breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 3,645 2 bp→AT coding (845‑846/933 nt) thrB → homoserine kinase
RA 71,170 T→C S262P (TCG→CCG)  araC → DNA‑binding transcriptional dual regulator AraC
RA 81,102 G→A S286L (TCG→TTG)  leuB ← 3‑isopropylmalate dehydrogenase
RA 88,756 C→T F243F (TTC→TTT cra → DNA‑binding transcriptional dual regulator Cra
RA 98,060 G→T G325V (GGC→GTC)  murD → UDP‑N‑acetylmuramoyl‑L‑alanine‑‑D‑glutamate ligase
RA 108,958 G→T G227V (GGC→GTC)  secA → protein translocation ATPase
RA 154,182 C→T L415L (CTG→CTA htrE ← putative fimbrial usher protein HtrE
RA 156,068 A→G V45A (GTA→GCA)  yadV ← putative fimbrial chaperone YadV
RA 169,496 Δ1 bp coding (2013/2244 nt) fhuA → ferrichrome outer membrane transporter/phage receptor
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
MC JC 258,999 Δ14,956 bp IS5‑mediated [crl][ykfC] 21 genes
MC 279,931 Δ11,471 bp between IS1 yagBinsA3 yagB, yagA, yagE, yagF, yagG, yagH, xynR, argF, argL, ykgS, insB3, insA3
RA 292,019 C→T L174L (CTA→TTA)  yagJ → protein YagJ
RA 309,381 C→T D216N (GAT→AAT)  ecpB ← putative fimbrial chaperone EcpB
RA 309,985 T→A G14G (GGA→GGT ecpB ← putative fimbrial chaperone EcpB
RA 319,825 C→T Q59Q (CAG→CAA rclA ← cupric reductase RclA
RA 339,048 C→T H242Y (CAT→TAT)  yahG → DUF1116 domain‑containing protein YahG
RA 351,263 G→A V17I (GTT→ATT)  prpD → 2‑methylcitrate dehydratase
JC JC 362,204 IS1 (–) +9 bp coding (968‑976/1254 nt) lacY ← lactose permease
RA 380,909 C→A pseudogene (352/417 nt) yaiX ← putative acyltransferase, N‑terminal fragment
RA 405,292 G→T A118D (GCC→GAC)  proC ← pyrroline‑5‑carboxylate reductase
RA 410,666 C→T P175S (CCT→TCT)  mak → fructokinase
RA 421,027 C→T R14R (CGC→CGT proY → putative transporter ProY
JC JC 431,304 IS1 (–) +9 bp coding (702‑710/885 nt) tsx ← nucleoside‑specific channel‑forming protein Tsx
RA 463,731 G→A S606N (AGT→AAT)  ppiD → periplasmic folding chaperone
RA 472,849 A→G G84G (GGA→GGG glnK → nitrogen regulatory protein PII‑2
RA 495,622 C→T T168I (ACC→ATC)  htpG → chaperone protein HtpG
RA 534,742 G→A G276D (GGT→GAT)  gcl → glyoxylate carboligase
RA 539,897 G→A E251K (GAA→AAA)  allB → allantoinase
RA 552,850 G→T T84K (ACG→AAG)  purE ← N(5)‑carboxyaminoimidazole ribonucleotide mutase
RA 574,255 G→A Q100Q (CAG→CAA quuD → prophage antitermination protein Q homolog QuuD
MC JC 575,786 Δ3,398 bp IS5‑mediated [nmpC]borD [nmpC], essD, rrrD, rzpD, rzoD, borD
RA 582,277 C→T intergenic (+180/‑598) tfaD → / → tfaX putative tail fiber assembly protein TfaD/protein TfaX
RA 582,597 C→T intergenic (+500/‑278) tfaD → / → tfaX putative tail fiber assembly protein TfaD/protein TfaX
RA 620,757 G→A T144T (ACC→ACT fepG ← ferric enterobactin ABC transporter membrane subunit FepG
RA 634,877 C→T G167E (GGA→GAA)  ybdM ← ParB‑like nuclease domain‑containing protein YbdM
RA 635,281 G→A I32I (ATC→ATT ybdM ← ParB‑like nuclease domain‑containing protein YbdM
RA 650,062 C→T G143G (GGG→GGA citE ← citrate lyase beta subunit
RA 658,024 G→T intergenic (‑86/‑923) crcB ← / → tatE F(‑) channel protein/twin arginine protein translocation system ‑ TatE protein
RA 662,857 C→T T369T (ACG→ACA dacA ← D‑alanyl‑D‑alanine carboxypeptidase DacA
RA 667,216 G→T P334P (CCC→CCA mrdA ← peptidoglycan DD‑transpeptidase MrdA
RA 681,755 C→T A547T (GCG→ACG)  hscC ← chaperone protein HscC
RA 694,941 G→A L5L (CTC→CTT miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 769,388 A→T I471F (ATC→TTC)  mngB → alpha‑mannosidase
RA 780,624 C→T noncoding (71/76 nt) lysT → tRNA‑Lys
RA 789,580 C→A E134* (GAA→TAA)  galK ← galactokinase
RA 822,527 C→T E405K (GAA→AAA)  clsB ← cardiolipin synthase B
RA 855,793 A→G intergenic (‑49/‑170) ldtB ← / → ybiT L,D‑transpeptidase LdtB/putative ATP‑binding protein YbiT
RA 883,396 C→T intergenic (‑8/‑277) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 908,169 C→T R38R (AGG→AGA ybjT ← putative NAD(P)‑binding protein YbjT
RA 953,082 A→G L158P (CTG→CCG)  pflB ← pyruvate formate‑lyase
RA 980,038 G→A R1238H (CGC→CAC)  mukB → chromosome partitioning protein MukB
MC JC 1,076,135 Δ80 bp coding (2669‑2748/3963 nt) putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,123,823 2 bp→AG coding (231‑232/648 nt) grxB ← reduced glutaredoxin 2
RA 1,147,792 T→A S58T (TCG→ACG)  plsX → putative phosphate acyltransferase
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,194,922 G→A T10T (ACC→ACT rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,253,002 C→T intergenic (+17/+83) dhaR → / ← ycgV DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
JC JC 1,290,524 IS1 (–) +9 bp coding (283‑291/1014 nt) rssB → regulator of RpoS
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,308,684 T→C intergenic (‑39/‑104) clsA ← / → yciY cardiolipin synthase A/uncharacterized protein YciY
RA 1,322,532 C→A E139* (GAA→TAA)  trpE ← anthranilate synthase subunit TrpE
RA 1,323,819 G→A G200G (GGG→GGA rnm → RNase AM
RA 1,329,565 G→A K78K (AAG→AAA sohB → S49 peptidase family protein
RA 1,331,059 2 bp→AT coding (12‑13/2598 nt) topA → DNA topoisomerase 1
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,349,872 G→A T79I (ACC→ATC)  yciW ← putative peroxidase YciW
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,376,356 G→A R108H (CGC→CAC)  ycjR → 3‑dehydro‑D‑guloside 4‑epimerase
RA 1,381,277 G→A intergenic (+470/‑670) ycjU → / → ompG beta‑phosphoglucomutase/outer membrane porin G
RA 1,381,637 G→A intergenic (+830/‑310) ycjU → / → ompG beta‑phosphoglucomutase/outer membrane porin G
RA 1,397,837 C→A Q262H (CAG→CAT uspE ← universal stress protein E
RA 1,405,758 G→A intergenic (‑7/‑221) mcaS ← / → smrA small regulatory RNA McaS/DNA endonuclease SmrA
RA 1,422,400 G→A V140I (GTT→ATT)  ydaV → putative ATP‑binding protein YdaV
RA 1,423,537 G→A W46* (TGG→TGA rzoR → putative prophage outer membrane lipoprotein RzoR
RA 1,426,810 G→A E119E (GAG→GAA ynaA → putative prophage tail length tape measure domain‑containing protein YnaA
RA 1,432,971 G→A W187* (TGG→TGA tfaR → putative tail fiber assembly protein TfaR
RA 1,435,877 G→A N339N (AAC→AAT ompN ← outer membrane porin N
RA 1,491,485 G→A noncoding (22/65 nt) rydC ← small regulatory RNA RydC
RA 1,497,385 4 bp→CAA coding (530‑533/1656 nt) opgD → glucans biosynthesis protein D
RA 1,558,340 G→A S226S (AGC→AGT ddpD ← putative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpD
JC JC 1,590,536 IS2 (+) +5 bp intergenic (‑535/+314) ydeT ← / ← hipA fimbrial usher domain‑containing protein YdeT/serine/threonine‑protein kinase toxin HipA
RA 1,629,247 C→T I11I (ATC→ATT ydfZ → putative selenoprotein YdfZ
RA 1,638,244 2 bp→AG coding (9‑10/66 nt) ynfR ← protein YnfR
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,658,370 C→T S101F (TCC→TTC)  ynfE → putative selenate reductase YnfE
RA 1,667,460 G→A Q369* (CAG→TAG)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,674,654 C→A P248T (CCT→ACT)  tqsA → autoinducer 2 exporter
RA 1,682,459 A→G K101E (AAA→GAA)  rstA → DNA‑binding transcriptional regulator RstA
RA 1,748,841 C→T R240K (AGA→AAA)  ydhT ← uncharacterized protein YdhT
RA 1,753,888 G→C A197G (GCG→GGG)  ydhY ← putative 4Fe‑4S ferredoxin‑like protein YdhY
RA 1,767,014 C→T G558S (GGT→AGT)  ydiJ ← putative FAD‑linked oxidoreductase YdiJ
RA 1,796,924 G→A L341L (CTG→TTG)  pheT ← phenylalanine‑‑tRNA ligase subunit beta
RA 1,814,677 G→A G271S (GGC→AGC)  katE → catalase HPII
RA 1,829,758 G→A R336R (CGC→CGT astA ← arginine N‑succinyltransferase
RA 1,848,300 2 bp→TT coding (376‑377/552 nt) ydjA ← putative oxidoreductase YdjA
RA 1,849,629 T→C L265L (TTA→CTA)  sppA → protease IV, a signal peptide peptidase
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,931,995 A→G E372G (GAG→GGG)  purT → phosphoribosylglycinamide formyltransferase 2
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,041,278 G→A intergenic (+160/‑97) msrQ → / → zinT membrane‑bound flavocytochrome MsrQ/metal‑binding protein ZinT
RA 2,052,885 G→A intergenic (+871/‑758) yeeJ → / → shiA inverse autotransporter adhesin/shikimate:H(+) symporter
RA 2,095,666 G→C G181A (GGC→GCC)  hisA → 1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase
RA 2,095,818 A→T K232* (AAA→TAA)  hisA → 1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase
RA 2,104,024 G→T I162I (ATC→ATA wbbK ← putative glycosyltransferase WbbK
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,142,598 G→A L117L (CTC→CTT udk ← uridine/cytidine kinase
RA 2,155,197 G→A Q394Q (CAG→CAA mdtA → multidrug efflux pump membrane fusion protein MdtA
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,173,448 C→T intergenic (‑575/+834) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC JC 2,176,844 IS4 (–) +13 bp coding (349‑361/855 nt) gatY ← tagatose‑1,6‑bisphosphate aldolase 2
RA 2,214,092 C→G V185L (GTC→CTC)  btsS ← high‑affinity pyruvate receptor
RA 2,233,609 C→T Q4* (CAG→TAG)  yeiS → DUF2542 domain‑containing protein YeiS
JC JC 2,238,948 Δ1 bp :: IS186 (–) +6 bp :: Δ2 bp coding (337‑342/1521 nt) mglA ← D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit
RA 2,242,247 C→T G241R (GGG→AGG)  yeiB ← DUF418 domain‑containing protein YeiB
RA 2,247,134 A→C Y467D (TAC→GAC)  lysP ← lysine:H(+) symporter
RA 2,268,622 T→A V265E (GTG→GAG)  yeiR → zinc‑binding GTPase YeiR
RA 2,270,181 C→A S68* (TCG→TAG)  mepS → peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
RA 2,270,384 C→T L136L (CTG→TTG)  mepS → peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
RA 2,292,114 C→T P99P (CCG→CCA ccmH ← holocytochrome c synthase CcmH component
RA 2,307,733 C→T A295T (GCG→ACG)  yojI ← ABC transporter family protein/microcin J25 efflux protein
RA 2,310,878 2 bp→TT coding (656‑657/1056 nt) ftp ← FAD:protein FMN transferase
RA 2,323,636 C→T L64F (CTC→TTC)  atoD → acetyl‑CoA:acetoacetyl‑CoA transferase subunit alpha
RA 2,404,402 G→A S71L (TCG→TTG)  nuoB ← NADH:quinone oxidoreductase subunit B
RA 2,446,592 C→A P294P (CCG→CCT aroC ← chorismate synthase
RA 2,455,977 G→A intergenic (‑331/+350) yfcV ← / ← sixA putative fimbrial protein YfcV/phosphohistidine phosphatase SixA
RA 2,468,250 G→A E13K (GAA→AAA)  yfdH → bactoprenol glucosyl transferase
RA 2,483,558 G→A intergenic (‑219/‑171) emrK ← / → evgL tripartite efflux pump membrane fusion protein EmrK/protein EvgL
RA 2,485,636 Δ1 bp coding (1263/3594 nt) evgS → sensor histidine kinase EvgS
RA 2,485,638 T→A L422* (TTA→TAA)  evgS → sensor histidine kinase EvgS
RA 2,522,369 G→A P37S (CCG→TCG)  xapR ← DNA‑binding transcriptional activator XapR
RA 2,535,775 A→C E570D (GAA→GAC ptsI → PTS enzyme I
RA 2,558,170 C→T V170V (GTG→GTA eutB ← ethanolamine ammonia‑lyase subunit alpha
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
RA 2,637,757 A→C V299G (GTC→GGC)  bamB ← outer membrane protein assembly factor BamB
RA 2,693,388 G→A A719V (GCG→GTG)  purL ← phosphoribosylformylglycinamide synthetase
RA 2,702,085 G→A P129S (CCC→TCC)  recO ← recombination mediator protein RecO
RA 2,702,363 G→A A36V (GCC→GTC)  recO ← recombination mediator protein RecO
RA 2,703,619 G→A P149S (CCA→TCA)  rnc ← RNase III
RA 2,707,495 C→T D103N (GAC→AAC)  rseC ← protein RseC
RA 2,709,220 G→A A62V (GCC→GTC)  rseA ← anti‑sigma‑E factor RseA
RA 2,737,133 G→A intergenic (+250/‑21) bamD → / → raiA outer membrane protein assembly factor BamD/ribosome‑associated inhibitor A
RA 2,737,590 G→A intergenic (+48/‑9) raiZ → / → pheL small RNA RaiZ/phe operon leader peptide
RA 2,748,741 G→A Q260Q (CAG→CAA ypjD → cytochrome c assembly family protein
RA 2,769,594 G→A intergenic (+108/‑109) yfjQ → / → yfjR DUF932 domain‑containing protein YfjQ/putative DNA‑binding transcriptional regulator YfjR
RA 2,774,947 G→A G544S (GGT→AGT)  yfjW → uncharacterized protein YfjW
RA 2,803,859 G→A A120T (GCC→ACC)  nrdF → ribonucleoside‑diphosphate reductase 2 subunit beta
RA 2,810,385 G→A V14V (GTG→GTA ygaH → L‑valine exporter, YgaH component
RA 2,818,668 G→A intergenic (‑23/+293) serV ← / ← csrA tRNA‑Ser/carbon storage regulator
RA 2,826,108 G→A E93K (GAA→AAA)  srlA → sorbitol‑specific PTS enzyme IIC2 component
RA 2,838,419 G→A P282P (CCC→CCT ascG ← DNA‑binding transcriptional repressor AscG
RA 2,856,383 G→A K682K (AAG→AAA fhlA → DNA‑binding transcriptional activator FhlA
RA 2,857,187 G→A R32Q (CGG→CAG)  mutS → DNA mismatch repair protein MutS
RA 2,867,311 G→T R81S (CGT→AGT)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,880,635 T→C L138L (TTA→TTG casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,892,535 G→A V108I (GTA→ATA)  queD → 6‑carboxy‑5,6,7,8‑tetrahydropterin synthase
RA 2,894,076 G→A S53N (AGT→AAT)  ygcO → putative 4Fe‑4S cluster‑containing protein
RA 2,902,376 G→A A161T (GCT→ACT)  ygcE → putative sugar kinase YgcE
RA 2,906,563 G→A G285S (GGC→AGC)  ygcG → TPM domain‑containing protein YgcG
RA 2,906,909 G→A Q345* (CAG→TAG)  eno ← enolase
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 2,928,947 G→A G240D (GGC→GAC)  sdaC → L‑serine:H(+) symporter SdaC
RA 2,936,855 T→G A424A (GCT→GCG fucI → L‑fucose isomerase
RA 2,937,210 C→T L543F (CTC→TTC)  fucI → L‑fucose isomerase
RA 2,938,548 A→T T361S (ACG→TCG)  fucK → L‑fuculokinase
RA 2,945,282 G→A S187S (AGC→AGT tcdA ← tRNA threonylcarbamoyladenosine dehydratase
RA 2,946,310 G→A T290I (ACC→ATC)  mltA ← membrane‑bound lytic murein transglycosylase A
RA 2,954,917 G→A R363C (CGT→TGT)  recB ← exodeoxyribonuclease V subunit RecB
RA 2,961,324 G→A P369S (CCA→TCA)  recC ← exodeoxyribonuclease V subunit RecC
RA 2,961,438 G→A P331S (CCA→TCA)  recC ← exodeoxyribonuclease V subunit RecC
RA 2,986,809 G→A Q321Q (CAG→CAA yqeG → putative transporter YqeG
RA 3,010,818 G→A C264Y (TGC→TAC)  hyuA → phenylhydantoinase
RA 3,018,087 G→A K676K (AAG→AAA ygfK → putative oxidoreductase, Fe‑S subunit
RA 3,025,601 (T)5→4 coding (1251/1401 nt) xanQ → xanthine:H(+) symporter XanQ
RA 3,070,858 T→G K129N (AAA→AAC fbaA ← fructose‑bisphosphate aldolase class II
RA 3,080,777 G→T P287T (CCG→ACG)  tktA ← transketolase 1
RA 3,089,059 A→T Q259L (CAG→CTG)  galP → galactose:H(+) symporter
RA 3,179,064 G→A G317D (GGC→GAC)  tolC → outer membrane channel TolC
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,227,963 G→C G55R (GGG→CGG)  ygjJ → protein YgjJ
RA 3,274,864 G→A T15I (ACC→ATC)  garP ← galactarate/D‑glucarate transporter GarP
RA 3,279,276 C→G F121L (TTC→TTG kbaZ → putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone
RA 3,296,481 G→A V25M (GTG→ATG)  dolP → division and outer membrane stress‑associated lipid‑binding lipoprotein
RA 3,319,301 Δ1 bp coding (665/1344 nt) argG → argininosuccinate synthetase
RA 3,324,965 G→T intergenic (‑54/+36) folP ← / ← ftsH dihydropteroate synthase/ATP‑dependent zinc metalloprotease FtsH
RA 3,326,537 C→A S133S (TCG→TCT ftsH ← ATP‑dependent zinc metalloprotease FtsH
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,411,689 G→T E13* (GAA→TAA)  yhdJ → DNA adenine methyltransferase
RA 3,433,900 C→T L71L (CTA→TTA)  def → peptide deformylase
RA 3,444,904 C→T G109D (GGT→GAT)  rpsE ← 30S ribosomal subunit protein S5
RA 3,446,191 C→T A119T (GCT→ACT)  rpsH ← 30S ribosomal subunit protein S8
RA 3,452,079 A→T S70T (TCT→ACT)  rplD ← 50S ribosomal subunit protein L4
RA 3,474,424 T→G K43N (AAA→AAC rpsL ← 30S ribosomal subunit protein S12
RA 3,486,502 C→T T128I (ACT→ATT)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,486,652 G→C G178A (GGC→GCC)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,491,631 C→A intergenic (‑11/+94) yhfG ← / ← ppiA DUF2559 domain‑containing protein YhfG/peptidyl‑prolyl cis‑trans isomerase A
RA 3,511,466 C→T V293V (GTG→GTA yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,555,047 G→C V655L (GTC→CTC)  malT → DNA‑binding transcriptional activator MalT
MC JC 3,555,171 Δ11 bp coding (2087‑2097/2706 nt) malT → DNA‑binding transcriptional activator MalT
RA 3,560,362 G→A intergenic (+496/+260) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,646,287 G→A intergenic (+60/‑12) rlmJ → / → gor 23S rRNA m(6)A2030 methyltransferase/glutathione reductase (NADPH)
RA 3,647,428 C→T A377V (GCG→GTG)  gor → glutathione reductase (NADPH)
RA 3,653,066 G→A P33S (CCA→TCA)  insH11 ← IS5 family transposase and trans‑activator
RA 3,661,226 G→A G271G (GGG→GGA mdtF → multidrug efflux pump RND permease MdtF
RA 3,663,006 G→A A865T (GCG→ACG)  mdtF → multidrug efflux pump RND permease MdtF
RA 3,664,928 G→A noncoding (65/106 nt) gadY → small regulatory RNA GadY
RA 3,688,359 G→A A272V (GCT→GTT)  bcsC ← cellulose synthase outer membrane channel
RA 3,689,787 G→A L159L (CTG→TTG)  bcsZ ← endo‑1,4‑D‑glucanase
RA 3,692,266 G→A N114N (AAC→AAT bcsB ← cellulose synthase periplasmic subunit
RA 3,693,241 G→A L666F (CTC→TTC)  bcsA ← cellulose synthase catalytic subunit
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,715,926 G→A P157S (CCG→TCG)  bisC ← biotin sulfoxide reductase
RA 3,719,429 G→A intergenic (‑385/‑49) yiaF ← / → yiaG DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG
RA 3,720,794 G→A V39I (GTC→ATC)  insJ → insertion element IS150 protein InsA
RA 3,724,217 G→A L61L (CTG→TTG)  glyS ← glycine‑‑tRNA ligase subunit beta
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,729,440 2 bp→TT noncoding (105‑106/160 nt) xylZ ← small RNA XylZ
RA 3,730,083 G→A T228M (ACG→ATG)  xylA ← xylose isomerase
RA 3,730,560 C→T W69* (TGG→TAG)  xylA ← xylose isomerase
RA 3,739,696 T→G intergenic (+169/‑9) malS → / → avtA alpha‑amylase/valine‑‑pyruvate aminotransferase
RA 3,746,267 G→A E58E (GAG→GAA yiaO → 2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ periplasmic binding protein
RA 3,760,259 G→A L331F (CTC→TTC)  selA ← selenocysteine synthase
RA 3,770,539 C→T V281M (GTG→ATG)  yibH ← inner membrane protein YibH
RA 3,771,188 G→A I64I (ATC→ATT yibH ← inner membrane protein YibH
RA 3,773,173 Δ1 bp coding (893/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,773,176 C→T S299F (TCT→TTT)  mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,776,302 C→T E27K (GAG→AAG)  yibT ← protein YibT
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,839,888 C→T V36I (GTT→ATT)  nlpA ← lipoprotein‑28
RA 3,858,402 C→T M1M (ATG→ATA) †
*572* (TGA→TAA) 
yidJ ←
yidK ←
putative sulfatase/phosphatase YidJ
putative transporter YidK
RA 3,914,237 C→T A322T (GCG→ACG)  glmU ← fused N‑acetylglucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyltransferase
RA 3,926,991 G→A L5L (CTG→TTG)  asnC ← DNA‑binding transcriptional dual regulator AsnC
RA 3,936,392 C→T P39S (CCG→TCG)  rbsB → ribose ABC transporter periplasmic binding protein
RA 3,952,581 G→A G33D (GGC→GAC)  ilvE → branched‑chain‑amino‑acid aminotransferase
RA 3,956,495 C→T L389F (CTC→TTC)  ilvA → threonine deaminase
RA 3,966,530 2 bp→TT coding (114‑115/1260 nt) rho → transcription termination factor Rho
RA 3,969,914 G→A G295D (GGT→GAT)  wzzE → enterobacterial common antigen polysaccharide co‑polymerase
RA 3,979,828 G→A V410I (GTC→ATC)  wzyE → enterobacterial common antigen polymerase
RA 3,982,060 G→A A392T (GCG→ACG)  yifK → putative transporter YifK
RA 3,993,808 G→A G84G (GGC→GGT cyaY ← frataxin CyaY
RA 3,993,970 G→A I30I (ATC→ATT
D41N (GAT→AAT) 
cyaY ←
yzcX →
frataxin CyaY
protein YzcX
RA 4,003,502 G→T P226Q (CCG→CAG)  rarD ← putative transporter RarD
RA 4,028,198 G→A T192I (ACC→ATC)  fadA ← 3‑ketoacyl‑CoA thiolase
RA 4,028,589 G→A L62L (CTG→TTG)  fadA ← 3‑ketoacyl‑CoA thiolase
RA 4,036,965 G→A noncoding (1435/1542 nt) rrsA → 16S ribosomal RNA
RA 4,042,296 C→T F76F (TTC→TTT yihD → DUF1040 domain‑containing protein YihD
RA 4,057,018 G→A N339N (AAC→AAT glnA ← glutamine synthetase
RA 4,068,524 G→A D251D (GAC→GAT yihQ ← sulfoquinovosidase
RA 4,072,919 G→A T218I (ACT→ATT)  yihU ← 3‑sulfolactaldehyde reductase
RA 4,074,387 G→A G217S (GGC→AGC)  yihV → 6‑deoxy‑6‑sulfofructose kinase
RA 4,077,794 G→A E116K (GAA→AAA)  fabY → putative fatty acid biosynthesis enzyme FabY
RA 4,080,171 G→A intergenic (+202/+128) yiiF → / ← fdhE protein YiiF/formate dehydrogenase formation protein
RA 4,100,202 G→A G108G (GGC→GGT rhaT ← rhamnose/lyxose:H(+) symporter
RA 4,101,503 G→A intergenic (+73/‑187) sodA → / → kdgT superoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
RA 4,102,164 G→A G159S (GGC→AGC)  kdgT → 2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
RA 4,110,704 G→A intergenic (+19/+36) cdh → / ← tpiA CDP‑diacylglycerol diphosphatase/triose‑phosphate isomerase
RA 4,111,879 Δ1 bp coding (336/600 nt) yiiQ ← DUF1454 domain‑containing protein YiiQ
RA 4,119,232 G→A G33G (GGC→GGT rraA ← ribonuclease E inhibitor protein A
JC JC 4,123,617 IS2 (–) +5 bp coding (836‑840/1026 nt) cytR ← DNA‑binding transcriptional repressor CytR
RA 4,132,781 G→A V56I (GTA→ATA)  metF → 5,10‑methylenetetrahydrofolate reductase
RA 4,134,190 G→A G119D (GGC→GAC)  katG → catalase/hydroperoxidase HPI
RA 4,150,418 A→G intergenic (‑153/+29) eptC ← / ← ppc phosphoethanolamine transferase EptC/phosphoenolpyruvate carboxylase
RA 4,174,944 G→A P28L (CCT→CTT)  coaA ← pantothenate kinase
RA 4,184,802 G→A V1186V (GTG→GTA rpoB → RNA polymerase subunit beta
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,199,100 G→A G104D (GGT→GAT)  nfi → endonuclease V
RA 4,203,291 G→A V456I (GTC→ATC)  zraS → sensor histidine kinase ZraS
RA 4,206,667 G→A A289V (GCC→GTC)  purH ← bifunctional AICAR transformylase/IMP cyclohydrolase
RA 4,214,979 G→T G234C (GGC→TGT)  metA → homoserine O‑succinyltransferase
RA 4,214,981 C→T G234C (GGC→TGT metA → homoserine O‑succinyltransferase
RA 4,217,310 A→G S68G (AGC→GGC)  aceA → isocitrate lyase
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,227,299 G→A E1158K (GAA→AAA)  metH → cobalamin‑dependent methionine synthase
RA 4,229,149 G→A L473L (TTG→TTA yjbB → putative inorganic phosphate export protein YjbB
RA 4,234,916 G→A A387T (GCG→ACG)  pgi → glucose‑6‑phosphate isomerase
RA 4,245,867 G→A P185S (CCG→TCG)  malE ← maltose ABC transporter periplasmic binding protein
RA 4,246,347 G→A L25F (CTC→TTC)  malE ← maltose ABC transporter periplasmic binding protein
RA 4,253,905 G→A intergenic (+17/+138) ubiA → / ← plsB 4‑hydroxybenzoate octaprenyltransferase/glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,799 G→A S223F (TCC→TTC)  plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,264,969 C→G A219G (GCT→GGT)  dnaB → replicative DNA helicase
RA 4,265,653 G→A G447E (GGG→GAG)  dnaB → replicative DNA helicase
RA 4,269,646 G→A S78N (AGT→AAT)  aphA → acid phosphatase/phosphotransferase
RA 4,272,615 G→A R419R (CGC→CGT uvrA ← UvrABC excision nuclease subunit A
RA 4,273,696 G→A A59V (GCC→GTC)  uvrA ← UvrABC excision nuclease subunit A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,299,919 G→A A371V (GCC→GTC)  mdtP ← putative multidrug efflux pump outer membrane channel
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,331,890 C→T L463L (CTC→CTT proP → osmolyte:H(+) symporter ProP
RA 4,352,154 G→A V24I (GTC→ATC)  yjdJ → putative N‑acetyltransferase YjdJ
RA 4,359,223 G→A A270V (GCT→GTT)  cadB ← lysine:cadaverine antiporter
RA 4,359,371 G→A P221S (CCG→TCG)  cadB ← lysine:cadaverine antiporter
RA 4,363,999 G→A N348N (AAC→AAT dsbD ← thiol‑disulfide exchange protein DsbD
RA 4,364,490 G→A P185S (CCA→TCA)  dsbD ← thiol‑disulfide exchange protein DsbD
RA 4,397,469 G→A V20I (GTC→ATC)  mutL → DNA mismatch repair protein MutL
RA 4,406,983 C→T L110L (CTC→CTT rnr → RNase R
RA 4,422,619 G→A G145D (GGC→GAC)  ulaD → 3‑keto‑L‑gulonate‑6‑phosphate decarboxylase UlaD
RA 4,434,584 G→A intergenic (+91/+38) ytfH → / ← cpdB putative transcriptional regulator YtfH/2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase/3'‑nucleotidase
RA 4,437,890 C→T Q62* (CAG→TAG)  ytfI → protein YtfI
RA 4,444,331 G→A A74T (GCG→ACG)  tamB → translocation and assembly module subunit TamB
RA 4,453,174 G→A G202R (GGG→AGG)  ytfT → galactofuranose ABC transporter putative membrane subunit YtfT
RA 4,454,078 G→A G166R (GGA→AGA)  yjfF → galactofuranose ABC transporter putative membrane subunit YjtF
RA 4,455,185 G→A T142M (ACG→ATG)  fbp ← fructose‑1,6‑bisphosphatase 1
RA 4,459,014 G→A R352R (CGG→CGA tldE → metalloprotease subunit TldE
RA 4,462,727 G→A intergenic (‑67/+327) nrdD ← / ← treC anaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑phosphate hydrolase
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,474,021 G→A I11I (ATC→ATT bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,474,056 G→A intergenic (‑3/‑68) bdcA ← / → bdcR c‑di‑GMP‑binding biofilm dispersal mediator protein/putative transcriptional regulator BdcR
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,476,605 G→A S390N (AGC→AAC)  yjgL → protein YjgL
RA 4,489,016 C→T E350K (GAA→AAA)  yjgR ← DUF853 domain‑containing protein YjgR
RA 4,494,603 G→A intergenic (‑197/‑20) idnD ← / → idnK L‑idonate 5‑dehydrogenase/D‑gluconate kinase, thermosensitive
RA 4,499,294 G→A E234K (GAG→AAG)  insD6 → IS2 insertion element protein InsB
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,513,267 G→A T48M (ACG→ATG)  fecC ← ferric citrate ABC transporter membrane subunit FecC
RA 4,518,062 G→A I58I (ATC→ATT fecI ← RNA polymerase sigma factor FecI
RA 4,530,332 G→A L68F (CTT→TTT)  sgcB ← putative PTS enzyme IIB component SgcB
JC JC 4,542,042 IS1 (+) +8 bp coding (6‑13/597 nt) fimE → regulator for fimA
RA 4,543,847 G→A A40A (GCG→GCA fimI → putative fimbrial protein FimI
RA 4,545,091 G→A intergenic (+62/‑5) fimC → / → fimD type 1 fimbriae periplasmic chaperone/type I fimbriae usher protein
RA 4,545,646 C→T A184V (GCC→GTC)  fimD → type I fimbriae usher protein
RA 4,550,901 G→A A132A (GCC→GCT gntP ← fructuronate transporter
RA 4,554,876 G→A A101T (GCG→ACG)  uxuR → DNA‑binding transcriptional repressor UxuR
RA 4,608,611 Δ1 bp coding (427/447 nt) rimI → protein N‑acetyltransferase RimI

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196245 1211460 15216 42 [40] [41] 47 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 3423748–3424533 3424533 1–786 42 [41] [41] 42 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4542682 =92 (0.840)83 (0.780) 54/580 0.8 51.5% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 67 (0.630)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 4542690100 (0.910)92 (0.870) 62/580 0.5 52.9% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 67 (0.630)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit