Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,296,481	G→A	V25M (GTG→ATG)	dolP →	division and outer membrane stress‑associated lipid‑binding lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,296,481	0	G	A	100.0%	297.5 / NA	84	V25M (GTG→ATG)	dolP	division and outer membrane stress‑associated lipid‑binding lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/40);  total (44/40)

AAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACGAAGTGTATAACGAGATTCGTCAGGGCCAGCCGATTGGTCTGGGCGAAGCA  >  NC_000913/3296197‑3296774                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                      aaGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGTCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGctgctg                                                                                                                                                                                                                                                                                        <  2:150065/300‑1 (MQ=255) aaGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGctgctg                                                                                                                                                                                                                                                                                        <  1:440969/300‑1 (MQ=255)      gccgTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGt                                                                                                                                                                                                                                                                                   <  1:216830/300‑1 (MQ=255)      gccgTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATAGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGt                                                                                                                                                                                                                                                                                   <  1:665318/300‑1 (MQ=255)         gTTACGCTTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTAcc                                                                                                                                                                                                                                                                                <  1:180105/300‑1 (MQ=255)         gTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTAcc                                                                                                                                                                                                                                                                                <  1:658538/300‑1 (MQ=255)           tACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGTAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCaa                                                                                                                                                                                                                                                                              <  1:83506/300‑1 (MQ=255)           tACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCaa                                                                                                                                                                                                                                                                              <  1:516460/300‑1 (MQ=255)                 tGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGc                                                                                                                                                                                                                                                                        >  2:776142/1‑300 (MQ=255)                  gctatGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCa                                                                                                                                                                                                                                                                       >  1:651294/3‑300 (MQ=255)                         ccATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGAcc                                                                                                                                                                                                                                                                >  1:539524/1‑300 (MQ=255)                         ccATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCATGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGAcc                                                                                                                                                                                                                                                                <  1:492534/300‑1 (MQ=255)                            ttGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCAc                                                                                                                                                                                                                                                             >  2:157327/1‑300 (MQ=255)                            ttGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCAc                                                                                                                                                                                                                                                             >  1:405742/1‑300 (MQ=255)                            ttGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCAACAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCAc                                                                                                                                                                                                                                                             >  2:367539/1‑300 (MQ=255)                            ttGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAANTGCCTGTGCGATCTGATCGATAACACNCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCAc                                                                                                                                                                                                                                                             >  2:595526/1‑300 (MQ=255)                                   aTTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAgtgt                                                                                                                                                                                                                                                      >  1:466437/1‑300 (MQ=255)                                   aTTGACCGGCCACGACAGCGGCGAACTTGCAGGTTTTTTAGGGCCACAAGATGTGTAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAgtgt                                                                                                                                                                                                                                                      <  2:265552/300‑1 (MQ=255)                                         cGGCCATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGANAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCAc                                                                                                                                                                                                                                                <  2:218795/300‑1 (MQ=255)                                                gACGGCGGCGAACTGGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTg                                                                                                                                                                                                                                         <  1:825904/300‑1 (MQ=255)                                                            cTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTAcc                                                                                                                                                                                                                             >  2:511806/1‑300 (MQ=255)                                                             ttGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTANTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGNTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTAccc                                                                                                                                                                                                                            <  2:790508/300‑1 (MQ=255)                                                             ttGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGTAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTAccc                                                                                                                                                                                                                            <  2:340466/300‑1 (MQ=255)                                                                cAGGTTTGTTATGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCCCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTgg                                                                                                                                                                                                                         <  2:30260/300‑1 (MQ=255)                                                                 aGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGa                                                                                                                                                                                                                        <  1:657913/300‑1 (MQ=255)                                                                   gtgtGTTAGTGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATNAAGGCATTATCGCCAATCGCAGTCCTNATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAg                                                                                                                                                                                                                      <  2:570297/297‑1 (MQ=255)                                                                       gTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTgcg                                                                                                                                                                                                                  <  1:333193/300‑1 (MQ=255)                                                                           gggCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGATGGTAGATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTg                                                                                                                                                                                                              <  2:416561/300‑1 (MQ=255)                                                                                 caGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCAGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAgc                                                                                                                                                                                                        >  1:137613/1‑300 (MQ=255)                                                                                   ggATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTNCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAgcgc                                                                                                                                                                                                      >  1:614988/1‑300 (MQ=255)                                                                                     aTGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCAt                                                                                                                                                                                                    >  1:834995/1‑300 (MQ=255)                                                                                     aTGTGGAGATCCGCATTCCTTCGCATCGTAGNGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCAACAGGATGATTAAGGAGANTACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCAt                                                                                                                                                                                                    >  2:615508/1‑300 (MQ=255)                                                                                            gaTCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGaa                                                                                                                                                                                             >  2:533597/1‑300 (MQ=255)                                                                                               ccGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGTTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGAGGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGa                                                                                                                                                                                          <  2:395811/300‑1 (MQ=255)                                                                                                 gCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACg                                                                                                                                                                                        >  1:444981/1‑300 (MQ=255)                                                                                                           gCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTaa                                                                                                                                                                              >  2:403409/1‑300 (MQ=255)                                                                                                              tCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTANTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACNGCGCATTGTCGAAAGACGAACAGATTaagaa                                                                                                                                                                           <  2:615803/300‑1 (MQ=255)                                                                                                              tCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTaagaa                                                                                                                                                                           <  1:409102/300‑1 (MQ=255)                                                                                                                      cTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAgcgc                                                                                                                                                                   >  1:309912/1‑300 (MQ=255)                                                                                                                          cATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCAt                                                                                                                                                               >  1:182255/1‑300 (MQ=255)                                                                                                                              cAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAAt                                                                                                                                                           >  1:739885/1‑300 (MQ=255)                                                                                                                                ggAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCANTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGt                                                                                                                                                         <  1:607796/300‑1 (MQ=255)                                                                                                                                 gAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTa                                                                                                                                                        >  1:528100/1‑300 (MQ=255)                                                                                                                                    aTGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGAAGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACg                                                                                                                                                     >  1:395178/1‑300 (MQ=255)                                                                                                                                     tGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACgg                                                                                                                                                    >  1:25626/1‑300 (MQ=255)                                                                                                                                      gCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGc                                                                                                                                                   >  1:408786/1‑300 (MQ=255)                                                                                                                                             cggACGGCAAATTGCCTGGGCGACCTGATCGATAACATGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTTTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAg                                                                                                                                            <  2:382096/298‑1 (MQ=255)                                                                                                                                             cTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAATGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAg                                                                                                                                            <  2:301975/300‑1 (MQ=255)                                                                                                                                             cTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAg                                                                                                                                            <  2:229187/300‑1 (MQ=255)                                                                                                                                                            cTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGctgctg                                                                                                                             <  1:865234/300‑1 (MQ=255)                                                                                                                                                              gtgCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGt                                                                                                                           <  1:740708/300‑1 (MQ=255)                                                                                                                                                                       gcgagatgtgtataagagacagcACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTc                                                                                                                  >  1:504399/23‑300 (MQ=255)                                                                                                                                                                            aTAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGAAGAACAGATTAAGAAAGAAGCGNGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGNTACCaa                                                                                                             >  2:720409/1‑300 (MQ=255)                                                                                                                                                                                        tCCCTCACCAGGATGATTAAGGAGAATACCTGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGAAGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACttt                                                                                                 >  1:443790/1‑300 (MQ=255)                                                                                                                                                                                            tCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTCAGGAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGc                                                                                             >  2:393804/1‑300 (MQ=255)                                                                                                                                                                                             cACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAGCGGTCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCt                                                                                            >  2:70153/1‑300 (MQ=255)                                                                                                                                                                                             cACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCt                                                                                            >  1:510175/1‑300 (MQ=255)                                                                                                                                                                                                  ggATGATCCAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGATTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCCGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTAGGCACCCAGGTGGACGATGGTACCCTGAAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGCACCGATTAAGAAAGAAACGCGCATTAATGTACCGGTATATCAGGGCAAAGTGCTGCTGATTGGGCAGTCACAAAATGCTGAACTTTTGGCTTgcgc                                                                                       >  2:30015/1‑300 (MQ=255)                                                                                                                                                                                                     tgatTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCaa                                                                                    >  1:141303/1‑300 (MQ=255)                                                                                                                                                                                                      gatTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCaaa                                                                                   >  1:808303/1‑300 (MQ=255)                                                                                                                                                                                                      gatTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTAGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAAAGGCATATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCaaa                                                                                   >  2:447087/1‑300 (MQ=255)                                                                                                                                                                                                             gcgaATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTTCTTCTGGTTGGGCAGTCCCCCAATGCTGAACTTTCGGCTCGCGCCAAACAGATTg                                                                            >  2:233069/3‑300 (MQ=255)                                                                                                                                                                                                                    cATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATggg                                                                     >  2:381252/1‑300 (MQ=255)                                                                                                                                                                                                                          ggCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCTTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCATGGCAAAATGCTGCTGGTTGGGCAGTCACCAAATACTGAACTTTCGGCTCGCGCCAAACGGATTGCGATGGGCGTAgg                                                               <  2:305494/300‑2 (MQ=255)                                                                                                                                                                                                                           gCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACNCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGAc                                                              <  1:632802/300‑1 (MQ=255)                                                                                                                                                                                                                           gCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAATAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGAc                                                              <  2:180602/300‑1 (MQ=255)                                                                                                                                                                                                                           gCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGAc                                                              <  1:245353/300‑1 (MQ=255)                                                                                                                                                                                                                           gCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGNGCGCGTGAACAGCGCATTGTCGAAAGNCGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGAc                                                              <  2:589972/300‑1 (MQ=255)                                                                                                                                                                                                                           acATTATCGCCAAGCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACCGACCCACGCAGTGTCTGCACCCAGGTGGACGATGGTACCCTGGAAGGGCGCGTGAACAGCGCATGGGCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCATGGCAAAGTGCGGCTGGTTGGGCAGTCACCAAATGCTGAACTTGCGGCTCGCGCCAAACAGATTGCTAGGGGCGGAGAc                                                              <  1:555723/299‑1 (MQ=255)                                                                                                                                                                                                                                     cAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACg                                                    <  1:334848/300‑1 (MQ=255)                                                                                                                                                                                                                                           cAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGCAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACGAAgtgt                                              >  2:73284/1‑300 (MQ=255)                                                                                                                                                                                                                                           cAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTAAACAACGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACGAAgtgt                                              >  2:74290/1‑300 (MQ=255)                                                                                                                                                                                                                                             gTCCTTATTTCCGCGCTGCTGTTGCAAGTTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACGAAGTGtat                                            <  1:748593/300‑1 (MQ=255)                                                                                                                                                                                                                                                  tATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACGAAGTGTATAACga                                       <  1:778378/300‑1 (MQ=255)                                                                                                                                                                                                                                                   aTTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGTTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACGAAGTGTATAACgag                                      <  2:438123/300‑1 (MQ=255)                                                                                                                                                                                                                                                            ctgctgTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAANCAGATTGCTATGGGCGTAGACGGTGCCAACGAAGTGTATAACGAGATNCGtaag                             >  2:711333/1‑297 (MQ=255)                                                                                                                                                                                                                                                              gctgTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACGAAGTGTATAACGAGATTCGTCAggg                           >  2:504179/1‑300 (MQ=255)                                                                                                                                                                                                                                                              gctgTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACGAAGTGTATAACGAGATTCGTAAggg                           >  2:672577/1‑300 (MQ=255)                                                                                                                                                                                                                                                              gctgTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAAAGAAGTGTATAACGAGATTCGTAAggg                           >  2:50973/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 gTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACGAAGTGTATAACGAGATTCGTCAGGGCCa                        >  1:167958/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 gTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATACTGAACTTTCGGCTCGCGCCAAACAGANTGATATGGGCGTAGACGGTGCCAACGAAGTGTATAACGAGATTCGTCAGGGCCa                        >  2:634379/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            gtgtTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACNGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACGAAGTGTATAACGAGATTCGTCAGGGCCAGCCGATTGGTc             <  2:214187/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACGAAGTGTATAACGAGATTCGTCAGGGCCAGCCGATTGGTCTGGGc        <  1:729568/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   cGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCANTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACGAAGTGTATAACGAGATTCGTCAGGGCCAGCCGATTGGTCTGGGCGa      <  1:614839/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       gCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAANGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACGAAGTGTATAACGAGATTCGTCAGGGCCAGCCGATTGGTCTGGGCGAAGCa  >  2:753053/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                      AAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACGAAGTGTATAACGAGATTCGTCAGGGCCAGCCGATTGGTCTGGGCGAAGCA  >  NC_000913/3296197‑3296774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑