Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,256,0610CT28.0% 73.7 / 13.8 50E136K (GAA→AAA) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (12/24);  new base T (14/0);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.24e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAAC‑ACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGC  >  NC_000913/4255813‑4256308
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                            
atcaaccatgtctctacgaagagacAAACGGGGGCCTACGGGCGCCAGCCGATTCCGGGCAAAGTACTGCCGCGCCACGCTGGCCACTCTCTGAGCGATAGTTTGAGCCGGCCCGTATTACTCCGCCATCCGGCGCATCGAGCCTGACGGCGAGAAACGCACAAAACTGTCGCGACCTAGCCACATTGCAGCGAAAAATTTCTGTACGCCGTTAATCATTCGCAGCGGCGGG‑TTCACCTCGCCTTGTT‑CAc                                                                                                                                                                                                                                                            <  2:114041/227‑1 (MQ=255)
   aaaCAGATCGTGAGGACCGGGCAACCGGGGGCCCACGGCAGCCAGACGTGGACGGGAAAAGGTCATACGCCCCACGCGGGCCAGTTTCTGAGCGATAGTTTTATCGGTGCGGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑Gcc                                                                                                                                                                                                                                                        <  2:516731/251‑1 (MQ=255)
      cAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCg                                                                                                                                                                                                                                                     <  1:474237/251‑1 (MQ=255)
        gattcTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACCCGCGCCAGTTTCTGAGCGATATTTTTATCCGTGCCGTGTTCATCCGCCACACGGCGCAGCGAAACTGACGGCGCGACACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATCCGCAGCGGCGGC‑GTCCCTTCGCCCTTTT‑CCC‑CCCCCGgc                                                                                                                                                                                                                                                   >  2:320732/5‑251 (MQ=255)
                 gAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTTC‑CTTCGCCTTTTT‑TTC‑CCCCCcgcgcgcgcccc                                                                                                                                                                                                                                          >  1:606112/1‑247 (MQ=255)
                  aGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTTACTTCGCCTTTTT‑TCC‑CCCCCGGCGCGCGCCCa                                                                                                                                                                                                                                          >  1:71620/1‑250 (MQ=255)
                  aGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTATCATTGCCTTTTT‑TAC‑GCCCCCGCGCGCGACCac                                                                                                                                                                                                                                         >  2:222870/1‑250 (MQ=255)
                     gggCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACa                                                                                                                                                                                                                                      <  1:303126/250‑1 (MQ=255)
                       gCAACCGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑Tc                                                                                                                                                                                                                                   <  1:138574/251‑1 (MQ=255)
                        cAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACCTCGCCTTTTT‑CAC‑CCCCCCGCGCGCGCCCCAACA‑tt                                                                                                                                                                                                                                   >  1:14730/1‑249 (MQ=255)
                          aaCGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTTACTTCGCCTTTTT‑TAC‑GCCCCCgcgcccgggccaacaacacc                                                                                                                                                                                                                                 >  1:170558/1‑235 (MQ=255)
                          aaCGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGAATACGCAGCGGCGGG‑GTCACATCGCCATTTT‑CCC‑Gcccccccgccggagccaaaaccacc                                                                                                                                                                                                                                 >  2:177770/1‑230 (MQ=255)
                               gggCCTACGGCGGCCAGACGTTGACGGGCAAATTGCATACGCGCCACGCTCGCCAGTTTCTGAGCAATAGTTTTACCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGac‑ac                                                                                                                                                                                                                          <  1:70875/251‑1 (MQ=255)
                                     aCGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑GTCACTTCGCCTTTTT‑TAC‑GCCCCGGCGCCCGGCCAAAAA‑ACACCCac‑acctgcac                                                                                                                                                                                                                    >  1:180482/1‑245 (MQ=255)
                                            ccAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CCA‑GCCCCGGCGCGCGCCCAAACA‑TCACCGAC‑ACCGGCCCCATCTGc                                                                                                                                                                                                             >  1:18361/1‑251 (MQ=255)
                                                 cGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTTGCCTTTTT‑TTC‑GCCCCCGCGCGCGACCACAAA‑CCACCCAC‑ACACGCACCATCttcaacac                                                                                                                                                                                                        >  1:144078/1‑244 (MQ=255)
                                                  gTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCCCTTCCCCCTTTT‑TAC‑CCCCCCGCGCGCGACCAAACA‑ACAcccacccttgccccccctgcccctcc                                                                                                                                                                                                        >  1:464853/1‑227 (MQ=255)
                                                    tGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑GTCACTTCGCCTTTTTTCA‑‑GCCCCGGCGCGCGCCCAAAAA‑TCACCGCC‑ACTGGCACCATCTGcacaacc                                                                                                                                                                                                       >  1:113824/1‑246 (MQ=255)
                                                      gcGGGCAAAGGGCAACCGCGCCACGCGCGCCAGTTTCTGGTCGATAGTTTTAACCGTGCCTTGTTCACCCGCCATACGGCGCAGCGAAACTGAGGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGAt                                                                                                                                                                                                   <  1:409758/250‑1 (MQ=255)
                                                       cGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGAtt                                                                                                                                                                                                  <  1:181573/251‑1 (MQ=255)
                                                                  cATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCGTACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACCTCGCCTTTTT‑TTC‑GCCCCCGCGCGCCGCCCAACA‑ACACCGAC‑ACTTGCCCCATcctcccaccccgattttggtttgtct                                                                                                                                                                                       >  1:117594/1‑226 (MQ=255)
                                                                     aCGCGCCACGGGCGCATGTTTCTGAGCGATATTTTTAGCCGGGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGt                                                                                                                                                                                    <  1:414949/251‑1 (MQ=255)
                                                                                gcgcCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCCGCGCGCGACCAAAAA‑TCACCCAC‑ACTGGCACCCTCTGCACATCCCGATTTTGGGTGCTACGGTGCAAATCg                                                                                                                                                                            >  1:421140/1‑248 (MQ=255)
                                                                                         tgtgagctagtgtttagtgcggtacgtgGTCATTCGCCTTACGGCGCGGAAAAACGGGCGGTGAGAAACTCACAGACCTGTCGCGTTCGAGCCACAGGACAACTAAAAATTTCTGTTCGCCGTTAAGCATACGCAGCGGCGAG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCATACA‑TCGCCGGC‑ACTGGCACCATCTGCACATCCAGAGTTGGGTTGCTACGGTGCAAAGCTAGATAGTCGTg                                                                                                                                                                 <  2:234066/226‑1 (MQ=255)
                                                                                                 aTAGTTTTATCCGGGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCtt                                                                                                                                                        <  1:198608/251‑1 (MQ=255)
                                                                                                   aGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTaa                                                                                                                                                      <  1:571067/251‑1 (MQ=255)
                                                                                                     ttttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑GTCACTTCGCCTTTTT‑CAC‑GCCCCCGCGCGCGACCCAACA‑TCACCCAC‑ACCCGCACCATCTGCACCACCAGATTTTGGGTGGTACGGGGCAAATCGAGATAGGCGTGGaaaaagttaat                                                                                                                                                     >  1:257093/1‑241 (MQ=255)
                                                                                                        gatcggagaggggtTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACCTCGCCTTTTT‑TAC‑GCCCCCGCGCCCGACCCAACAACCACC‑AC‑CCTGGCACCCCCTGCCCCACCAGATTTGGGGTGCTACGGTGCAAAACGAGATAATCGTGGAACAACTTAATAGa                                                                                                                                                  >  1:403495/13‑250 (MQ=255)
                                                                                                         acccgtgccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAc                                                                                                                                                 <  1:185326/248‑1 (MQ=255)
                                                                                                          tCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGCGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACCTCGCCTTTTT‑TTC‑GCCCCCGCGCGCGACCACACA‑TCCCCCAC‑ACCCACCCCCTCTCCCAACCCCCAGTTGGGGGGCTACGGTGCACATCGCGATAGTCGTGGAACAGCTTAAAAGAcac                                                                                                                                               >  2:355782/1‑249 (MQ=255)
                                                                                                           ccgcgctgtgtTCATCCGCCGTACGGCGCAGCGAAACTGACGGCGAAAAACGCACAAACCGGTCGCGCCCGAGCCACAGTACATCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGActct                                                                                                                                              <  2:362800/244‑1 (MQ=255)
                                                                                                             ggcccgtgTTCACCCGCCATACGGCGCAGCAAAACTGACGGCGAAAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttc                                                                                                                                            <  2:464853/248‑1 (MQ=255)
                                                                                                                 cgtgTTCACCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTc                                                                                                                                        <  2:14730/251‑1 (MQ=255)
                                                                                                                          ccGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTa                                                                                                                                <  1:468072/250‑1 (MQ=255)
                                                                                                                                 aCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑ATCACTTCGCCCTTTT‑TAC‑GCCCCGCGGCCCGACCCAAAA‑TCTCCCAC‑ACCTGCCCCCTCTGCCCATCCACATTTTGGGTGCTACGGAGCAAATCAAGTTAGGCGTGGGACCGCTTTATAAAATCTTCTTTCCGCGTGTTAT‑TGGTg                                                                                                                        >  1:518576/1‑250 (MQ=255)
                                                                                                                                      gcAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAac‑aca                                                                                                                 <  1:158113/251‑1 (MQ=255)
                                                                                                                                           gAAACTGACGGCGAGAACCGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAAC‑ACACGCgg                                                                                                            <  1:131662/251‑1 (MQ=255)
                                                                                                                                                tGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑CTCACTTTCCCTTTTT‑CAC‑GCCCCCGCGCGGGACCAAAAA‑CCACCCACCAC‑GGCACCCTCTCCACACCCCGATTTTGGGTGCTACGGGGCGAACAGTAAAAGTCGTGGGACAGCCTTATAGACTCTTCTTTTCGCgtgttattagtgcacaaacccggaccgc                                                                                                         >  2:286469/1‑227 (MQ=255)
                                                                                                                                                 gccGGCGAAAAACGCAAAAAACTGTCCCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAAC‑ACACGCGGCccg                                                                                                        <  1:515566/247‑1 (MQ=255)
                                                                                                                                                                  aaaactggagcaccaggcccACAGTACAGAAAAAATTTTCTGTACGCCTATAAGTACCCGCGGCGGCGGG‑TTCCCTTCCCCTTTTC‑CCC‑GCCCCGGCGCGCGACAAAACC‑TCACAGAC‑ACTGGCACAATCTGCAAACCCAGTTTTGGTTTGCTAGGGTGAAAATCGAGATAGTCGTGGAACAGCTAAAAAGACTTTTTTTTCGGATTGAAAT‑AGG‑GAACCACACGCGGCCCGCCGTGAATGAACACATAgc                                                                                     <  2:18361/233‑1 (MQ=255)
                                                                                                                                                                   aaaCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACCTCCCCTTTTT‑TAC‑GCCCCCGCGCGCGACCAAACA‑ACCACGGC‑ACTGGCACCATCTGCACAACCCGACTTGGGGTGCTACGGGGGAAATTGGGATAGTCGTGGAGAAGCTTAATAAAATCTTCTTTCCGCGGGTAAA‑AAGGGAAC‑ACACCCGGCCCGCCGGGAATGAACACATAg                                                                                      >  1:625592/1‑249 (MQ=255)
                                                                                                                                                                                 gAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑GTTACTTTGCCTTTTT‑TAC‑GCCCCGGCGCCCGGACAAACA‑TCCCCCCC‑ACCGGCACCACCTCCACAACCAGATTTTGGGTGCCACCGTGCAAATCGGGGTAGTCCTGGAACAGCTTAATAGACTCCTCTTTCCCGGTGTAATTAGG‑GAAA‑ACACCCCGCCCCCCGTGAATGAAAACATaaccggggagaagcgggc                                                                      >  1:521179/1‑234 (MQ=255)
                                                                                                                                                                                                         gtgtgcccctttaggCATCCGCAGCGGGGGT‑TTCCCTCCGCCTTTTA‑CAC‑CCCCGGGGGCGGGACAAACCA‑ACCCCGCC‑ACTGGCACCATCTGCCCACCCAGAGTGGGGTTGCAACGGTGCGATCCGAGATAGGGGTGGAACAGTTTAATAGACTCTTCTTGCGGGTTTTAAG‑AGGGGACC‑CAACGCGACCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAAc                                               <  2:623880/236‑1 (MQ=255)
                                                                                                                                                                                                                   ttAAGCATACGCAGCGGCGGG‑GTTACTTTGCCTTTTT‑TAC‑GCCCCCGCGCGCGGCCAAACA‑TCACCGGC‑ACTGGCACCATCTGCCCATCCAGCTTTGGGGTGCTACGGTGCAAAACGAGATAGTCGTGGAACAGCTTAATAAACTCTTCTTTCTGCGTGTAAT‑AGGTGAAC‑AAACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCCGCGGCTCTAACGGggcaggca                                     >  1:567828/1‑250 (MQ=255)
                                                                                                                                                                                                                     aaccATACCCAGCGGGGGG‑TTCCCTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCTACAAAACA‑TCCCCGAC‑ACTGGCACCATCTGCCCATCCAGATTTGGTTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGACC‑ACACGCGCCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGt                                  <  1:335642/248‑1 (MQ=255)
                                                                                                                                                                                                                              cAGCGGCGGT‑TTCACT‑CCCCTTTTT‑CCCCGCCCCGGCCCCCGACCAAACA‑TCACCGAC‑ACGGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAG‑AGGTGAAC‑ACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCa                         <  1:402472/251‑1 (MQ=255)
                                                                                                                                                                                                                                           cACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGCTAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAAC‑ACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTAATGTGCCAGCCACTGGgcgc             >  2:459133/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 gCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAAC‑ACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACg       >  2:368365/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   cTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAAC‑ACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTc     >  1:265939/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       tt‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGATTACTAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAAC‑ACACGCGGCCAGCCGTGAATGAACACATAGCGCGGCAGAAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCCACGGCAGc  >  2:201375/1‑250 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                            
ATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTT‑CAC‑GCCCCGGCGCGCGACCAAACA‑TCACCGAC‑ACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAAC‑ACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGC  >  NC_000913/4255813‑4256308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑