Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0560GA28.3% 110.7 / 20.1 60intergenic (‑119/+84)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base G (38/5);  new base A (0/17);  total (38/22)
Fisher's exact test for biased strand distribution p-value = 6.80e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTG‑CGCCAGCATGTTAGCTTTGCGCTGTTC  >  NC_000913/1824808‑1825298
                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    
cgATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTga‑gaagaga                                                                                                                                                                                                                                                     >  2:36686/1‑250 (MQ=255)
          aaTGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAAccg                                                                                                                                                                                                                                          >  1:143354/1‑251 (MQ=255)
             gCTGGCACGCCAGTAAAAAGCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATCCTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACGGTTTGA‑GAAGAGAAAAGAAAAccgcc                                                                                                                                                                                                                                        <  2:115335/250‑1 (MQ=255)
               tGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGAt                                                                                                                                                                                                                                     >  1:689331/1‑251 (MQ=255)
                              aaTCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACCACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAtt                                                                                                                                                                                                                      >  2:115980/1‑251 (MQ=255)
                              aaTCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGc                                                                                                                                                                                                                         >  1:476483/1‑248 (MQ=255)
                                 cACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TT‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCAGATCCTGTCCACCGCATTACt                                                                                                                                                                                                                   >  2:65938/1‑251 (MQ=255)
                                  ccGTTTTGCGGGTGAGACCGTCAAAATTCCGCGTGTTCCCCCGGCA‑CATTTCCGCCACAGATCCCCCGACATTTTAC‑CATAATAAAAGTATTCCATACGCGCCCCCGGCCCTTTTTTTTTATTTTT‑ACCGTTCAGGTACCTGTGGATGGTTATCGGCAACTGGGG‑TA‑ATTT‑ACGTT‑A‑GTGGTGAT‑CGGGTTTTTTTTCCAACTTTTTGA‑GAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                                                                                                                  <  2:40509/250‑1 (MQ=255)
                                    gTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAGCTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                                                                                                                 >  2:269154/1‑250 (MQ=255)
                                    gTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGGTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCAACCGCATTACTGc                                                                                                                                                                                                                 >  2:884190/1‑250 (MQ=255)
                                             gTGGAAACGTGAAAATTTCGCGCGTTCCCCGGGCAGCATTCC‑GCCACAGATCCACCGACATTTTACGCATA‑CAAAAGTTTTCCATCCCCGCCCCCGGCCCTTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGGGGATGGTTATCGGCAACTGGGG‑TG‑TTTT‑ACGTT‑A‑GTGGTGAC‑CAGGTTTTTTTTACACCTGTTTGA‑AAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                                                                                                                       <  1:36686/251‑1 (MQ=255)
                                                 gAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                                                                                                                    >  2:646041/1‑250 (MQ=255)
                                                  aaCGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                                                                                                                    >  1:611751/1‑249 (MQ=255)
                                                   aCGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                                                                                                                    >  1:329352/1‑248 (MQ=255)
                                                      tGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcg                                                                                                                                                                                              >  1:329444/1‑251 (MQ=255)
                                                             ttCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATC‑TCAAAGTATTCCATCCTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGCTGGTTATCGGCAACTCGGG‑TG‑AGTT‑ACGTT‑C‑GTGGTGAT‑CAGGCTTTTTTTACAAATGTTTGA‑GTAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGtgt                                                                                                                                                                                       >  2:145741/1‑249 (MQ=255)
                                                             ttCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTAACCGACATTTTACGGATA‑TCAAAGTATTCCATACTCCCTCACGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑CTTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACCGTTTGA‑GAAGAGAAAAGAAAACCGCCGCTCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGTCGGTcc                                                                                                                                                                                       >  1:311726/1‑250 (MQ=255)
                                                               cgcgcgTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACACGACCGGCGGTCttt                                                                                                                                                                                     >  2:302064/1‑250 (MQ=255)
                                                               cgcgcgTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcggctttt                                                                                                                                                                                     >  2:765167/1‑246 (MQ=255)
                                                                               agcaTTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGaa                                                                                                                                                                     >  1:725308/1‑250 (MQ=255)
                                                                                     tC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGCTGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGATTTAAGATTTTTTGG‑TGGAAGatttt                                                                                                                                                              >  2:618618/1‑249 (MQ=255)
                                                                                       cgccacatgtccACCAACATTTTGCGTATA‑TCAAAGTTTTCCATACTCGCTCCCGGCCCGTTTTTTCCATTGTT‑ATGTTTCAGCTAAC‑GTGGATTGTTTACGGCCACCTGGG‑TGGATTT‑A‑GTT‑AAGTGGTAAT‑CGGGTTTTTTTTACTACTGTTGAA‑GAAAAAAAAAAAAATCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGattatt                                                                                                                                                             <  1:751176/240‑1 (MQ=255)
                                                                                         ccACAAATTCACCGACATTTTACGCATA‑TAAAATTTTTCCAACCCCCCCCCCGGCCC‑TTTTTTTTATTGTTTATCGTTCAGGTAACGGGGGATGGTTATCGGCAACGGGGG‑TG‑TTTT‑ACGTT‑A‑GGGGTGAT‑CAGGTTTTTTTTACAGTTGTTGGA‑GAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTca                                                                                                                                                           <  1:269154/251‑1 (MQ=255)
                                                                                                        cATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTCGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtt                                                                                                                                            >  1:308637/1‑251 (MQ=255)
                                                                                                         tttttACGCATA‑TAAAAGTTTCCCATCCTCGCCCCCGGCCCGTTTTTTTATTTGTT‑ATCGTTCAGTTACCCGTGGATGGTTATCGGCACCTGGGG‑TT‑TTTT‑ACGTT‑G‑GTGGTGAT‑AGGGTTTTTTTTACAACTGTTGGA‑GAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtt                                                                                                                                            <  1:646041/249‑1 (MQ=255)
                                                                                                               cGCATA‑TCAAAGTTTCCCATCCCCGCTCCCGCCCCGTTCTTTTCTTTTTT‑ATCGTTCAGTTACCCGTGTATGTTTATCGGCAACGCGGG‑TG‑TTTT‑CCGTT‑A‑GTGGTGAT‑CAGGTTTTTTTTACACCTTTTTGA‑AAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                                                                                                                     <  1:293011/251‑1 (MQ=255)
                                                                                                                         aaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACCGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTg                                                                                                                            >  1:251610/1‑251 (MQ=255)
                                                                                                                                 catccgcgcccccGCCCCTTTCTTTTCTTTGTA‑ATCGTTCAGGTGACGGTGGTTGGTTATCGACAACGCGT‑‑TG‑ATTTTATGTT‑T‑GTGGTGAT‑CAGTCTTTTTTGACAACTGTTTGA‑AAAGAAAAAGGAAAACCGCCGATCCTGTCCACCGCACTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCATTTGCCGCTGGACGTTCt                                                                                                                    <  2:188318/241‑1 (MQ=255)
                                                                                                                                   cactcccgcccGGCCTGTTTTTTTCATTTTT‑ATCTTGCGGGTAACGGTGGAGGTTTAGCGG‑AACAGGGGGTG‑TTTT‑ACGTT‑A‑GTGGT‑ATACAGGCTTTTTTTAAAACTGTTTGA‑GAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTTAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                                                                                                                  <  1:4176/243‑1 (MQ=255)
                                                                                                                                           cccggcctttttttttCATGTTT‑ATCGTTGGGGTACCGGTGGTTGGTTTTCGGCAACCGGGG‑TT‑ATTA‑CCTTT‑G‑GTGGTGAG‑CAGGTTTTTTTAACAA‑TGTTTGAAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt                                                                                                          <  2:143354/239‑1 (MQ=255)
                                                                                                                                             cGGCCCGTTCTTTTTATTTTT‑TTCTTTCAGGTAACCGTGGATGGTTATCGGAACCTGGGT‑TG‑TTTT‑ACGTT‑A‑GTGGTGAT‑CGGTTTTTTTTTACAACTGTTTGA‑GAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtc                                                                                                        <  1:529471/251‑1 (MQ=255)
                                                                                                                                                   ttcggttctaTTGTT‑ATCGTTCAGGTACCGGGGGTTGTTTATCGGCACCTGGGG‑TG‑ATTT‑CTTTT‑A‑GTGTTGAT‑CGGGTTTTTTTAACAACTGTTGGA‑AAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa                                                                                                   <  1:98970/241‑1 (MQ=255)
                                                                                                                                                            aTTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTTTCAGACGCTtctccaa                                                                                                   >  2:98970/1‑238 (MQ=255)
                                                                                                                                                                   ttcgttcaggaagccttggttttttATCGGCAACTGGGG‑TG‑ATTT‑AGGTT‑G‑GGGGTGAT‑CGTGTTTTTTTTACAACTGTTAGA‑GAAAAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGc                                                                                             <  2:102455/218‑1 (MQ=255)
                                                                                                                                                                   aTCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGc                                                                                             >  1:102455/1‑241 (MQ=255)
                                                                                                                                                                     gtttCAGGTACCGGGGGATGTTTATCGGAACCTGGGG‑TT‑ATTT‑ACTTT‑T‑GTGGTGAT‑CAGTTTTTTTTTACACCTTTTTGA‑GAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCtt                                                                                 <  1:175818/249‑1 (MQ=255)
                                                                                                                                                                     ggttcagttaccgggggATGGTTATCGACACCTCGGG‑TG‑TTTT‑ACGTA‑A‑GGGGTGAC‑CGGGCTTTTTTTAAAACTGTGGGA‑GAGAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCtt                                                                                 <  1:507844/236‑1 (MQ=255)
                                                                                                                                                                      gTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GCGGTGAT‑AAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAACGAAAACCGCCGATCCTGTCCACCGCATTACTGCCAGGTAGTGGACAAGACCGGCGGTCCTCAG‑GTTTTTGGCTGAAAGATTATTTAGCAGTTGCCAGCCTTTTACCTTTTGCCGCTGGTCGTTCTCTCAGACCCTTCTCAACATTAGCCTTTAATTGCttt                                                                                >  2:281205/1‑251 (MQ=255)
                                                                                                                                                                        tCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑GG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCCTTTTACCTTTTGCCGCTGGACGTTCTGTCACACGCTTTTCAAAATTAGCATTAAATTGCttttt                                                                              >  1:817585/1‑251 (MQ=255)
                                                                                                                                                                               aCCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGCAGTGGACAAGACCGGCGGTCTTAAG‑CTTTTTGGCTGAAAGATTATTCAGAAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCCGAAGCTTCTCAAAATTTGCATTAAATTGCTTTTTTTGTTcc                                                                       >  1:404411/1‑251 (MQ=255)
                                                                                                                                                                                  gTGGATGTTTATGGGCATCTGGGG‑T‑‑ATTT‑TCGTTTA‑GTGGTGAC‑CGGGCTTTTTTTAAAACTGTTTGA‑GAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGc                                                                    <  1:842251/251‑1 (MQ=255)
                                                                                                                                                                                  gTGGATGGTTATCGGCAGCGCGGT‑TT‑ATTT‑CCGTT‑A‑GTGGTGAT‑CAGTTTTTTTTTACACCTGTTTGA‑GAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGc                                                                    <  1:215168/251‑1 (MQ=255)
                                                                                                                                                                                   tGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTTGCTGAAAGATTATTTAGCAGTTGCAGGCCTTTTACCTTTTGTCGCTGGACGTTCTGTCAGACGCTTTTCAAAATTAGCTTTAAATTTCTTTTTCTGtttagggg                                                                   >  2:442422/1‑245 (MQ=255)
                                                                                                                                                                                     gATGTTTATCGGCACTTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑AAGGTTTTTTTTAAAACTGTTTGA‑AAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTc                                                                 <  1:320024/251‑1 (MQ=255)
                                                                                                                                                                                        ggTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAgg                                                              >  1:52986/1‑251 (MQ=255)
                                                                                                                                                                                         gTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCGTCAgg                                                              >  1:847783/1‑250 (MQ=255)
                                                                                                                                                                                               ggCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGGGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTTCCTTTTGCCGCTTGACGTTCTGTCAGACGCCTCTCAAAATTAACATTAAATTGCCTTTTCTGTTCCGGCGTCCGGGtgttgt                                                       >  2:405286/1‑251 (MQ=255)
                                                                                                                                                                                                            g‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCCTTTGCCGCTGGACGTTCTGTCAGACGCTTTTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCt                                           >  1:340743/1‑251 (MQ=255)
                                                                                                                                                                                                              aTTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑GTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTg                                          >  1:536052/1‑251 (MQ=255)
                                                                                                                                                                                                                tt‑ACGTT‑A‑GAGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAGGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCGGAAAGATTTTTCGGCAGTTACAGGCATTTTTCCTTTTGCCGCGGGACGTTTTGTCAGACGCTTCTCAAAATTAGCTTTAAATTGTTTTTTTTGTTCCGGCGTCAGGGTGTTGTAAATTTTGTTCTggg                                        >  1:798942/1‑251 (MQ=255)
                                                                                                                                                                                                                     gTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCGGGTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTc                                    >  1:740803/1‑251 (MQ=255)
                                                                                                                                                                                                                     gTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGACTTAAA‑TTTTTTGGATGAAAAAATATTTAGCAGTTGCAGGCATGTTTCCTTTTGCCGGTGGGAGGTCTAGCAGACGGTTCTCAAAATTAGCATTACATTGCTTTTTCTGTTCCGGCGTTCTGATGTTTTTAATTTTTTTCTGGGtttt                                    >  2:488026/1‑250 (MQ=255)
                                                                                                                                                                                                                          gagtgttgacaagttttttttaACAACCTTTGGA‑GAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTcc                                   <  2:251610/235‑1 (MQ=255)
                                                                                                                                                                                                                              ggtgattggcttttttttACACTTGTTGGA‑AAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCAtgtg                              <  1:775729/240‑1 (MQ=255)
                                                                                                                                                                                                                                    cAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTTAATTGCTTTTTTTGTTCCGGCGGCAGGATGTTGTAAATTTTGTTCTGGGTTTTCATGTG‑CGcccg                       >  2:570584/1‑249 (MQ=255)
                                                                                                                                                                                                                                    cAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTG‑CGcc                         >  1:511992/1‑249 (MQ=255)
                                                                                                                                                                                                                                       gCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTTGCTGAAAGATTATTTAGCCATTGCAGGCATTTTAACTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAAACTTAAATTGCTTTTTCTGTTCCGGCGTCAGGGTGTTGTAAATTTTGTTCTGGGTTTCCATGTG‑CGCCAGCAt                    >  2:645886/1‑251 (MQ=255)
                                                                                                                                                                                                                                           tttttACAACTTTTGGA‑AAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTG‑CGCCAGCATGtt                 <  1:884190/250‑1 (MQ=255)
                                                                                                                                                                                                                                           ttttaACAACTGTTTGA‑GAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTG‑CGCCAGCATGtt                 <  1:818512/250‑1 (MQ=255)
                                                                                                                                                                                                                                              ttACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTTGCCT‑AAAGATTATTCAGCAGTTTCAGGCCTTTTAACTTTTGCCGCTGGACGTTTTTTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTTAATTTTGTTCTGGGTTTCCCT‑TGTCGCCAGCATGTTCGCt             >  2:450941/1‑251 (MQ=255)
                                                                                                                                                                                                                                               tACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTTGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTTAATTGCTTTTTCTGTTCCGGCGTTAGGATGTTTTAAATTTTGTTCTGGGTTTCCATGTT‑CGCCAGCATGTTTGCtt            >  2:771478/1‑251 (MQ=255)
                                                                                                                                                                                                                                                          ggaaaagagaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTG‑CGCCAGCATGTTAGCTTTGCGCTGttc  <  1:65938/247‑1 (MQ=255)
                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    
CGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTC‑GCCACAGATTCACCGACATTTTACGCATA‑TCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTT‑ATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGG‑TG‑ATTT‑ACGTT‑A‑GTGGTGAT‑CAGGCTTTTTTTACAACTGTTTGA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTG‑CGCCAGCATGTTAGCTTTGCGCTGTTC  >  NC_000913/1824808‑1825298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑