| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,458,698 | 0 | C | A | 24.6% | 118.1 / 12.5 | 69 | I120I (ATC→ATA) | gspE | Type II secretion system protein GspE |
| Reads supporting (aligned to +/- strand): ref base C (36/16); new base A (1/16); total (37/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.49e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATG > NC_000913/3458454‑3458948 | atatTCCACGTAAGGTTGGCCAGAGCCAGACCATGAACAGCTTGTCACCGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGGGTCGCAACAGATTGCGCAGGATAT‑CGACCAATCCGTCGTTCTTCTCTCGCTTTCGGCAGAGATGACGGCAAATGAAGAACTCCTGAATGAAGTTTCAGCGGCACC‑GGTTATCCGATTG‑ATCAATGCGATTTT‑AAGTGAGGCCATCAAAGa > 2:506430/1‑250 (MQ=255) tatTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGCTTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGCTTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGCTTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GCGTGGGGCCATCAACGaa > 2:399733/1‑250 (MQ=255) atTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAAc > 2:460913/1‑251 (MQ=255) cAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCg < 1:204941/250‑1 (MQ=255) tCAGGTTGGCCAGTGCCAGAACCTGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGCTTGCGCGGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGCTTTT‑GAGTGAGGCAATCAAAGAAACCGCCTCTg > 2:513839/1‑251 (MQ=255) gggtgggCCAGTGCCAGACCATGCCCAGTTTGTCAAAGGTTGATTTTGAAGTGCGGCGGGAAGCGGTATTCCATCAGAATACGGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCTTGAAGGAAGATTCAGCGGCACC‑GGTTACCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCAGCAAAGAAACCGCCTCTGat < 2:296237/246‑1 (MQ=255) aGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCGGGAAGCGGTATTCCATCAGAATCCCGGTGATTCGCAACAGATGGCGCAGGATAT‑CGATCAATCCGTCGATCTTCCCTCGTTTTCGGAAGAGATGCCCGAAAATGAAGATCTCCTGAAGGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGTTTTG‑GAGTGAGGCCATCAAAGAACCCGCCTCTGATATCCACAtt < 1:300315/251‑1 (MQ=255) gTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGaggttgacgtaattggtggatcc > 2:202401/1‑228 (MQ=255) gTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGATCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTg > 1:292307/1‑251 (MQ=255) tccaaaCCATGACCAGGTGTTCACGGGCTAATTTTGAAGCCCGTCGGGAGTCGGTATTCCATCAGAATACCGGTGCTTCCCAAAAGATTGCGCAG‑ATTTGCGATCAACCCGTGGTTCTTCTCTCGCTGCCGGAAGAGATCCCCGCAAATAAAGATCTCCTGAATGAGTATTCCGCGTCACG‑GTTTATCCCTTTA‑ATCATGGCGATTTG‑GAGTGGGGCCATAAAAGAAACCGCCTCTGATATCCACATTGa < 2:95781/245‑1 (MQ=255) cAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATCCCGGTGAGTCGCAACAGATTGCGCAGGATAG‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAAGGAATATCTCCTGAATGAAGATTCAGCGGCCCG‑GGTTATCCTCTTG‑ATCAATGCGATTTT‑GAGGGTGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAAc < 1:579290/251‑1 (MQ=255) aGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAAcc > 2:216442/1‑251 (MQ=255) gACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCctc < 1:156653/231‑1 (MQ=255) gACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCctc > 2:156653/1‑231 (MQ=255) cAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGaaaaa > 2:68567/1‑250 (MQ=255) gCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCCGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGCAGAGATGCCCGCAAATGAAGATCTCCTGACTGCAGATTCTGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAGGAAACCGCCTCTGTTATCACCATTGAAACCTATGaaacaaa > 2:157875/1‑246 (MQ=255) caGGCTAATTTGTAAGCTCGGTTGAAAGCGTTATTCCATCAGAAGCCCGGTGATTCGCAACAGATGGCGCGGGATTT‑CGATCAAGCCTGCGATCTTCTCTCGCTTTCGGAAGCGGTGCCCACAAATGAAGAGCTCCGAAATGAAGATTCAGCGGCCCC‑GGTTAGGCGCTTG‑TGCAATGCGATTTA‑AAGTAAGCCTACCAAAAAAACCTCCTCTTAGATCCACATTGAAACCTATGAAAAAACAATGTCGAt < 1:393045/251‑1 (MQ=255) taTTTTGAAGCTCGGCGGGAAGCGGTATTCCATCAGAATACCGGGGAGTCGCAACATAGTGCGAAGGATAT‑CGATAAACCCGTCGATCTTCTCTCGCTTTCGGAAGAGTTGCCCGAAAATGAAGATCTCGTAAATGAGGATTCAGGGCCTCC‑GGTTAGCCGCTTT‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAATCCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGtt < 1:584824/249‑1 (MQ=255) gATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGCGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGttt > 1:55104/1‑251 (MQ=255) gATTTTGAAGCTCGGCGGGAAGCGGTATTCCATCAGAATACCGGGGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAAGGCGATTTT‑GAGGGAGGCCATCAAAGAATCCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGt < 1:366994/249‑1 (MQ=255) ttggaagtccGGCTGGAAGCGGTATTCCACAAAAATACCGGTGAGTCGAAACAGATGGCGAAGGATAT‑CGATCAATCCGTCGATCTCCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATAAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTc < 2:136388/241‑1 (MQ=255) aaGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATc > 1:530294/1‑251 (MQ=255) gAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGtttt > 1:18867/1‑251 (MQ=255) aaGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTg > 1:191475/1‑251 (MQ=255) gttATTCCATCAGAATACCGGTGAGCCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATAAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCg < 1:189533/247‑1 (MQ=255) ggTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCg > 2:189533/1‑249 (MQ=255) gTATTCCATCAGCATACCGGTGAGTCGCAACAGATTGCGCAGGTTAT‑CGATCAATCCGTCGATCTTCTCTCACTTTCGGAAGAGGTGCACGCACATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGATATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAACCCGCCTCTGAGATCCACATTGAAACCTATGAACAAACAAGGTCGATCCGTTTTCGCATctcccgctttttgcggcc > 2:4804/1‑234 (MQ=255) aTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGcc < 1:304453/178‑1 (MQ=255) aTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGcc > 2:304453/1‑178 (MQ=255) tccagcaaaaTACCGGTGAGTCCCAACAGATTGGGCGGGATAT‑CACACACTCCGTGGTTCTTCTCCCGCTTTCGGAAGAGCGGCCCGCAAAGTAAATTCTCCTGAATGAAGATTCCGGGGCCCG‑GGTTACCCGCTTG‑ATCATTCCGATTTT‑AAGGGAGGCAATAAAAAAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAAt < 2:550936/242‑1 (MQ=255) ccatcagacaacggttgagtccaaaCAGATTGCGCAGAATAT‑GGATCAATCCGTCTAACTTCTCTCGCTTTCGGAAGAGATCCCCGCAAATAAAGATCTCTTGAATAAAGATTCAGCGGCACT‑TGTTATCCTCTTG‑ACCAATGCAATGTT‑GGTTGAGCCCATAAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCTTTTTCGCATCGACGGCGTTTTGCGGACAAttt < 2:217663/228‑1 (MQ=255) ccgtgtgactagCAACAGATGGCGAAGGATAT‑CCATCAACCCTTCGATCTTCCCCCCCTTTGGGAAGAAATGCCCGCAAATAAAAATCTCCTAAAGGAAGATGCGGCGGCACG‑GGTTACCCGCTTG‑ATCAAGGCGATTTT‑GAGGGAGCCCACAAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCcaaa < 2:36423/240‑1 (MQ=255) aCCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCcaa > 1:413792/1‑250 (MQ=255) cGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCg > 1:279530/1‑194 (MQ=255) gCAACAGATTGTGCCGGATCT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCAATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGAAAATTTTACAGCCAAACACAAAACTgg > 2:75923/1‑251 (MQ=255) gCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GCGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATAGAAACCTATGAAACAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGCCAATTTTACAGCCAAACAAAAAACTgg > 1:21907/1‑251 (MQ=255) aaCAGATTGCGCAGGATAT‑CGATAAACCCGTCGATCTTCTCTCGTTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAGTGAAGATCCGGCG‑CACCCGTTTACCCGCTTG‑ATAAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGGCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTggcg < 1:412493/251‑1 (MQ=255) gcgcAGGATTG‑CGATCAATCCGTCGATCTTCTCTCGCTGTCGGAAGAGATGCCCGAAAATGAAGATCTCCTGAATGAAGATACAGCGGCACC‑GGTTATCCGCTTG‑ATAAAGGCGATTTT‑GAGTGAGACCATAAAAAAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCt < 2:18867/251‑1 (MQ=255) atat‑CGATCAATCCGTCGATCATCTCTCGCTTTCGGAAGAGATGCCCGCAAATGGAGATCTACTGACTGCAGATTCCGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTcc > 2:181047/1‑251 (MQ=255) at‑CGATCAACCCGTCGATTTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATAAAAAAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTccc < 1:145645/250‑1 (MQ=255) aTCAACCCGTCGATCTTCTCTCGCTTTCGGAAGAGAGGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCCCC‑GGTTACCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAAtt < 1:460913/251‑1 (MQ=255) tcactCCGTCGAGCTTGTCTCGCTTTCGGTTGAGCTGCCCGCAAATGATGCTCTAATGAATGAAGATTCATCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTG‑GAGTGAGACCATCAAAGAAACCGAATATGATATCCACATTGAAACCAATGAAAAAACAATGTCGATCCGTTTTCGAATCGACGGCGTTTTGCGGACAATTTTACAGCCGAACAATAAACTGGGGGCACTGCTTACCTCCGGAAtt > 2:116463/5‑250 (MQ=255) tCAATCAGACGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGACGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCCAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAAtt > 2:217119/1‑250 (MQ=255) acccgtctatttcctctcGCGTTGGGAAGAGAGCCCCCCAAAGTAAGATCTCCTGAATAAAGATTCAGCGGCCCC‑GGTTATC‑GGTTGTATCAATG‑GATTTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAgg < 2:419900/238‑1 (MQ=255) ccgcggttcttcccccgtttgcGGAAGAGATGCCCGAAAATGAAAATCTCATGAAGGAAGATACAGGGGCACC‑GGTTTCCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCACAAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTCCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTc < 2:327429/230‑1 (MQ=255) gTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATCAAGATATCCTGAATGAAGATTCAGCGGCACC‑GGTTTTCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATAAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAACAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATATTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCAt > 1:522844/1‑251 (MQ=255) cGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGTCACTGCTTATCTCCCGATTTAAGGTCATgg > 2:19617/1‑251 (MQ=255) gATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCCTGGc > 2:314216/1‑251 (MQ=255) gATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGCCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAAAGTCGATCCGTTTTCGAATCGACGGCGTTTTGCGGACAATTTTACAGCCAAAAAAAAAACTGCCGGCACTGCTTATCTCCCGAATTAAGGTCATGGc > 1:375654/1‑251 (MQ=255) ttctCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGt > 1:43076/1‑251 (MQ=255) cctcGCTTGCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAAGGAAGATTCAGCGGCCCC‑GGTTATCCGCTTG‑ATAAATGCGATTTT‑GAGGGAGGCCATAAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCtt < 1:68567/250‑1 (MQ=255) gCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGat > 1:423046/1‑250 (MQ=255) tttCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGatatt > 2:560408/1‑250 (MQ=255) ttCGGAAGAGATGCCCGAAAATGAAGATCTCCTGAATGAAGATTCAGCGGCCCC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATAAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGatat < 1:159545/249‑1 (MQ=255) gcGGAAGAGATGCCCGAAAAGGAAGATCCCCTAAATGAAGATTCGGCGGCACC‑GGTTACCCGCTTG‑ATAAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCg < 1:489700/249‑1 (MQ=255) aaTGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCCCC‑GTTTATCCGTTTG‑ATCAATGCGATTTG‑GAGTGAGGCAATAAAAAAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAAc < 1:560408/250‑1 (MQ=255) aTGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGGCTTGATATCGGCGGAAAAcc > 2:148804/1‑250 (MQ=255) aTGCCAGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAACGAAACCGCCTCTGATATCCACATTGACACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTACGGTCATGGCTCGTTTTgcgatttccgtaaaacc > 2:520061/1‑235 (MQ=255) aaaGATCTCCTGAATGAAGATTCAGCGGCCCC‑GGTTATCCGTTTG‑ACAAATGCGATTTT‑GAGTGAGCCCACAAAAAAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGa < 1:216442/250‑1 (MQ=255) aatcccataaatgaaGTTTCGGGGGCACC‑GTTTATCCGCTTG‑ATCATTGCGATTTG‑GAGTGAGGCCACAAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCGTGATATCGCCGAAAAACGTATTCCACAGGATgg < 2:43076/242‑1 (MQ=255) atgaaGATTCAGCGGCACC‑GGTTATCAGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGATCGGCTGGATAACCCCGGAAAACGAATTCCACCGGGTGGGAGAAtaatt > 2:409695/1‑247 (MQ=255) cGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGCAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCggg > 1:21562/1‑251 (MQ=255) ttATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACCTATTCCACAGGATGGAAGAATTAGTTTGCGGATCGGGCGACGTaa > 2:509802/1‑250 (MQ=255) aTCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACt > 1:370808/1‑143 (MQ=255) ccgctgaaTCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAg < 1:19617/244‑1 (MQ=255) g‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTcc < 1:212136/251‑1 (MQ=255) g‑ATCAATGCGATTTT‑GAGTGAGGCCATAAAAAAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACGGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGTGCGACGTAACATAGATGTcc < 1:217119/251‑1 (MQ=255) aaTGCGATTTT‑GAGTGAGGCCATCAAAAAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGt < 1:174732/251‑1 (MQ=255) aTGCGATTTT‑GAGTGAGGCCATAAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTa < 1:166499/251‑1 (MQ=255) cAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGGCTTGGTCTCGCCGCAAAACGCATTCCACAGGATGGGAGAATTAGTTTGCGTATCGGGCGACGTACAAGAAATGTCCCCGTTTCCACCCCGCCGTCCATCtctg > 2:437428/1‑248 (MQ=255) | ATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATAT‑CGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC‑GGTTATCCGCTTG‑ATCAATGCGATTTT‑GAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATG > NC_000913/3458454‑3458948 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |