Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,102,164	G→A	G159S (GGC→AGC)	kdgT →	2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,102,164	0	G	A	100.0%	336.1 / NA	94	G159S (GGC→AGC)	kdgT	2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/54);  total (40/54)

CAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGGGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGTTGATTATCGCCGATAAATTGATTGGCGGTGGCGATGGCACTGCCGGAA  >  NC_000913/4101871‑4102458                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                       cAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTg                                                                                                                                                                                                                                                                                                  >  1:436291/1‑300 (MQ=255)       aaTTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGa                                                                                                                                                                                                                                                                                            <  1:319814/300‑1 (MQ=255)                      gAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCTGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAAc                                                                                                                                                                                                                                                                             <  1:69998/300‑1 (MQ=255)                      gAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCTTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAAc                                                                                                                                                                                                                                                                             >  2:141484/1‑300 (MQ=255)                      gAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAAc                                                                                                                                                                                                                                                                             >  1:147123/1‑300 (MQ=255)                      gAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGGTTGAAc                                                                                                                                                                                                                                                                             >  2:742449/1‑300 (MQ=255)                      gAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTACTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCCGGGGCATTCGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATAATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAAc                                                                                                                                                                                                                                                                             >  2:142092/1‑300 (MQ=255)                        aCGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCg                                                                                                                                                                                                                                                                           >  1:120333/1‑300 (MQ=255)                           gTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTNCGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCAt                                                                                                                                                                                                                                                                        >  1:623758/1‑300 (MQ=255)                           gTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCAt                                                                                                                                                                                                                                                                        >  1:30973/1‑300 (MQ=255)                                gCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAANAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGtttt                                                                                                                                                                                                                                                                   >  2:767019/1‑300 (MQ=255)                                                 tggtggNAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTANTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTAc                                                                                                                                                                                                                                                  <  2:709417/300‑1 (MQ=255)                                                   gtggtAACTAAAATTGCCGTCGCGTGGTTGGTTGCTGCAATTGCCTCGCGTATTAGTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATGTGTGTCGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCg                                                                                                                                                                                                                                                <  2:146404/300‑1 (MQ=255)                                                   gtggtAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCTGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCg                                                                                                                                                                                                                                                <  1:78991/300‑1 (MQ=255)                                                   gtggtAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCg                                                                                                                                                                                                                                                <  1:494418/300‑1 (MQ=255)                                                        aaCTAAAATTGCCGTCGCGTGGGTGGTTGTGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCAGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGCGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGCTTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTcat                                                                                                                                                                                                                                           >  2:403556/1‑298 (MQ=255)                                                        aaCTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGNCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACNGTTCCt                                                                                                                                                                                                                                           >  2:704109/1‑300 (MQ=255)                                                        aaCTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCAGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTccc                                                                                                                                                                                                                                           >  2:403685/1‑299 (MQ=255)                                                            aaaaTTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCtggtg                                                                                                                                                                                                                                       >  1:243252/1‑300 (MQ=255)                                                                      tCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGccc                                                                                                                                                                                                                             <  1:248236/300‑1 (MQ=255)                                                                            gggtggTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGa                                                                                                                                                                                                                       >  1:185726/1‑300 (MQ=255)                                                                                gtttGCGGCAATTGCCTCGCGTATTATTCCGGAACATTGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCt                                                                                                                                                                                                                   <  2:771792/298‑1 (MQ=255)                                                                                ggtTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGCTGGCGGCGATGGATATAACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTTAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCt                                                                                                                                                                                                                   <  2:837687/300‑1 (MQ=255)                                                                                 gtTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCtt                                                                                                                                                                                                                  >  2:343624/1‑300 (MQ=255)                                                                                      ggCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTTAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGAccc                                                                                                                                                                                                             <  1:759029/300‑1 (MQ=255)                                                                                           ttGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAAt                                                                                                                                                                                                        <  1:459485/300‑1 (MQ=255)                                                                                                  gcgTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCgaga                                                                                                                                                                                                 >  2:832823/1‑300 (MQ=255)                                                                                                  gcgTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGCATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCgaga                                                                                                                                                                                                 >  2:832847/1‑300 (MQ=255)                                                                                                               gAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCaaa                                                                                                                                                                                    >  2:36811/1‑300 (MQ=255)                                                                                                                 aCATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGc                                                                                                                                                                                  >  1:116472/1‑300 (MQ=255)                                                                                                                      gtgtTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCNTTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGNGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCATGAATTGCGAGAATTTTTCAGCAAAGCGGTGc                                                                                                                                                                             >  2:684656/1‑300 (MQ=255)                                                                                                                      gtgtTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGc                                                                                                                                                                             >  1:256001/1‑300 (MQ=255)                                                                                                                            aaGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGc                                                                                                                                                                       <  1:441426/300‑1 (MQ=255)                                                                                                                             atttGGATTCTTTGCCAGACTTTCAACGCTGGCGCTGGTGTCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATTCAGCAGTACGGCACAAAAGAAGAAGCTGNGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTNCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCt                                                                                                                                                                      <  2:695912/298‑1 (MQ=255)                                                                                                                                  gATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCANTGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGNGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTc                                                                                                                                                                 <  2:791741/300‑1 (MQ=255)                                                                                                                                  gATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGTGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGAtcc                                                                                                                                                                 <  1:509381/300‑3 (MQ=255)                                                                                                                                             ggACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGcc                                                                                                                                                      <  1:201697/300‑1 (MQ=255)                                                                                                                                                      aCGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTg                                                                                                                                             >  1:131819/1‑300 (MQ=255)                                                                                                                                                              gctggTNGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCANCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATAc                                                                                                                                     <  2:710465/300‑1 (MQ=255)                                                                                                                                                              gctggTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATAc                                                                                                                                     <  1:354191/300‑1 (MQ=255)                                                                                                                                                                          gATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGAc                                                                                                                         <  1:84352/300‑1 (MQ=255)                                                                                                                                                                            tGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACtg                                                                                                                       <  1:564992/300‑1 (MQ=255)                                                                                                                                                                             ggATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCNCTGCCGGGATTGCCTCGTTTGAACCGNATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACtgt                                                                                                                      >  2:583029/1‑300 (MQ=255)                                                                                                                                                                                   gACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCTTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGc                                                                                                                >  1:53106/1‑300 (MQ=255)                                                                                                                                                                                     ccAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCATTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGcca                                                                                                              >  2:373621/1‑299 (MQ=255)                                                                                                                                                                                         ttgctgACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAATAAGAAGCTTGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGTGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGAc                                                                                                          <  2:309714/295‑1 (MQ=255)                                                                                                                                                                                               aCTTTANGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTNGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGttt                                                                                                    <  2:712410/300‑1 (MQ=255)                                                                                                                                                                                               aCTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGNGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTNCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGttt                                                                                                    <  2:683178/300‑1 (MQ=255)                                                                                                                                                                                               aCTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGttt                                                                                                    <  2:556408/300‑1 (MQ=255)                                                                                                                                                                                                   tACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTg                                                                                                <  1:373506/300‑1 (MQ=255)                                                                                                                                                                                                        ttCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGAt                                                                                           <  1:751078/300‑1 (MQ=255)                                                                                                                                                                                                         tCCATCATGCAGCAGTACGACACAAAATAAGAAGCTGGTGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATTTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTTCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATc                                                                                          <  2:438943/300‑1 (MQ=255)                                                                                                                                                                                                          ccATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATcc                                                                                         <  1:483824/300‑1 (MQ=255)                                                                                                                                                                                                             tcatGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTTAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGt                                                                                      >  1:420275/1‑300 (MQ=255)                                                                                                                                                                                                               atGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTg                                                                                    <  1:813952/300‑1 (MQ=255)                                                                                                                                                                                                               atGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTAAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGCTTGCCCAGACTGGTTTGCTGGGGATCCTGTTg                                                                                    >  2:469665/1‑300 (MQ=255)                                                                                                                                                                                                               atGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGAACCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTg                                                                                    >  2:517011/1‑300 (MQ=255)                                                                                                                                                                                                               atGCAGCAGTACGGCACAAAAGAAAAAGCGGGGGCATTTGTGTTAATGTCGTTGGAGTCCGGGCCGCTCATTACGATGATTATTCTGAGCACTGCCTGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTg                                                                                    <  2:63950/300‑1 (MQ=255)                                                                                                                                                                                                                 gcagcaGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTggg                                                                                  <  1:563360/300‑1 (MQ=255)                                                                                                                                                                                                                 gcagcaGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTACTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTggg                                                                                  <  1:563290/300‑1 (MQ=255)                                                                                                                                                                                                                  cagcagTACGGCACAAAAGAAGAAGCTTGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGgt                                                                                 <  2:323002/300‑1 (MQ=255)                                                                                                                                                                                                                  cagcagTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGgt                                                                                 >  1:683684/1‑300 (MQ=255)                                                                                                                                                                                                                    gcagTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATANCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGgtgt                                                                               >  2:648654/1‑300 (MQ=255)                                                                                                                                                                                                                       gTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGc                                                                            >  2:655544/1‑300 (MQ=255)                                                                                                                                                                                                                         aCGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCNGCTCATGACGATGATTATTCTGAGCACGGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAg                                                                          <  2:651845/300‑1 (MQ=255)                                                                                                                                                                                                                         aCGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCNGCTCATGACGATGATTATTCTGAGCACCGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAg                                                                          <  2:652243/300‑1 (MQ=255)                                                                                                                                                                                                                             cacaAAAGAAGAAGCTGTGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGGCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCTAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAAt                                                                      <  2:317253/300‑1 (MQ=255)                                                                                                                                                                                                                              acaAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAAtt                                                                     <  1:428398/300‑1 (MQ=255)                                                                                                                                                                                                                               caAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTa                                                                    >  1:275362/1‑300 (MQ=255)                                                                                                                                                                                                                                  aagaagaagCTGGTGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCg                                                                 <  1:323437/300‑1 (MQ=255)                                                                                                                                                                                                                                  aagaagaagCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCg                                                                 <  1:864071/300‑1 (MQ=255)                                                                                                                                                                                                                                    gaagaagCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTCTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTg                                                               <  2:785103/300‑1 (MQ=255)                                                                                                                                                                                                                                        aagCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCg                                                           >  1:126778/1‑300 (MQ=255)                                                                                                                                                                                                                                            tGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTAt                                                       <  2:38599/300‑1 (MQ=255)                                                                                                                                                                                                                                             ggggCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGANTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTAtt                                                      >  1:614733/1‑300 (MQ=255)                                                                                                                                                                                                                                                 cATTTGTTTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGANCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGANTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGt                                                  <  2:806518/300‑1 (MQ=255)                                                                                                                                                                                                                                                 cATTTGTGTTGATTTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGANCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGt                                                  <  2:649428/300‑1 (MQ=255)                                                                                                                                                                                                                                                 cATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGt                                                  <  1:165563/300‑1 (MQ=255)                                                                                                                                                                                                                                                 cATTTGTGTTGATGTCCTTGGATTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTTCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGt                                                  <  2:315170/300‑1 (MQ=255)                                                                                                                                                                                                                                                 cATTTGTGGTGATGTCGTTGGAGTCCGGGCCGCTCAGGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGt                                                  <  1:113935/300‑1 (MQ=255)                                                                                                                                                                                                                                                      gtgtTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGTTGAtt                                             >  1:645012/1‑300 (MQ=255)                                                                                                                                                                                                                                                        gtTGATGTCGTGGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGTGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGTTGATTAt                                           <  2:338691/300‑1 (MQ=255)                                                                                                                                                                                                                                                           gATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTGTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGGGGATTATCGc                                        <  1:721068/300‑1 (MQ=255)                                                                                                                                                                                                                                                               tCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGTTGATTATCGCCGAt                                    >  1:770042/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           gggCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGTTGATTATCGCCGATAAATTGCTTGGc                        >  2:471842/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             gCCGCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGTTGATTATCGCCGATAAATTGATTGGCgg                      <  1:512540/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                gCTCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGTTGATTATCGCCGATAAATTGATTGGCGGTgg                   >  1:103580/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  tCATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGTTGATTATCGCCGATAAATTGATTGGCGGTGGCg                 <  1:784218/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gcATGACGATGATTATTCTGAGCACTGCCGGGATTGCCTCGGTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTCCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGTTGATTATCGCCGATAAATTGATTGGCGGTGGCg                 <  2:126246/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                       aCGATGATTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGTTGATTATCGCCGATAAATTGATTGGCGGTGGCGATGGc            <  1:246651/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         gatgatTATTCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGTTGATTATCGCCGATAAATTGATTGGCGGTGGCGATGGCAc          <  1:52555/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                attCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTNTTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGNATTCCGTTGATTATCGCCGATAAATTGATTGGCGGTGGCGATGGCACTGCCGGa   >  2:792598/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                attCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGTTGATTATCGCCGATAAATTGATTGGCGGTGGCGATGGCACTGCCGGa   >  1:551432/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 ttCTGAGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGTTGATTATCGCCGATAAATTGATTGGCGGTGGCGATGGCACTGCCGGaa  <  1:337253/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                       CAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGGCGGCGATGGATATGACCAATGGCGGACTTTACGCTTCCATCATGCAGCAGTACGGCACAAAAGAAGAAGCTGGGGCATTTGTGTTGATGTCGTTGGAGTCCGGGCCGCTCATGACGATGATTATTCTGGGCACTGCCGGGATTGCCTCGTTTGAACCGCATGTTTTCGTCGGCGCAGTATTACCGTTCCTGGTGGGCTTTGCCCTGGGGAACCTTGACCCTGAATTGCGAGAATTTTTCAGCAAAGCGGTGCAAACGCTGATTCCATTCTTTGCCTTCGCGCTGGGCAATACCATTGATTTGACTGTGATTGCCCAGACTGGTTTGCTGGGGATCCTGTTGGGTGTGGCAGTAATTATCGTGACCGGTATTCCGTTGATTATCGCCGATAAATTGATTGGCGGTGGCGATGGCACTGCCGGAA  >  NC_000913/4101871‑4102458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑