Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,7020CA30.4% 75.5 / 11.7 48A37S (GCG→TCG) gadXDNA‑binding transcriptional dual regulator GadX
Reads supporting (aligned to +/- strand):  ref base C (25/7);  new base A (1/13);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 8.56e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCT  >  NC_000913/3665453‑3665936
                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              
cAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑Gc                                                                                                                                                                                                                                                >  1:69990/1‑250 (MQ=255)
        aaTGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAc‑c                                                                                                                                                                                                                                        >  1:300982/1‑249 (MQ=255)
             tctTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCAc                                                                                                                                                                                                                               >  2:883627/1‑251 (MQ=255)
                tttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCg                                                                                                                                                                                                                             <  1:445566/250‑1 (MQ=255)
                                        aaGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCAt                                                                                                                                                                                                   >  1:217196/1‑251 (MQ=255)
                                                tATTCTGCGATAGTTGCGCACCTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCTGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACAACCTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAACAC‑C‑A‑GGTCACCGCCATTAAAATATCG‑ATCTTCCCCATTAACCATg                                                                                                                                                                                           >  2:535776/1‑251 (MQ=255)
                                                 aTTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATgg                                                                                                                                                                                          >  2:343464/1‑251 (MQ=255)
                                                 aTTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTCGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATgg                                                                                                                                                                                          >  1:802842/1‑251 (MQ=255)
                                                    cTGCGAGAGTTGCGCAACTTCGTGGAAAACAGGAATATCTCTACTACAGTTGTGAAAGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCAACGGGAGAAATAA‑TAAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCGTTAACCATGGTg                                                                                                                                                                                        >  2:875548/1‑250 (MQ=255)
                                                        gATATTTGCGCAACTTCCTGAAAAACAGGAATATCTTTACTACAGGGATGAACGAGTAATAAAAAATCTTTTTTATGTG‑CATGAAGATTTAATGCCCCCCCCTTGAGCATCGGAAAAAATAA‑TAAAAGCGTGCCCCTTGAC‑ACAAATACAAATTTT‑T‑A‑AACACCCTTATC‑TCCTCAAA‑TTGGG‑CTTGCACCC‑GAAAAAAC‑C‑A‑GGTCACCGCCATTa                                                                                                                                                                                                                       <  2:803137/216‑1 (MQ=255)
                                                        gATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTa                                                                                                                                                                                                                       >  1:803137/1‑216 (MQ=255)
                                                         aTAGTTGCGCAACTTCCTGAAAAACAGAAATATCTCTACAACAGTGATAAAGGAGTAATAAAAAAACTTTTTTATGTG‑CATGAAGATTTAATCCCTCCTCCTGGAGTATCGGAAAAAAAAA‑AGAAAGCGTCTCCCTTGAC‑ACAAATACAAAATTT‑C‑C‑AACCCACTTATC‑TACTCAAA‑TTTGG‑CTTGCACCC‑ACAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAata                                                                                                                                                                                  <  2:69990/251‑1 (MQ=255)
                                                                   aaCTTCCTGAAAAACAGGAATATCTCTACTACAGAGATGAACGCGTAATGAAGAAAATTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTc                                                                                                                                                                        >  2:187527/1‑251 (MQ=255)
                                                                    aCTTCATGAAAAACAGGAATATCTCTACTACCGCGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCATTGAGAATCGGGAGAAATAA‑TGAAAGCGTGACCCTTGAC‑ACGAAGACAAAATTT‑T‑C‑AAAACACTTAAC‑TCCTCGAA‑TTTGG‑CTT‑CAACCAGAAACAAC‑A‑A‑GTCCACCGCCATTAAAATAGCGCAT‑TTCACCATTAAAAATGGAGCGAAGATATTTATGTCt                                                                                                                                                                       >  2:844632/1‑251 (MQ=255)
                                                                       tCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGc                                                                                                                                                                    >  1:866004/1‑251 (MQ=255)
                                                                              aaaCATGAATCTCTCTACTACAGTGATAAACGAGTAATAAAAAATCTTTTTTA‑GTGGAAAAAAGTTTTAATCCCCCCCCCTGGAACCTGGGGAAAAATAA‑TAAACGCTTGCCCTTTGAC‑ACAAATACAAAATTT‑C‑A‑AACCCACTAATC‑ACCACGAT‑TTTGT‑TTTGCCCCC‑GAAAAACC‑C‑G‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCa                                                                                                                                                             <  2:193261/251‑1 (MQ=255)
                                                                                        atCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAGC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                                                                                                                   >  1:581714/1‑251 (MQ=255)
                                                                                              actacAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACGCACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGAATACGCAATTAGACAATTCCCAt                                                                                                                                             >  1:665991/1‑251 (MQ=255)
                                                                                                tacAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                                                                                                                           >  1:423351/1‑251 (MQ=255)
                                                                                                         gaaggAGTAATGAAGAAGTTTTTTTATGAG‑CGTGAATATGTAATGCCTCCTCGTGGAGCATCGGGAAAAAAAA‑TGATATCTCTTCCCTTGAA‑ACGAATAAAAATTTT‑T‑A‑AACAAATTTTTC‑CAGACAAT‑TTTGG‑GGTGCCTCC‑GCAAAAAC‑C‑G‑GGAACCCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCGTGCATGCGCAATTAGACAATTCCCATGTAGGGATTg                                                                                                                                   <  2:704165/246‑1 (MQ=255)
                                                                                                             gAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                                                                                                                >  1:46178/1‑249 (MQ=255)
                                                                                                                       aaattttttttATTTG‑CATGAAGATTTATTGCCTCCTCCTTGGGCACCGGAAAAAATAA‑TGAAAGCTTGTCCTTGGAC‑ACAAAAACAAAATTT‑C‑C‑ACCCCATTTATC‑TACTCGAT‑TTGGG‑CTTGCACCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAat                                                                                                                    <  1:76385/246‑1 (MQ=255)
                                                                                                                                    tgtacATGAAGTTTTAACGCCTCCTCCTGGGGCATGGGAAAAAAAAA‑TAAAGGCGTGCCCCTGTAC‑AAAAAAACAAATTTT‑A‑C‑AACCCCCTTCTC‑TACTAAAA‑TTTGC‑CTTGAACCC‑G‑AAAAAC‑C‑AAGTTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAAc                                                                                                         <  2:300982/246‑1 (MQ=255)
                                                                                                                                     gtacgtGAAAATTAAATGCCCCCTCCTTGAACATCGGGA‑AAATAAATAAAAGCTTCTCCTTTGAC‑A‑GAAAAAAAAATTT‑TTA‑AAAACCCTTATC‑TCCTCAAT‑TTTGG‑CTTGCTCCC‑GAAAAACC‑C‑G‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAATCat                                                                                                       <  2:278785/246‑1 (MQ=255)
                                                                                                                                           aaagttttAATTCCTCCTCCTTGATCACAGGAAAAAAAAA‑TAAAAGCGTGTCCCTTAAA‑ACAAAAAAAAAATTT‑A‑C‑AACACA‑TTATCCTA‑TCGAAATTTGG‑CTTTCA‑CC‑ACAAAAAC‑CCA‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACt                                                                                                 <  2:267637/246‑1 (MQ=255)
                                                                                                                                           aaaaaTTTAATCCCCCCTCCTTGAACATGGGGAAAAAAAA‑TGAAAGCGTCTCCTTTGAC‑CCGAAGA‑AAAATTT‑TAC‑AACACATTTATC‑TCCCCGAT‑TTTGG‑TTTGCACCC‑GAAAAACC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc                                                                                                  <  1:544396/246‑1 (MQ=255)
                                                                                                                                           aaaGATTTATCGCCCCCTTCTTGAGCAGGGGGAGAAATAA‑GGAACGCGTGTCCTTGGAC‑CCAAATA‑AAAATTTCT‑A‑AACCCTCTTATC‑TCCCAGAA‑TTGGG‑CTGGATTCC‑GAAAAAAC‑A‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACt                                                                                                 <  1:535776/250‑1 (MQ=255)
                                                                                                                                               aTTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGttt                                                                                             >  1:554937/1‑251 (MQ=255)
                                                                                                                                               aTTTAATGCCTCCTCATTGGACAGGGGGAAAAAAAA‑TGAAAGCGTGCCCCTTGAC‑ACAAATAAAAAATTT‑T‑A‑AACACACTTTTC‑TACTAGAA‑TTTGG‑TTTGAATCC‑GAAAAAAC‑C‑G‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGttt                                                                                             <  1:343464/251‑1 (MQ=255)
                                                                                                                                                 ttAATCCCTCCGCCTTGAGAATCGGGAAAAATAA‑AGAAAGTGTG‑CCCTTTACCAC‑AAAAAAAAATTT‑TAC‑AACACCTTTATC‑TA‑TCGAAATTTGC‑TTTGCCTCC‑GCAAAAAC‑C‑A‑GTTAACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTtta                                                                                            <  2:605749/250‑1 (MQ=255)
                                                                                                                                                   aaTGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTttatt                                                                                          >  2:781773/1‑250 (MQ=255)
                                                                                                                                                                tGAGCATGGGAAGAAATAA‑TGAAAGCGTGCCCCTTGAC‑CCAAATAAAAAATTT‑T‑C‑AACACATTTATC‑TACTCGAA‑TTTGG‑TTTGCACCC‑GAAAAACC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCAcgcc                                                                             <  1:14318/250‑1 (MQ=255)
                                                                                                                                                                    aaTCGGGAAAAAAAA‑GGAAGGCGTTCCCCTTAAC‑ACAAATAAAAAATTT‑C‑A‑AACACTCTTTTC‑TACTCAAA‑TTTGG‑CTGCCACCA‑GAAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCct                                                                        <  2:46178/250‑1 (MQ=255)
                                                                                                                                                                       cGGGAGAGATAA‑TGACAGCGTGTCCCTTGAC‑ACGAATACACAATTT‑T‑C‑AACAAACTTATA‑TACTCGAA‑TTTGG‑CATGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCtt                                                                     >  2:199986/1‑251 (MQ=255)
                                                                                                                                                                                           gtgtCCCTTGAC‑ACGAATACAAACTTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATg                                                                                      >  2:662552/1‑215 (MQ=255)
                                                                                                                                                                                           gtgccccTTGAC‑ACAAATA‑AAAATTT‑T‑CCAACACACTTATC‑TCCTCGAA‑TTTGG‑CTTGCACCC‑GAAAAACC‑C‑G‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATg                                                                                      <  1:662552/211‑1 (MQ=255)
                                                                                                                                                                                           ggtgccccttaacccaaaTAAAAAATTT‑C‑A‑AACCCACTTATC‑TCCCCGAA‑TTTGG‑CTTGCATCC‑GAAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGata                                                   <  1:285945/235‑1 (MQ=255)
                                                                                                                                                                                             tccctttGAC‑ACAAATACAAATTTT‑T‑C‑AACACATTTATC‑TCCTCGAT‑TTTGG‑CTTGCACCC‑GAAAAACC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCaa                                                              <  1:32305/232‑1 (MQ=255)
                                                                                                                                                                                             gtCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCaa                                                              >  2:32305/1‑237 (MQ=255)
                                                                                                                                                                                               cccTTGAC‑ACAAATAAAAAATTT‑C‑C‑AACACACTTATC‑TCCTCTAA‑TTTGG‑CTTCCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGataaataa                                              <  1:298054/251‑1 (MQ=255)
                                                                                                                                                                                                     ac‑acGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTa                                         >  1:106332/1‑250 (MQ=255)
                                                                                                                                                                                                                              cacacTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGgatg                   >  2:859017/1‑251 (MQ=255)
                                                                                                                                                                                                                                 atttATC‑TACACGAA‑TTTGG‑CTTGCACCC‑GAAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAAAGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATa                <  1:781773/249‑1 (MQ=255)
                                                                                                                                                                                                                                   ttATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAAt              >  2:396349/1‑251 (MQ=255)
                                                                                                                                                                                                                                           cTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGAt       >  1:115580/1‑251 (MQ=255)
                                                                                                                                                                                                                                            ccaga‑TTTGGTCTTGCACCC‑G‑AAAAACAC‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGTGAGAACAGGATGATAATGTGTGAt       <  2:74092/247‑1 (MQ=255)
                                                                                                                                                                                                                                               aa‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGAAGTTc   >  2:184410/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 aattggggttGCACCC‑GAAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTc   <  1:396349/243‑1 (MQ=255)
                                                                                                                                                                                                                                                  tttGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCt  >  1:652525/1‑250 (MQ=255)
                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              
CAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTG‑CATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAA‑TGAAAGCGTGTCCCTTGAC‑ACGAATACAAAATTT‑T‑C‑AACACACTTATC‑TACTCGAA‑TTTGG‑CTTGCATCC‑GCAAAAAC‑C‑A‑GGTCACCGCCATTAAAATAGCG‑ATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCT  >  NC_000913/3665453‑3665936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑