Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,432,971	G→A	W187* (TGG→TGA)	tfaR →	putative tail fiber assembly protein TfaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,432,971	0	G	A	100.0%	87.5 / NA	45	W187* (TGG→TGA)	tfaR	putative tail fiber assembly protein TfaR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/15);  total (30/15)

AAAAGTATCGGGTATTGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGGCCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTAC  >  NC_000913/1432913‑1433266                                                           |                                                                                                                                                                                                                                                                                                        aaaaGTATCGGGTATTGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATNTGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACNTAATTCGTTCGAATACCACCTGTTTCTGttc                                                        <  2:620580/300‑1 (MQ=14)   aaGTATCGGGTATTGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGttctt                                                      >  1:343423/1‑300 (MQ=14)    aGTATCGGGTATTGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGAACAGCACTGCCGTAGGGTAACACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACAGAGATTCTTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCCATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGttcttc                                                     >  1:346738/1‑300 (MQ=9)          gggTATTGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTaa                                               >  2:288607/1‑300 (MQ=14)           tgTATTGCTGGACCGTGTTGATACGTCAACTGCACAGGGTGTTGAACGACCAGCACTGCCTTATGGTAAAACATATAAATCCTAGAATTAGATGTATCTTTCCATTTACGTCAAGTAACTGTGCTTTTAAGGCGTAAGGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAac                                              <  2:235885/299‑1 (MQ=11)           ggTATTGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAac                                              <  1:487648/300‑1 (MQ=14)             tatttcttaaCCGTGTTGGTACGTCAGCTGCACAGGATATTAAATGACCAGCACTGCCGTATGGTAAAACATATAAATTCTATAATTGGATGTATCTTTCCATTGACGGCAAGGAAGTGGGCTTTGAAGACGTGAGGCGGCGAACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAacac                                            <  2:330405/292‑1 (MQ=11)             tATTGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGNAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAacac                                            <  1:600520/300‑1 (MQ=14)             tATTGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAacac                                            <  1:26928/300‑1 (MQ=14)               tttcTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCAGTAGGGTAACACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCNCTTAAAATGGTTTGCCGAGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACag                                          >  1:599681/4‑300 (MQ=9)               ttGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATACTTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCANGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCAGCGAGGTTTTGAATTCCCAGGCAATGGCACTTATACTTACACATGACTTAATTCGTTCGACTATCACCTGTTTCTGTTTTTCATTTAacaaag                                          >  2:212086/1‑297 (MQ=11)               ttGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGNAGACGTAAAGCATCTCACACCGAGATNATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACag                                          >  2:578998/1‑300 (MQ=14)               ttGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTNAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACag                                          >  2:748170/1‑300 (MQ=14)               ttGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGCATACCACCTGTTTCTGTTTTTCATTTAAAACag                                          >  2:834531/1‑300 (MQ=9)               ttGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACag                                          >  1:771555/1‑300 (MQ=14)               ttGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCCTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCACGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGCATTAATTCGTTTGAATACCACCTGTTTCTGTTCTTCATTTAACACag                                          >  2:50771/1‑300 (MQ=9)               ttGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGAACAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGACGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAACGGTTTGCCGCGAGGTTTTGAACTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATATCACCTGTTTCTGTTTTTCATTTAACACag                                          >  2:74737/1‑300 (MQ=255)                tGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCTTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACagg                                         <  2:5475/300‑1 (MQ=14)                tGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACagg                                         <  1:109509/300‑1 (MQ=14)                cgCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAACTCTATAATTAGATGTATCTTTCCATTTACGNCAAGTAAGGGGGCTTGGAAGACGTAAAGCATCGCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACagg                                         <  2:757472/299‑1 (MQ=9)                  cTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGNTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACNACCTGTTTCTGTTCTTCATTTAACACaggag                                       <  2:630523/300‑1 (MQ=17)                    gccccGTGTTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCGGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGt                                     >  2:818223/4‑300 (MQ=17)                         gtgtTGATACGTCAACTGCACAGGATATTGANTGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAANCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGAcc                                >  2:615424/1‑300 (MQ=17)                         gtgtTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAANCATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTNAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGAcc                                >  2:809959/1‑300 (MQ=17)                         gtgtTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAANCATCTCACACCGAGATTATTTTTTATNTGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGAcc                                >  2:577149/1‑300 (MQ=17)                         gtgtTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTCAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACTACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGAcc                                >  2:428104/1‑300 (MQ=17)                         gtgtTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCCTTTAACACAGGAGGTCGAcc                                >  1:29036/1‑300 (MQ=17)                         gtgtTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGAcc                                >  1:14008/1‑300 (MQ=17)                         gtgtTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTAACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGCATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGAcc                                >  2:731671/1‑300 (MQ=17)                         gtgtTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTAACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGAcc                                >  1:328647/1‑300 (MQ=17)                          tgtTGATACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCa                               >  1:570014/1‑300 (MQ=17)                                tACGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTCTACTTACACCTGACTTACTTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGt                         >  2:39182/1‑300 (MQ=17)                                  cGTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCNAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGttt                       >  1:621501/1‑300 (MQ=17)                                   gTCACCTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTCAAACATATAAATTCTATAATTCGATGTATCTTTCCATTTACGGAAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTAACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTCAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTCACACAGGAGGTCGACCAAAACGTTTc                      >  1:780245/1‑300 (MQ=11)                                   gTCAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGNTGTCTGAAGTTTTGCTTTGGCTCTTANAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTc                      <  2:577638/300‑1 (MQ=17)                                     cAACTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTCAATCCGTTCGACTACCACCTATTTCCGTTCTTCATTTAACACAGGAAGTCGACCAAAACGTTTcca                    >  2:104820/1‑299 (MQ=255)                                        cTGCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTgc                 >  1:287497/1‑300 (MQ=17)                                          gCACAGGATCTTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTCTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCATTTATCCCTACACCTCACTTAATTCGTTCGAATACAACCTGTTTCCGTTTTTCATTTCACACAGGGGGTCGACCAAAACGTTTACCTgcgc               >  2:145220/1‑300 (MQ=11)                                          gCACAGGATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTgcgc               <  1:73684/300‑1 (MQ=17)                                               ggATATTGAATGACCAGCATTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACTGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCgg          <  2:362483/300‑1 (MQ=17)                                               ggATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTNCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCgg          >  1:589921/1‑300 (MQ=17)                                               ggATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCgg          >  1:398750/1‑300 (MQ=17)                                               ggATATTGAATGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGAACAAAACGTTTCCCTGCGCCGCgg          >  2:342298/1‑300 (MQ=17)                                                       aaTGACCAGCACTGTCGTAGGGGAAAACATACAAACTCTATAATTAGATGTATCTTTCCATTTACTGCAAGTAATGGTGCTTGGAATCCGTAACGCGTCTACCACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTAc  <  2:279243/300‑1 (MQ=11)                                                       aaTGACCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTAc  <  1:144863/300‑1 (MQ=17)                                                           |                                                                                                                                                                                                                                                                                                        AAAAGTATCGGGTATTGCTGAACCGTGTTGATACGTCAACTGCACAGGATATTGAATGGCCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTAC  >  NC_000913/1432913‑1433266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑