Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0610GA25.6% 88.7 / 11.3 43intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base G (29/3);  new base A (0/11);  total (29/14)
Fisher's exact test for biased strand distribution p-value = 6.33e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTT  >  NC_000913/1824813‑1825307
                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                        
gatgCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGagaaaagaa                                                                                                                                                                                                                                                        <  1:137637/251‑1 (MQ=255)
                    gTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCAcc                                                                                                                                                                                                                                    >  2:115060/1‑251 (MQ=255)
                             aCGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGAAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGACCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCCACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GC‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGAATTACTg                                                                                                                                                                                                                           >  1:539127/1‑251 (MQ=255)
                               gTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                                                                                                                          >  1:125944/1‑250 (MQ=255)
                                   tGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGAGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACATCTGTTT‑GA‑GAAGAGAAAAGACAACAGCCGATCCTGTCCACCGCCTTACTGCCAGGt                                                                                                                                                                                                                     >  2:50020/1‑251 (MQ=255)
                                       ggTGGGCACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTGCGCCACAGATTCACCGACATTTTACGCATATCAAAGAATTCCATACTC‑GCTCCCGGCCCGTTCTTTTAATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACAGCATTACTGCACGGTAGTg                                                                                                                                                                                                                 >  2:487294/1‑251 (MQ=255)
                                       ggTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                                                                                                                                 >  1:121361/1‑251 (MQ=255)
                                       ggTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                                                                                                                                 >  1:61874/1‑251 (MQ=255)
                                       ggTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                                                                                                                                 >  1:295001/1‑251 (MQ=255)
                                           ggAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTCCGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAAAAAAGAAACCCCCCGACCTTGTCCACCGCTTTACTGCaggaaaggggac                                                                                                                                                                                                              >  2:54007/1‑239 (MQ=255)
                                            gAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATATTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GT‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                                                                                                                             >  2:2934/1‑250 (MQ=255)
                                                   cAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                                                                                                                                     >  1:330447/1‑251 (MQ=255)
                                                                 ttCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                                                                                                                               >  1:399803/1‑243 (MQ=255)
                                                                         cagctttcccccACAGATTCCCCGATATTTCACGCATAACAAATTTTTCCATCCTC‑GCTCCCGGCTTGTTCTTTTCATTGTTATCGTTCAGGTACCTGGGGTTGGTTATCGGCAACTGGGGTGATTTACGTTAGTGTTGATCAGGTTTTTTTT‑ACAACTTTTT‑GA‑GAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaa                                                                                                                                                                               <  1:40586/241‑1 (MQ=255)
                                                                                 cGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCACGGCCCGTTCTTTTCATTGATCTCGTTCAGGTATCCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGCGGTGTTCCGGCTTTGTTT‑ACAACTGTTT‑GA‑GGAGAGAAGAGAAAACCGCCGATCCTGTACACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGACTTATGGTTTTTgtgtgaacgagtctt                                                                                                                                                                       >  2:298159/1‑237 (MQ=255)
                                                                                             aCCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGGTTAAAAATTATT‑ctgcaggttccg                                                                                                                                                          >  2:631331/1‑245 (MQ=255)
                                                                                               cGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGGTGCAggg                                                                                                                                                        >  1:358503/1‑250 (MQ=255)
                                                                                                          gCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCttt                                                                                                                                             >  1:94108/1‑251 (MQ=255)
                                                                                                               acAAATTATTCCAT‑CTCCGCTCCCGCCCTTTTTTTTTTATTGTTATCGTTAAGGTACCCGTGGATGGTTATCGGCAACTCGGTTTATTTACTTTGGTGGTGATCAGCCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCg                                                                                                                                        <  1:279516/250‑1 (MQ=255)
                                                                                                                     tATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGCTTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTTTT‑CCGCAGTTGCAGGAATTTTAACTTTTGCCGCTGGa                                                                                                                                   >  1:546689/1‑250 (MQ=255)
                                                                                                                     tATTCCATACTC‑GATCCCAGCACGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGCTTTACGCTAGTGGTGTTCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGCGAAAAGAAAACCGCCGATCCTGTCAACCGCATTACTGCACGGTAGTGGACAAGACCGGCGGCATTCAGGTTTTTTACTTCAAGGTTATTGCA‑CAGTTGCAGGCATTTTACCTTTTTCCGCTggg                                                                                                                                   >  2:484177/1‑249 (MQ=255)
                                                                                                                                  cctcccggcctttttttttttttGTTATCGTCCAGGTACCCGGGGATGTTTTTCGGCATCTGGTGTGATTTACTTGAGGGGTAATGAGGTTTTTTTT‑ACA‑TTGTTTTGA‑AAAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGAc                                                                                                                     <  1:356575/230‑1 (MQ=255)
                                                                                                                                          cccttttttttCATTGTTTGCGTTCGGGTAGCGGTGGTGGGTTTCCGGCACCTCGGGTGATTTATGTTGGGGGGGATC‑GGCTTTTTTTTACAATTTTTG‑GA‑AAAGAGAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCa                                                                                                             <  1:120361/244‑1 (MQ=255)
                                                                                                                                                     cATTGTTATCGTTGGGGTAACGGTGAATGTTTATCGGCAACTCGGGTTTTTTACGTATGTGGTAATCAGTTTTTTTTT‑ACACCT‑TTT‑GAAGAAGAAAAAAAAAAACCGCCGATCCTGGCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCAtt                                                                                                  <  1:163473/251‑1 (MQ=255)
                                                                                                                                                              tgttggggTAACGGTGTATGGTTACCGGCAATTCGGGTGATTTGCGTTGGTGGTGAGCAGGTTTTTTTT‑AAAATTGTTG‑GA‑GAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCt                                                                                          <  1:177393/244‑1 (MQ=255)
                                                                                                                                                                ttCAGGTACCGGTGGATGTTTCTCGGCACCGGGGGTGATTTACTTTAGTGGTAATCAGTTTTTTTTA‑ACATTTGTGG‑GA‑AAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCttt                                                                                        <  1:520442/250‑1 (MQ=255)
                                                                                                                                                                    ggTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTTGCGCTGGACGTTTTGTC‑CGACGCTTCTCAAAATTTGCATTAAATTGCtttttttt                                                                                   >  2:398718/1‑248 (MQ=255)
                                                                                                                                                                                 ggttaccggaatctggggTGTTTTACGTTAGTGGTGATCAGTTTTTTTTT‑ACAACTGTTG‑AA‑AAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGAATTTTACCTTTTGCCGATGGAAGTTATGTG‑CCAGGCAGCGCCGAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAg                                                                       <  1:582885/235‑1 (MQ=255)
                                                                                                                                                                                           aaCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGGTTTTTTGCTGAACGGATATT‑TAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGGCGTTCTTTC‑AGACGCTTTTCCAAATTATCCTTAAATTTCTTTTTCTTTTTCGGCGTCCGGAtgttgaaa                                                             >  2:151909/1‑247 (MQ=255)
                                                                                                                                                                                                  gTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGATGGACGTTCTGTC‑AGACGCTTCTCAAAATTATCATTAAATTGCTTTTTCTTTTCCGGCGTCAGGCTGTTGTTAATTTTGtt                                                     >  2:134614/1‑251 (MQ=255)
                                                                                                                                                                                                    gttttcggttagtgggcaccagtcTTTTTTT‑ACAACTTTTT‑GA‑AAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTc                                                    <  1:343173/227‑1 (MQ=255)
                                                                                                                                                                                                        tctgtgaggggggatcgtcttttttta‑ACATCTGTTA‑GA‑GAAGAAAAAAAAAAACCGCCGATCCTGGCCACCGCATTACTGCAAGGGAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTggg                                                <  1:586333/230‑1 (MQ=255)
                                                                                                                                                                                                        tACGTTAGTGGTGATCAGGCTTTTTTT‑ACAAATGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGAAGGCATTTTACCTTTTTCCGCTGGACGTTCTTTC‑AGACGCTTCTCCAAATTAGCATTAAATTGCTTTTTCTTTTCCGGCGTCCGGATGTTGTAAATTTTTTTCTGGGt                                               >  1:171394/1‑251 (MQ=255)
                                                                                                                                                                                                           gTTAGTGCTGAACAGGATTTTTTT‑CCAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAAACGACCGGCGGATTTAAGTTTTTTGGGTGAAAGAATAAT‑CAGTAATTGCAGGCATTTTACCTTTTGCCGCCGGAAGTTC‑GTCCAAAAGCCTCTCAAAAATTGCATTAAATTGCTTTTTCTTTTCACGCGTCAGGCAGATGTAATTTTTGtttttgttttt                                            >  2:536041/1‑242 (MQ=255)
                                                                                                                                                                                                               gtggtgATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCCGAAAAATTATT‑CAGCAGTTGCAGGCCTTTTACCTTTTTCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAAATTTGTTCTGGGTTTTCAtt                                        >  1:287396/1‑250 (MQ=255)
                                                                                                                                                                                                                         gggtttttttttcatttGTTT‑GA‑GAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTACATGTGCGCCAGc                               <  1:247611/235‑1 (MQ=255)
                                                                                                                                                                                                                              ttttt‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTTCCGGCATTTTATCTTTTTCCTCTGGGCGTTTTGTC‑AGACGCTTCTCAAAATTAGAATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTTTCAATTTTGTTTTGGGTTTCTATGTGCGCCAGCCTGttt                         >  2:55981/1‑250 (MQ=255)
                                                                                                                                                                                                                              ttttt‑AAAACTGTTT‑GA‑AAAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGtt                          <  1:596441/250‑1 (MQ=255)
                                                                                                                                                                                                                                  t‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCtt                     >  1:285530/1‑251 (MQ=255)
                                                                                                                                                                                                                                    aaaaCTGTTT‑GA‑AAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCttt                    <  1:631331/249‑1 (MQ=255)
                                                                                                                                                                                                                                    aCAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCCTTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCttt                    >  1:192082/1‑251 (MQ=255)
                                                                                                                                                                                                                                      aaCTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTgc                  >  1:429176/1‑251 (MQ=255)
                                                                                                                                                                                                                                           gtttagaaaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCt               <  2:285530/243‑1 (MQ=255)
                                                                                                                                                                                                                                                aaaaaaaaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGttct          <  1:50020/243‑1 (MQ=255)
                                                                                                                                                                                                                                                   aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGGTGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTcc       >  1:100663/1‑251 (MQ=255)
                                                                                                                                                                                                                                                        aaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCAtttt  >  1:277927/1‑251 (MQ=255)
                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                        
GATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTC‑GCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTT‑GA‑GAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT‑CAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTT  >  NC_000913/1824813‑1825307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑