Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,474,021	G→A	I11I (ATC→ATT)	bdcA ←	c‑di‑GMP‑binding biofilm dispersal mediator protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,474,021	0	G	A	100.0%	279.1 / NA	76	I11I (ATC→ATT)	bdcA	c‑di‑GMP‑binding biofilm dispersal mediator protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/34);  total (42/34)

TGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTG  >  NC_000913/4473731‑4474307                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                               tGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACt                                                                                                                                                                                                                                                                                       >  1:834639/1‑300 (MQ=255)                              atCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCATGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGAc                                                                                                                                                                                                                                                          <  1:367472/300‑1 (MQ=255)                              atCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGTTTACTAAACGACGTACGATATCTGCACCGATACCACGACGGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGAc                                                                                                                                                                                                                                                          <  2:825626/300‑1 (MQ=255)                              atCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCGTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCNGTCGCTCTATTCTCTTGTGCCAGGCGTTTATCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTTGCCCCATCGTTTACGAAACGACCTACAATAGCTGCACCAATAATACGACGGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGAc                                                                                                                                                                                                                                                          <  2:193893/300‑1 (MQ=255)                              atCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCGGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTTGCCCCATCGTTGACTAAACGACGTACCATATCTGTATTGATGCTACGGCGGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGAc                                                                                                                                                                                                                                                          <  2:400831/300‑1 (MQ=255)                                     gCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAATTGTATTACCTGTAAAAGCGCCCATAAAGACTCCTTGa                                                                                                                                                                                                                                                   >  1:751256/1‑300 (MQ=255)                                             ttCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTtattat                                                                                                                                                                                                                                           >  1:30112/1‑300 (MQ=255)                                                 aGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGAATATCCAATGCGCCGTTCCTACGAACGGCATCAATGACAGCGCCCCTGTCAGCACTATCTGTGAATACTGCTTTCGCTCTAGTCTCTTGTGCCAGGCATTTAGCTGCACCTTTCGACCCCACATAGGTGAATCGTACATTTGCCCCACCGGTGACGAAACGACGTACGCCCGCGGCACCAATACCACGACGACCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTaa                                                                                                                                                                                                                                       <  2:81530/300‑1 (MQ=255)                                                           cAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTAc                                                                                                                                                                                                                             <  1:302261/300‑1 (MQ=255)                                                                aCGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTNTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGTTGACGAAACGACGTACGATAGCGGCACCGATACCACGACGGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAAc                                                                                                                                                                                                                        <  2:770481/300‑1 (MQ=255)                                                                       tACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTa                                                                                                                                                                                                                 >  1:316828/1‑300 (MQ=255)                                                                          cTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACt                                                                                                                                                                                                              <  1:175609/300‑1 (MQ=255)                                                                          cTGCATTTACCACCAGGATATCCAACGCGCCGCTCTTACGAACGACAGCAATTACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTTTCGCTCGAGTCTCTTGTGCCAGGCGTTTAGCTGCATCTCTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGTTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCGTGATTTATTATGTAACATGCATTACAAAACTGTTTTagct                                                                                                                                                                                                              <  1:108949/300‑4 (MQ=255)                                                                                   ccaccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCaa                                                                                                                                                                                                     >  1:409366/1‑300 (MQ=255)                                                                                    caccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAAc                                                                                                                                                                                                    >  1:483630/1‑300 (MQ=255)                                                                                              tCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTa                                                                                                                                                                                          <  1:670396/300‑1 (MQ=255)                                                                                               ccAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTaa                                                                                                                                                                                         <  2:814626/300‑1 (MQ=255)                                                                                                         gcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTaa                                                                                                                                                                               >  1:451699/1‑300 (MQ=255)                                                                                                         gcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACCGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTaa                                                                                                                                                                               >  1:20120/1‑300 (MQ=255)                                                                                                         gcTCCTACGAACGACAGCAATGACAGCGTCTCTGTCAGCACTACCTGTGAATACTGCTTTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCTCATAGTTGAATCGTACATTGTCCCCCCCTTTGACTAAACCCCGCACCCTCTCTGCACCGATGACACGGCGGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTaa                                                                                                                                                                               <  2:333377/300‑1 (MQ=255)                                                                                                              tACGAACTACATCAGCGACATCCTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCATTCGTTTATCTGCATCTTTCGACCCCGCATAGTTGAATCGTACATCTTCCCCATCTTTGACTAAACTACGTACTATATCTGCACCAATGCCACGACGACCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAc                                                                                                                                                                          <  2:99969/300‑1 (MQ=255)                                                                                                                    cgaCATCAATGACAGCGTCTATGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAAtctc                                                                                                                                                                    >  1:656483/1‑300 (MQ=255)                                                                                                                        aTCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGt                                                                                                                                                                >  1:7462/1‑300 (MQ=255)                                                                                                                               aCAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGgt                                                                                                                                                         >  1:488034/1‑300 (MQ=255)                                                                                                                                taGCGTCTCTGTCGGCGCTATCTGTGAATCCTGTTGTCGCTTTAGTCTTTTGTGCTAGGCGTTTGGCGGCATCTTTCGACCCCGCATAGGTGAATCGTCCCTTGTCCCCATCGTTGACTAACCGACTTACGATCGCTGTGCTGATGCTATGGCGGCCACCGAGAATGAGAACTGTCTTGCCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTTCAGgtt                                                                                                                                                        <  2:91789/299‑2 (MQ=255)                                                                                                                                cagcgtctcgggcgacACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTGGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTTGCCCCATCGTTGACGAAACTACGTACGATATCTGCACCGATACCACGACGACCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGgtc                                                                                                                                                        <  2:562339/285‑1 (MQ=255)                                                                                                                                cAGCTTCTCTGTCAGCACTATCTGTGAATACTGCTTTCGCTCTAGTCTCTTGTGCCATTCGTTTAGCGGCATCTTTCGACCCCCCATATGTGAATCATACATTGGCCCCATCGTCTACGAAACCACCTACCATACCTGCACCAATACCACGACGACCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGgtc                                                                                                                                                        <  2:393293/300‑1 (MQ=255)                                                                                                                                cAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGgtc                                                                                                                                                        <  1:473206/300‑1 (MQ=255)                                                                                                                                    gTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTGGTCTCTTGTGCCAGNCGTTTAGCTGCATCTTTCGACCCCGCAGAGGTGAATCGTACATTGGCCCCATCGTTGACGAAACGACGTACGACCCCGGCACCAATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTcc                                                                                                                                                    <  2:635210/300‑1 (MQ=255)                                                                                                                                    gTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTcc                                                                                                                                                    <  1:385404/300‑1 (MQ=255)                                                                                                                                        ctGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATCAAGACTCATTGATTTATTATGTAACGTGCATTACAAAACTGTTTTAACTTTCTGTCACCAGGTTTTGTAATGGTCAATAAAAAACAATCTCGCGTTCCAGGTCGTccccgc                                                                                                                                                >  1:288219/1‑297 (MQ=255)                                                                                                                                         ttgtagtgcggtttgtgagtgctgttgTCGCTCTAGTCTCTTGTTCTAGGCGTTTATCGGCATCTTTCGGCCCCGCATAGGTGAATCGTACATTGGCCCCATCGTTGACTAAACGACTTACGCTATCGTCACTAATGCCACGGCGGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGAc                                                                                                                                               <  2:221660/275‑1 (MQ=255)                                                                                                                                         tGTCAGCACTATCTGTGAATACTGCTGTCGCTCTCGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAACAGCGCCCATAAAGAATCCTTGATTTATTATGTAGCATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAGTGGTTACTAAAAAACAATCTAGTGTTCCAGGTCGTCTCAGAc                                                                                                                                               >  2:503128/1‑300 (MQ=255)                                                                                                                                         tGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGAc                                                                                                                                               >  1:325445/1‑300 (MQ=255)                                                                                                                                          gTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTCGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTTGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACg                                                                                                                                              <  2:814992/300‑1 (MQ=255)                                                                                                                                              gCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAACGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTc                                                                                                                                          >  1:496553/1‑300 (MQ=255)                                                                                                                                              gCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTc                                                                                                                                          >  1:498227/1‑300 (MQ=255)                                                                                                                                                 cTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCt                                                                                                                                       >  1:256403/1‑300 (MQ=255)                                                                                                                                                   aTCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCCACAGGTTTTGTAGTGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTcc                                                                                                                                     >  1:257183/1‑300 (MQ=255)                                                                                                                                                        tgAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGc                                                                                                                                <  1:567799/300‑1 (MQ=255)                                                                                                                                                         gAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTAACTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCAAGACGTTTCGCTCCTGAGCa                                                                                                                               >  1:241374/1‑300 (MQ=255)                                                                                                                                                          aaTACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAg                                                                                                                              <  1:756320/300‑1 (MQ=255)                                                                                                                                                             aCTGCTTTAGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCCTTCGACCCCGCATAGGTGAATCGTACATTGTCCCCATCGGTTACGAAACGACGTACGATAGCTGCACCAATACCACGACGACCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCa                                                                                                                           <  2:105010/300‑1 (MQ=255)                                                                                                                                                              ctgctgTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGTCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCaa                                                                                                                          >  1:225424/1‑300 (MQ=255)                                                                                                                                                                gctgTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAtc                                                                                                                        >  1:49686/1‑300 (MQ=255)                                                                                                                                                                      gCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCg                                                                                                                  >  1:306255/1‑300 (MQ=255)                                                                                                                                                                       ctctaGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTNCAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCgg                                                                                                                 >  1:594168/5‑300 (MQ=255)                                                                                                                                                                           aGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaaa                                                                                                             >  1:308486/1‑300 (MQ=255)                                                                                                                                                                              ctctTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGt                                                                                                          >  1:502339/1‑300 (MQ=255)                                                                                                                                                                                     gCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt                                                                                                   >  1:149379/1‑300 (MQ=255)                                                                                                                                                                                                        cTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATg                                                                                <  2:551394/300‑1 (MQ=255)                                                                                                                                                                                                                 ccGCATAGGTGAATCGTACATTGGCCCCATCTGTGACGAAACGACGTACGATAGNTGCACCGATACCACGACGGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAgtg                                                                       <  2:650735/300‑1 (MQ=255)                                                                                                                                                                                                                 ccGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGCCTGCCACCGAGAATGAGAACTGTCTTACCTGTCAAAGCACCCATAAAGACTCCTTGATTTATCATGTAACCTGCCTTACAACACTGCTTTCACTTTCTGTCAACAGGTTTTGTAATGGTCAATAAAAAAGAATCTAGCGTTACCGGTCGTCCCAGACGTTTCGATCCTGAACAAGCAATCTCCGCAGCAAAAGTGTTTTTTCACCACAGAGGTTTCGATGCTGTcctta                                                                       >  2:490027/1‑296 (MQ=255)                                                                                                                                                                                                                 ccGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCAACGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAgtg                                                                       >  1:300666/1‑300 (MQ=255)                                                                                                                                                                                                                 ccGCATAAGTGAATCGTACATCGGCCCCATCAGCGACTAAACAACCCACAACACCCGCACCAATACCACGACTACCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAgtg                                                                       <  2:267484/300‑1 (MQ=255)                                                                                                                                                                                                                  cGCATANGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCNGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAgtgt                                                                      <  2:713387/300‑1 (MQ=255)                                                                                                                                                                                                                  cGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTCCATGTACAAGCGCCCATAGAGAATCCTTGGTTTGTTCTGTAACGTGCATTACAAAACTGTTTTCCCTTTCTTTCAACCGGTTTTTTAATGGTCAATGAAAAACAATCTCGAGTTCCCGGTCGTCCCCGACGTTTCGATCCTGAGCAGANAATATCTGCAGCAAAAGTGCTTTTTCACCACAAAGGTTTCTATGCTGNCAgttt                                                                      >  2:707456/1‑298 (MQ=255)                                                                                                                                                                                                                  cGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAgtgt                                                                      <  1:476171/300‑1 (MQ=255)                                                                                                                                                                                                                      tAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTANAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCCATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCt                                                                  >  1:586536/1‑300 (MQ=255)                                                                                                                                                                                                                       agttaactcttaccttttccccATCGTTGACGAAACGACCTCCCATCCCTGCACCGATACCACGACTACCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTg                                                                 <  2:315162/282‑1 (MQ=255)                                                                                                                                                                                                                       aGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAACGGCACCAATACCACGGCTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTg                                                                 <  2:775267/300‑1 (MQ=255)                                                                                                                                                                                                                        ggTGAATCGTACCTTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTCAAAGCACAAATAAAGACTCCTTGATTTATTATGTAACATGCATTCCAACCCTGTTTCAACTTTCTGTCAACAGATTTTGTCGTGTTCACTAAAAAACATTCTCGCGTTCCAGGTCGTCCCGGATGTTTAGCTCATGAGTAGGCAATCCCTGCGGCAAAAGTGCGTTTTCACCACAAAGGTTTCGATGCTGTCAGTGTTGCtta                                                                >  2:743843/1‑298 (MQ=255)                                                                                                                                                                                                                        ggTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGa                                                                >  1:673867/1‑300 (MQ=255)                                                                                                                                                                                                                             aTCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCCCTAAAAAACAATCTCGCGTTACCGGTCGTCCCAGACGTTTCGCTCATGAGCAGGCAATCTCTGCGNCAAAAGTGCTTTTTCACCCAAAAGGTTTCGATGCTGTCAGTGTTGCTNGAGTTa                                                           >  2:718905/1‑300 (MQ=255)                                                                                                                                                                                                                             aTCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTa                                                           >  1:80465/1‑300 (MQ=255)                                                                                                                                                                                                                                  aCATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTCCCTGTAAAAGCGCCCATAAAGACTCCTTGATTTCTTATGTAACATGTATTACAAAACTGTTTTACCCTTCTGTCACCAGGTTTTGTAATAGTCCATCCCCCCCAACCCAGCGTTCCAGGTCGTCCAAGGCGTTTCGCTCCTGAGCAGGCAGTCTCTGTGACAAAAATACTCTTTCAACCACCCGGTTTCGATGCTGTAAGTGTTGCTGAAGTTACTgcg                                                      >  2:735184/1‑298 (MQ=255)                                                                                                                                                                                                                                  aCATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTTCTGAAGTTACTGAt                                                      >  1:431550/1‑300 (MQ=255)                                                                                                                                                                                                                                      cggccccatctgcaactaaaCGACCTACAATATCGGCACCGATACCACGACTACCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTNTGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAANAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATc                                                  <  2:698857/283‑1 (MQ=255)                                                                                                                                                                                                                                       ggCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCt                                                 >  1:340140/1‑300 (MQ=255)                                                                                                                                                                                                                                                  tGACTAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGNGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTNGCTGAAGTTACTGATTATCTTGGTATTAAcc                                      <  2:625804/300‑1 (MQ=255)                                                                                                                                                                                                                                                            acgTACGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAACCCCCCGAGCct                            >  1:88278/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 cGATAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAACCCCCCGAGCCTCTAcg                       >  1:88334/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    tAGCGGCACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAACCCCCCGAGCCTCTACGCgg                    >  1:362626/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          cACCGATACCACGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTg              >  1:692843/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     cGACTGCCACCGAGAATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTCACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCt   >  1:69596/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      ggcTGCCACCGAGAATGAGAACTGTCTTACCTGGAAAAGCGCCCATAAAGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTg  <  2:734544/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                               TGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTG  >  NC_000913/4473731‑4474307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑