Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,422,619	G→A	G145D (GGC→GAC)	ulaD →	3‑keto‑L‑gulonate‑6‑phosphate decarboxylase UlaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,422,619	0	G	A	100.0%	201.0 / NA	56	G145D (GGC→GAC)	ulaD	3‑keto‑L‑gulonate‑6‑phosphate decarboxylase UlaD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/33);  total (23/33)

CGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCAACGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGGCGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCT  >  NC_000913/4422327‑4422910                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                    cGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCAACGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAgacgtggcg                                                                                                                                                                                                                                                                                              <  1:481949/300‑1 (MQ=255)    gACCTGAAAGCGCTCTACCCGCACAAAATTGTACTGGCAGACGCCAAAGTTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCAACGCCGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACTGGTTACTGGACCTGGGAACAGGCGCAACATTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACACGCGGGCCGCAGAcgtggcgtgg                                                                                                                                                                                                                                                                                           <  2:351225/300‑1 (MQ=255)                        gCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCAACGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACcgc                                                                                                                                                                                                                                                                       >  1:460262/1‑300 (MQ=255)                                 cGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCAACGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACg                                                                                                                                                                                                                                                              <  1:238049/300‑1 (MQ=255)                                  gTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCAACGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGTGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGt                                                                                                                                                                                                                                                             >  1:133967/1‑300 (MQ=255)                                                      tGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCAACGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCaa                                                                                                                                                                                                                                         <  1:414182/300‑1 (MQ=255)                                                        ccGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCAACGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAg                                                                                                                                                                                                                                       <  1:855538/300‑1 (MQ=255)                                                                  cAAAATCCTTTCGCGTATGTGCTTCGAAACCAACGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACTGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATTACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAgtcaccgtcac                                                                                                                                                                                                                             <  2:72286/300‑1 (MQ=255)                                                                                             aGCCAACGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCTCACCCTTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGcc                                                                                                                                                                                                  >  2:305071/1‑300 (MQ=255)                                                                                             aGCCAACGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGcc                                                                                                                                                                                                  >  2:559430/1‑300 (MQ=255)                                                                                                       gACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAg                                                                                                                                                                                        >  2:442106/1‑300 (MQ=255)                                                                                                         cTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAggg                                                                                                                                                                                      <  1:35284/300‑1 (MQ=255)                                                                                                         cTGGGGGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGNACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACNCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAggg                                                                                                                                                                                      <  2:688648/300‑1 (MQ=255)                                                                                                              tGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGGTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGAGGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCTTGACGCGCAGGCCGCAGACTTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTTACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTAtgc                                                                                                                                                                                 <  1:425036/300‑3 (MQ=255)                                                                                                               gACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTcc                                                                                                                                                                                <  1:244490/300‑1 (MQ=255)                                                                                                                       tttGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACTGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACg                                                                                                                                                                        <  2:34785/300‑1 (MQ=255)                                                                                                                                cGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATcg                                                                                                                                                               <  1:694002/300‑1 (MQ=255)                                                                                                                                    tatCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCggg                                                                                                                                                           >  2:299422/1‑300 (MQ=255)                                                                                                                                    tatCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCAACGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCggg                                                                                                                                                           >  2:485215/1‑300 (MQ=255)                                                                                                                                            ccGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGTGCCgtagta                                                                                                                                                   <  2:520554/300‑1 (MQ=255)                                                                                                                                                cAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCg                                                                                                                                               <  1:73778/300‑1 (MQ=255)                                                                                                                                                  aaGGCGCGTTGTACGTGGCAAAAGAGTTTAACGGCGACGGGCAGATCGAACCGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCGAGGGTATTCCGATTCACGTCTTGATCGCGGGCCGTAGTATCCGTg                                                                                                                                             <  2:256444/300‑1 (MQ=255)                                                                                                                                                  aaGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGGCCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTg                                                                                                                                             <  1:570289/300‑1 (MQ=255)                                                                                                                                                  aaGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACNGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATNCACGTCTTTATCGCGGGCCGTAGTATCCGTg                                                                                                                                             <  2:624669/300‑1 (MQ=255)                                                                                                                                                  aaGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTg                                                                                                                                             <  1:570597/300‑1 (MQ=255)                                                                                                                                                                 tGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCgg                                                                                                                              >  1:514081/1‑300 (MQ=255)                                                                                                                                                                   gCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTg                                                                                                                            <  1:316454/300‑1 (MQ=255)                                                                                                                                                                    cAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTgg                                                                                                                           <  2:363855/300‑1 (MQ=255)                                                                                                                                                                                        gTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGAGTGGGGCGAAGCGGACATCACCGCGATCAAACGCCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTCTCGCNGGCCGTCGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGGTCaaa                                                                                                       >  2:631181/1‑300 (MQ=255)                                                                                                                                                                                             gATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGNTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTc                                                                                                  >  1:580240/1‑300 (MQ=255)                                                                                                                                                                                                      gACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGa                                                                                         >  1:229787/1‑300 (MQ=255)                                                                                                                                                                                                           gTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACtgt                                                                                    >  1:706297/1‑300 (MQ=255)                                                                                                                                                                                                           gTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCAGGTGGAAGCAGCACGTCAGTTCAGACGTTAAATCGCTGaaatat                                                                                    >  2:260799/1‑296 (MQ=255)                                                                                                                                                                                                                tGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGc                                                                               <  1:111654/300‑1 (MQ=255)                                                                                                                                                                                                                  gACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTa                                                                             >  1:736779/1‑300 (MQ=255)                                                                                                                                                                                                                    ccTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCAACGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGCAGCCGCACGTTAGTTCAAACGTTCCATAGATGAACTGGGGGGCGAAg                                                                           >  2:444558/1‑300 (MQ=255)                                                                                                                                                                                                                     cTGGGAACAGGCGCAACAGTGGCTCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGATATCACCGCGATCAAACGTCTTTCCGATATGGGATTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATGCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                          <  2:433358/300‑1 (MQ=255)                                                                                                                                                                                                                     cTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                          <  1:566270/300‑1 (MQ=255)                                                                                                                                                                                                                     cTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCAGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                          <  1:311979/300‑1 (MQ=255)                                                                                                                                                                                                                       gggAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGTAGGTGGGTTATCATCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCGCCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGGACTGTGGGGCTAAGGAg                                                                        <  2:833331/300‑1 (MQ=255)                                                                                                                                                                                                                             aGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCNATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGata                                                                  >  1:580367/1‑300 (MQ=255)                                                                                                                                                                                                                               gcgcAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATAtg                                                                <  1:313561/300‑1 (MQ=255)                                                                                                                                                                                                                                cgcAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATAtgt                                                               <  1:780238/300‑1 (MQ=255)                                                                                                                                                                                                                                     cAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTcc                                                          <  1:409909/300‑1 (MQ=255)                                                                                                                                                                                                                                          gcgcGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCcaaaca                                                     <  1:417667/300‑1 (MQ=255)                                                                                                                                                                                                                                               aggcaggtagtGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGAGATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCGGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTAATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATcc                                                <  2:57509/290‑1 (MQ=255)                                                                                                                                                                                                                                               aggcaggcagGGGGAAGGTGGTTTATAACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGTACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTGCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATcc                                                <  2:836204/298‑1 (MQ=255)                                                                                                                                                                                                                                               aTGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCATGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATcc                                                <  1:803500/300‑1 (MQ=255)                                                                                                                                                                                                                                               aTGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATcc                                                >  1:171386/1‑300 (MQ=255)                                                                                                                                                                                                                                                    ggCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCtt                                           >  2:851609/1‑300 (MQ=255)                                                                                                                                                                                                                                                               ggtggtTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGa                                >  1:329085/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 tggtTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGCAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGaaa                              >  2:827677/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  ggtTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTTGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGaaaa                             <  1:22055/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  ggtTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCAGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGaaaa                             >  2:3323/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   gtTTATCACCGCAGCCGTGACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGaaaaa                            >  1:358432/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     gACGCGCAGGCCGCAGACGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAACGCGCTCCCCGCCGGGGAg          >  1:762802/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        gcgcAGGCCGCAGACGTGGCGTTGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCTGAGGCCTGGCGCTGGAAGATCNGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgc       <  1:600245/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             ggccgcagacGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggct  <  1:214062/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             ggccgcagacGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCAGGTGGAAGCAGCACGTCAGTTCAAACGTTCCATCGATGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCTCGATTGGCATCTATGAAAAAGCGCTCCCCGACGGGGAGTgctggct  >  2:662554/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             ggccgcagacGGGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggct  <  1:448630/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                    CGTGACCTGAAAGCGCTCTACCCGCACAAAATCGTACTGGCAGACGCCAAAATTGCCGATGCAGGCAAAATCCTTTCGCGTATGTGCTTCGAAGCCAACGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGGCGTGGCGTGGGGCGAAGCGGACATCACCGCGATCAAACGTCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCT  >  NC_000913/4422327‑4422910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑