl‑acyl carrier protein methyltransferase
*NC_0009131,435,7270GA32.1% 92.8 / 23.5 53intergenic (‑108/+33)uspF/ompNuniversal stress protein F/outer membrane porin N
*NC_0009131,435,7180GA31.4% 91.8 / 14.5 51intergenic (‑99/+42)uspF/ompNuniversal stress protein F/outer membrane porin N
*NC_0009131,435,7250CA31.4% 84.3 / 18.8 52intergenic (‑106/+35)uspF/ompNuniversal stress protein F/outer membrane porin N
*NC_0009132,810,7580GA31.2% 56.7 / 37.9 48intergenic (+79/‑12)ygaH/mprAL‑valine exporter, YgaH component/DNA‑binding transcriptional regulator MprA
*NC_0009131,825,0610GA31.1% 117.4 / 20.4 61intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
*NC_0009132,483,2680TA30.8% 58.8 / 10.6 39L24F (TTA→TTTemrKtripartite efflux pump membrane fusion protein EmrK
*NC_0009133,665,7020CA30.4% 75.5 / 11.7 48A37S (GCG→TCG) gadXDNA‑binding transcriptional dual regulator GadX
*NC_0009134,213,7650AC29.7% 142.3 / 10.2 75V120G (GTG→GGG) yjaBpeptidyl‑lysine N‑acetyltransferase YjaB
*NC_0009132,558,5310AC29.5% 245.1 / 11.1 114V50G (GTG→GGG) eutBethanolamine ammonia‑lyase subunit alpha
*NC_0009135,1590GA28.8% 102.8 / 13.4 54intergenic (+139/‑75)thrC/yaaXthreonine synthase/DUF2502 domain‑containing protein YaaX
*NC_0009131,435,7070CA28.6% 107.0 / 12.9 56intergenic (‑88/+53)uspF/ompNuniversal stress protein F/outer membrane porin N
*NC_0009134,622,2500AG28.6% 213.1 / 18.4 98E161G (GAG→GGG) yjjJtoxin YjjJ
*NC_0009133,261,0550AC28.4% 277.7 / 14.1 134V455G (GTG→GGG) tdcE2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4
*NC_0009131,825,0560GA28.3% 110.7 / 20.1 60intergenic (‑119/+84)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
*NC_0009131,104,1750TG28.2% 147.0 / 13.4 71G75G (GGT→GGGcsgBcurlin, minor subunit
*NC_0009132,434,8820TG28.1% 188.9 / 15.9 91Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
*NC_0009133,665,6980TC28.0% 93.3 / 13.8 52D38G (GAT→GGT) gadXDNA‑binding transcriptional dual regulator GadX

Marginal new junction evidence (lowest skew 10 of 11 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1411899 =83 (0.770)7 (0.070) 7/444 5.8 9.8% coding (50/936 nt) ttcA tRNA cytosine(32) 2‑sulfurtransferase TtcA
?NC_000913 = 1434958 55 (0.570)intergenic (‑552/+227) ynaM/uspF protein YnaM/universal stress protein F
* ? NC_000913 = 2346779149 (1.380)3 (0.030) 3/482 7.9 2.5% coding (1915/2286 nt) nrdA ribonucleoside‑diphosphate reductase 1 subunit alpha
?NC_000913 3421147 = 90 (0.860)coding (1014/1182 nt) yhdX putative ABC transporter membrane subunit YhdX
* ? NC_000913 = 11417294 (0.870)3 (0.030) 3/492 7.9 2.4% coding (729/1044 nt) guaC GMP reductase
?NC_000913 858383 = 153 (1.430)coding (679/1266 nt) ybiU DUF1479 domain‑containing protein YbiU
* ? NC_000913 3570760 =163 (1.510)3 (0.030) 3/496 8.0 1.9% coding (560/1974 nt) glgX limit dextrin alpha‑1,6‑glucohydrolase
?NC_000913 = 4186474 145 (1.340)coding (1125/4224 nt) rpoC RNA polymerase subunit beta'
* ? NC_000913 2936345 =130 (1.200)3 (0.030) 3/496 8.0 2.3% coding (762/1776 nt) fucI L‑fucose isomerase
?NC_000913 = 4024217 124 (1.150)coding (664/783 nt) tatD 3' ‑> 5' ssDNA/RNA exonuclease TatD
* ? NC_000913 = 1799821114 (1.050)3 (0.030) 3/496 8.0 2.6% coding (179/198 nt) rpmI 50S ribosomal subunit protein L35
?NC_000913 = 2094829 113 (1.040)coding (295/591 nt) hisH imidazole glycerol phosphate synthase subunit HisH
* ? NC_000913 1767419 =133 (1.230)3 (0.030) 3/496 8.0 2.5% coding (1267/3057 nt) ydiJ putative FAD‑linked oxidoreductase YdiJ
?NC_000913 = 2546108 104 (0.960)coding (336/897 nt) murQ N‑acetylmuramic acid 6‑phosphate etherase
* ? NC_000913 = 153141861 (0.560)3 (0.030) 3/496 8.0 4.7% intergenic (‑914/‑398) yncO/ydcC protein YncO/H repeat‑associated putative transposase YdcC
?NC_000913 1531422 = 61 (0.560)intergenic (‑918/‑394) yncO/ydcC protein YncO/H repeat‑associated putative transposase YdcC
* ? NC_000913 1455109 =132 (1.220)3 (0.030) 3/496 8.0 2.0% coding (242/288 nt) paaB phenylacetyl‑CoA 1,2‑epoxidase subunit B
?NC_000913 4019841 = 166 (1.530)coding (218/606 nt) ubiJ ubiquinone biosynthesis accessory factor UbiJ
* ? NC_000913 = 495833120 (1.110)3 (0.030)
+G
3/494 8.0 2.9% coding (714/1875 nt) htpG chaperone protein HtpG
?NC_000913 2359549 = 85 (0.790)coding (604/1290 nt) yfaV putative transporter YfaV