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breseq version 0.39.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 27 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 689,450 | 0 | T | G | 41.7% | 35.7 / 11.6 | 37 | T478P (ACC→CCC) | lnt | apolipoprotein N‑acyltransferase |
* | NC_000913 | 3,206,480 | 0 | T | A | 36.1% | 37.5 / 12.7 | 36 | N6K (AAT→AAA) | ttdA | L(+)‑tartrate dehydratase subunit alpha |
* | NC_000913 | 3,844,219 | 0 | T | G | 33.8% | 104.8 / 12.8 | 72 | F86V (TTT→GTT) | adeD | adenine deaminase |
* | NC_000913 | 4,027,233 | 0 | T | A | 28.8% | 83.0 / 16.6 | 52 | intergenic (+5/+376) | fre/fadA | NAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase |
* | NC_000913 | 354,288 | 0 | T | G | 28.8% | 134.3 / 13.5 | 73 | V528G (GTG→GGG) | prpE | propionyl‑CoA synthetase |
* | NC_000913 | 338,069 | 0 | A | G | 28.4% | 121.9 / 15.6 | 69 | D431G (GAT→GGT) | yahF | putative acyl‑CoA synthetase YahF |
* | NC_000913 | 2,434,861 | 0 | G | T | 28.3% | 61.9 / 12.4 | 46 | P259Q (CCA→CAA) | truA | tRNA pseudouridine(38‑40) synthase |
* | NC_000913 | 2,551,293 | 0 | T | A | 27.8% | 110.2 / 19.6 | 54 | Y145F (TAT→TTT) | yfeZ | putative inner membrane protein |
* | NC_000913 | 3,003,907 | 0 | G | T | 27.8% | 77.4 / 13.7 | 54 | C140F (TGT→TTT) | xdhC | putative xanthine dehydrogenase iron‑sulfur‑binding subunit XdhC |
* | NC_000913 | 3,875,532 | 0 | A | T | 27.8% | 49.6 / 13.1 | 37 | H32L (CAT→CTT) | yidX | putative lipoprotein YidX |
* | NC_000913 | 2,014,222 | 0 | C | A | 27.4% | 90.9 / 18.5 | 62 | Q332K (CAA→AAA) | fliF | flagellar basal‑body MS‑ring and collar protein |
* | NC_000913 | 2,632,955 | 0 | A | C | 27.1% | 114.5 / 10.7 | 59 | G372G (GGT→GGG) | guaB | inosine 5'‑monophosphate dehydrogenase |
* | NC_000913 | 343,444 | 0 | A | G | 26.7% | 129.0 / 14.4 | 60 | G187G (GGA→GGG) | yahK | aldehyde reductase, NADPH‑dependent |
* | NC_000913 | 4,256,061 | 0 | C | T | 26.6% | 105.5 / 18.0 | 64 | E136K (GAA→AAA) | plsB | glycerol‑3‑phosphate 1‑O‑acyltransferase |
* | NC_000913 | 2,828,506 | 0 | T | G | 26.0% | 124.7 / 10.1 | 78 | V257G (GTG→GGG) | srlD | sorbitol‑6‑phosphate 2‑dehydrogenase |
* | NC_000913 | 3,486,386 | 0 | G | A | 25.4% | 108.9 / 12.4 | 60 | A89A (GCG→GCA) | crp | DNA‑binding transcriptional dual regulator CRP |
* | NC_000913 | 4,004,132 | 0 | G | A | 25.4% | 126.0 / 14.9 | 64 | A16V (GCT→GTT) | rarD | putative transporter RarD |
* | NC_000913 | 25,538 | 0 | A | C | 25.0% | 136.0 / 11.4 | 84 | N111T (AAC→ACC) | lspA | lipoprotein signal peptidase |
* | NC_000913 | 3,788,025 | 0 | T | G | 25.0% | 210.1 / 10.6 | 118 | G396G (GGT→GGG) | envC | murein hydrolase activator EnvC |
* | NC_000913 | 3,458,698 | 0 | C | A | 24.6% | 118.1 / 12.5 | 69 | I120I (ATC→ATA) | gspE | Type II secretion system protein GspE |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 1428794 | NA (NA) | 4 (0.060) | 4/486 | 3.1 | 7.3% | noncoding (1/1196 nt) | IS5 | repeat region |
? | NC_000913 | 3947930 = | 51 (0.750) | coding (38/840 nt) | hdfR | DNA‑binding transcriptional dual regulator HdfR |