| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,014,222 | 0 | C | A | 27.4% | 90.9 / 18.5 | 62 | Q332K (CAA→AAA) | fliF | flagellar basal‑body MS‑ring and collar protein |
| Reads supporting (aligned to +/- strand): ref base C (40/5); new base A (0/17); total (40/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.56e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGG > NC_000913/2013973‑2014466 | aTCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTCCCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTACGGCAAATc > 1:338643/1‑250 (MQ=255) cTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCACATCaaa > 2:128687/1‑250 (MQ=255) tCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCCGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGAGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATa > 1:579596/1‑250 (MQ=255) ccTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGGTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGACTCAACC‑GGCCCCTGCGAATAAC‑GCGCCAATCCGCACGCCTCCGGCAAATCAAAATAACCg > 2:227529/1‑251 (MQ=255) gTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGc > 1:258752/1‑251 (MQ=255) atatTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGGGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCCGCAGCAGACGAGcacc > 2:370996/1‑251 (MQ=255) cccaaGGTTCCGGCGCAGCTGAACTTCCCCAGAAAAAAACAAACGGAAGAACAGTATCGCCCTACGGGTGATGAATCTCAGGCGGCGTTTCGTTCACGCCAGTTAAATTGTG‑GCGGGCAAAGCGGTCCGGGTTA‑CCCGTGCGGCTTCCCGGGGGCTTTGC‑CGAA‑CAACCCGGCACCTGCGAAAAAC‑TCCCCAATCAGCCCGCCCCCGGCAAACAAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCaa < 1:544443/247‑1 (MQ=255) cAGGTTACGGCGCAGTTGGACTTCGCCAGTAAAGAAAAAACGGAAGAACAGTATCGCCCAAACGGTGATGAATCTCATGCGGCGCTTGGTCCACGCCAGCTTAAT‑GAGAGCGAGCAAAGGGGTTCGGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGC‑AGAATCAACC‑GGCCCCTGCAAAAAAC‑GCGCAAACCAGCACGCCTCCGGCAAATCAAAAAAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAg < 1:466206/251‑1 (MQ=255) aGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTACCGGTGATGAATCTCATGCGGCGCTTCGTTCACGCAAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACATGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCCATAGt > 1:30854/1‑251 (MQ=255) aGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑GGAATCACCC‑GGCACCTGCGAGTAAC‑GCGCCACTCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGCGCACCACCAGCAATAGt > 2:61669/1‑251 (MQ=255) aGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCCCCCGCAATAgg > 2:93698/1‑250 (MQ=255) aGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGt > 1:123185/1‑251 (MQ=255) aCGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGcc > 2:376021/1‑251 (MQ=255) ggCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCcgc > 1:333574/1‑251 (MQ=255) gcAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGt > 2:107026/1‑250 (MQ=255) gCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCCCCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGa > 2:544430/1‑250 (MQ=255) cagaaaaGAACAAACGAAAGAACATTATCGCCCAAACGGTGTTAAATCTCATGCGGCGTTCCGTTCCCGCCAGCTTAAT‑GAGAGGGGGCAAAGCGGTTCCGGTTA‑CCGGGGCGGCGTCCGGGGGGCGTTGT‑CGAATCACCC‑GGCACTTGCGAATAAC‑GCGCCAACCAGCACGCCTCCGGCAAATAAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATg < 1:533467/247‑1 (MQ=255) cAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATg > 1:476310/1‑251 (MQ=255) aaaaCGGAAGAACAGTACCCCCCTAACGGTGATAAATCTCGTGCGGCGCTTCGTTCCCCCCAGCTAAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑CCCGGGGGGCGTCCGGGGGGCGTTGT‑CGAACCACCC‑GGCCCCTGCGAATACC‑GCGCCAATCAGCACGCCTCCGGAAAACAAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTaa < 1:503516/250‑1 (MQ=255) agaaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGa > 1:221725/1‑249 (MQ=255) cAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCCGCTTAAT‑GAGAGCGAGCCAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGACTGAAACCAGTAACTACGAAGTCgc > 1:504584/1‑250 (MQ=255) tATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCg > 2:250660/1‑251 (MQ=255) tCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCCGTAACTACGAAGGCGATCGcc > 2:462274/1‑250 (MQ=255) tAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGAGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTc > 1:310067/1‑251 (MQ=255) accGGTGATGAATCCCATGCGGCGCTTCGTTCCCGCCAGCTTAAT‑GAGAGCGAGCAAGGCGGTTCCGGTAA‑CCGGGGCGGCGTACGGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCAAATACC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAAAAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCAtt < 1:494992/247‑1 (MQ=255) aacgggtatgaATCTCAGGCGGCCCTTCTTTCCCGCCAGTTTAAT‑GAGAGCGAGAAAAGCGGTTCCG‑TTACTCCGGGCGGCGACCCGGGGGGGTTGC‑CGAACCAACC‑GGCCCCTGCGAATACC‑GCCCAAATCAGCACGCCCCCGGAAAATAAAAATACCCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTc < 2:393939/243‑1 (MQ=255) aaCGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑CCCGGGGGGCGTACCGGGGGCGTTGC‑CGAATCAACC‑GCCCCCTGCGAATAAC‑GCGCAAATCAGCACGCCCCCGGCAAATAAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCg < 1:376021/251‑1 (MQ=255) tgaATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTCCCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCACTCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATAcc > 2:240320/1‑250 (MQ=255) cggcggggCTCCGTTCACGCCACCTTAAT‑GAGAGGGAGCAAAGCGTTTCCGGTTA‑CCCGGGGGTCGTACCGGGGGCTTTGT‑CGAATCAACC‑GGCCCCTGCGAATAAC‑GCGCAAATCAGCACGCCTCCGGCAAAAAAAAAAAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAAc < 1:28512/249‑1 (MQ=255) tCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGc > 1:117961/1‑250 (MQ=255) cACGCCAGATTAAG‑GAGGGCGAGCAAAGCGGTCCCGGTTA‑CCCGGGGGGCTTCCCGGGGGCGTTGT‑CGAACCAACC‑GGCACCTGCAAATAAC‑GCGCAAATCAGCACGCCTCCGGCAAATAAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCaa < 1:227529/251‑1 (MQ=255) tAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTggc > 1:340809/1‑247 (MQ=255) gcgagcAAAGCGGTTCCGGTAA‑CCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCACCC‑GGCACCTGCGAAAAAC‑CCCCCAATCAGCACCCCCCCGGCAAACAAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTc < 1:544430/251‑1 (MQ=255) gcgagcAAAGCGGGCCCGGTTA‑TCCGGGCGGCGCACCGGGGGCGTTGT‑CCAATCAACC‑GGCACCTGCGAATAAA‑GCGCCAATCAGCACGCCTCCGGCACCTCAAAATACCCGCCAGCAGCAGGCGAGCACCACCAGAAATCGTGGGCCGCGTAGCACACAGCGGAATGAAAACAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGAGGTTGACCGTCCGTCAGTCGCggcc > 2:263864/1‑249 (MQ=255) ccgagtaaaccgggcccggtaacccGGGCGGCGTCCGGGGGGCGTGGT‑CGAACCAGCC‑GGCCCCTGCAAATAAC‑GCGCCAATCAGCCCGCCTCCGACAAACAAAAAGAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATTAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTc < 2:30854/228‑1 (MQ=255) gcAAAGCGGTTCCGTTTA‑CCCGGGCGGCGTACGGGGGGCGTTGT‑CGAACCAACC‑GGCCCCTGCGAATACC‑GCGCCAATCAGCACCCCCCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCgtgg < 1:250660/251‑1 (MQ=255) cGGTGCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGGGAATAAC‑GCGACAAGCAGCACGCCTCCGGCACATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGGGGGCCGCGGAGCACACAGCGGAATGAGACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGAGGGCGATGTGCAACGTCTGTCAGTCGCGGGCgtgggacatt > 2:80139/1‑245 (MQ=255) tCCGGTTA‑CCCGGGCGGCGTCCCGGGGGCGTTGT‑CGAACCACCC‑GGCCCCGGCGAAAAAC‑GCCCCAATCACCACGCCCCCGGCAAATAAAAAAAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACa < 1:93698/250‑1 (MQ=255) ggTTA‑TCCGGGGGGCGTGCGGGGGGCGTTGT‑CAAATCAACC‑GGCACCTGCAAATAAC‑CGGCAAACCACCCCGCCCCCGGCAAATAAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGGCAGACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAAc < 1:552577/251‑1 (MQ=255) ttA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGACTTACAAAACCt > 2:574830/1‑251 (MQ=255) a‑TCCGGGCGGGGTACGGGGGGCGTTGG‑CAAATCACCC‑GCCCCCGGCGA‑TAACCGCCCCAATCAGCCCCCCTCCGGCAAAAAAAAATATCCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTg < 1:462274/251‑1 (MQ=255) a‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAA‑GCGCCAATCAGCACGCCTCCGGCCAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTg > 1:62485/1‑251 (MQ=255) ccGGGGGGCG‑ACCGGGGGGGGTGTACGAAACAACC‑GGCACCTGCAAAAAAC‑CCCCCAACCAGCACGCCCCCGGAAAATAAAAAAAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGcc < 1:370996/251‑1 (MQ=255) gggCGGCGTCCCGGGGGCGTTGC‑CGAATCAACC‑GCCCCCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAg < 1:128687/251‑1 (MQ=255) ggcggcGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAACTCAAAATACCCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCAt > 1:235539/1‑173 (MQ=255) ggcggcGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGc > 1:377949/1‑198 (MQ=255) gcggcgTACGGGGGGCGTTGT‑CGAATCAACC‑GGCCCCTGCGAAAAAC‑GCGCCAATCAGCGCGCCTCCGCAAAATAAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGAt < 1:574830/251‑1 (MQ=255) ggggCGTTGT‑CGAATCACCC‑GGCCCCTGCGAATACC‑GCGCCAACCAGCACGCCTCCGGCAAATAAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGtt < 1:345480/251‑1 (MQ=255) gTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGAACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGAGTAACACACAGCGGAATGAACCCAGTAACTACGAAGTCGATCGAACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGAGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCctc > 1:535331/1‑251 (MQ=255) ttGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCCCACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCctct > 2:85537/1‑251 (MQ=255) ggaaccaacggcccccTGCGAATACC‑CCGCCAAGCAGCTCGCCTCGGGCAAGTCAAAATAGCCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGGGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAg < 1:251090/236‑1 (MQ=255) aaCC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGa > 1:278947/1‑250 (MQ=255) c‑GGCACCTGCGAATAAC‑GCGCCAATCAGCAAGCCTCCGGCACATCAAACTAACCGCCAGCGGCACGCGATCACCACCAGGGATAGTGGGCCCCGGAACACACACCGGCATGAAACCAGTAACTACGAAAACGATCGCACCATTCGCCATACCAGAATGAACGTGTGCGATGTGCCACGTCTGTCAGTCGCGGACGTGGGCGATTACAAAACCTTGCCAGATGGCACACCGTGGCCTCTCAGCAACGGACac > 1:339659/1‑250 (MQ=255) ggCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAAc > 1:251772/1‑248 (MQ=255) cACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAAACGCAAGCAGCAGGCGCGCCCCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATCCCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGACTCTCAGCAACGAACAGATg > 1:188700/1‑251 (MQ=255) cccaaccccagcccccccccGGCAAATAAAAAAAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGaa < 2:449966/231‑1 (MQ=255) aTCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGAc > 1:217312/1‑251 (MQ=255) cAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGaa > 1:449966/1‑241 (MQ=255) ccTCCGACAAAAAAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGc < 1:240320/251‑1 (MQ=255) cTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCCCCCCCAGCAATAGTGGGCCGCGTAGAACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCg > 2:280923/1‑251 (MQ=255) cTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGCACGTGGGCGATGTGCACCGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCg > 1:571201/1‑251 (MQ=255) tCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCg > 1:250087/1‑250 (MQ=255) gCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATggg > 2:46801/1‑251 (MQ=255) | ATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAT‑GAGAGCGAGCAAAGCGGTTCCGGTTA‑TCCGGGCGGCGTACCGGGGGCGTTGT‑CGAATCAACC‑GGCACCTGCGAATAAC‑GCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGGG > NC_000913/2013973‑2014466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |