BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,622,250	0	A	G	28.6%	213.1 / 18.4	98	E161G (GAG→GGG)	yjjJ	toxin YjjJ
Reads supporting (aligned to +/- strand): ref base A (11/59); new base G (28/0); total (39/59)
Fisher's exact test for biased strand distribution p-value = 6.49e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 107 total aligned reads displayed.

ACATCCGGTTGTGCTGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTGACCTGCTAATTGCT  >  NC_000913/4622001‑4622500

aCATCCGGTTGTGCTGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCggggag                                                                                                                                                                                                                                                                 <  2:135837/251‑1 (MQ=255)

 cATCCGGTTGTGCTGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑GTGGTCggggagg                                                                                                                                                                                                                                                                >  1:844223/1‑251 (MQ=255)

              tGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑GTGGGCGGGGGGGGGAATTATCAGCg                                                                                                                                                                                                                                                   >  1:53561/1‑251 (MQ=255)

               ggCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCg                                                                                                                                                                                                                                                   <  1:388485/250‑1 (MQ=255)

                   gCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGa                                                                                                                                                                                                                                              <  1:490716/251‑1 (MQ=255)

                     aGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTTGGGGGGGGGGATTATCAGCGATgggtt                                                                                                                                                                                                                                            >  2:344150/1‑248 (MQ=255)

                     aGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGc                                                                                                                                                                                                                                                    <  1:59360/243‑1 (MQ=255)

                     aGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGAtt                                                                                                                                                                                                                                            <  2:25976/251‑1 (MQ=255)

                     aGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑GTGGTGGGGGGGGGGGATTATCAGCGATgggtt                                                                                                                                                                                                                                            >  1:519115/1‑248 (MQ=255)

                     aGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑GTGGGCGGGGGGGGGGATTAtacacagcttggg                                                                                                                                                                                                                                            >  2:59360/1‑239 (MQ=255)

                              gTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCacaa                                                                                                                                                                                                                                   <  1:820835/251‑1 (MQ=255)

                                  ggTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAacac                                                                                                                                                                                                                                <  1:159362/250‑1 (MQ=255)

                                  ggTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACATCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAacac                                                                                                                                                                                                                                <  2:844223/250‑1 (MQ=255)

                                   gTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACAccc                                                                                                                                                                                                                              <  1:208006/251‑1 (MQ=255)

                                        aGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGGGGGGGATTTTTAGCGGTGGATTACTGCACAACACCCCGCgg                                                                                                                                                                                                                         >  1:694324/1‑251 (MQ=255)

                                          gcgcAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑aa                                                                                                                                                                                                                      <  1:341217/251‑1 (MQ=255)

                                          gcgcAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑aa                                                                                                                                                                                                                      <  1:59879/251‑1 (MQ=255)

                                             cAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAAtt                                                                                                                                                                                                                   <  2:205044/251‑1 (MQ=255)

                                                aTGGCGGCGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGGCCGATCTCCGACCGCAGGGCTTAGGAGGGCGCGCGTGGGGCAGTAAGTTAGCCGCGCAACTGAATCTGGCTGATGATAGCCGTCTCTGGGAGGAAGAAGGTGTGCGCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCATCGATTGATTACTGCACAACACCCTGCTG‑AAATTCct                                                                                                                                                                                                                <  1:177991/251‑1 (MQ=255)

                                                  ggCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCctc                                                                                                                                                                                                               <  1:631236/250‑1 (MQ=255)

                                                    ggacgaACAGTGGTTTGAGGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGGGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGT‑TTGG‑TTGGTCGGGGAGGGTAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTgg                                                                                                                                                                                                            <  2:718634/250‑1 (MQ=255)

                                                    cgacgaACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGTGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑GTGGGCGGGGGGGGGGATTTTTAGCGATGGATTACTGCACAACAACCCGCGG‑AAATTCCTCTgg                                                                                                                                                                                                            >  1:56106/1‑251 (MQ=255)

                                                      acgaACAGTGGTTAGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCACCTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAAGTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGAt                                                                                                                                                                                                          <  2:110597/251‑1 (MQ=255)

                                                        gaACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCa                                                                                                                                                                                                        <  1:781629/251‑1 (MQ=255)

                                                        gaACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCa                                                                                                                                                                                                        <  2:7022/251‑1 (MQ=255)

                                                        gaACAGTGGTTTGATGGTTGGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCa                                                                                                                                                                                                        <  2:619297/251‑1 (MQ=255)

                                                          aCAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCTGGGGGGGGAATTATCCGCGAATGATTACTGCACCACACCCCCCGG‑AAATTCctctcgcgcaa                                                                                                                                                                                                       >  1:857262/1‑243 (MQ=255)

                                                            aGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCaaaaa                                                                                                                                                                                                    <  1:668816/251‑1 (MQ=255)

                                                             gTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGGCCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAAc                                                                                                                                                                                                   <  2:167292/251‑1 (MQ=255)

                                                               ggTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTc                                                                                                                                                                                                 <  1:458693/251‑1 (MQ=255)

                                                                    gagggTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTGTTAGTGCGCGCGTGGGGCAGGGAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTGAACGGTGAATATACTTGCGG‑TTGG‑GTGGTCGGGGATGGGAATTATCAGCGATGGATTACGGCACAACGCCCTGCGG‑AAATTCCTCTGGATCAAAAACTCG‑ccc                                                                                                                                                                                            <  2:756648/247‑1 (MQ=255)

                                                                     aTGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTTGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCAt                                                                                                                                                                                          <  2:658576/251‑1 (MQ=255)

                                                                        gTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTAc                                                                                                                                                                                       <  1:847353/251‑1 (MQ=255)

                                                                                    aTTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGGGGGGAATTATTAGCGAGGGAATATAGTACAACACCCTGCGG‑AAATTCCCCTGGAACAAAAAATCA‑CCCCCTACGAAAA‑GCTGGc                                                                                                                                                                           >  1:877744/1‑250 (MQ=255)

                                                                                       tGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑GTGGTCGGGGGGGGGGATTATTAGCGATGGGTTTCTGCACAACAACCTGCGG‑GAATTCCTCTGGATAAAAAACACA‑CCCCTTTCGaaaaactgggaagg                                                                                                                                                                        >  1:355094/1‑239 (MQ=255)

                                                                                        gACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTTG‑TTGGTCGGGGGGGGGAATTATTAGCGGTGGATTACTGCACAACAACCTGCGG‑AAAATCCCCTGGATCAAAAAATCAACCCAT‑ACGAACA‑GCTGGCAAggg                                                                                                                                                                      >  1:79461/1‑249 (MQ=255)

                                                                                        gACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTTG‑GTGGGCGGGGAGGGGGATTATTAGCGGTGGATTTCTGCACAACACCCCGCGGGAAATTCC‑CTTGATCAAAAAATCA‑CCCATTACCAACaacctgcaaggg                                                                                                                                                                       >  1:233566/1‑240 (MQ=255)

                                                                                        gACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGt                                                                                                                                                                       <  2:880159/250‑1 (MQ=255)

                                                                                         aCCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGAAGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGa                                                                                                                                                                     <  2:825582/251‑1 (MQ=255)

                                                                                           cGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑GTGGTCGGGGGGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACACA‑CCCATTACGAACA‑GACGGCAAGGGATg                                                                                                                                                                   >  1:63982/1‑251 (MQ=255)

                                                                                                 cctagcgcttggCTTATTAGTGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTAACAGATAGTATGCGTCTCTGGCAGGAATGAGATGTTCACTACGTCCAGACCGTATTTAACGGCGAATGCGCTGGCGG‑TTAG‑TAGGCCGGGGAGGGTATTTAGCAGCGATGAATTACGACACATCACCCTGAGG‑AAATACCGCTGGAACAGAAACTCA‑CCCATGTCGAAGC‑GCTGGCAATTGACGCAcg                                                                                                                                                               <  2:462546/239‑2 (MQ=255)

                                                                                                 ccGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACt                                                                                                                                                               <  1:630159/249‑1 (MQ=255)

                                                                                                  cGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATGACGAACA‑GCTGGCAAGTGATGCACTGGc                                                                                                                                                            <  2:276900/251‑1 (MQ=255)

                                                                                                  cGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGGCGGGGAGGGGAATTATCAGCGATGGGTTAATGCACAAAACCCCTCGG‑AAATTCCTCTGGATCAAAAAACCA‑CCCATTACGAACA‑GCCGGCCAGGGGTGCACTGGc                                                                                                                                                            >  1:227585/1‑251 (MQ=255)

                                                                                                    aCCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAg                                                                                                                                                          <  2:355094/251‑1 (MQ=255)

                                                                                                            gCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAAtt                                                                                                                                                 <  1:426752/251‑1 (MQ=255)

                                                                                                                  tAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGGGGGGGATTTTCAGGGATGGATTACTGCCAAACAACCCGCGG‑GAATTTCTCTGGATCAAAAACTCA‑ACCATTACGAACA‑GCTGGCAAGTGATGCACTGGCCGGGG‑AAAATGTgggg                                                                                                                                           >  1:59631/1‑250 (MQ=255)

                                                                                                                     ggCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑GTGGTCGGGGAGGGGAAATATCAGCGATGGGTTACTGCACAAAACCCCGCGG‑AAAATTCTCTTGATCAAAAAATCA‑CCCCTTAAGAACA‑GCTGGCAAGTGATGCACTGGGAGGGG‑aaaatttggggtct                                                                                                                                        >  1:214271/1‑240 (MQ=255)

                                                                                                                            gTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑GTGGTCGGGGGGGGGAATTATCAGCGGCGGATTACTGCACAAAACCCCGCGG‑GAATTCCTCTGGGTCAAAAACTCAACCCAT‑ACGCACA‑ACCGGCAAGAGAAGCACTGGCAGGGG‑GAATTGTGGGGTCTTCTgcgg                                                                                                                                 >  2:269526/1‑251 (MQ=255)

                                                                                                                            gTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑GTGGTCGGGGGGGGGAATTATCAGCGATGGATTTATGCACAACACCCTGCGG‑AAATTCCTCTGGGTCAAAAACTCC‑ACCATTACGAACA‑GCTGGCAAGGGATGCAATGGCAGGAG‑AAATTGTGGGTTCTTCTgcgg                                                                                                                                 >  1:642917/1‑251 (MQ=255)

                                                                                                                             tGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGGATTATCAGCGCTGGGTTACTGCACAACAACCTGCGG‑AAAATCCTCTGGATCAAAAAATCA‑CCCATTTAGAACA‑GCTGGCAAGTGATGCACAGGCAGGAG‑AAATTGTGGGTTCTTCTtcggg                                                                                                                                >  1:541525/1‑251 (MQ=255)

                                                                                                                              ggtggaGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCGGAGCGTATTTAACGGGTAAGATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACCCCCGGCGG‑AAATTCCTCTGGATCAAAAACGCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGGTCTGCTgcggg                                                                                                                                <  2:469330/245‑1 (MQ=255)

                                                                                                                              ggggCAGGAAGTGAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGTCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACTCCCTGCGG‑TAATTCCTCTGGATCAAAAACTCA‑CCCAGTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTgcgggc                                                                                                                               <  2:216947/251‑1 (MQ=255)

                                                                                                                               gggCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGGGTTGG‑CGGGGGGGGGGGTTATTAGCGATTGGTTTCTGCCCAACACCCCGCGG‑GAATTCCTCTGGGTCAAAAAATCA‑CCCATTCCGAACC‑ACCGGCAAGTGATGAACTGGCCGGAG‑CAATTGTGGGTTTTTTTtcgggcg                                                                                                                              >  2:194425/1‑251 (MQ=255)

                                                                                                                                 gCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGATCGG‑TTG‑TCCGGGGGGGGAGTTATATGAGGTGGAGAACTGCACAACAACCTTCGG‑CAAATCCCCTGGATCAAAAACTCG‑CCCATTACGAACA‑GCCGGCAAGTGATACACAGGAAGGAGGACATT‑TGGGTTCTTATccgggcggg                                                                                                                            >  1:455558/1‑250 (MQ=255)

                                                                                                                                  cAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGgcggcg                                                                                                                           <  1:20092/251‑1 (MQ=255)

                                                                                                                                   gggAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGa                                                                                                                          <  2:151414/250‑1 (MQ=255)

                                                                                                                                   aGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGGGGGGGATTATCAGCGGTGGATTACTGCACAACAACCTGCGG‑AAAATTCTCTGTGGCAAAAAATCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑GAATATTGGGGTTTTCTGCGGggggcgg                                                                                                                          >  1:73348/1‑250 (MQ=255)

                                                                                                                                    ggAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGag                                                                                                                         <  1:424929/251‑1 (MQ=255)

                                                                                                                                                 gcAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTAATGCACAACACCCTGCGG‑GAATTCCTCTGGGTCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAGGAAATGGTGGGT‑CTTCTGCGGGCGGCGAGCAGCCACAAA‑ttt                                                                                                           >  1:837715/1‑251 (MQ=255)

                                                                                                                                                       gAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTTG‑TTGGTCGGGGAGGGGGATTATTAGCGGTGGATTAATGCACAACACCCTGCGG‑AAATTCCCCTGGATCAAAAACTAA‑CCCCTTACGAAAA‑GCTGGCAAGTGACGTCCTGGCAGGAG‑AAAATGGGGGGTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑ATTACctact                                                                                                    >  1:723213/1‑251 (MQ=255)

                                                                                                                                                             gactgaTGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATg                                                                                                 <  2:214271/248‑1 (MQ=255)

                                                                                                                                                             gactgaTGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGc                                                                                                <  1:73200/249‑1 (MQ=255)

                                                                                                                                                              actgaTGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGGGGGGGATTATCAGCGATGGGTTACTGCACAACACCCCGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGGGATGCACCGGCCGGGG‑AAATTGTGGGGTCTTCTGCGGGGGGGGCGCAGCCA‑AAA‑TTTACCTACTATGcacc                                                                                             >  1:140040/1‑250 (MQ=255)

                                                                                                                                                              actgaTGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGGCGGGGAGGGGAATTAACAGGGATGGATTACAGCACAACACCCCGCGG‑AAATTCCTCTGGGTCAAAAAATCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGGG‑AAAATGTGGGGTCTTCTGCGGGGGGCGAGCAGACA‑AAA‑ATTTCCTACTATGcac                                                                                              >  1:760489/1‑250 (MQ=255)

                                                                                                                                                                       atgcgtgtcgggaaGGAAGACGATGTGCGAAACGCCCTGTGCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAGTTAGCGGCGATGGTTTACTGCGCAACTCCCTGCGG‑AAATTCCTCTGGATCAAAAACGCA‑CCCATTACGAACA‑GCTGGCAAGTGATTCTCTGGCAGGAG‑AAATTGAGGGTTCTTCTGCGGGCGGTGAGCTGGCA‑GAA‑TTTGCCTACTATGCACAGACGCCgg                                                                                     <  2:809182/237‑2 (MQ=255)

                                                                                                                                                                       ataCGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTTACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTAGGCACAAACGCCGTc                                                                                    <  1:542075/251‑1 (MQ=255)

                                                                                                                                                                       ataCGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCTCAAACGCCGt                                                                                     <  2:690493/250‑1 (MQ=255)

                                                                                                                                                                       ataCGGCTCTGGCAGGAAGAAGATGGGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTc                                                                                    <  2:277445/251‑1 (MQ=255)

                                                                                                                                                                           gTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑GTGGTCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAACACCCTGCGGGAAATTC‑TCTGGATCAAAAACTCA‑CCCATTAAGAAAA‑GCTGGCAAGGGATGCACTGGCAGGAG‑AAATTGTGGGGTCTTCTTCGGGGGGCGAGCAGCCA‑AAA‑TTTACCTACTCTTCACAAACCCCGTCAGGc                                                                                >  1:336404/1‑251 (MQ=255)

                                                                                                                                                                              tctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGGGGGGGATTATCAGCGATGGGTTACTGCACAACACCCTGCGG‑AAATTTCTCTGGATCAAAAACTCA‑CCCATTACGCACA‑GCTGGCAAGTGATGCACTGGCAGGGG‑AAATTGTGGGGTCTTCTGCGGGCGGCGGGCAGCCA‑AAA‑TTTTCCTACTATGCACAAACGCCGGCAGGGAAt                                                                             >  1:845866/1‑251 (MQ=255)

                                                                                                                                                                               ctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCa                                                                               <  2:79461/248‑1 (MQ=255)

                                                                                                                                                                                 tgcatgaagaatcggtgCTTTACGCCCTGACCGTATTTAACGGGGAATATACTGGCGG‑GTGG‑TTGGTCGGGGAGGGGAATTATCCGCTATGGATTACTGCTCAACTCCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACG‑GCTGGCAAGTGATGCACTGGCAGTAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGATCAGCCA‑AAA‑TGTACCTACTATGCTCAAACGCCGTCAGGCAATa                                                                            <  2:179201/235‑1 (MQ=255)

                                                                                                                                                                                 ggCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑GTGGTCGGGGGGGGGGATTATACGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCCTTACGAACA‑GCTGGCAAGTGAAGCACTGGCAGGAG‑AAATTGTGGGGTCTTCTGCGGGCGGGGGGCAGCTA‑AAA‑ATTACCTACTATTCACAAACGCCGTCAGGCAATaa                                                                           >  1:321241/1‑250 (MQ=255)

                                                                                                                                                                                      gaagaaGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑GTGG‑GTGGTCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑GAATTTCTCTGGGGCAAAAAAACA‑CCCATTACGAACA‑GCTGGGAAGTGATGCAATGGCAGGAG‑AAATTGTGGGGTCTTCTGCGGGGGGGGGGCAGCCA‑AAA‑TTTTACTACTATGCACAAACGCCGTCAAGCAATaaaaaagt                                                                     >  1:567212/1‑246 (MQ=255)

                                                                                                                                                                                        agaagaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACAt                                                                       <  1:129129/247‑1 (MQ=255)

                                                                                                                                                                                            gaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGt                                                               <  2:887874/251‑1 (MQ=255)

                                                                                                                                                                                             aTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATTAGCGCTGGATTATTGCACAACACCCTGCGG‑AAATTCCTCTGGGTCAAAAAATCA‑CCCATTACGAACA‑ACTGGCAAGTGATGCACCTGCAGGGG‑AAAATGTTGGGTCTTCTTCGGGGGGCGGGCAGCCA‑AAA‑TTTTCCTACTATTCCCAAACGCCGGCCGGCAATAAACAATTGTTgggg                                                              >  1:665274/1‑249 (MQ=255)

                                                                                                                                                                                             aTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑GTGGTCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCCATACGAACA‑GCTGGCAAGGGGTGCACTGGCAGGAG‑AAATTGTGGGGTCTTCTGCGGGCGGCGGGCAGCCC‑AAA‑TTTAACCACTATTCACAAACGCCGTCAGGCAATAAACATGTGTTgggg                                                              >  1:524176/1‑249 (MQ=255)

                                                                                                                                                                                              ggtgCACTACGCCCTGAACGTATTTAACGGTGAATATACTGGAGG‑TTGG‑TTGGTCGGGGAGGGGAATGATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGa                                                             <  2:857262/250‑1 (MQ=255)

                                                                                                                                                                                               gtgCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTTG‑TTGGGCGGGGGGGGGAATTATCAGCGATGGATTACTCCACAACACCCCGCGG‑GAATTCCTCTGGGTCAAAAACACA‑CCCCTTACGAAAA‑GCTGGCAAGTGATTCACTTGCCGGAG‑AAATTGTGGGGTTTTCTTCGGGGGGCGGGCAGCCA‑AAAATTTAC‑TAATATGCACAAACGCCCGCAGGCCATAAACATGTGTTGGTgg                                                             >  1:607753/1‑249 (MQ=255)

                                                                                                                                                                                               gtgCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGGCGGGGGGGGGAATTATCAGCGGTGGATTAATGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACCAACA‑GCTGGGAAGTGATGCACCGGCAGGAG‑AAATTGTGGGTTCTTCTTCGGGGGGGGGGCAGCCA‑AAA‑ATTTCCTTCTATGCACAAAAGCCGCCCGGCAAAAAAAATGTGTTGGTGa                                                             >  1:610223/1‑250 (MQ=255)

                                                                                                                                                                                                tgCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTTG‑GTGGGCTGGGGGGGGGATTATCAGCGGTGGATTAATGCACAACACCCCGCGG‑AAAATCCTCTGGATCAAAAAATCA‑CCCATTACGAACACGCGGGAAAG‑GGTGCACAGGCAGGAG‑AAAATGTGGGTTCTTCTGCGGGGGGCGGGCTGCCA‑AAA‑TTTACCTACTCTGTACAAACGCCGTCACGGAAAAAAAATGTGtttgggaa                                                            >  2:21034/1‑244 (MQ=255)

                                                                                                                                                                                                    ctACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAAtt                                                         <  1:524446/249‑1 (MQ=255)

                                                                                                                                                                                                      aCGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTc                                                        <  2:74593/248‑1 (MQ=255)

                                                                                                                                                                                                      aCGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTc                                                        <  1:269526/248‑1 (MQ=255)

                                                                                                                                                                                                      aCGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCAcc                                                     <  1:757774/251‑1 (MQ=255)

                                                                                                                                                                                                         cccTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTa                                                  <  2:233566/251‑1 (MQ=255)

                                                                                                                                                                                                                               atatACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGcc                            <  1:737362/251‑1 (MQ=255)

                                                                                                                                                                                                                                   aCTGGCGG‑TTGG‑TTGGGCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCGTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAAc                         <  2:388468/250‑1 (MQ=255)

                                                                                                                                                                                                                                         gg‑ttgg‑ttGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTgggg                  >  1:85266/1‑251 (MQ=255)

                                                                                                                                                                                                                                          g‑ttgg‑ttgGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCATCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGt                 <  2:607753/251‑1 (MQ=255)

                                                                                                                                                                                                                                            ttgg‑ttggACGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGCTCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTg                >  2:147401/1‑251 (MQ=255)

                                                                                                                                                                                                                                               g‑ttggtCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGAGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTgccc             >  2:121939/1‑248 (MQ=255)

                                                                                                                                                                                                                                                       ggggaggggaATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTGACCTGCTAAt      >  1:516932/1‑251 (MQ=255)

                                                                                                                                                                                                                                                       ggggaggggaATTATCAGCATTGGATTACTGCACAACTCCCTGCGG‑AGATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGATATGCACTGGCAGGCG‑AAATTGTGGGTTCTTCAGAGGACGGCGAGCAGCCA‑AAC‑TTTACCTACTATGCACAACAGCCTGCAGGCAATAAACATGTGTCGGTGAATTTACCCGTACCCCAGCAAAACGCGGTCAGCCACCGttcgcgttgactgcttat      >  2:166578/1‑235 (MQ=255)

                                                                                                                                                                                                                                                        gggaggggaATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTGACCTGCTAAtt     <  2:642917/251‑1 (MQ=255)

                                                                                                                                                                                                                                                        gggaggggaATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTGACCTGCTAAtt     <  1:103044/251‑1 (MQ=255)

                                                                                                                                                                                                                                                          gaggggaATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATCACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTGACCTGCTAATTGc   <  2:837715/251‑1 (MQ=255)

                                                                                                                                                                                                                                                           aggggaATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTGACCTGCTAATTGCt  >  1:141307/1‑251 (MQ=255)

ACATCCGGTTGTGCTGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGG‑TTGG‑TTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGG‑AAATTCCTCTGGATCAAAAACTCA‑CCCATTACGAACA‑GCTGGCAAGTGATGCACTGGCAGGAG‑AAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCA‑AAA‑TTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTGTTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTGACCTGCTAATTGCT  >  NC_000913/4622001‑4622500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`