Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,435,715	0	G	A	34.0%	78.5 / 16.6	50	intergenic (‑96/+45)	uspF/ompN	universal stress protein F/outer membrane porin N
Reads supporting (aligned to +/- strand):  ref base G (28/5);  new base A (0/17);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 2.67e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAACACCTGCCGGGTTGTCTGCACCACC  >  NC_000913/1435466‑1435963                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                          aaCCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTTCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCTATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑GCAAAggagga                                                                                                                                                                                                                                                          <  2:530049/251‑1 (MQ=255)  aCCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTGCCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGgaa                                                                                                                                                                                                                                                         <  2:15484/251‑1 (MQ=255)          agGCAGAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGGGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ATACTTTTT‑GTGGAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGc                                                                                                                                                                                                                                                 <  1:733554/251‑1 (MQ=255)              aTAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGa                                                                                                                                                                                                                                              >  1:571207/1‑250 (MQ=255)                   agGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGc                                                                                                                                                                                                                                        >  1:196438/1‑251 (MQ=255)                    gggCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGcc                                                                                                                                                                                                                                       >  2:792347/1‑251 (MQ=255)                        aGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑Gcc                                                                                                                                                                                                                                  <  2:194876/251‑1 (MQ=255)                         gTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTGATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑Gcc                                                                                                                                                                                                                                  >  1:107346/1‑250 (MQ=255)                                  ttACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCgg                                                                                                                                                                                                                        >  2:498251/1‑251 (MQ=255)                                   tACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCggg                                                                                                                                                                                                                       >  2:771786/1‑251 (MQ=255)                                            gAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGAt                                                                                                                                                                                                              >  2:72415/1‑251 (MQ=255)                                             aaaTGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGAtt                                                                                                                                                                                                             >  2:178646/1‑251 (MQ=255)                                                  aaCCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGa                                                                                                                                                                                                         >  2:659691/1‑250 (MQ=255)                                                         gCATCATCAATCTTTGCCTCTTCCTCAACGTGGC‑TAATCACGCTTTGATTTAATTCGGAATCGAAAATATCGATAGG‑GAAAAGAATCGTTCTGTTCATAAACCCTCCTGTTTTAGTATCCGAATAAAGTGAAAC‑GCCAGATG‑ACATTTTTT‑GTGTAAGGACGGAGTTCACATTTTAAATTA‑AGA‑TAAAAGGAGGAAAAATAAGAAAAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGAt                                                                                                                                                                                                   <  1:74735/249‑1 (MQ=255)                                                         ccatcatCAATTTTTGCCTCTCCCTAAACG‑GGCATAACCGCGGGTGAATTTAATTCTAAAGCGAAAATATGAATAGG‑GACAAGAAGCGTTCTGTTAAAAAAACCTCCTGTTTTAGTACCCGAATAAAGTGTAAC‑GCCAGAGG‑ACATTTTTT‑GTGAAAGGAGGGAGTTCACATTTTTAATTT‑AGA‑TAAAAGGAGGAAAAAAAAGAAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGAt                                                                                                                                                                                                   <  1:684961/248‑1 (MQ=255)                                                                 aaTCTTGGCCTCTCCCTCACGGGGGC‑AAATCACGCGTTGAGTTAATTCTAAATCGAAAATATGGA‑AGGAGAAAAGAATCTTTCTGTAAAAAAACCCTCTTTTTTTAGTACCCGAAAAAAGTATAAC‑GCCAAATG‑ACTCTTTTT‑GTGTAATGACGAAGTCCATTTTTTTAATTA‑AGA‑TAAAGGGAGAAAAAAAAAAAAAAAAAACCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAcc                                                                                                                                                                                         <  2:490378/251‑1 (MQ=255)                                                                      ttcccTCTTCCTAACCTTGGC‑AAATCACGTGTTGATTTAATTTTGAACCGAAAATATCGATAGG‑GAAAAAAACCTTTCTTTTAAAAAACCCGCTTTTTTTAGTACCCGAAAAAATTGTAAC‑GCAAGAGA‑ACATTTTTT‑GTGAAATGACGGAGTTCAC‑TTTTTAATTTTAAA‑TAAAGGGAGAAAGAAAAAGCAAAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTaaa                                                                                                                                                                                     <  1:784893/247‑1 (MQ=255)                                                                      ttGCCTCTTCCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GCCAAAAATCGTTTTGTTAAAAAACCCTCTTGTTTAAGAATCCCAATAAAGAGTACC‑GCCAGAGG‑ACACTTTTT‑GGGTAATGAGGGAGTTACCATTTTAATTTT‑AGA‑TCAAAGGAGGAAAAATAAGCAGAAAAAGCCC‑GCAATAAAAGCGGGCAGGAGGATTTAGAACTGATAAACAAGCCCTaa                                                                                                                                                                                      >  1:212024/1‑249 (MQ=255)                                                                        cccttttcccaaaCGTGGC‑AAATCACGCGTTGAGTTAATGCTGAACGGGAAATATCGATAGG‑AACAAGATTCGTTTTGTTCATAAACCCTCTTTTTTAAGTATCCGAAAAAAGTGTAAC‑GCCAGATG‑ACATTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTA‑AGA‑TCAAAGGAGAAAGAAGAAGAAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGc                                                                                                                                                                                   <  1:638540/239‑1 (MQ=255)                                                                            cttctaaaccGTGGC‑TAATCCCGCGTTGAGTTAATTCTAAACCGAAAATTTCGATAGG‑GAAAAAAATCGTTTTGTTCAAAAAACCTCCTTTTTTAGTATCCGCATAAAGTGAAAC‑GCAAGATG‑ACTTTTTTT‑GTGAAATGACGGAGTTCACATTTTTATTTA‑AGA‑TAAAGGGAGAAAAAATAAGAAAAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGAc                                                                                                                                                                                <  1:178646/240‑1 (MQ=255)                                                                                         gC‑TAATCACGCGTTGATTTAATTCTAAATAGAAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAACCCTCTTGTTTTAGTACCCGCATAAAGTGTAAC‑GCCAGATG‑ACATTTTTT‑GTGTAATGACGGAGTTCAAATTTTTATTTA‑AAA‑AAAAAGGAGAAAGAAAAAGAAAAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTaga                                                                                                                                                                 <  1:359201/251‑1 (MQ=255)                                                                                             aatccggcgTTGAGTTAATTCAGAAGCGAAAATATCAATAGG‑AAAAAGAATCTTTCTTTTAATAAACCCCCCTTTTTTAGTATCCGAAAAAAGTGTACC‑CCCA‑ATGGACTTTTTTT‑GTGTAATGACGGATTTCAATTTTTTAATTT‑AGA‑AAAAAGGAGAAAAAATAAG‑AAAAAAAGCCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTagag                                                                                                                                                                <  1:807241/243‑1 (MQ=255)                                                                                             aaTCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCCTAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTag                                                                                                                                                                  >  1:686712/1‑247 (MQ=255)                                                                                                       tGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTACTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCA‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACACTATCATCGGTAGAGATGCCATTGGc                                                                                                                                                     >  2:649462/1‑250 (MQ=255)                                                                                                            tAATTATGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGAAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGTCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGAAGAGATGCCATTGGCAGCGt                                                                                                                                                >  1:471359/1‑250 (MQ=255)                                                                                                               ttCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGa                                                                                                                                             >  2:875104/1‑250 (MQ=255)                                                                                                                   gAATCGAAAATATCAAGAGG‑GAAAAGAATCGTTTTGTTCAAAAACCCTCTTGTTTAAGTTCCCGCAAAAAGTGAAAC‑GCCGGAGG‑ACTTTTTTT‑GTTAAATGCCGGGTTTCACTTTTTTAATTT‑AAA‑CAAAAGGAGAAAAAAAAAGAAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCt                                                                                                                                         <  1:771786/250‑1 (MQ=255)                                                                                                                             tatCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCAt                                                                                                                              >  2:380867/1‑251 (MQ=255)                                                                                                                                    tagagACAAGAATCGTTCTGTTCATAAAACCTCATGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGATCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATAGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAAc                                                                                                                         >  2:60333/5‑250 (MQ=255)                                                                                                                                      g‑gACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACGGATAAACCAGACCTAAAGCGACAATATCATCGATAGAGATGCCATTGGCAGCGTAGAAGATGTCATCTTCATCCAACAGGt                                                                                                                     >  2:42443/1‑251 (MQ=255)                                                                                                                                                     tttttaataaaccttctttttttAGTAACCACAAAAATTGAAAC‑GCCAGTTA‑ACATTTGTT‑GTGGAAGGACGGGTTTCATTTTTTAAATTA‑AGA‑TAAAAGGAGAAAAAAAAAGAAAAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTc                                                                                                        <  2:571207/231‑1 (MQ=255)                                                                                                                                                               acccccctttttaagtacccgaaaaaaGTGTAAC‑GCCAGATG‑ACTTTTTTT‑GTTAAATGAGGGATTTCACATTTTTAATTT‑AAA‑AAAAGGGAGAAAAAAAAAGCAAAAAAGGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTgg                                                                                             <  1:792347/227‑1 (MQ=255)                                                                                                                                                                ccctcctGTTTTATTTCCCGCA‑AAAGTTGAACCGCCAAATG‑ACCTTTTTT‑GTGTAATGACGGAGTCCA‑ATTTTTTATTT‑AAAATAAAGGGAGAAAAAAAAAGAAAAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGa                                                                                            <  1:807952/250‑1 (MQ=255)                                                                                                                                                                    cctGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGCTG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATg                                                                                        >  2:224287/1‑251 (MQ=255)                                                                                                                                                                         tttattacccgtaaaaaGTGTAAC‑GCCAGATT‑ATATTTTTA‑GTAAAAGGAGGGAGTTCATTTTTTAAATTA‑AAA‑GCAAAGGAGAAAGAATAAAAAAAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtttt                                                                                    <  2:90161/236‑1 (MQ=255)                                                                                                                                                                             gTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTg                                                                               >  2:424777/1‑251 (MQ=255)                                                                                                                                                                               acccgaaTAAAGTGAAAC‑GCAAGATG‑A‑ACTTTTTTGTGAAAGGACGGATTTAACATTTATAATTA‑AAA‑AAAAAGAAGAAAAAAAAAAAAAAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGa                                                                              <  2:196438/244‑1 (MQ=255)                                                                                                                                                                                 cagaataaatgtaaaC‑GCAAGTTG‑ACTCTTTTT‑GGATAATGTCGGATTTAACATTTTAAATTA‑AGA‑AAAAAGGAGGAAAAAAAAGAAAAAAACGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGaaa                                                                            <  2:881510/237‑1 (MQ=255)                                                                                                                                                                                   gCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAAt                                                                           >  1:277863/1‑249 (MQ=255)                                                                                                                                                                                     aTAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGt                                                                        >  1:670174/1‑250 (MQ=255)                                                                                                                                                                                                   ccAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAat                                                           >  1:51151/1‑250 (MQ=255)                                                                                                                                                                                                     aGATG‑ACACTTTTT‑GTGTAAAGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCTC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCCTCCAACAGGTTGATTTTATAGTCCACGTAGGTGGACATGTTTTTATTGAAATAGCAAGTCGCGCCAATATc                                                        >  2:200341/1‑251 (MQ=255)                                                                                                                                                                                                      agaaaacttttttt‑GTGAAATGAGGGAGTTCAAATTTTTATTTT‑AGA‑TAAAAGAAGAAAAAAAAAGAAAAAAAACCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATc                                                        <  2:670174/242‑1 (MQ=255)                                                                                                                                                                                                        tG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGc                                                     >  1:228397/1‑251 (MQ=255)                                                                                                                                                                                                         g‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAAc                                                                                                                         >  1:402033/1‑182 (MQ=255)                                                                                                                                                                                                          gacttttttt‑GTTTAAGGAGGTAGTTCACTTTTTTAATTT‑AGA‑AAAAAGGAGAAAAAATAAGAAAAAAACGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGc                                                     <  1:498251/245‑1 (MQ=255)                                                                                                                                                                                                                      tgtAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGGGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGAAGGTGGACATGTTTTTATTGAATTAGTAAGTCGCGCAAATATCGGCGTATTTAAcc                                           >  2:552321/1‑249 (MQ=255)                                                                                                                                                                                                                       gtAATGACGGATTTCAAATTTTTAATAT‑AGA‑ACAAAGGAGAAAAAATAAGCAAAAAAACCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGa                                        <  1:846198/251‑1 (MQ=255)                                                                                                                                                                                                                          aggagggagtcaacttttttAATTT‑AGA‑AAAAAGGAGAAAAAAAAAAAAAAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGTTCt                                     <  1:380867/236‑1 (MQ=255)                                                                                                                                                                                                                             aCGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCttt                                   >  1:461244/1‑250 (MQ=255)                                                                                                                                                                                                                                             tAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAACACCTGCCg                  >  2:583473/1‑251 (MQ=255)                                                                                                                                                                                                                                                      aacaaaaGGAGAAAGAAAAAGAAAAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAACACCTGCCGGGTTGTc           <  1:875104/247‑1 (MQ=255)                                                                                                                                                                                                                                                               aggaAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAACACCTGCCGGGTTGTCTGCaccacc  >  1:475759/1‑251 (MQ=255)                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                          AACCCAGAGAGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGC‑TAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGG‑GACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAAC‑GCCAGATG‑ACACTTTTT‑GTGTAATGACGGAGTTCACATTTTTAATTT‑AGA‑TCAAAGGAGGAAGAATAAGCAGAAAAAGCCC‑GCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTATTTAACCAGATCTTTATCATCAACACCTGCCGGGTTGTCTGCACCACC  >  NC_000913/1435466‑1435963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑