breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 105 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,2680TC36.4% 78.5 / 73.9 78intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_000913433,6340TG32.8% 144.7 / 14.3 123G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase
*NC_0009132,326,4500TG32.8% 260.9 / 29.5 122G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_0009132,434,8820TG31.7% 215.0 / 26.2 106Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
*NC_000913827,5070TG31.6% 302.2 / 16.1 133D492A (GAC→GCC) ybhFABC exporter ATP binding subunit YbhF
*NC_0009131,292,4580TA31.1% 89.1 / 12.0 45intergenic (+93/+51)galU/hnsUTP‑‑glucose‑1‑phosphate uridylyltransferase/DNA‑binding transcriptional dual regulator H‑NS
*NC_000913811,7210TG31.0% 245.6 / 16.9 146V67G (GTG→GGG) bioCmalonyl‑acyl carrier protein methyltransferase
*NC_000913786,8550AC30.5% 280.0 / 15.8 118G247G (GGT→GGGgpmA2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase
*NC_0009132,185,1990TG30.5% 359.0 / 39.0 175T35P (ACC→CCC) thiMhydroxyethylthiazole kinase
*NC_0009132,228,2750TC29.9% 136.0 / 13.2 78intergenic (‑1005/‑707)yohF/yohPputative oxidoreductase YohF/putative membrane protein YohP
*NC_0009134,256,0690GC29.9% 163.8 / 22.9 77P133R (CCG→CGG) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase
*NC_0009133,531,4400AC28.8% 386.1 / 13.8 158Y334D (TAT→GAT) yhgEputative transport protein YhgE
*NC_0009134,457,1050AG28.7% 303.3 / 27.7 139I441V (ATC→GTC) mplUDP‑N‑acetylmuramate‑‑L‑alanyl‑gamma‑D‑glutamyl‑ meso‑2,6‑diaminoheptanedioate ligase
*NC_0009132,664,8960TG28.5% 336.2 / 23.1 138G169G (GGT→GGGyfhRputative peptidase
*NC_0009133,275,6110AC28.2% 183.2 / 13.4 85E110D (GAA→GACgarDGarD
*NC_0009134,056,1150AC28.2% 275.3 / 18.3 119G75G (GGT→GGGglnLprotein histidine kinase NtrB
*NC_0009134,287,7190TA28.0% 213.7 / 41.5 100intergenic (+52/‑45)ytcB/nrfAprotein YtcB/cytochrome c552 nitrite reductase
*NC_0009131,015,5160GT27.8% 158.3 / 15.9 72intergenic (+57/‑199)pqiC/rmfintermembrane transport lipoprotein PqiC/ribosome modulation factor
*NC_0009132,395,4020AC27.6% 356.1 / 20.9 146G165G (GGT→GGGnuoJNADH:quinone oxidoreductase subunit J
*NC_0009132,745,0890AC27.3% 296.3 / 21.6 122V84G (GTG→GGG) trmDtRNA m(1)G37 methyltransferase

Marginal new junction evidence (lowest skew 10 of 12 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1411899 =114 (0.900)5 (0.040) 5/426 10.5 4.7% coding (50/936 nt) ttcA tRNA cytosine(32) 2‑sulfurtransferase TtcA
?NC_000913 = 1434958 101 (0.890)intergenic (‑552/+227) ynaM/uspF protein YnaM/universal stress protein F
* ? NC_000913 4295832 =113 (0.890)3 (0.030) 3/400 11.4 2.6% intergenic (+38/+604) gltP/yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
?NC_000913 = 4296394 131 (1.230)intergenic (+600/+42) gltP/yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
* ? NC_000913 = 2733016160 (1.260)3 (0.020) 3/478 12.5 1.9% coding (714/2130 nt)
coding (1158/2574 nt)
clpB
clpB
chaperone protein ClpB
chaperone protein ClpB
?NC_000913 3752913 = 142 (1.120)coding (922/975 nt) yiaU putative DNA‑binding transcriptional regulator YiaU
* ? NC_000913 = 1811315151 (1.190)3 (0.020)
+C
3/476 12.5 2.0% coding (382/1392 nt) tcyP cystine/sulfocysteine:cation symporter
?NC_000913 = 4014805 138 (1.090)coding (1753/2262 nt) metE cobalamin‑independent homocysteine transmethylase
* ? NC_000913 1104384 =92 (0.730)3 (0.020) 3/478 12.5 2.8% coding (434/456 nt) csgB curlin, minor subunit
?NC_000913 1708463 = 117 (0.920)coding (1545/2223 nt) rsxC SoxR [2Fe‑2S] reducing system protein RsxC
* ? NC_000913 898481 =90 (0.710)3 (0.020) 3/478 12.5 2.4% intergenic (+4/‑37) ybjO/rlmC putative inner membrane protein/23S rRNA m(5)U747 methyltransferase
?NC_000913 = 3344179 149 (1.170)coding (236/726 nt) lptB lipopolysaccharide transport system ATP binding protein LptB
* ? NC_000913 = 616210149 (1.170)3 (0.020)
+G
3/476 12.5 2.1% coding (2054/3882 nt) entF apo‑serine activating enzyme
?NC_000913 = 1465821 137 (1.080)pseudogene (430/2518 nt) ydbA putative outer membrane protein, N‑terminal fragment
* ? NC_000913 = 447262142 (1.120)3 (0.020) 3/478 12.5 1.9% coding (444/891 nt) cyoE heme O synthase
?NC_000913 3626058 = 169 (1.330)coding (746/1125 nt) yhhJ ABC transporter family protein YhhJ
* ? NC_000913 = 442737142 (1.120)3 (0.020) 3/478 12.5 2.1% coding (1189/1449 nt) thiI tRNA uridine 4‑sulfurtransferase
?NC_000913 1263044 = 134 (1.060)coding (457/624 nt) lolB outer membrane lipoprotein LolB
* ? NC_000913 418803 =141 (1.110)3 (0.020) 3/478 12.5 2.0% coding (915/1296 nt) phoR sensor histidine kinase PhoR
?NC_000913 2812840 = 155 (1.220)coding (225/1539 nt) emrB multidrug efflux pump membrane subunit EmrB