Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,255,799	G→A	S223F (TCC→TTC)	plsB ←	glycerol‑3‑phosphate 1‑O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,255,799	0	G	A	98.9%	321.7 / NA	91	S223F (TCC→TTC)	plsB	glycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (48/42);  minor base C (1/0);  total (49/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACAC  >  NC_000913/4255503‑4256094                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        ggTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAgg                                                                                                                                                                                                                                                                                                      <  1:579001/300‑1 (MQ=255)  gTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGc                                                                                                                                                                                                                                                                                                     >  1:200114/1‑300 (MQ=255)    gATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGa                                                                                                                                                                                                                                                                                                   >  1:203774/1‑300 (MQ=255)               tAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTACATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCttatca                                                                                                                                                                                                                                                                                        >  2:318601/1‑298 (MQ=255)                       cagcagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATc                                                                                                                                                                                                                                                                                <  1:385656/300‑1 (MQ=255)                          cagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTg                                                                                                                                                                                                                                                                             >  2:494651/1‑300 (MQ=255)                          cagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTg                                                                                                                                                                                                                                                                             >  1:859410/1‑300 (MQ=255)                             gTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACg                                                                                                                                                                                                                                                                          <  1:489509/300‑1 (MQ=255)                              tAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGa                                                                                                                                                                                                                                                                         >  1:329332/1‑300 (MQ=255)                                gTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACCGATCCTGACGAGc                                                                                                                                                                                                                                                                       >  1:133319/1‑300 (MQ=255)                                gTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTAGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGCCGAGc                                                                                                                                                                                                                                                                       >  2:443716/1‑300 (MQ=255)                                  ccATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAg                                                                                                                                                                                                                                                                     >  1:744202/1‑300 (MQ=255)                                       tgACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCACAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCaaa                                                                                                                                                                                                                                                                >  2:149613/1‑300 (MQ=255)                                         aCTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCANAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGCAATTCGCCGCAATCTCTTCCATCAGTNCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACg                                                                                                                                                                                                                                                              <  2:701189/300‑1 (MQ=255)                                          ccgCGGTGGCAAGGCACATATACCGGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGt                                                                                                                                                                                                                                                             <  2:184709/298‑1 (MQ=255)                                          cTGCAGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAATAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGt                                                                                                                                                                                                                                                             <  2:226239/300‑1 (MQ=255)                                              ggTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGc                                                                                                                                                                                                                                                         <  1:779477/300‑1 (MQ=255)                                               gTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGcc                                                                                                                                                                                                                                                        <  1:119589/300‑1 (MQ=255)                                               gTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGcc                                                                                                                                                                                                                                                        <  1:390258/300‑1 (MQ=255)                                                     aGGCACATATACCAGCTCATGACCGTCGGGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAATAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGc                                                                                                                                                                                                                                                  <  2:522007/300‑1 (MQ=255)                                                      ggCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCa                                                                                                                                                                                                                                                 >  2:736255/1‑300 (MQ=255)                                                      ggCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGAGCCTACGGCa                                                                                                                                                                                                                                                 >  1:331585/1‑300 (MQ=255)                                                       gCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAg                                                                                                                                                                                                                                                >  1:765459/1‑300 (MQ=255)                                                        cacaTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGc                                                                                                                                                                                                                                               >  1:349566/1‑300 (MQ=255)                                                         acaTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGcc                                                                                                                                                                                                                                              >  1:479611/1‑300 (MQ=255)                                                         acaTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGcc                                                                                                                                                                                                                                              >  2:301565/1‑300 (MQ=255)                                                         acaTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATGGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTGTACCGCTTTGGCAATGGCGCGGAAGGCNAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGcc                                                                                                                                                                                                                                              >  2:637887/1‑300 (MQ=255)                                                          caTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTACATCAGTGCAATCGCGTTCTGTTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAATGTGGGCCTACGGCCGCCa                                                                                                                                                                                                                                             >  2:301386/1‑300 (MQ=255)                                                            tataCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGa                                                                                                                                                                                                                                           >  2:781260/1‑300 (MQ=255)                                                            tataCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGa                                                                                                                                                                                                                                           >  1:338235/1‑300 (MQ=255)                                                             ataCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGAc                                                                                                                                                                                                                                          >  1:498218/1‑300 (MQ=255)                                                              tgccAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCTTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACg                                                                                                                                                                                                                                         <  2:96374/298‑1 (MQ=255)                                                              taCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACg                                                                                                                                                                                                                                         <  1:230349/300‑1 (MQ=255)                                                                   gCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGAc                                                                                                                                                                                                                                    >  1:487873/1‑300 (MQ=255)                                                                   gCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGAc                                                                                                                                                                                                                                    >  2:321772/1‑300 (MQ=255)                                                                   gCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTAATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAANTTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTNTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTACACCGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGAc                                                                                                                                                                                                                                    >  2:694123/1‑300 (MQ=255)                                                                    gtCATGAACGTGGTGGGCAAGCTGGCGAACGCGCTCAGCGTTAGGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAGTACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCAATCAGTGCAATCGAGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAAAAGATCCTGATGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACg                                                                                                                                                                                                                                   >  2:397884/2‑300 (MQ=255)                                                                    ctcccaaCCGTCGTGGGCCAGCTGACGAACGCGCTCAGCGTCATGGACGTTGATGCCCTGGTACAGTCGGTTCCAGGTGACGCACAGACTACGGCCCGTCAGACGCATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGTTGCGCTTTTTCGTGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGCAGGCGAGCAGCTTACTCAACAGCCCCTGACGAGCAGGCAAACGTGGGCCTCCAGCAACCAAACGCTGACg                                                                                                                                                                                                                                   >  2:316639/7‑300 (MQ=255)                                                                         gACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCaa                                                                                                                                                                                                                              <  1:355919/300‑1 (MQ=255)                                                                              tcgtGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTACATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGAAGAGCAGGCAAACGTGGGCATACGGCAGCCAGACGTTGACGGGCAAAGTGc                                                                                                                                                                                                                         >  2:315865/1‑300 (MQ=255)                                                                                        gCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCa                                                                                                                                                                                                               >  1:486907/1‑300 (MQ=255)                                                                                                    gcTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGACAAGCGCGCCAGtt                                                                                                                                                                                                   >  1:89470/1‑300 (MQ=255)                                                                                                    gcTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAAGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGTGCCAgct                                                                                                                                                                                                   >  2:326953/1‑298 (MQ=255)                                                                                                         gCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGa                                                                                                                                                                                              >  1:190544/1‑300 (MQ=255)                                                                                                         gCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGa                                                                                                                                                                                              >  1:412884/1‑300 (MQ=255)                                                                                                          cGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGNGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGACAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAg                                                                                                                                                                                             <  1:622213/300‑1 (MQ=255)                                                                                                                    gTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGtttt                                                                                                                                                                                   >  1:483329/1‑300 (MQ=255)                                                                                                                        aTGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATcc                                                                                                                                                                               <  2:459102/300‑1 (MQ=255)                                                                                                                          gCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATccgt                                                                                                                                                                             >  1:3312/1‑300 (MQ=255)                                                                                                                          gCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTACATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCCTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATccgt                                                                                                                                                                             >  2:151722/1‑300 (MQ=255)                                                                                                                            ccTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATccgtgc                                                                                                                                                                           <  1:855696/300‑1 (MQ=255)                                                                                                                                      tCGGTTCCAGGTGAAGCCCAGACTACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATTGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATc                                                                                                                                                                 <  2:178528/300‑1 (MQ=255)                                                                                                                                      tCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATc                                                                                                                                                                 <  1:156922/300‑1 (MQ=255)                                                                                                                                         gTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGc                                                                                                                                                              >  2:499515/1‑300 (MQ=255)                                                                                                                                         gTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTACATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGc                                                                                                                                                              >  2:244868/1‑300 (MQ=255)                                                                                                                                              aGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATAc                                                                                                                                                         >  2:488175/1‑300 (MQ=255)                                                                                                                                                 tGAAGCCCAGAATACGTTCAGTCAGGCGAATCATCTCTTAAGAGAAATTCGCCGCAATCTCCTCCATCAGTGCAATCGCGTTCTGCTTCGCTTTTTCATTGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGTGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGgc                                                                                                                                                      <  2:86936/300‑1 (MQ=255)                                                                                                                                                 tGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAATAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGANTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGgc                                                                                                                                                      <  2:767133/300‑1 (MQ=255)                                                                                                                                                         aGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGaa                                                                                                                                              >  1:47254/1‑300 (MQ=255)                                                                                                                                                         aGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGaa                                                                                                                                              >  2:554132/1‑300 (MQ=255)                                                                                                                                                          gAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGaaa                                                                                                                                             >  2:372719/1‑300 (MQ=255)                                                                                                                                                          gAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGaaa                                                                                                                                             >  1:132885/1‑300 (MQ=255)                                                                                                                                                             tACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCGTTTTCATGGGAGATTTTTTTGCTGCGCGCNTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTAGACGTGCCGGGTTcgtatgcaaggcggggaggggagagtg                                                                                                                                          <  1:603518/300‑27 (MQ=255)                                                                                                                                                                   cagtcagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCg                                                                                                                                    <  1:300104/300‑1 (MQ=255)                                                                                                                                                                         ggCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACNGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGgcctgtctcttatacacatctcgcag                                                                                                                              <  1:638558/300‑25 (MQ=255)                                                                                                                                                                             aaTCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCACGCTTCATCTTCTACCGCTTTGACAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCCGACGAGCAGGCAAACGCGGGCCTACGGCAGCCGGCCGTTGACGGGCAAAGTGCCTACGCGCCGCGCGCGCCAGTGTCTGAGCGATAGTTTTCTCCGTGTCGTGTTCGTCCGCAATACGGCGCAGCGAAACTGACGGCgagagagccac                                                                                                                          >  2:249828/1‑293 (MQ=255)                                                                                                                                                                                  tctcGTAATAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATTGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAc                                                                                                                     <  1:430777/300‑1 (MQ=255)                                                                                                                                                                                  tctcGTAATAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAc                                                                                                                     <  2:281517/300‑1 (MQ=255)                                                                                                                                                                                  tctcGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAc                                                                                                                     <  1:430786/300‑1 (MQ=255)                                                                                                                                                                                                     cgcAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAg                                                                                                  <  1:542897/300‑1 (MQ=255)                                                                                                                                                                                                      gcAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGTGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGt                                                                                                 <  2:99196/300‑1 (MQ=255)                                                                                                                                                                                                      gcAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGt                                                                                                 <  2:253514/300‑1 (MQ=255)                                                                                                                                                                                                      gcAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGt                                                                                                 <  1:652928/300‑1 (MQ=255)                                                                                                                                                                                                      gcAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGt                                                                                                 <  1:683961/300‑1 (MQ=255)                                                                                                                                                                                                        aaTCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTAc                                                                                               <  1:439754/300‑1 (MQ=255)                                                                                                                                                                                                        aaTCTCTTCCATCAGTGCAATCGCGGTCTGCTGCGCTTGTTCATTGTAGATTGTTTTGCTGCGCGCTTCATCTTCTACCGCTTGGGCAATGGCGCGGAAGGCGAGCAGCTTAGTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTNCGGCAGCCAGACGTTGACGGGCAAAGNGCATACGCGCCACGCGCGCCAGTTTCGGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTAc                                                                                               <  2:594962/300‑1 (MQ=255)                                                                                                                                                                                                           ctctTCCATCATTGAAATGGCGTTCTGTTGCGCTTTGTCATTGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGGTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGc                                                                                            <  1:651507/300‑1 (MQ=255)                                                                                                                                                                                                           ctctTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCANCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACANTACAGc                                                                                            >  2:709526/1‑300 (MQ=255)                                                                                                                                                                                                           ctctTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGc                                                                                            >  1:341744/1‑300 (MQ=255)                                                                                                                                                                                                           ctctTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTAGCGACCGAGCCACAGTACAGc                                                                                            >  2:485709/1‑300 (MQ=255)                                                                                                                                                                                                                                   ctgctgCGCCTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAACACTGTCGCAACCGAGCCACAGCACAACGAAAAACTTCTGTACGCCGTTAAg                                                                    >  2:330989/1‑300 (MQ=255)                                                                                                                                                                                                                                    ttctgcGCTTTTTCATTGGAGATTTTTTTGCTGCGCGCTTCATCTGCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTaacc                                                                   <  2:733197/298‑3 (MQ=255)                                                                                                                                                                                                                                    tgctgcNCTTTTTCATTGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTNTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGc                                                                   <  2:717509/300‑1 (MQ=255)                                                                                                                                                                                                                                    tgctgcGCTTTTTCGTTGGAGATTGTTTTGCTGCGCGCTTCACCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGGGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCGGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGc                                                                   <  2:64294/300‑1 (MQ=255)                                                                                                                                                                                                                                    tgctgcGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGc                                                                   <  1:503440/300‑1 (MQ=255)                                                                                                                                                                                                                                      ctgcGCTTTTTCATTGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCAt                                                                 <  2:372222/300‑1 (MQ=255)                                                                                                                                                                                                                                                         aTTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACt                                              <  1:523279/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 tctACCGCTTTGGCAATGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATNCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGgcgcg                      >  2:219079/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               aaTGGCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGNGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCAc        <  2:748371/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  ggCGCGGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGa     >  1:130420/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     gcgGAAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGacac  <  1:701757/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        GGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACAC  >  NC_000913/4255503‑4256094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑