Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,256,0610CT26.6% 105.5 / 18.0 64E136K (GAA→AAA) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (12/35);  new base T (17/0);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 3.76e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTG‑AATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTC  >  NC_000913/4255816‑4256305
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                           
aaaCAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCCCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGcc                                                                                                                                                                                                                                                        <  1:122955/251‑1 (MQ=255)
  aCAGACCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGcccc                                                                                                                                                                                                                                                      <  1:287385/251‑1 (MQ=255)
        ccTGACGAGCAGGAAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCGGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGgcgcg                                                                                                                                                                                                                                                <  1:50434/251‑1 (MQ=255)
           gACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTTACCTC‑GCCTTTTTTCCCCCCCcgcgcgcgg                                                                                                                                                                                                                                             >  1:53780/1‑250 (MQ=255)
            aCGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTT‑GCCTTTTTTACGCCCCcgcgcgcgcc                                                                                                                                                                                                                                            >  1:287828/1‑249 (MQ=255)
                       aaCGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCAcc                                                                                                                                                                                                                               <  1:153079/251‑1 (MQ=255)
                          gTGGGCCTACGGCAGCCTGACGTTGACGGCCAAAGTGCATACGCACCCCGCGCGCCAGTTTCGGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGac                                                                                                                                                                                                                            <  2:496193/251‑1 (MQ=255)
                                caccGGCAGCCAGACGTTGACGGGCAAATGGCATACGCGCCACGCGCGCCAGTTTCGGAGCGATAGTTTTATCCGTCCCGTGTT‑CACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGc                                                                                                                                                                                                                      <  1:103358/248‑1 (MQ=255)
                                  aCGGCAGCCAGACGTTGACGGGCACCGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGCGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCCGCCCGCGGCC‑CAA‑CAACACCCACACCGGccc                                                                                                                                                                                                                    >  2:551171/1‑249 (MQ=255)
                                      cggCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCATTTTCGGGGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑c                                                                                                                                                                                                               <  1:186095/249‑1 (MQ=255)
                                           aGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTC‑CCCCTTTTCCCGCCCCGGCGCGCGGCC‑AAA‑CAACACCggcccctgccccatccgccc                                                                                                                                                                                                           >  1:310263/1‑232 (MQ=255)
                                                 tGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTAAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGCCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGCTCACTTCCTCCTTTTACA‑GCCCCGTCGCGCCACC‑CAA‑CAACAACGACAATGGCCCCAT‑Ctccccacccac                                                                                                                                                                                                    >  1:66850/1‑241 (MQ=255)
                                                   aggggaaaggggCATACGCGCCCCGCGCGCCATTTTCTGAGCGATAGTTTTATCCGTGCCGGGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACTCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCGTTTTCACGCCCGGGCGCGCTACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑t                                                                                                                                                                                                <  1:551171/240‑1 (MQ=255)
                                                         aaaGTGCATCCCCGCCCCGCGCGCCAGTTTCTGAGGGATAGTTTTGTCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAACCGCCCAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CACCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGt                                                                                                                                                                                         <  1:176902/251‑1 (MQ=255)
                                                             ggCATCCGCGCCACGCGCGCCAGTTTCTGAGCGATGGTTTTAGCCGTGCCGGTTT‑CTTCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCt                                                                                                                                                                                     <  1:374401/250‑1 (MQ=255)
                                                             ggCATACGCGCCACGCGCGCCGGTTTCTGAGCGAGAGTTTGACCCGTGCGTTTTT‑CTTCCGCCATCCGGCGCAGCGAACCTGACGGCGAAAAAGGCACAAAACTGTCGTCACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGGGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCt                                                                                                                                                                                     <  1:484269/250‑1 (MQ=255)
                                                               cATACGCGCCCCGCGCGCCAGTTTCTGAGGGATAGTTTTATCGGGGCCGTGTC‑CACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAACCGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTAc                                                                                                                                                                                   <  1:187493/251‑1 (MQ=255)
                                                                atccgcgCCACGCGCGCCAGTTTCGGAGCGATAGTTGTATCCGGGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTGGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACg                                                                                                                                                                                  <  1:385092/248‑1 (MQ=255)
                                                                           gcgcgcCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTC‑GCCTTTTTTACGCCCCCGCGCGCGGCC‑CAA‑CATCCCCCACACCGGCCCCAT‑CTGCACCTCCCG‑A‑TT‑TTGGTTGCTACGGTGCGAAtagg                                                                                                                                                                       >  1:212871/1‑248 (MQ=255)
                                                                                ccAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTT‑GCCTTTTTCACGCCCCCGCGCGCGACC‑AAA‑AATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TTGGTTGCTACGGTGCAAATCGAGAt                                                                                                                                                                    >  1:135517/1‑249 (MQ=255)
                                                                                  aGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTTACCCCCCCGCGCGCGGCC‑AAA‑CATCACCGACACCTGCACCCT‑CTGCCCATCCCG‑A‑TT‑TTGGTTGCTACGGTGGCAATCGGGATAGTc                                                                                                                                                                >  1:583860/1‑251 (MQ=255)
                                                                                  aGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTTACTTC‑GCCTTTTTTTCGCCCCGGCGCGCGCCC‑AAA‑AAACACCCACAATGGCACCAT‑CTTCCCATCCAG‑A‑TT‑TTGGGTGCTACGGGGCAAAACGAGGTAGtg                                                                                                                                                                >  1:565095/1‑250 (MQ=255)
                                                                                        tGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTTCCTTC‑CCCTTTTTTACCCCCCCCCGCGCGACC‑AAAACA‑CCCCCCCCCTGGCACCCT‑CCCCCCATCCCA‑A‑TT‑TTGGGTGCTACGGTGCAAATCGAGATAGTCggggg                                                                                                                                                           >  1:398983/1‑246 (MQ=255)
                                                                                             gagatttttACCCGTGCC‑TGTTTCATCCGCCATACGGCCCAGCGAACCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCt                                                                                                                                                     <  1:496875/246‑1 (MQ=255)
                                                                                                aagttttaccctgcccgtttcaacccGCCATACGGCGCAGCGAAACTGACGGCGAAAAACGCAAAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTa                                                                                                                                                   <  2:565095/227‑1 (MQ=255)
                                                                                                aGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCCTTTTCACGCCCCCGCGCGCGACC‑CAA‑CATCACCGACACCGGCACCCT‑CTGCCCCTCCCG‑A‑TT‑TGGGTTTCTACGGAGCACAACGCGATAGTCGTGGGACAGCTTa                                                                                                                                                   >  1:284304/1‑250 (MQ=255)
                                                                                                aGTTTTACCCGGGCCGTTTT‑CACCCGCCATACGGCGCAGGGAAACGGACGGCGAGAAACGCAAAAAACTGGCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTa                                                                                                                                                   <  2:267584/250‑1 (MQ=255)
                                                                                                      aTCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACGCTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAc                                                                                                                                             <  1:404929/250‑1 (MQ=255)
                                                                                                        ccttgccgtgGC‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACGGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACA‑AAC‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCGACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGActct                                                                                                                                          <  1:64880/248‑1 (MQ=255)
                                                                                                          gtgccgtgTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGCTTAACTTC‑GCCTTTTTTACGCCCCCGCCCGCGACC‑CCA‑CAACACCGCCACTGGGCCCAT‑CTGCACATCCAG‑A‑TT‑TGGGCTACTACGGTGCAAATAGAGATAATCATGGAACAACTTAATACACtcttt                                                                                                                                        >  1:239357/1‑250 (MQ=255)
                                                                                                          gggccttgtT‑CACCCGCCATAAGGCGCAGCGAAACTGCCGGCGAGAACCCCACAAAACTGTGGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCAGACGCAGCGGCGGGTTCACTTC‑GCCTTTGTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttc                                                                                                                                        <  2:397006/245‑1 (MQ=255)
                                                                                                            gccgtgTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACCCCCCGGCGCGCGGCC‑AAA‑AATCACCCAACCTGGCACCAT‑CTGCACAACCAG‑A‑AT‑TGGGTTGCTACGGTGGAAATCGCGGAAGGCGTGGGACAGCTTAATAGACTcttctt                                                                                                                                      >  1:459475/1‑251 (MQ=255)
                                                                                                              cgtgTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTc                                                                                                                                    <  1:373935/251‑1 (MQ=255)
                                                                                                               gtgtT‑CATCACCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAACCTGTCGCGACCGAGCCACCGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTC‑GCCCTTTTTTCGCCCCCGCGCGCGGCC‑AAA‑CCTCACCGACACCGGCACCCC‑CCGCACACCCCC‑A‑TT‑TGGGTTGGTACGGTGCACATCGAGATAGTCGTggggccgcgtaaatgaatcttcttttt                                                                                                                                   >  2:528572/1‑226 (MQ=255)
                                                                                                                     cATCCCCCAAACGGCGCAGCAAAACGAACGGCGAGAAACCCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCCCTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCgtg                                                                                                                              <  2:53780/251‑1 (MQ=255)
                                                                                                                       cccGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTa                                                                                                                            <  1:198298/250‑1 (MQ=255)
                                                                                                                        ccgccagccGGCGCAGCGAAACGGACGGCGAGAAACGCACAAAACTGTCGCGACCAAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCgtgt                                                                                                                             <  2:100429/241‑1 (MQ=255)
                                                                                                                          gCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCCTTTTTACGCCCCGGCGCGCGACC‑AAA‑AATCACCCCCAACGGCACCAT‑CTTCACATCCAG‑A‑TT‑TGGGTTGCTACCGGGCAAAACGAGATAGTCGGGGAACAGCTTAATACACTCTTCTTTTGGCGTGGAAt                                                                                                                          >  1:399114/1‑250 (MQ=255)
                                                                                                                            cATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAgg                                                                                                                       <  1:572852/251‑1 (MQ=255)
                                                                                                                                cGGCGCAGCGAAACTGACGGCGAGAAACGCACAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGaa                                                                                                                   <  1:13796/251‑1 (MQ=255)
                                                                                                                                      aGCGAAACGGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACAcg                                                                                                             <  1:425904/251‑1 (MQ=255)
                                                                                                                                          aaaCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTTACTTC‑GCCTTTTTTACGCCCCCGCGCGCGACC‑AAA‑CATCCCCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TTGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAAAAGAATCTTCTTTTTGCGTGTAATAGGGGAACACACCCGGc                                                                                                         >  1:324888/1‑251 (MQ=255)
                                                                                                                                               gACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCccgcc                                                                                                    <  1:442680/251‑1 (MQ=255)
                                                                                                                                               gACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCCCTTC‑GCCCTTTTTTCTCCCCCGCGCGCGGCC‑AAA‑CAACACCCACACCTGCCCCCT‑CTGCACAACCAG‑A‑TT‑TGGGGTGCTACGGTGGAAAAAGAGATAGGTGTGGGAAAGATTAATAGACTCTTTTTTTGGCGGGTAATAGGGGAAAAAACGCGGCccgcc                                                                                                    >  2:442680/1‑251 (MQ=255)
                                                                                                                                                            gCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTTAATTC‑GCCTTTTTTACCCCCCCGCCCGCGACCCAAA‑AACCAC‑GACACTCGCCCCCT‑CTTCACAACCCGCA‑TT‑TGGGT‑GCTACGGGGCAAATCGCGGTAGGCGTGGGACAGCTCTATAGACTCTTCTTTTGGCGGGGAATAGGGGAACACACGCCGCCCGCCGTG‑AATGAacac                                                                                       >  2:427243/1‑250 (MQ=255)
                                                                                                                                                               caAAACTGTCGCGACCGAGCCACAGTACCGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑CCCTTTTTTACCCCCCCGCGCGCGACC‑AAA‑CATAACCCACACTGGCACCCT‑CTGCACAACCCG‑ACTT‑TGGGT‑GCTACGGGGCCAATCCAAGTAATCGTGGAACAGCTTAATAGACTCTTCTTTTGGCGTGTAATAGGGGGAAACACCCGGcccccccgggatgaacaaaca                                                                                     >  1:145663/1‑232 (MQ=255)
                                                                                                                                                                aaaaCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTTCCGCCCCCGCGCGCGACC‑AAA‑CATCCCCGACACTGGCCCCCT‑CTTCACATCCCG‑A‑TT‑TTGGTTGCTACGGGGCAAATCGAGGTAGTCGTGGGACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGGGAACACACGCGGCCCCCCCTG‑AATGAACACATAgc                                                                                  >  1:114447/1‑251 (MQ=255)
                                                                                                                                                                  acctgccgccaCCGCGCCACATTACAGCAAAAAATTTCTGTCCCCCGTAAGGCATCCCCGGGGGCGGTTCCCCTTC‑CCCTTTTCCCCGCCCCGCCGCGC‑ACC‑AAAACATCCCCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTAAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGGGGCCCGCCGTG‑AATGAACACATAgcgc                                                                                <  1:6234/241‑1 (MQ=255)
                                                                                                                                                                      gTCGCCACCGAACCACAGTACCGCGAAAAATTTCTTTACGCCGTTAAGCAAACGGAGCGGGGGGTTCACTTC‑GCCTTTTCCACCCCCCGGCGCGCGACA‑AAC‑CATCACCGACACTGGCCCCAT‑CTGCCCATCCAG‑A‑TT‑TGGGTTGCTACGGGGAAAATCGAGATAGTCGTGGAACAGTTTAAAAGCCTCTTCTTTCGGCGTGTAATAGGTGAACAAACGCGGCCCGCCGTG‑AATGAACACATAGCGCGGc                                                                             <  1:320425/250‑1 (MQ=255)
                                                                                                                                                                         gcgACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTTACGCCCCCGCGCGCGACC‑AAA‑AATCACCGACACTGGCACCAT‑CTTCACATCCAG‑A‑TT‑TGGGGTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAATCTTCTTTTTGCGGGTAATAGGGGAACACACGCGGCCCGCCGGG‑AATGAAAACATAGCGCGGCAGt                                                                          >  1:226153/1‑250 (MQ=255)
                                                                                                                                                                         gcgACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTC‑GCCTTTTTTACGCCCCGGCGCGCCACC‑CAA‑CATCACCCACACTGGCACCAT‑CTGCAAATCCAG‑A‑TT‑TGGGGTGCTACGGGGCAAAACAAGATAGTCGGGGAACAGCCTAATAAACTCTTCTTTCCGCGGGTAATAGGTGAAACCACACCGCCCGCCGGG‑AATGAAAACATAGCGCGGccgt                                                                          >  1:295794/1‑247 (MQ=255)
                                                                                                                                                                                     caGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCAATTC‑GCCTTTTTTACGCCCCCGCGCCCGGCC‑AAA‑AATCAACCACACCGGCACCAT‑CTGCCCCTCCCG‑A‑TT‑TGGGTTGCTCAGGTGCAAAACGCGGTAGTCGGGGGACAGCTTTATAAACTCTTCTTTTTTCGTGTAATAGGTGAACACACGCGGCCCGCCCTGGAAT‑AACACATAACGAGGCaagcacgtgccgtccg                                                             >  2:577144/1‑236 (MQ=255)
                                                                                                                                                                                                aaaaTTTCTGTCCGCCTTAAAGCTTGCGCGGCGGGGGGTTCATTGC‑GCCTTTTTCCCGCCCGGGCGCGCGACC‑AAC‑CACCCCCGACACTGGCACCTT‑CTGCACATCCA‑‑A‑TTGTGGGTTGCGGCGGTACAAATCGAGATTGTCGTGGAAGAGCTTAAAAAGCTCTTCTTGCGGAGTGTAATGGGGAAACACACGCGGGCCGCCGTG‑AATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGc                                                  <  1:321642/251‑1 (MQ=255)
                                                                                                                                                                                                                  taagcaaccgcggcgggggtttcccTTC‑GCCTTTTTCCCCCCCCGGCGCGCGACA‑AAA‑CATCACCGACACGGGCACCAT‑CTGCACATCCAG‑T‑TT‑TGGGTTGCTAGGGTGCAAATCGAGATAGTCGTGAAACAGCTAAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACCCCCGCGGCCCGCCGTG‑AATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCaa                                 <  1:104311/226‑1 (MQ=255)
                                                                                                                                                                                                                   aaGCATCCGCGGCGGCGGTTTCCCTTC‑CCCTTTTTCCCCCCCCTGCCGGCGACC‑AAA‑CACCACCGCCGCTGCCCCCAT‑CTGCACATCCAG‑A‑TT‑GGGGTTGCTCCGGTTCAAATCGAGATGGTCGTGTAACAGCTTAATAGCCGTTTCTTTCGGCGTGTAATATGTGAGCACACGCGGCCCGCCGTG‑AATGAACACATTGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAg                                <  1:383758/250‑1 (MQ=255)
                                                                                                                                                                                                                                ggggggTTCCCTCC‑GCTTTTTTCCCGCCCGGGCGCGCGACC‑AAA‑CTTCACCGACACTGGC‑CCATTCTGCACATCCAG‑A‑TT‑GGGGTTGATACGGTGCAAATCGAGATAGTCGTGAAACAGTTTAATAGTCTCTTCTTTCGGCGTTTAATAGGGGAACCCACGCGGCCCGCCGTG‑AATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGaca                  <  1:311985/248‑1 (MQ=255)
                                                                                                                                                                                                                                     gTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTG‑AATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTggg             >  1:172518/1‑251 (MQ=255)
                                                                                                                                                                                                                                        cACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTG‑AATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcgc          >  1:140438/1‑251 (MQ=255)
                                                                                                                                                                                                                                         cccttcccttttttCACCCCCCGGCCCGCGACC‑AAA‑CATCCCCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTG‑AATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcgc          <  1:471903/242‑1 (MQ=255)
                                                                                                                                                                                                                                         ccctcctcttttttCACCCCCCGGCGCGCCACC‑AAA‑CATCACCGCCACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGTGTTGCTACGGTGCAAATGGAGATAGTCGTATAACAGCTTAATAGCCTCTTTTTTCGGTGTGAAATGGGTAAACACCCGCGCCCCGCCGTG‑AATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcgc          <  1:285032/242‑1 (MQ=255)
                                                                                                                                                                                                                                         aCTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTG‑AATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcgcg         >  1:232147/1‑251 (MQ=255)
                                                                                                                                                                                                                                               gCCTTTTTCACGCCCCGGCGCAAGACC‑AAA‑CATCACCGAAACAGGCACCAT‑CTGCACATCCCG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTGGTGGAACAGCTTACTAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTG‑AATAAAAACATAGCGCGGCAGCAGCGTGCCGTCGATTTCCAGCGGCTCTACCGGGTCCGGACAGTCATGTGCCAGACACTGGGCGCGCAACg    >  2:525951/1‑251 (MQ=255)
                                                                                                                                                                                                                                                ccTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGAGTGTAATAGGTGAACACACGCGGCCCGCCGTG‑AATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGt   >  1:570927/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 cTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTG‑AATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACCGGTCAGGCAAGTCATGTTCCAGACACTGGTCGCGCAACGTc  >  1:319808/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                           
AAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTT‑CATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTC‑GCCTTTTTCACGCCCCGGCGCGCGACC‑AAA‑CATCACCGACACTGGCACCAT‑CTGCACATCCAG‑A‑TT‑TGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTG‑AATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTC  >  NC_000913/4255816‑4256305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑