| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 338,069 | 0 | A | G | 28.4% | 121.9 / 15.6 | 69 | D431G (GAT→GGT) | yahF | putative acyl‑CoA synthetase YahF |
| Reads supporting (aligned to +/- strand): ref base A (8/40); new base G (19/0); total (29/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.19e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGG > NC_000913/337820‑338313 | gcgtcggcACGCTGTGCAAGGAAACCATGTCCGCGGGGATGGAAAAACATGGCGATGCCTACACCAACATTCAGCCCGACCCGGAATTCCACCTGAAAGATATCAACCGCAGCATCAAACCCACCTTCCGCGACTTTGGCGATGACGTCTTCACCAATGGCAAGCCGCACCCGATGATTGCCCCCACCAACCGCATCAGTCGCTTGATCGAAGAGTCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGAt < 2:142724/247‑1 (MQ=255) cggcggCACGCGGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGAtt < 2:426924/251‑1 (MQ=255) aCGCTGTGCGACGAAACCATGGTCGCGGTGATGGAAAAACATGGCGGTGTCTACAGCAGCATTCAGCCCGATCCGGAATTCCGCCGGAAAGATATCAACCGCAGCATCAAACACACCTGCCGCGACTGTGGCGATGACGACTTCACCAATGGCAATCCGCACCCGGTGATTGACGCCACCAACCGGATCAGTCGCTTTAGCTATGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑Ct < 1:306350/251‑1 (MQ=255) cGCTGTGCAACGACACCATCTTCGCGGTGATGTAAAAACATGGGTATGCCTCCAGCAATATTCAGCCCGATCCGGAATTCCGCCTGAAATTTATCAACCGACGCACCAATCACCCCTGCCTCGGCTTTGGCGATGACGACTTCTCCAATGGCAAGCCGCACCCGATGAGTGACCCCACCAACCGCATCAGTCGCTTGATCGAATAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑Cta < 2:257188/251‑2 (MQ=255) cgaAACCATGGTCGCGGTGATGGAAAAACATGGCGTTGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCGTGAAAGATATCAACCGCAGCATCAAACACGGCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCTCCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATC‑TGTATGGAATTTGTG‑CGC‑GGA‑TTTGGAt < 1:471338/251‑1 (MQ=255) aCCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCGTG‑ATGGGAGTTGTG‑CTC‑GGA‑TTTGGAtagc > 1:404128/1‑247 (MQ=255) aTGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGGTCGTG‑GTGGGTTTTGTG‑Cttggggttgggtcgcgacg > 2:196864/1‑233 (MQ=255) tCGCGGTTATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑a < 2:494491/250‑1 (MQ=255) cgcgGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGGTCGGG‑ATGGGGTTTTTG‑GTC‑GGG‑GTTGGATCG‑CATGa > 1:180753/1‑246 (MQ=255) gcgGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATcc < 1:351772/251‑1 (MQ=255) gATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTc < 1:10577/250‑1 (MQ=255) aaaaCATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGGTTTTGTT‑GTC‑GGG‑TTTTGATCG‑CATGAA‑G‑ATCCGGGCGGCTccc > 1:502207/1‑250 (MQ=255) cattgCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCTCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTGGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCAt < 1:60751/247‑1 (MQ=255) gTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGAGCGTG‑ATGGGTTTTTTG‑CGC‑GGA‑TTTGGATCG‑GATGAA‑G‑ATCCGGTCGGGTCCCCCCTCGCGACggt > 1:421486/1‑249 (MQ=255) cAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGGTCGTG‑ATGGGTTTTTTG‑CTCTGGT‑TTTGG‑TCG‑CCTGAC‑A‑CTCCGGTCGGCTCCCCCATCGCGACgcaccaagaa > 2:430410/1‑241 (MQ=255) aaCATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGGTTTTTTG‑GTC‑GGG‑TTTTGGTCG‑CCTGAA‑G‑ATCCGGGCGGGTCCCCCCTCGAGACGATCaaaaaa > 1:457953/1‑246 (MQ=255) aaCATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGaagc < 1:439206/251‑1 (MQ=255) aaCATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGGG‑ATGGGATTTGTG‑CTC‑GGG‑GTTGGATCG‑CATGAA‑G‑ATCCGGGCGGCTCCCCCCCCTAGACGATCCaagaa > 1:423962/1‑249 (MQ=255) ccccGATCCGAAATTCGTCCTGAAAGAAATCACCCGCAGCATCAAACACACCTTCCTCGACTTTGGCGAGGACGACTTCACCAATGGCAAGCCGCACCCGATGAGTGACCCCACCAACCGCATCAGACGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCGTG‑ATGGATTTTTTG‑CTC‑GGA‑ATTGTATCG‑CATGAA‑A‑ATCCGGTCGGCTCCTCTAGCTATACGATCAAAGaagcgaaagcga < 2:549989/250‑1 (MQ=255) ccGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGTCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATc < 1:261488/251‑1 (MQ=255) cgtaattcccgttgaatgatttaacccGCAGCGACAACCACCCCTTCCTCGACTTTGTCGAAGATGACTTTCCCAATGTCAAGCCGCACCCGATGATGGACCCCACCAACCGCATCAGTCGCTTTATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CAC‑GTA‑TTTGGACCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCt < 2:9023/226‑1 (MQ=255) ggAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGGTCGTG‑GTGGGTTTTTTG‑CTC‑GGAGTTTGG‑TCGGCA‑GAA‑GGATC‑GGTGGGCTCCACCATCCAGACGGTCAAAAAAGCGAAAACGAAcgccgccc > 1:124617/1‑249 (MQ=255) cGCCTGAAAGAAATCAACCGCAGCATCAAACACACCTTCCGCGACTTTGGCGATGACGACTTCACCAATGTCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCGTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCGCCACCATCGAGACTATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAgg < 2:349965/251‑1 (MQ=255) gAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGAcgc < 1:196864/250‑1 (MQ=255) atCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGa < 1:374641/250‑1 (MQ=255) cAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATc < 1:140089/250‑1 (MQ=255) cGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGCGGTCGTG‑AGGGGTTTTGTG‑CTC‑GGG‑TTTTGGTGG‑CGTGAA‑G‑ATCCGGGGGGGCCCCCCCTCGCGGCGATCAAAAAAGCGAAAGAGGTCGCCGCCGCC‑GGAgggggcggggggttgctttt > 2:90602/1‑233 (MQ=255) cagcaTCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGGG‑GTGGGTTTTTTT‑CTC‑GGG‑TTTGGGTCG‑CATGAA‑A‑ATCCGGGCGGCTCCACCATCGCGACCGTCAAAAAAACGAAAACGATCGCCGCTGCC‑GAAGGACGCGCGGTTATCATtctcc > 1:214021/1‑250 (MQ=255) aaaCACACCTTCCTAGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGCAGCGGCGCGCGATCCAGAAGTGGCGGAGCTCGGG‑GGGGTGTTTGTG‑GTC‑TGG‑TTTTGATTG‑CATGAA‑G‑AACCGGGCGGCTCCTCCCTCGCGACGCACATAGAAGAGAAAGGGATGGCCGCCGCC‑CCAGGAcgcgggtggtttcttatcgccgcatg > 2:92872/1‑229 (MQ=255) aaCACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGAGCTTG‑GTGGGTTTTTTG‑CGC‑GGG‑TTTGGATCG‑CATGAA‑G‑CTACGGGTTCCTCCCCCAACGCGACCAGCAAAGAAGCGGAAGCGATCGCCGCTTCC‑CCAGGACCCGCGGTGATCATTTTCACCTCtgtg > 1:162404/1‑251 (MQ=255) cacCTTCCTCGACTTTGGCTATGTCGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCTCCAACCGCATCATTCGCTTGATCGAAGAGGCGCGCGATCCATAAGTGGCGGTGATCGTG‑ATGGATGTTGGG‑CTG‑GGA‑TTTGGTTCG‑CTTGTA‑G‑TGCCGTTCTTCTCCTCCATCGTGACGATCAAAGAAGCGAAAGCGTTCGCGGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCt < 1:561403/249‑1 (MQ=255) cccTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTggg < 1:90602/250‑1 (MQ=255) cTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGGTTTTGTG‑CTC‑GGG‑TTTGGGTCG‑CATGAA‑A‑ATCCGGGCGGGTCCCCCCTCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGGGAGGTGATCATTCTCGCCTATGTGCTggggg > 1:396438/1‑249 (MQ=255) ccTCGACTTTGGAGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGGTCGTG‑ATGGATTTTTTG‑CTC‑GGG‑TTTTGATAG‑CATGAAAG‑A‑CCGGTCGGCTCCACCATCGAGACGATCAAAAAAAAGAAAAAGGGCGCCGCTGGC‑GCAGGACGCGGGGTGGTCATTCTCCCCCATGTGTTgggggccc > 1:129662/1‑246 (MQ=255) cGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGaagcgaa < 2:322597/189‑1 (MQ=255) gACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCt < 1:63030/251‑1 (MQ=255) cGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTGGATACGc < 2:246766/251‑1 (MQ=255) acgacTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCg < 1:74605/251‑1 (MQ=255) tCACCAATGGCAAGCCGCACCCGATTATTGACCCCACCAACCGCATCAGTCTCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATGTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑GTCCGGTCGTCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTTATCATTCTCTCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAg < 1:240557/249‑1 (MQ=255) cACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAATCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCGATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGaa < 1:538468/250‑1 (MQ=255) aTGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGCAGTGGCGGAGATCGTG‑GTGGGTTTTTTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCCGTCGGCTCCACCATCGAGAAGATCAAAAAAGCGAAAACGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTCTGTGCTGGGGGCCGATCTTGATACGCCATCGTTAGAacaaca > 1:267241/1‑250 (MQ=255) gggCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACaaa < 2:457953/250‑1 (MQ=255) ggCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAg < 1:564442/251‑1 (MQ=255) gCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGGG‑AGGGGATTTGTG‑CTC‑GGT‑TTTGGGTCG‑CCTGAA‑G‑ATCCGGGGCGCTCCACCATCGAGACGACAAAAAAAGCGAAAGCGAACGCCGCTGCCCGAAG‑ACGCGAGGTGGTCATTCTCGCCTTTGTGTTGGGTCCCGACTTTGGTAACCCATCGTTAGAACAAAAAAACCAGAtgc > 1:189146/1‑251 (MQ=255) gatTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCCCGCGATCCAGAAGTGGCGGTGATCGTG‑CTGGGTTTTTTG‑CTT‑GGA‑CTTTGCTCC‑CATGAA‑GCATC‑GGTCGGCTCCCCCCACCAGACGATCCAAAAAGCGCAAGCGAACGCCGCTGGC‑GAAGGACGCGAGGTGATAATTCTCCCCCCTTTGCTGGGTACCGACCTTCATACGCCATTGCTTGCACAACAACACCAGATGCTGCTTgttg > 1:547204/1‑248 (MQ=255) atTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGc < 1:32157/251‑1 (MQ=255) atTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGGG‑ATGGATTTTTTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGGCGGCCCCACCATCCAGACGGTCAAAAAAGCGGAAGCGGACGCCGCCGCC‑GAAGGACGCGAGTTGATCATTTTCGCCTCTTTGCTGGGGACCGGTCTTGATACGCCATCGTTAGAAAAACAAAACCAGAAGGCGCTTGATGc > 1:221989/1‑251 (MQ=255) tGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGAGCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTGGGATCG‑CATGAA‑T‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGcc < 2:404224/250‑1 (MQ=255) accaACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGAGATCGTG‑GTGGGTTTTGTG‑CTC‑CGG‑TTTGGGTCG‑CCTGAA‑G‑ATCCGGGCGGCTCCACCATCGAGACGAGCAAAAAAGCGGAAGCGATCGCCGCTGCC‑GCAGGAAGCGAGGTGATAATTCTCGCCTCTGTGCTGGGTACCGCGCTTGATACGCCACCGTTTGAACAACAAAAACAGATACTGCTTGGTGCCGGCGTggt > 2:446265/1‑249 (MQ=255) gCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGACATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGagcagcagc < 1:307355/251‑1 (MQ=255) tGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAgcagcagca < 2:421486/249‑1 (MQ=255) cGAAGAGGCGCGCGATCCAGAAGTGGCGGTGGTCGGG‑ATGGGTTTTGTT‑CTC‑GGG‑TTTTGGTTG‑CCTGAA‑G‑ATCCGGGCGGGCCCCCCCCCGCGACGGTCAAAAAAGCGAAAGCGATCGCCGCTGCC‑GCAGGAAGCGGGGTGATCACTTTCGCCCATGTGCTTGGTACCGCTCTTTATACGCCATCGTTTGAACAAAAAAAACAGCTGCGGCTTGCTGCCGGGGTGGTTCTGGCGAGCAACAGCCCCaac > 2:165455/1‑249 (MQ=255) agGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGGTTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCCTCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCCCCTATGTGCTGGGGACCGGTCTTGATACGCCATCGTTAGAACAACAAAACCAGATGCTGGTTGATGCCGGAGGGATTCTGGCGAGCAGCAGCACCCATACCggg > 1:157781/1‑250 (MQ=255) ggCGCGCGATCCAGAAGGGGCGGTGATCGTG‑ATGGATTT‑GTGGCTC‑GGA‑TTTGGATCG‑CAGGAA‑G‑TTCCGGTCGGCTCCACCAACGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGAt < 2:505512/251‑1 (MQ=255) gcgcgcGGTCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGAtt < 2:178602/251‑1 (MQ=255) gATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGGG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTTATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGgc < 2:283158/251‑1 (MQ=255) ggCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACAATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGAAttt < 2:218948/250‑1 (MQ=255) cGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATc > 2:32404/1‑251 (MQ=255) cGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATc < 1:547688/251‑1 (MQ=255) cGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCAGGTCCGCTCCACCATCGAGACGATCAAAGAAGCGCAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGCTCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATc > 1:345339/1‑251 (MQ=255) ggTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCt < 1:336758/251‑1 (MQ=255) ggTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGATCCACCATCGAGACGATCAAAGAATCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGAGCTGGGTACCGATCTTGATACGCCATCGTTAGAACCACAAAGCCAGCTGCTGCTTGATGCCGGAGTGATTCTGGCGAGCCGCAGCCCCAATACCGGATTGCTGGGGCGTGAATTTctct > 2:166662/1‑251 (MQ=255) gTGATCGTG‑ATGGTTTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTg > 1:127707/1‑251 (MQ=255) gTGATCGAG‑ATGGATTTTGTG‑CTC‑GGC‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTCTCTg > 1:499575/1‑251 (MQ=255) gATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCa < 2:502207/251‑1 (MQ=255) gATCGTG‑ATGGATTTTGTG‑CTA‑GGA‑TTTGGATAG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCAGGAGTGATTCTGGCGAGCAGCAGCACCACTACCGGATTGGTGGCGAGTGAATTTATCTGc > 2:426834/1‑250 (MQ=255) cGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAg < 1:528397/251‑1 (MQ=255) gTG‑ATGGATTTTGTG‑ATC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGGGAATTTATCTGCaacgg > 2:249800/1‑248 (MQ=255) gg‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGAGCTTGAGACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGATTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAggg < 1:524095/250‑1 (MQ=255) | GCGGCGGCACGCTGTGCGACGAAACCATGTTCGCGGTGATGGAAAAACATGGCGATGTCTACAGCAACATTCAGCCCGATCCGGAATTCCGCCTGAAAGATATCAACCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTG‑ATGGATTTTGTG‑CTC‑GGA‑TTTGGATCG‑CATGAA‑G‑ATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC‑GAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGG > NC_000913/337820‑338313 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |