Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,261,0550AC28.4% 277.7 / 14.1 134V455G (GTG→GGG) tdcE2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4
Reads supporting (aligned to +/- strand):  ref base A (80/16);  new base C (3/35);  total (83/51)
Fisher's exact test for biased strand distribution p-value = 1.74e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 135 total aligned reads displayed.

GTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGG  >  NC_000913/3260806‑3261305
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                           
gTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCAc                                                                                                                                                                                                                                                            >  2:113842/1‑251 (MQ=255)
    ccACCGACAGGCCCGCGCTGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCccg                                                                                                                                                                                                                                                        <  2:311257/251‑1 (MQ=255)
     cACCGACAGGCACGCGATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGACATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGACAGTCCATGAAG‑TGATCGAGGCAGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCACCAGCGGTGCTGTTTTCGACCCGACCTGGATCTTC‑AGCTTC‑TCGTCCACCccgc                                                                                                                                                                                                                                                       >  2:204425/1‑251 (MQ=39)
             gtcccGCGATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAAtt                                                                                                                                                                                                                                               >  2:691876/3‑251 (MQ=255)
               cccgggatcccgcagccattagggCGATAGCCATCACGATCGTGCACCCCCATCAGCGAGGCTTCGTAGTTGTCCTTG‑TGG‑TGCATGTAGTGAATGATATCCAGCGGGTTGATGTACTGCCCCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCCCTTTG‑TGGTAACCCAGCCCGTCGCCCATCAGCGGTGCGGTTTTCGGCCCGACCGGAATCTTC‑AGCTTC‑TCGCCCCCCCCGCCGTTAATTGc                                                                                                                                                                                                                                             <  1:460932/228‑1 (MQ=255)
                 cgcgATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGt                                                                                                                                                                                                                                           >  1:402891/1‑251 (MQ=255)
                 cgcgATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGt                                                                                                                                                                                                                                           >  2:140113/1‑251 (MQ=255)
                 cgcgATGCCGCATGCCATAGGGCGATAGACACCACGATCGTGCAGCGCCATCAGCGAGGCTTCGTGGTTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGTTGTACTGCCCCGCCAGCCAGTCCATGAAG‑TGATCGGGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGCCCATCAGCGGTGCTGTTTCCGGCCCGCCCTGATTCTTC‑AGCTTC‑TCGTCCCCCCCGCCGTTAATTGCGt                                                                                                                                                                                                                                           <  1:100046/251‑1 (MQ=255)
                     aTGCCGAATCCCATAGTGCGAAAGACATCCCGTTCGTGCACCCCCATCAGCGAAGCTTCGTAGCTGTCTTTG‑TCG‑TGCATGTAGTGAATGATATCCGGCGCGCTGATGTCCTGCCCCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCCCTTTG‑TCGTAACCCAGCCCGTCGCCCACCGGCGGTGCTGTTTCCGGCCCGCCCTGAATCTTC‑AGCTTC‑TCGCCCCCCCCGCCGTTAATTGCGTagag                                                                                                                                                                                                                                       <  1:619143/251‑1 (MQ=255)
                       gCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGagc                                                                                                                                                                                                                                      >  1:160869/1‑250 (MQ=255)
                        cagCATGCCATCGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGACGATTCGTAGCTGCACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCAATGAAG‑TCATCGCGGCTGTCCATCCCTTTG‑TCGTAAACCAGCACGTCGTCCATCACCGGTGCTGATTTCGACCCGACCTGAACCTTC‑ACCTTC‑TCGTCCCCCCCGCCGTTAACTGCGTAGagc                                                                                                                                                                                                                                      >  2:258391/3‑249 (MQ=255)
                           cATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGt                                                                                                                                                                                                                                 >  2:281665/1‑251 (MQ=255)
                              gCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGtttt                                                                                                                                                                                                                              >  1:168135/1‑251 (MQ=255)
                                       ggtaagccttCACGATCGTGCAGCGCCAGCAGCGAATCTTCGTGGTTGT‑CTTGGTCT‑GGCATGTAGTGAATGTTATTCAGCGGGCTGTTTTACTGCCCCGCCAGCCAGCCCATGAAG‑TGATCGAGGCTGTCCATCTTTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTCCTGTTTTCGCCCGGACCTGAATCTTC‑ATCTTC‑TCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGtt                                                                                                                                                                                                                     <  1:532854/242‑1 (MQ=255)
                                       cGATAGACATCACGATCGGGCAGCGCCATCAGCGAAGCTTGTTAGCTTTTTTTG‑TCG‑TGCATGTAGTGAATAATATTCGGCGCGCTGATGTACTGCCCCGCCAGCCAGTCCAGGAAG‑TGATCGAGGCTGTCCATCTCTTTG‑TCGTAACCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGtt                                                                                                                                                                                                                     <  1:647662/251‑1 (MQ=255)
                                        attataCATCACGTTCGTGCACCGCCGTCGGCGAAGTTTCTTGGCTGTATTTG‑TCGGTG‑ATGGAGTGATTTATTTTCAGCGTGCTGATGTACTCCTCCGCCAGCCATTCCATGAAG‑TAATCGAGGCTGTCATTCATTTTG‑TTATAACCCAGCTCGTCGTCCATCAGCGTTGCTGTTTCCGGCCCGACCTGAGTCTTC‑AGCTTC‑TCTTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTa                                                                                                                                                                                                                    <  1:591180/246‑1 (MQ=255)
                                           aGACACCAGGATCGTGCAGCGCCATCAGGGAAGTTTCGTGGCTGTCTTTG‑TGG‑TGCATTTGGTAAATGATATTCAGGGCTCTGTTTTATGGCACGCCCGGCCAGCCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGGGGTGTTGTTTTCGGCCCGCCCTGAATCTTC‑AGCTTC‑TCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgcg                                                                                                                                                                                                                 <  1:334337/251‑1 (MQ=255)
                                                 cACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGc                                                                                                                                                                                                            >  1:204025/1‑250 (MQ=255)
                                                  aCGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGCGCAc                                                                                                                                                                                                          >  2:651657/1‑251 (MQ=255)
                                                   cGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAc‑c                                                                                                                                                                                                        >  1:209059/1‑251 (MQ=255)
                                                      tCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑Cac                                                                                                                                                                                                      >  1:125235/1‑249 (MQ=255)
                                                        gTGCAGCGCCATCAGCGAAGCCTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑Caa                                                                                                                                                                                                      >  2:159512/1‑248 (MQ=255)
                                                            aGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGCATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTg                                                                                                                                                                                               >  2:281835/1‑251 (MQ=255)
                                                              cgcCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTCGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGc                                                                                                                                                                                              >  2:483588/1‑250 (MQ=255)
                                                               gcCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑CGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCCGGTTAGCGCGTGCAC‑CAACGAACTGCAt                                                                                                                                                                                            >  2:679369/1‑251 (MQ=255)
                                                                       cgagtcTTCGTAGCTGT‑CTTGGTCG‑TGCATGTATAGAATGTTATTCGGCGCGCTGTTTTCCCCCCCCGCCAGCCAGCCCAGGAGG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCAACCAGCGGTCCTGTTTTCGCCCCGCCCTGAATCTTC‑AGCTTC‑TCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTAc                                                                                                                                                                                    <  1:551700/246‑1 (MQ=255)
                                                                        gAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTAcc                                                                                                                                                                                   >  1:239188/1‑251 (MQ=255)
                                                                          agtttCGTAGTTGTATTTT‑TCT‑TGCATTTAGTGATTGATATTCAGCGCGCTAATTTCCTGCCCCGCCACCCAGTCCATGAAG‑GTATGGAGGCTTCCCCTCACTTTG‑CCGTAATCCAGCCCGTCGCCCATCGGGGGTGTTTTTTTCGGCCCGACCTGAATCTTC‑AGTTCC‑TCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCaa                                                                                                                                                                                 <  1:555331/248‑1 (MQ=255)
                                                                              tCGTAGCTGTATTTG‑TCG‑TGAATGTAGTGAATGATATCCAGCGCGCTGATTTACTGCCCCGCCGGCCAGTCCATGAAG‑TGATCGAGGCTGCCCATCTCTTTG‑TCGTACTCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCa                                                                                                                                                                              <  1:588727/250‑1 (MQ=255)
                                                                                       gttattgtcggggcttgtagaaaaTGATTTCCAGCGCGCTGATTTACTGCCCCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTTCCCATCTCTTTG‑TCGTAACCCAGCACGTCGTCCACCAGCGGTGCTTTTTTCGCCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTggg                                                                                                                                                                      <  1:213821/229‑1 (MQ=255)
                                                                                        aCTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTg                                                                                                                                                                   >  1:170131/1‑251 (MQ=255)
                                                                                            gtgcG‑TGAATGTAGTAAATGATATTCGGCGCGCTGATGTACGGCCCCGCCAGCCAGCCCATGAAG‑TGATCGAGGCTTTCCATCACTTTG‑TCGTAACCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTCC‑AGCTTC‑TCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACg                                                                                                                                                                <  1:324518/248‑1 (MQ=255)
                                                                                               cG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACGGCCAGCCAGTCCCTGAAG‑TGAACGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCATCCATCAGCGGGGCTGATTTCGGCCAGACCTGACTCTTC‑AGCTTC‑CCGTCCACCCCGCCGTTAATTGCGCAGGGCATCGATTTCGCCAGGTTCGTGCGTTCACGCAAAGA‑CTGCATTTGCTTACCAATCACGATTGTGCTGaagcc                                                                                                                                                              >  2:564531/1‑246 (MQ=255)
                                                                                                 gtgattgaaGTGAAAGATTTCCAGCGGGCTGATGTACTGCCCCGCCAGCCAGTCCATGAAG‑TGAGCGAGGCTGTCCATCACTTTG‑TCGTAACCCAGCACGTCGCCCATCAGCGGTGCTTTTTGCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagc                                                                                                                                                            <  1:443301/243‑1 (MQ=255)
                                                                                                   gcttGTAGTGAATGATATTCGGCGCGTTGTTTTACGGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCCCTTTG‑TCGTAACCCAGCACGTCGTCCACCAGCGGTGCTGTTTGCGGCCCGCCCTGAATCTTC‑AGCTTC‑TCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGcagcag                                                                                                                                                          <  1:143041/248‑1 (MQ=255)
                                                                                                     atttAGTGAATGATATCCGGCGCGCTGTTTTACTGCCCCGCCAGCCAGCCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAACCCAGCCCGTCGTCCATCGGCGGTGCTGTTTTCGGCCCGCCCTGATTCTCC‑AGCTTC‑TCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGc                                                                                                                                                        <  1:426821/248‑1 (MQ=255)
                                                                                                     aTGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGc                                                                                                                                                        >  1:642986/1‑251 (MQ=255)
                                                                                                     aTGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGc                                                                                                                                                        >  1:863232/1‑251 (MQ=255)
                                                                                                        tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGCGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAAt                                                                                                                                                     >  1:151076/1‑251 (MQ=255)
                                                                                                         aGTGAATGATATTCAGCGCGCTGATGTACTGCCCCGCCAGCCAGCCCTTGAAG‑TGATCGGGGCTGTCCATCCCTTTG‑TCGTAATCCAGCCCGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTC‑AGCTTC‑TCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATc                                                                                                                                                    <  1:390188/251‑1 (MQ=255)
                                                                                                           tGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcgc                                                                                                                                                  >  1:715849/1‑251 (MQ=255)
                                                                                                                   atccgggccggtgatttccgcccccGCCAGCCAGTCCA‑GAAGGTGATCGAGGCTGTCC‑TCCCTTTGGTCGTAACCCAGCCCTTCGTCCGACGGCGGTGCTGTTTTCGCCCCGACCTAAATCTTC‑AGCTTC‑CCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgt                                                                                                                                          <  1:281835/228‑1 (MQ=255)
                                                                                                                   atTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgt                                                                                                                                          >  2:702729/1‑251 (MQ=255)
                                                                                                                      cAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGc                                                                                                                                       >  1:491516/1‑251 (MQ=255)
                                                                                                                      cAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGc                                                                                                                                       >  2:611577/1‑251 (MQ=255)
                                                                                                                       ggcgctgtgatttccccccccGCCAGCCCGTCCGTGAGG‑TGAGCGAGGCTGCCAATCTTTTTG‑TCGTAACCCATCACGCAGCCCATCCGGGGGCCTTTTTTCGCCCCGACCTGAATCTCC‑GGCTGC‑CCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCt                                                                                                                                      <  2:285282/232‑1 (MQ=255)
                                                                                                                       aGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGGCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGCATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGGTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCt                                                                                                                                      >  1:757542/1‑251 (MQ=255)
                                                                                                                        gcgcgcTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTg                                                                                                                                     >  1:582034/1‑251 (MQ=255)
                                                                                                                             cTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTg                                                                                                                                  >  2:55056/1‑249 (MQ=255)
                                                                                                                                 tGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTc                                                                                                                             >  1:51868/1‑250 (MQ=255)
                                                                                                                                       ccACCGCCAGCCAGCCCATGAAG‑TGATCGAGGCTGTCCATCCTTTTG‑TCGTAATCCAGCACTTCGCACATCGGGGGGGCTGTTTTGGGCCCGCCCTGAATCTTC‑AGTTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGc                                                                                                                      <  1:872739/250‑1 (MQ=255)
                                                                                                                                         ccccccagccagTCCATAAAG‑TGATGGGGGCTGTCCATC‑CTTTGGTCGAAATCCAGCACGTCGTCCACCAGGGGTGCTGTTTGCGGCCCGCCCTGAATTTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCAt                                                                                                                    <  1:308125/247‑1 (MQ=255)
                                                                                                                                           cGCCAGCCAGTCCATGAAG‑TGATCGGGGCTGTCCATCACTTTG‑CCGAAACCCAGCCCGTCGCCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCa                                                                                                                  <  1:354461/251‑1 (MQ=255)
                                                                                                                                           cGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGGCAGTACGCATcc                                                                                                                  >  2:432566/1‑250 (MQ=255)
                                                                                                                                             ccagccagTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc                                                                                                                   >  2:619446/1‑248 (MQ=255)
                                                                                                                                               agccataccATAAAG‑TGATCAAGACTGTCCTTCACTTTG‑CCGAAACCCAGCACGCCTTCCACCGGCGGTGCTTTTTTCGGCCCGACCTGAA‑CTTCCAGCGTC‑TCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATc                                                                                                              <  1:822222/244‑1 (MQ=255)
                                                                                                                                                 ccagTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGAATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGCAGTCAGTACGCATCAGATCGt                                                                                                            >  2:522394/1‑251 (MQ=255)
                                                                                                                                                      ccATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAttt                                                                                                       >  1:681491/1‑251 (MQ=255)
                                                                                                                                                                  tCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACATAGTCCCTCAGCGGTGCTGTTTTCGGCCCGACCTGACTCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGAAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCAACAGATCGTCATTTTCATACTGCa                                                                                             >  1:834022/1‑250 (MQ=255)
                                                                                                                                                                    gAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTC‑AGCTTC‑TCGTCCCCCCCGCCGTTACTTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAACCACCATTGGGCTGACGCAGCAGGCATTCGAGTAATCGTAGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTTATACTGCAAgg                                                                                          >  2:399759/1‑251 (MQ=255)
                                                                                                                                                                    gAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAgg                                                                                          >  1:451967/1‑251 (MQ=255)
                                                                                                                                                                       gCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAttt                                                                                                       <  1:288416/235‑1 (MQ=255)
                                                                                                                                                                       gCTGCCCATCCCTTTG‑TCGAAACCCAGCCCGTCGTCCATCAGCGGTGCTTTTTTCGGCCCGACCTGAATCTCC‑AGCTTC‑CCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAag                                                                                       <  1:691876/251‑1 (MQ=255)
                                                                                                                                                                         tGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAag                                                                                       >  2:371073/1‑249 (MQ=255)
                                                                                                                                                                          gTCCATCACTTTG‑TCGTAATCCAGCACGTCGTACATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAgg                                                                                    >  1:597145/1‑251 (MQ=255)
                                                                                                                                                                            ccATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGACCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGAGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTAAGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTg                                                                                  >  2:256292/1‑251 (MQ=255)
                                                                                                                                                                               ccccTTTG‑TCGTAAGCCAGCACGTCTTCCACCGGCGGTGTTTTTTTCGGCCCGACCTGAAACTCC‑ACCTTC‑TCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACg                                                                               <  1:324076/248‑1 (MQ=255)
                                                                                                                                                                                    ttG‑TCGTAATCCAGCACGTCGTCCATCAGCGGGGCGGTTTTCGCCCCGACTTGAATCTTC‑A‑CTTCCTCTCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGAt                                                                             <  1:1938/248‑1 (MQ=255)
                                                                                                                                                                                      tgtcgtaacccggcccttcttcCATCAGGGGCGCTGATTTCGGCCCTCTCGGATTCTTC‑GTCTTC‑TCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGa                                                                          <  2:784607/230‑1 (MQ=255)
                                                                                                                                                                                         cGTAATCCAGCACTTCGTCCACCAGCGGTGCTGTTTCCGGCCCGCCCTGAATCTTC‑AGCTCC‑TCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGacac                                                                       <  1:464520/250‑1 (MQ=255)
                                                                                                                                                                                          gtaccccaccccatcctccaccggtggTGTTGTTTCCGGCCCACCCTGAATCTCC‑ATCTTC‑TCTCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCt                                                                     <  1:441371/226‑1 (MQ=255)
                                                                                                                                                                                          gTAATCCAGAATGTCGTCCATCAGCGGGGCTGTTATCCGCCCGACCTGACTCTTC‑AGCTTC‑TCGTCCCCCCCGCCGTTAATCGCGCAGAGCAGCGATTTCGCCAGGTGAGAGCGTGCCC‑CACAGAACTGCATTTGCTTACCACTCACCATGGGGCTGACGCACCAGGCAATCGCGTACTAGCCACTGTTTAAGTCAGTACGCATCAGATGGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCt                                                                     >  2:673063/1‑251 (MQ=255)
                                                                                                                                                                                           tAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTg                                                                    >  1:308335/1‑251 (MQ=255)
                                                                                                                                                                                             aTCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTCGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACAccgtcg                                                                  >  2:800156/1‑247 (MQ=255)
                                                                                                                                                                                                cAGCCCGTCGTCCATCAGCGGTGCTTTTTTCGGCCCGACCGAAATCTTC‑AGCTTC‑TCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGAt                                                                                                               <  1:555763/203‑1 (MQ=255)
                                                                                                                                                                                                 aGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGc                                                              >  2:228949/1‑251 (MQ=255)
                                                                                                                                                                                                  gCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGc                                                              >  2:312341/1‑250 (MQ=255)
                                                                                                                                                                                                   cACGTCGTCCATCAGGGGTGCTGTTTTGGGCCCGCCCTGATTCTCC‑AGCTTC‑TCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCat                                                            <  1:702729/251‑1 (MQ=255)
                                                                                                                                                                                                     cgtcgtCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGCTCGACACCTGCGCGGCatat                                                          >  2:546353/1‑251 (MQ=255)
                                                                                                                                                                                                      gtcgtcCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATAtt                                                         >  1:614400/1‑251 (MQ=255)
                                                                                                                                                                                                          tcCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTAGGCTGACGCAGCAGGCAATCGCGTAATCGTCGATGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCACGGAAGAGGTGACGATCGACACCTGCGCGGCATAtttttt                                                     >  2:730915/1‑251 (MQ=255)
                                                                                                                                                                                                          cccagcaggggttcTGTTTCCGCCCCGTCCTAAATCTTC‑AGCTCC‑TCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATAtttttt                                                     <  1:731907/238‑1 (MQ=255)
                                                                                                                                                                                                           ccATCAGCGGGTTTGTTTTCGGCCCGCCCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTg                                                    <  1:793947/251‑1 (MQ=255)
                                                                                                                                                                                                                    gtgttGTTTTCGGCCCAACCTTAACCTTC‑AGCTTC‑CCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATc                                           <  1:651657/247‑1 (MQ=255)
                                                                                                                                                                                                                     ggctttttccgcccccccctaattCTTC‑AGCTTC‑CCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCCCCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATc                                           <  1:522394/227‑1 (MQ=255)
                                                                                                                                                                                                                        tGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGt                                        >  1:370635/1‑250 (MQ=255)
                                                                                                                                                                                                                             tCGGCCCGACCTAAATCTTC‑AGCTTC‑CCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAAttc                                   <  1:146177/250‑1 (MQ=255)
                                                                                                                                                                                                                              cGGCCCGCCCTTATTCTTC‑TGCTTC‑TCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAAttctt                                 <  1:281665/251‑1 (MQ=255)
                                                                                                                                                                                                                              cGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAAttctt                                 >  1:131305/1‑251 (MQ=255)
                                                                                                                                                                                                                               ggCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAAttcttc                                >  1:359615/1‑251 (MQ=255)
                                                                                                                                                                                                                                ccccGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTcc                               <  1:515678/250‑1 (MQ=255)
                                                                                                                                                                                                                                    caccggAATCTTC‑AGTTCC‑CCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGGACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGAcc                           <  2:168135/246‑1 (MQ=255)
                                                                                                                                                                                                                                     cgccctgtctctcccctttcccccccACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGAc                            <  2:138818/227‑1 (MQ=255)
                                                                                                                                                                                                                                        tGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGTCCaa                         >  2:496062/1‑249 (MQ=255)
                                                                                                                                                                                                                                            tCTTC‑AGCTTC‑TCCACCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAAt                    <  2:266751/250‑1 (MQ=255)
                                                                                                                                                                                                                                              ttC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGt                 >  1:349014/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  aGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTc                >  1:258813/1‑249 (MQ=255)
                                                                                                                                                                                                                                                       c‑tcGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCaggtt           >  2:477608/1‑249 (MQ=255)
                                                                                                                                                                                                                                                           gTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCaggttaggtt      >  1:67529/1‑251 (MQ=255)
                                                                                                                                                                                                                                                           gTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCaggttaggtt      >  1:843433/1‑251 (MQ=255)
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                           
GTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCGATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTG‑TCG‑TGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAG‑TGATCGAGGCTGTCCATCACTTTG‑TCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTC‑AGCTTC‑TCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAC‑CAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGG  >  NC_000913/3260806‑3261305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑