| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,828,506 | 0 | T | G | 26.0% | 124.7 / 10.1 | 78 | V257G (GTG→GGG) | srlD | sorbitol‑6‑phosphate 2‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (15/42); new base G (18/2); total (34/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.91e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGGTTGGCGTGGGCCG > NC_000913/2828257‑2828751 | aGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTg < 1:151364/251‑1 (MQ=255)aGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTAGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATTCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTg < 1:361809/251‑1 (MQ=255) ggCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTTCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCTTGTG‑ATGtt < 2:559499/251‑1 (MQ=255) ggCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGtt < 2:123750/251‑1 (MQ=255) gcctaaCGGTGCATTCACTGATGCTCGTGAACCTTCTGAAATCGCCGATGTGCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCAGATCAAGTCGAGCAGTATTACATCGACAAATTACCGCTCAAACGCGGCTTCGAATATCAAGACTTGCTGAATATGCTGCTGTTCTCCGCCAGTCCTAATGCGTCTTACTGCACCGGTCAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGtta < 2:145873/246‑2 (MQ=255) aCGGTGCATTCACTGATGCTCGGTAACCTTCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAATATGTGCTGAATATGCTGCTGTTCTACTCCAGTCCTAAGGCGTCGTACTGCACCGTACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGAt < 1:592071/250‑1 (MQ=255) gCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCATGTG‑ATGTTCTgttccac > 1:15651/1‑243 (MQ=255) cATTCACTGATTCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑c < 1:281535/251‑1 (MQ=255) aTTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTTTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGGCAGTCCTAAGTCGTCGTACTGCACCGGGCAGTCGATCAAGGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑Cg < 1:261253/251‑1 (MQ=255) tCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑Cgg < 1:457772/250‑1 (MQ=255) tCACGGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGAGCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGAGCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑Cgg < 2:499847/250‑1 (MQ=255) cTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCCGGGG‑ATGTTCTGGTCAACAG‑Cgggggg > 2:184056/1‑247 (MQ=255) cTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGG‑ATGTTCTGATCAACAG‑Cgg > 1:15166/1‑247 (MQ=255) gATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCCGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGG‑GGGGTCTTATCAACAG‑Cgggggatc > 2:393052/1‑245 (MQ=255) gATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGG‑GTGGTCTGATCAACAG‑CGGGGATcc > 2:121805/1‑251 (MQ=255) aTGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGCCCGGGG‑GTGTTCTGTTCAACCG‑CGgagagccc > 2:188646/1‑247 (MQ=255) gCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCAtt < 2:15166/251‑1 (MQ=255) cGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGG‑ATGTTCTGATCAACAG‑CGGAGATCCATTa > 1:96599/1‑249 (MQ=255) gTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAATAG‑CGgagacccctttaggg > 1:130674/1‑240 (MQ=255) aaCCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGTTG‑ATGTTCTGATCAACTG‑CGGAGATCCATTAAgggt < 2:301102/250‑3 (MQ=255) aCCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGAtct < 2:315791/251‑1 (MQ=255) aCCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGAtct < 1:251422/251‑1 (MQ=255) gAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTg < 1:143562/249‑1 (MQ=255) aaaTCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGG‑ATGTTCTGATCAACAG‑CGGCGATCCCTTAAGGGTCTCCGTgggg > 1:278544/1‑248 (MQ=255) gCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGAAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGCG‑ATGTTTTGATTAAAAGGCGG‑GATCCCTTTAGGATCTCCGTGAGAatat > 1:594430/1‑250 (MQ=255) gTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATg < 1:66847/250‑1 (MQ=255) caggcACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGa < 2:549377/247‑1 (MQ=255) gTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGG‑CGGTTTTGGTCAACAG‑CGGAGATCCATTAAGGACATCCGTGCGACTATAGAAAGCCTGATGCGCtcc > 2:904/1‑249 (MQ=255) gCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGGCAGGGG‑AAGTTCTGATAAACAG‑CGGAGAGCCATTCAGGGTCTCCGTGGGACTATAGAATGCCTGATGCGCCACGcc > 2:574601/1‑250 (MQ=255) acaATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCGGATGCGCTACGCtcat < 1:184056/251‑1 (MQ=255) caATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCAt < 1:106820/204‑1 (MQ=255) caATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCtcatc > 1:411707/1‑251 (MQ=255) caATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCtcatc < 1:218431/251‑1 (MQ=255) aaTACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCTGGTG‑CTGTTTCGATCAACAG‑CCGAGGGCCATTAAGGGTCTCCGGGGGGCTATAGAATTACTGATGGGCTTCGCtcctc > 2:56266/1‑247 (MQ=255) cgACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCCTTAAGGATCTCCGTGAGACTATAGAATGCCTTATGCGCTACGCTCATCAGGCAt > 1:111262/1‑250 (MQ=255) cgACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATa < 1:336228/251‑1 (MQ=255) aCCAAGCTGGGTATCAAACCGGCTCAAGTCGAGCAGTATTACATCGACCAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGG‑TTGTTCTGATCAACAG‑CGGAGAGCCATTAAGGGTCTCCGAGAGACTATAGAATGCCTGATGCGCTCCCCTCATCAGGCATAc > 1:476215/1‑250 (MQ=255) gCTGGGTATCAAACCGGATCAAGTCGACCAGTATTACATAGAACAAGTACCGCTCAAACGCGGATGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCCTTAAGGATCTCCGTGGGGCTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACt > 1:214957/1‑251 (MQ=255) tATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGG‑ATGTTTTGATCAACAG‑CGGGGATCCATTAAAGATCTCCGGGAGAATCTAGAATGCCTGATGCGACAAGCTCATCAAGCATACAGGAATTCCGcc > 1:500837/1‑251 (MQ=255) gATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTc < 1:364542/153‑1 (MQ=255) ttACATCGAAAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTTTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGAATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTAt < 1:121805/250‑1 (MQ=255) ccATCGACAAAGTCCCGCTCAACCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGTTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGGGGTCGGGTT‑ATTTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCCCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATcc < 1:394485/249‑1 (MQ=255) acaacGACAAAGTACCGCTCAAGCGCGTTTGCGATTACCAAGAGGTGGTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTACACCGGACAGCCAATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAGGGATCTCCGTGAGACTATAGAATGCCTGATGCCCTACGCTCATCGGGCATACAGAACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCg < 1:5976/247‑1 (MQ=255) aCATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACCACATTAAGGAAAAGTTATGGGATccc > 1:206789/1‑250 (MQ=255) gACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGG‑ATGTTCTGATCAACAG‑CGGAGATCCCTTAAGGATCTCCGTGAGACTATAGAATGCCTGACGCGCTACGCTCATCAGGCATACAGGACTTCCGCCCCTACATTAAGGAAAAGTTATGGGATCCGCACtc > 1:437123/1‑251 (MQ=255) aaaaaattACCGCGCAAACGCGGCTGCGTTTACCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCAAGGGCGGCGTACTGCACCGGACAGTCGATAAATGTCCCCGGCGGTCAGGTG‑ATGTTCTGAACAACAG‑CGGAGTTCCATAAAGGATCTCGGTGAGACGATAGAATGCCTGATGCGCTACGCTCATAATGCATAGAGGTGTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACtc < 2:278544/244‑1 (MQ=255) aCAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATAAAGGATCTCCGTGAGACTAAAGAAGGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGGTATGGTATCCGCACtca < 1:188646/251‑1 (MQ=255) aaaGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCCCTACATTAAGGAAAAGTTATGGTATCCGCACtcatc > 1:240173/1‑251 (MQ=255) aaaGTACAGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACtcatc > 1:197083/1‑251 (MQ=255) cccGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTTTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGGCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGt < 1:515377/250‑1 (MQ=255) ccGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCAAAGGCGTCGTACGCCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATAAAGGATCTCCGTGAGACTAGAGAATGCCGGATGCGCTCCGCTCAGCGGGCATACGGGCCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTc < 2:269253/251‑1 (MQ=255) ccaaaccgcgGCTGCGATTATCAAGTTGTGCTGAATAGGCTGCTGTTCTACGCCAGTCCTAAGGCGGCGTCCTGCCCCGGACAGTCGATCAATGTCACCGGCGGTCGGGTG‑ATGTTCTAATCAACAG‑CGGAGATCCATAAAGGATCTCCGGGAGACTATAGAACGCCGGATGCGCTACGCTCACCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGcc < 2:197083/245‑1 (MQ=255) aaaaCGCGCCTGCGATTATCAAGATGTGCTGAATACGCTGCTTTTCTCCGCCCGCCCTAGGGCGGCTTACTGCACCGGACAGTCGATAAATGTCCCGGGGGGTCAGGTG‑ATGTTCTGACCAACGG‑CGGAGACCCATAAATGATCCCCGTGAGACTATAGAATCCCTGATGCGCTACCCTCATCGGGCATACAGGCCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGt < 2:96599/250‑1 (MQ=255) aaaCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAg < 1:413126/124‑1 (MQ=255) aaaCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGG‑GTGTTCTGATCAACAg > 2:413126/1‑124 (MQ=255) aCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCCCTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAt > 1:181402/1‑251 (MQ=255) aaatgggCTGAATATGCTGTTTTTCTCCGCCAGGCGGAGGGCGTCGTACTGCACAGGACAGTCGATCAATGTCACGGGCGGTCAGGGG‑ATGTTCTGATCAACAG‑GGGAGATCAAGTAAGGATCGCGGGGAGACTAAAGAATGCCTGAGGCGCTACGCACATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACt < 2:109517/245‑1 (MQ=255) aGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑GTGTTCTGATCAACAG‑CGGCGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTCCGCCCCTCAGGCATACCAGACTTCCGCCACTACATTAAGGAAAAATTATGTGATCCGCACTCCTCACCACCGCCCTTCT‑CCCCTGGTGTGCGCAACt > 2:562551/1‑251 (MQ=255) gttcttaaTATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCCCCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGATTGCTTGATGCGCTACGCTCATAGGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGcc < 1:47814/245‑1 (MQ=255) gctgatattgctgcTTTTCTACGCCAGTCTAAAGGCGCCTTGCGGCACCGGACAGTCGATAAATG‑CCCCGGGGGGGAGGTGGATTTTCTGACCAACGG‑CGGAAATCCATAAGGGATCCCCGGGAGACTATAGAAGGCCTGATGCCCGACGCTCACAAGGCATAGAGGCTTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCtt < 2:553890/243‑1 (MQ=255) atGCTGCTGTTCAACGCCAGTCCAAAGGCGTCGTACTGCACCGGTCAGGCTATCAATGGCACCGGCGTTCAGGTG‑ATGTTCTGAACAACAG‑CGGATATCCATTAAGGAGCTCCGTGAGACTATAGAATGCCGGAGGCGCTCCGCTCACCAGGCATACAGGTCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAggcggc < 1:504084/251‑1 (MQ=255) gctgTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCCACAG‑CGGCGGTCCATTAAGGATCTCCGTGAGACTATAGCATGCCTGATGCGCTACGCTCATCAAGCATACAGGACTTCCGCCCCTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGc > 1:304228/1‑250 (MQ=255) gctgTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAAAGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGcc > 1:188630/1‑250 (MQ=255) ctgTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCCGGTG‑ATGTTCTGATCAACAG‑CAGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCCTCCGGCATACCGGACTTCCGCCCCTACATTTAGGAAAAGTTATGGGATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGGGTGCGCAACTGGCCTTAGGCGGCTGGCa > 1:374847/1‑250 (MQ=255) cGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGGCAGGGG‑CTGTTCTGGTCAACAG‑CGGAGATACATTAAGGATATCCGTGGGACTATAGAAAGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTTCATTAAGGGAAAGGTATGGTCTCCGCCCTCATTACCGTCGCCGTTCTACGCC‑GGGGGGCGCCACTGGCCTTAGGCGGATGGCaaacttttcg > 2:406992/1‑244 (MQ=255) cagcccTAGGGCGTCGTACTGCCCCGGACAGTCGATCAATGTCACCGGCGGTCGGGTG‑ATGTTCTGACCAACAG‑CGAAGATCCATAAAGGATCCCCGTGAGACTAAAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGttt < 2:181402/247‑1 (MQ=255) aagggtcggacccgCACCGGAAAGGCGTTCAATGTCCCGGGCGGTCAGGGT‑ATGTTCTGACCAACAG‑GTGAGACCCATTAAGGATCTCCGAGAGAATATAGAATGCCTGATTCTCTACGCTCATAAGGCATATAGGCCTGCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTGTAACCGt < 2:214957/238‑1 (MQ=255) gCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGGTCTCCGTGGGACTATAGAAAGCCAGATGCGCTACGCTCCTCAGGCATACAGGACTTCCGCCACTACCTTAAGGAAAAGCTATGGTATCCGCACTCATCACCGTCGCCGGTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCCAATTTCTCGTTTTaaacgcgcc > 1:332025/1‑244 (MQ=255) gtcgtGCTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑GGGATATCCATAAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCtt < 1:15011/251‑1 (MQ=255) tcgtATTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑AAGTTCTGATCAACAG‑CGGGGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGGATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCCAATTTTTCGTTTTAACCGTGCCttt > 1:473032/1‑250 (MQ=255) cggacatgcGATCAATGTCCCCGGCGGTCGGGTG‑ATGTTCTGAACAACAG‑CGGAGACCCATTAAGGATCTCCGTGAGACAAGAAAATGCCGGATGCGCTACCCCCACCAGGCATACGGGACTGCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCGCGTTTTAACCGTGCCTGCGACACACTATg < 2:304228/243‑1 (MQ=255) aGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATGAAAAG‑CGGAGATCCATTAAGGATCTCCGGGTGACTATAGAATGCCTGATGCGCTAAGCCCATCAGGCATACAGGGCTTCCGCCCCTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGGGGGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGc > 1:366450/1‑251 (MQ=255) gATCAATGTCACCGGCGGTCAGGGG‑ATGTTCTGATCAACAG‑CGGAGATCCACTAAGGGTCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTTCGCCACTACATTAAGGAAAAGGTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCCAATTTCTCGTTTTAACCGTGCCTTCGACACACTTTGCCAGCAAgg > 2:115204/1‑251 (MQ=255) ggtccccGGCGGTCGGGTG‑ATGTTCTGATCAACAG‑GGGAGATCCATTAAGGATTTCCGGAAGACTATAGAATGCCTGACGCGCTGCGCTCACCAGGCATGGAGGACTTCCGCCACTACATTAAGGAAAAGTTATGTTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGGAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGGt < 1:56266/246‑1 (MQ=255) cccGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGGGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACGGGGCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑c < 1:218435/160‑1 (MQ=255) ggcggTCAGGTG‑ATGTCCTGATAAACAG‑CGGATATCCATTAAGGATCTCCTGGAGAATAAAGAATGCCTGAGGCGCTCCGCTCAGCGGGAATACAGGCCTCCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGGTTGGCGTg < 2:136494/251‑1 (MQ=255) gTCAGGTG‑ATGTTCTGTTCAACGG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTAATGCGCTCCGCTCATCAGGCATACAGGCCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTGCGACACACTATGCCAGCAAGGGCGGGTTGGCGTGGGcc < 1:538687/251‑1 (MQ=255) tCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCCAGGGCGGGTTGGCGTGGGCCg > 1:260546/1‑251 (MQ=255) | AGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTG‑ATGTTCTGATCAACAG‑CGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAT‑CGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGGTTGGCGTGGGCCG > NC_000913/2828257‑2828751 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |