Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913766,7160GT27.8% 49.8 / 10.2 36A245S (GCG→TCG) mngA2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
Reads supporting (aligned to +/- strand):  ref base G (15/11);  new base T (10/0);  total (25/11)
Fisher's exact test for biased strand distribution p-value = 1.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTT‑CTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGT‑ATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAA‑CTCAAT‑AGTGC‑A‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGT  >  NC_000913/766469‑766963
                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                         
tgaGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTT‑CTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGT‑ATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAA‑CTCAAA‑AGTGC‑A‑A‑ACCCCTGGATTTTTTCgg                                                                                                                                                                                                                                                        >  1:172932/1‑251 (MQ=255)
              cgcTGGATGAAAAGGGGGGCACG‑AACCTTTCTCCCTTTTTTCCCAGCGCGCAAACTTTCGTCTGCGTAGGGGCCTGCCGGGGGGGT‑ATTGCTCCCCCCTATATGGCTGCGGAATATCGGAAAAAAG‑CCGGACAAAAACTCGGCGTAAATTTTTACTTAAAAAAACAA‑GGCGCAAACGGTATTGAGGGGTTTTTACCGGGGGATAAA‑CTAAAA‑AGTGA‑A‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATc                                                                                                                                                                                                                                          <  1:70660/251‑1 (MQ=255)
                          aGGGAGGCACGCAACCTT‑CTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGT‑ATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAACCA‑CTCAATTAG‑GG‑A‑A‑ACGCCCGTATTTTTTCGGCTGAAGTCGCCCTCA‑AGGggaggg                                                                                                                                                                                                                               >  1:352653/1‑247 (MQ=255)
                                gCACGCAACCTT‑CTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGT‑ATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAATAA‑CTCAAT‑AAGGC‑A‑A‑CCCCCCGTATTTTTTCGGCTGAAGGCGCCCTCA‑AGgagggggggcggttt                                                                                                                                                                                                                       >  1:215153/1‑239 (MQ=255)
                                  aCGCAACCTT‑CTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGT‑ATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAA‑ATCCAT‑TGTGCCA‑A‑CC‑CCTGTTTTTTTTCGGCTTGAGTTGCCATccaagggggggagcggttta                                                                                                                                                                                                                       >  1:487267/1‑231 (MQ=255)
                                      aaCCTT‑CTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGT‑ATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAA‑CTCAAT‑ATTGC‑A‑A‑ACGCCTGTATTTTTTCGGCTGAAGGCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATggg                                                                                                                                                                                                                >  1:268876/1‑250 (MQ=255)
                                          tt‑CTCCCTCTTTGCCCACCGCCCCAACTATCTTCTGCGTAGCGGC‑TGTTCGGGGGGTTATTTCTCACACCTATTGGGGTGAGAAATATC‑GGAAAAAGGCCGGCAGAAAACGCGGCAAAATTTTTTACGT‑GAAAAACAAAGGCGCTACGGGCGTGGAAGGG‑TTTTAAAGGGGGAACAAACTAAAT‑‑GTGG‑A‑AGCCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCAt                                                                                                                                                                                                              <  2:357814/249‑1 (MQ=255)
                                                             cgcgCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGT‑ATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGGTCAA‑CTCAAA‑AATGC‑A‑A‑CCGCCTGTTTTTTTTTGGCTGAAGTCGCCATCC‑AGGGGGGTGGGCGTTTT‑TATGGGGTTCCCGCGCTTTctatgcccg                                                                                                                                                                                          >  1:536136/1‑243 (MQ=255)
                                                                          gTCTGCGTAACGGCCTGTCCGGCGGGT‑ATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCCA‑CTCCAT‑AATGC‑CCA‑CC‑CCTGTATTTTTTCGGCCGAAGACGCCCTCACAGG‑GAGTGAGAGTTTTTATTGGC‑TTCCCTCGCTTTCCGTggctttttcccgaccgat                                                                                                                                                                             >  1:604104/1‑234 (MQ=255)
                                                                               cGTAACGGCCTGTCCGGCGGGT‑ATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAA‑ATCAAT‑AATGC‑A‑A‑CCGCCCGTATTTTTTCGGCTGAAGTCGCCATCA‑AGGAGAGGGAGGGGTTT‑AATGGCATTCCCGCGCTTTTAATGCCCGTTgccgggccggtttgcc                                                                                                                                                                        >  1:211131/1‑244 (MQ=255)
                                                                                                  ggT‑ATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAA‑CTCCAT‑AGTGC‑A‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGGGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGGGCCTTTTGCCGAGCCGATTCGCCATGCCGAAGCGTTGATcc                                                                                                                                                      >  1:200989/1‑250 (MQ=255)
                                                                                                      aTTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAA‑CTCAAT‑AGTGC‑A‑A‑CCGCCCGTATTTTTTCGGCTGAAATCGCCAACA‑AGGAGAGGGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCCAGCCGATTCGCCATGCAGAAGCGtttaacccacc                                                                                                                                                  >  1:247631/1‑242 (MQ=255)
                                                                                                                           gCGGAATATCTGGAAAAAG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAA‑CTCAAT‑AGTGC‑A‑A‑CCGCCTTTATTTTTGCGGCCGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATTGCATTCCCGCGCTTTCCGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTCCCCTCAAGCGt                                                                                                                               >  1:534246/1‑249 (MQ=255)
                                                                                                                            cGGAATATCTGGAAAAAG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAA‑CTCAAT‑AGTGC‑A‑A‑CCGCCTGTATTTTTGGGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGGTTT‑AATGGCCTTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACCAGCGCTTACCCTCAAGCGTAGc                                                                                                                            >  1:103607/1‑251 (MQ=255)
                                                                                                                                          aaaG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAA‑CTCAAT‑AGTGC‑A‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGcgtac                                                                                                              >  1:41193/1‑251 (MQ=255)
                                                                                                                                                     gCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAA‑ATCAAT‑AGTGC‑A‑A‑CCGCCTGTATTTTTGCGGCTGAAGACGCCATCA‑AGGAGAGGGAGCGTTTT‑AATGGTCTTCCCGCGCTTTCAGAGCCTGTTGCCGAGCCGATTTGCCATGCAGAAGCGGTTATACAAAAAGCGCATACCCTCAAGCGCAGCGATGAGACGCGGACCGTACAGCa                                                                                                     >  1:373704/1‑250 (MQ=255)
                                                                                                                                                                  aaaTTTTTATGTTAAAAAACAA‑GGCGGTAACGGGAGTAGAGGTCTTTTAACGGCGAAAAAA‑CTCAAT‑AGGAC‑A‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCgg                                                                                       <  1:411512/251‑1 (MQ=255)
                                                                                                                                                                  aaaTGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAA‑CTCAAT‑AGTGC‑A‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AAGGGAGAGAGCGTTTT‑AACGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCCCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCCCAAGCGTAGCGACGAGACGCGTACCGTAAAGCAAGATACGCCACCgg                                                                                       >  1:350055/1‑251 (MQ=255)
                                                                                                                                                                               gAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAA‑CTCAAT‑AATGC‑A‑A‑CCGCCTGTATTTTTGTGGCGGAAGACGCCATCA‑AGGAGAGTTAGCGTTTT‑AATTGCCTTTCCGCGCTTTCAATGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCACCAAGAGCTTACCCTCAAAGGGAGCGATGAGACGCGTAACGGACAGCAAGATAAGCAACCGGGGaaaaaagtaa                                                                           >  1:544117/1‑244 (MQ=255)
                                                                                                                                                                               aaaaaaCAA‑GGCGCTAACGGCTTTGAAGGGCGTTTAACGGGGGATCAA‑CTAAAA‑AGTGA‑A‑A‑CCGCCTTTATTTTTGCGGCTTAAGGCGCCATCA‑AGGAGAGTGAGCGGTTT‑AATGGCATTCCCGCGCGTTCAGGGCCTGTTGCCGGGCCGATTCGCCATGCAGAAGCGTTTATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCa                                                                           <  1:131434/249‑1 (MQ=255)
                                                                                                                                                                                      aa‑GGCGCTAAGGGCATTGAAGGGTGTTTAGCGGCGGATCAA‑CAAAAT‑AGTGC‑A‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAg                                                                   <  1:415492/251‑1 (MQ=255)
                                                                                                                                                                                              tAACGGCATTGAAGGGCGTTTAACGGCGGATAAA‑CTCCAT‑AGTGC‑A‑A‑CCGCCTGTATTTTTTCGGCTGAAGGCCCCATCA‑AGGAGAGTGAGCGTTTT‑AATTGCCTTCCCGCGCTTTCAGTGCCTTTTTCCGAGCCCGTTCGCCATGCAGAAGCGTTTATCCAACCAGCGCTTACCCTCAAGCGTAGCGATTAGACGCGTACCGAACAGCCAAATACGCCACCGGTGAAAAGTGTAAAAACCGGGCTGaaa                                                             >  1:295838/1‑250 (MQ=255)
                                                                                                                                                                                               aaCGGCATTGAAGGGCGTTTAACGGCGGATCAA‑CTCAAT‑AGTGC‑A‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATAGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAAc                                                            >  1:74071/1‑250 (MQ=255)
                                                                                                                                                                                                                    aCGGCGGATCAA‑CTCAAT‑AGTGC‑A‑A‑CCGCCTGTATTTTTGAGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAAtctc                                      >  1:144842/1‑251 (MQ=255)
                                                                                                                                                                                                                       acgggggttaaactaaagggtgaa‑a‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCGTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAAtctc                                      <  1:442628/228‑1 (MQ=255)
                                                                                                                                                                                                                             aaa‑CTCAAT‑AGTCC‑A‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGt                             <  1:176379/250‑1 (MQ=255)
                                                                                                                                                                                                                              ggcatccaacggTGC‑A‑T‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AAGGGCATTCCCGCGCTTTCAGTGCCTGTGGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGGGTCAAAGCGGAGCTGAAACAGGCACTGGTTAGCGGAATCTCTTTTGCCg                              <  2:159402/240‑1 (MQ=255)
                                                                                                                                                                                                                                  actcaagtgtcaa‑a‑CCGCCTGTATTTGTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGGTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTAc                           <  1:399134/239‑1 (MQ=255)
                                                                                                                                                                                                                                   cAAT‑AGTGC‑A‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAACAGCGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGt                        >  2:621337/1‑251 (MQ=255)
                                                                                                                                                                                                                                     aT‑AGTGC‑A‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTg                      >  1:568155/1‑251 (MQ=255)
                                                                                                                                                                                                                                     aT‑AGTGC‑A‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGAGGCTGAAACAGGCCCTGTTGAGCGGAATCTCTTTTGCCGTACCGTTg                      >  2:314791/1‑251 (MQ=255)
                                                                                                                                                                                                                                                a‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTcgc              >  1:497081/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  ccGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTcgcg             <  1:435058/251‑1 (MQ=255)
                                                                                                                                                                                                                                                           tttttGCGGCTGAAATCGACATCA‑AGGAGAGTGAGCGTCTT‑AATTGCATTCCCGCGCTTTCAGTGCCTGTTGCCGGGCCGCTTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCTTAGCGATTAGCCGCGTACCGCACAGCAAGATACGCAACAGGTGAAAACTGTCAAACCGAACCTGAACAAGGCACTGTTGAGCGGAATATCTTTTGCCGTACCGTTGATTGTCGCGGAGGGcc      >  2:118153/1‑248 (MQ=255)
                                                                                                                                                                                                                                                             tttGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACgg   <  1:170785/250‑1 (MQ=255)
                                                                                                                                                                                                                                                             tttGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGt  >  1:75876/1‑251 (MQ=255)
                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                         
TGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTT‑CTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGT‑ATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAG‑CCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAA‑GGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAA‑CTCAAT‑AGTGC‑A‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCA‑AGGAGAGTGAGCGTTTT‑AATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGT  >  NC_000913/766469‑766963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑