breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 23 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913786,8550AC38.1% 111.3 / 11.2 65G247G (GGT→GGGgpmA2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase
*NC_0009132,228,2780GC31.2% 75.2 / 12.0 49intergenic (‑1008/‑704)yohF/yohPputative oxidoreductase YohF/putative membrane protein YohP
*NC_0009131,557,3240TG31.1% 109.9 / 14.9 61T239P (ACC→CCC) ddpFputative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpF
*NC_0009132,434,8820TG30.8% 139.1 / 16.7 81Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
*NC_000913811,7210TG29.2% 181.1 / 11.7 109V67G (GTG→GGG) bioCmalonyl‑acyl carrier protein methyltransferase
*NC_0009134,296,2680TC28.1% 33.0 / 20.2 34intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009134,256,0610CT28.0% 73.7 / 13.8 50E136K (GAA→AAA) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase
*NC_000913766,7160GT27.8% 49.8 / 10.2 36A245S (GCG→TCG) mngA2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
*NC_0009134,457,1050AG27.3% 190.8 / 13.6 91I441V (ATC→GTC) mplUDP‑N‑acetylmuramate‑‑L‑alanyl‑gamma‑D‑glutamyl‑ meso‑2,6‑diaminoheptanedioate ligase
*NC_0009131,014,0280AG26.9% 145.7 / 14.8 67D257G (GAT→GGT) pqiBintermembrane transport protein PqiB
*NC_0009132,434,8610GT26.7% 123.9 / 13.3 75P259Q (CCA→CAA) truAtRNA pseudouridine(38‑40) synthase
*NC_0009131,265,4820TG26.4% 162.9 / 14.7 88G157G (GGT→GGGprfApeptide chain release factor RF1
*NC_0009134,465,7870GA26.3% 59.5 / 13.2 38R132C (CGC→TGC) treBtrehalose‑specific PTS enzyme IIBC component
*NC_0009134,256,0690GC26.0% 93.2 / 13.4 50P133R (CCG→CGG) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase
*NC_0009131,825,0610GA25.6% 88.7 / 11.3 43intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
*NC_0009132,244,2120CT25.4% 100.6 / 10.9 67G101G (GGC→GGTyeiGS‑formylglutathione hydrolase/S‑lactoylglutathione hydrolase
*NC_0009133,556,2070TC23.7% 145.4 / 10.6 60S215G (AGT→GGT) rtcARNA 3'‑terminal phosphate cyclase
*NC_0009131,130,3740CT22.9% 175.0 / 14.5 83A164T (GCG→ACG) flgAflagellar basal body P‑ring formation protein FlgA
*NC_0009132,434,8630AG22.9% 145.2 / 11.8 74L258L (CTT→CTCtruAtRNA pseudouridine(38‑40) synthase
*NC_0009132,557,4690TG22.1% 195.5 / 10.6 86Y404S (TAC→TCC) eutBethanolamine ammonia‑lyase subunit alpha

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 69674143 (0.560)5 (0.090) 5/338 3.9 12.3% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 42 (0.800)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 91886167 (0.870)3 (0.040) 3/478 5.9 3.9% coding (260/993 nt) ybjX DUF535 domain‑containing protein YbjX
?NC_000913 919612 = 84 (1.120)coding (378/1116 nt) macA ABC‑type tripartite efflux pump membrane fusion protein
* ? NC_000913 = 50595774 (0.960)3 (0.040) 3/494 6.0 3.9% coding (1044/1653 nt) ushA 5'‑nucleotidase/UDP‑sugar hydrolase
?NC_000913 4265850 = 72 (0.930)coding (69/1080 nt) alr alanine racemase 1
* ? NC_000913 = 175398 (1.270)3 (0.040) 3/494 6.0 3.2% coding (1417/2463 nt) thrA fused aspartate kinase/homoserine dehydrogenase 1
?NC_000913 2406303 = 82 (1.060)coding (339/939 nt) lrhA DNA‑binding transcriptional dual regulator LrhA