breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 47 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,228,2750TC41.7% 44.9 / 15.4 48intergenic (‑1005/‑707)yohF/yohPputative oxidoreductase YohF/putative membrane protein YohP
*NC_0009132,326,4500TG40.4% 82.2 / 12.6 53G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_0009132,228,2390GC35.3% 62.4 / 14.0 51intergenic (‑969/‑743)yohF/yohPputative oxidoreductase YohF/putative membrane protein YohP
*NC_0009132,096,0900AG34.4% 125.8 / 11.8 64I83V (ATT→GTT) hisFimidazole glycerol phosphate synthase subunit HisF
*NC_000913179,0260AC34.2% 149.8 / 11.5 84V43G (GTT→GGT) mtn5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase
*NC_0009134,296,2680TC34.2% 47.7 / 32.8 41intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_000913954,3880CT33.3% 43.8 / 11.4 42A27T (GCA→ACA) focAformate channel FocA
*NC_0009134,604,3500CT32.6% 77.8 / 15.7 49S64F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009133,465,1670GT31.1% 67.3 / 17.5 46C29F (TGT→TTT) gspMType II secretion system protein GspM
*NC_0009133,497,7280AG30.3% 148.9 / 21.3 76G242G (GGA→GGGnirCnitrite transporter NirC
*NC_0009131,435,7180GA30.0% 93.6 / 16.1 51intergenic (‑99/+42)uspF/ompNuniversal stress protein F/outer membrane porin N
*NC_0009132,434,8820TG29.6% 187.2 / 12.2 82Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
*NC_0009131,701,3030TC29.3% 143.6 / 19.4 77I116T (ATC→ACC) malYnegative regulator of MalT activity/cystathionine beta‑lyase
*NC_0009133,556,2070TC27.9% 132.9 / 14.6 61S215G (AGT→GGT) rtcARNA 3'‑terminal phosphate cyclase
*NC_0009131,822,1720CT27.6% 112.4 / 18.5 58D29N (GAC→AAC) osmEosmotically‑inducible lipoprotein OsmE
*NC_000913213,3410AG27.4% 141.5 / 13.0 62G337G (GGA→GGGtilStRNA(Ile)‑lysidine synthetase
*NC_0009131,383,7220GA27.3% 75.2 / 10.1 44F80F (TTC→TTTycjWDNA‑binding transcriptional repressor YcjW
*NC_0009132,878,9470CT26.9% 98.2 / 10.1 52E276K (GAA→AAA) cas1multifunctional nuclease Cas1
*NC_0009134,604,3440CT26.5% 89.5 / 12.9 49S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009131,435,7270GA26.4% 108.8 / 20.2 53intergenic (‑108/+33)uspF/ompNuniversal stress protein F/outer membrane porin N

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1411899 =69 (0.880)6 (0.090) 6/442 6.0 9.0% coding (50/936 nt) ttcA tRNA cytosine(32) 2‑sulfurtransferase TtcA
?NC_000913 = 1434958 60 (0.850)intergenic (‑552/+227) ynaM/uspF protein YnaM/universal stress protein F
* ? NC_000913 2057851 =43 (0.550)3 (0.040)
+C
3/492 8.0 5.5% intergenic (+277/+176) yeeN/asnW putative transcriptional regulator YeeN/tRNA‑Asn
?NC_000913 = 2100961 61 (0.780)coding (308/1407 nt) gnd 6‑phosphogluconate dehydrogenase, decarboxylating
* ? NC_000913 972454 =100 (1.270)3 (0.040) 3/494 8.1 3.0% coding (703/894 nt) ycbJ putative phosphotransferase YcbJ
?NC_000913 3140048 = 96 (1.220)coding (271/342 nt) hybF hydrogenase maturation protein HybF
* ? NC_000913 = 96160197 (1.230)3 (0.040) 3/494 8.1 3.0% coding (401/684 nt) cmk cytidylate kinase
?NC_000913 = 2832103 96 (1.220)coding (187/1515 nt) norR DNA‑binding transcriptional dual regulator NorR
* ? NC_000913 498700 =82 (1.040)3 (0.040) 3/494 8.1 4.1% coding (646/963 nt) hemH ferrochelatase
?NC_000913 = 3222259 57 (0.720)coding (794/984 nt) ebgR DNA‑binding transcriptional repressor EbgR
* ? NC_000913 = 23271569 (0.880)3 (0.040) 3/494 8.1 3.5% coding (1241/1359 nt) mltD membrane‑bound lytic murein transglycosylase D
?NC_000913 = 3844758 97 (1.230)coding (795/1767 nt) adeD adenine deaminase