Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,228,2750TC41.7% 44.9 / 15.4 48intergenic (‑1005/‑707)yohF/yohPputative oxidoreductase YohF/putative membrane protein YohP
Reads supporting (aligned to +/- strand):  ref base T (16/12);  new base C (6/14);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 8.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCGCCCGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTAACCTCCGCCC‑GATTCTTT‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCG  >  NC_000913/2228027‑2228524
                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                   
gcCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCGCCCGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTAtcctcc                                                                                                                                                                                                                                                                   <  1:312862/251‑1 (MQ=255)
   gggTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTG‑CCTGC‑G‑TTCC‑GCCCCCCGTGCTTTAACC‑GTAACCAGGCCGGCA‑GGAACCTccccc                                                                                                                                                                                                                                                                >  1:414709/1‑248 (MQ=255)
     gTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCGCCCGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTAACCTCCGCCC‑g                                                                                                                                                                                                                                                             <  1:267506/251‑1 (MQ=255)
         tGCTGGCATTAATATCGGTTTCCACACCTTCAACAGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCGCCCGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTAACCTCCGCCC‑GAtt                                                                                                                                                                                                                                                          <  2:160219/250‑1 (MQ=255)
          gCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCGCCCGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTAACCTCCGCCC‑GATTc                                                                                                                                                                                                                                                         <  2:465984/250‑1 (MQ=255)
             ggCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCGCCCGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTAACCTCCGCCC‑GATTCttt                                                                                                                                                                                                                                                      <  1:571621/250‑1 (MQ=255)
                   aaTATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCGCCCGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTAACCTCCGCCC‑GATTCTTT‑CCCCA‑Ga                                                                                                                                                                                                                                             <  1:465979/251‑1 (MQ=255)
                             tCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCGCCCGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTAACCTCCGCCC‑GATTCTTT‑CCCCA‑GATATCGAG‑At                                                                                                                                                                                                                                   <  1:301229/250‑1 (MQ=255)
                                          cTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTT‑CTCGC‑G‑TTCC‑GCCGCCCGGGCTTTAACC‑GTACCCAGGCGGGCA‑GTAAACCCCCCCC‑CATTCTTT‑CCCCC‑GATATCGAG‑GTTCCAGccccccctt                                                                                                                                                                                                                     >  1:139551/1‑245 (MQ=255)
                                            gaagaaGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTT‑CTCGC‑G‑TTTC‑CCCGCCCCCGCTTTAACC‑CGACCCCCGCGGGCA‑GTAACCCCCCCCC‑CCTTCTTT‑TCCCC‑GATATCCAG‑ATGCCACCccgccggta                                                                                                                                                                                                                    >  1:144251/1‑247 (MQ=255)
                                              agaagTAACCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGAAAGCCCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCGCCCGTGTTTTAACC‑GTCCCCAGGCGGGCA‑GTACCCTCCGCCC‑GATTCTTT‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGc                                                                                                                                                                                                                 <  1:8413/251‑1 (MQ=255)
                                               gaagTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCGCCCGTGCTTTAACC‑GTCCCCAGGCGGGCA‑GTACCCCCCGCCC‑GATTCTTT‑CCCCA‑AATATCGAG‑ATGCCAGCCCGCCGTTAAGcc                                                                                                                                                                                                                <  1:346023/251‑1 (MQ=255)
                                                            tCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTG‑CTCGC‑G‑GTCC‑CCCGCCCCCGCTTTTACC‑GGACCCATGCGGGGA‑GTAAACTCCGACC‑CATTCTTT‑CCCCC‑GATAACGAG‑AAGCCCGCACCCCGCTAAGGC‑AAAAATACC‑gctg                                                                                                                                                                                                 >  1:111521/1‑248 (MQ=255)
                                                               gATATAGCTGGCGATCGGTCGCGATAATGGAGTTCTGCTCTTTTTCTATTTGATGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGATACGCTGCCAGCCAGCAATTGGCGGGGTTG‑CTCCC‑G‑TTCC‑GCCCCCCGTGCCTTTACC‑GCACCCCGGGGGGGA‑GGAACCTCCGCCC‑GAGTCTTT‑CCCCA‑GAAATCGAG‑ATGCCAGCCCCCCGGTAAGCCCAAAAgacccttgggg                                                                                                                                                                                                >  1:500952/1‑241 (MQ=255)
                                                                   taGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTG‑CTCGC‑GGTTCC‑‑CCGCCCGGGCTTTTAAC‑GTACCCCAGCGGGCA‑GTAAACCCCCCCC‑GCTTTTTT‑CCCCC‑GATATCGAG‑ATGCCAGCCCGCCCTTAAACC‑CAAAATACC‑CTTGGGGTA‑cc                                                                                                                                                                                         >  1:611498/1‑251 (MQ=255)
                                                                               ggtcgcattaatgtggtttggctttttttCTAGTTGCGGCAAGACCG‑GTTTAAACGCCGCC‑GGGGTTG‑GCACCCCCTAGTACGGGCGGGCTACGCGGCCAGCCAGCAAT‑GGGGGGTTTG‑‑GCGCTG‑TTCCCGCCCCCCGTTCTTGAACT‑GCCCCCAGG‑GGGAACGTATCCCCCGCCC‑GATT‑TTT‑CCCCAAGTTACGGAG‑AGCCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCg                                                                                                                                                                             <  2:135951/227‑1 (MQ=255)
                                                                                      aTAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCCCCCGGGCTTTAACC‑GTACCCAGGCGGGCA‑GTAACCTCCGCCC‑GATTCTTTTCCCCA‑‑AAAACGAG‑ATGCCAGCCCCCCGTTTAGCC‑CAAAGTACA‑GTTGGGGTA‑CCACCGGCCGGGCGTACCTGc                                                                                                                                                                      >  1:136122/1‑251 (MQ=255)
                                                                                        aaTGGTTTTCTGCTCTTTTTCAATTGGCGGCAAGCCCGTGTTTAC‑CGCCGCCTGGGTTTG‑CCCCCCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCGCCCGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTACCCTCCGCCC‑GATTCTTT‑CCCCA‑GATAGCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCgg                                                                                                                                                                    <  1:364547/251‑1 (MQ=255)
                                                                                         aTGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTG‑CTCGC‑G‑TTCC‑GCCCCCCGTGGTTTAAAC‑GTACCCAAGCGGGCA‑GTAACCCCCGCCC‑GATTTTTT‑TCCCC‑GATATCGAG‑ATGCCAACCCGCCGTTAAGCC‑AAAAGTAAC‑GTTGGTGTA‑CCCCGGGCCGGTCGTACCCGCGGc                                                                                                                                                                   >  1:556319/1‑251 (MQ=255)
                                                                                           ggttttttGCTTTTTTTTTATTTCCTGCAAGCCCGTTTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCAACGCTGCCAGCCAGCAATGGGCGGTTTTG‑CTCGC‑G‑TTCC‑GCCGCCCGGGCTTTACCC‑GTACCCAGGCGGGCA‑GTAACCCCCGCCC‑GATTCTTC‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGcc                                                                                                                                                                  <  1:304554/243‑1 (MQ=255)
                                                                                              gttcgtctctTTTTCTTTTTGCTGAAAGCCCGTGTTTCC‑CGCCTCGTGGTTTTC‑CCACCCCC‑AGTACAGGTGGGTTCCGCTGCCAGCCAGAATTTGGCGGGTTTG‑CTCCC‑G‑TCCC‑GCCCCCCTTGTTTTAACC‑TCACCCAGGCGGGAA‑GTAACCCCCCCCC‑GATTCTTC‑CCCCA‑GAGTTCGAG‑ATGCCCGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGa                                                                                                                                                               <  2:383311/244‑1 (MQ=255)
                                                                                                tCTGCTCTTTTTCTATTTGCTGAAAGACCGTTTTTAA‑CGCCGCC‑GGGTTTGGCCCCCCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑CCCGCCCGTGTTTTAACC‑GTACCCAGGCGGGAA‑GTACCCCCCGCCC‑GATTCTTC‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATc                                                                                                                                                             <  1:441837/250‑1 (MQ=255)
                                                                                                    ttctTTTTCTATTTGCTGAAAGACCTGGTTTAC‑CGCCGCCTGGGTTTG‑CCCCTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CGCGC‑G‑TTCC‑GCCCCCCGTGCTTTAACC‑GTCCCCAGGCGGGCA‑GTACCCCCCGCCC‑GATTCTTC‑CCCCA‑GATATGGAG‑ATGCCGGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTc                                                                                                                                                        <  1:402575/249‑1 (MQ=255)
                                                                                                                             gtgtTTAC‑CCCCGCCTGGGTTTC‑CCCCCCCC‑AGTACAGGCGGGCTACGCTCCCAGCCAGCATTTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCCCCCGTGCTTTACCC‑GTCCCCAGGCGGGAA‑GTACCCTCCCCCC‑GATTCTTC‑CCCAA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTc                                                                                                                              <  1:41384/251‑1 (MQ=255)
                                                                                                                                    a‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTT‑CTCGC‑G‑TTTC‑GCCCCCCGGGCTTTAAAC‑GGAACCAGGCGGGCC‑GGAACCCCCGGCC‑GATTCTTT‑TCCCA‑GATATCGAG‑GAGCCAACCCGCCGGTAAACC‑AAAAGGACCCGTTGGGGAA‑CCA‑GGGCCGGTCGGACCTGCGGCCGGATCG‑GTCAGAGCAAAAGGCCCCATTACACCTTCTGCC‑gcc                                                                                                                      >  1:490508/1‑249 (MQ=255)
                                                                                                                                       gccgccGGGTTTTG‑CCACCCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGTTTTG‑CGCGC‑G‑TTCC‑GCCGCCCGTGCTTTAACC‑GTCCCCAGGCGGGCA‑GTAACCTCCGCCC‑GATTCTTT‑CCCCA‑GATACCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑t                                                                                                                   <  1:469150/251‑1 (MQ=255)
                                                                                                                                        ccgctggggTTTG‑CCTCCCCC‑AGTACGGGCGGGTTACCCCGCCCGCCAGCAATGGGCGGGTTGG‑CTCGC‑G‑TCCC‑GCCCCCCGTGCTTTAACC‑GTCCCAAGGCGGGAA‑GTACCCCCCGCCC‑TATTCTTC‑CCCCA‑GATATCGAG‑TGGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑tt                                                                                                                  <  2:380196/245‑1 (MQ=255)
                                                                                                                                         ccgccggggttgccctccccc‑AGTACGGGCGGGCTCCGCTCCCGGCCAGCAATTGGCGGTTTTG‑CCCGC‑T‑TCCC‑CCCCCCCGTGTTTTAACC‑GTCCCGGGGGGGGCA‑GTACCCCCCCCCC‑AATTCTCT‑CCCAA‑GATACCGAG‑AGGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑tt                                                                                                                  <  2:233274/233‑1 (MQ=255)
                                                                                                                                             tGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTG‑CTCGC‑G‑GTTC‑GCCGCCCGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTAACCCCCGCCC‑GGTTCTTT‑TCCCA‑GATATCCGG‑ATGCCAGCCCGCCGGTAAGCC‑CAAAGTACC‑GTTGGTGGA‑CCCCCGGCCGGGCGGACCTGCGGCCGGGTCG‑GTCAGAACAAAACGCCCCCTTAAGCCTTCCGCC‑GACA‑ttttttt                                                                                                             >  1:616046/1‑250 (MQ=255)
                                                                                                                                                    g‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCGCCCCTGCTTTTACC‑GTACCCCGGCGGGCA‑GGAAACTCCGCCC‑CGTTCTTT‑CCCCC‑GATATCGAGCATTCAACCCCCCCGT‑AAGCC‑AAAAATACC‑GCTGGTGTA‑CCACGGGCCGGGCGTGGCTCCGGCCGGGGCGGTTC‑GCGCAAACGGCCCCCCTAAGCCCTCTCCCCGAC‑‑TTTTTTGCCGCTcc                                                                                                      >  2:36879/1‑251 (MQ=255)
                                                                                                                                                              gataacgggCGGGCAACGCTGCCGGCCAGCACTGGGCGGGTTTG‑CCCGC‑G‑TTCC‑GCCCCCCGTGCTTTAACC‑GTACCCGGGCGGGCT‑GTAACCCCCGCCC‑GATTCTTT‑CCCAA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATccgc                                                                                              <  1:389343/244‑1 (MQ=255)
                                                                                                                                                               agaacaggcgggttcccgtGCCCGCCAGCATTGGGCGGTTTTC‑CTCGC‑T‑TCCC‑CCCGCCCGTTCTTTAACC‑GTCCCCGGGGGGGCA‑TTACCCCCCGCCC‑GTTTTTTC‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATccgcc                                                                                             <  2:611498/233‑1 (MQ=255)
                                                                                                                                                               aGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTG‑CTCGC‑G‑GTCC‑CCCGCCCGCGCCTTAAAC‑GGAACCCGGCGGGCA‑GTAACCTCCGCCC‑GGGTCTTT‑CCCCC‑GATATCGAG‑CTGCCAGCCCCCCCTTAAAAC‑CAAAGAACC‑CGAGGGGTA‑ACACCGGGCGGGCCTAACCGCGGCCGGATAG‑GTGAGAGCAAACGGCCCCAATAAGCCCTCTGCC‑GAAA‑TTTTTTTCCGcctcctatccggc                                                                                             >  1:483337/1‑239 (MQ=255)
                                                                                                                                                                     ggggggCTACGCTGCCACCAAGCATTTGGGGGTTTTC‑CTCGC‑G‑TTCC‑GCCCCCCTTGCTTTACCC‑GTCCCCAGGCGGGCA‑GTAACCCCCGCCC‑GTTTCTTC‑CCCCA‑GATACCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAg                                                                                       <  1:18779/248‑1 (MQ=255)
                                                                                                                                                                      gCGGGCTACGCGGCCAGCCGGCAATGGGGGGGTTTG‑CCCGC‑T‑TCCC‑GCCGCCTGTG‑TTTAACCCGTCCCCGGGCGGGAA‑GTACCCCCCCCCA‑GATTCTTC‑CCCCA‑GATACCGAG‑GTGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGt                                                                                      <  2:422415/251‑1 (MQ=255)
                                                                                                                                                                         ggCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑ATCGC‑G‑TTCC‑CCCGCCCGTGCTTTAAAC‑GTACACAGGCGGGCC‑GTAACCTCCGCCC‑CATTCTTT‑CCCCA‑GGTATTGAG‑ATGCCCGCCCGCCGGTAAGCC‑AAAAATACC‑GTTGG‑GTAACCACGGGCCGGTCGACCCTTCGGCCGGCACG‑TTCAGCGCAAACCACACCATTATGCCTTCTGCC‑GACC‑TTTTTTTGCGCTCCATCTCCACCGACAAGTCg                                                                                    >  2:530722/1‑250 (MQ=255)
                                                                                                                                                                              ggCTGCCGGCCAGCAAGGGGCGGTTTTC‑CCCGG‑T‑TTCC‑CCCCCCCGTGTTTTACCC‑GTGCCCAGGTGGGCG‑GAACCCTCCCCAC‑GATTCTTC‑CCCCA‑GATTTGGGG‑TTCCCCCGCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGTTCG‑TTCAGAGCAAACGGCCCGATTAAGCCTTCTGCC‑GACA‑TGTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGc                                                                              <  1:504021/250‑1 (MQ=255)
                                                                                                                                                                                                   gggTTTGTCTCGC‑G‑T‑CC‑CCCCCCCGTTCTTTAACCCGT‑CCCAGGGGGGCC‑ATAACCCCCCCCCCGTTTCTTC‑CCCAA‑AATATCGAG‑ATCCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAAc                                                          <  2:139551/251‑1 (MQ=255)
                                                                                                                                                                                                    ggTTTG‑CTCGC‑G‑TTCC‑GCCGCCCGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTAACATCCGCCC‑GATTCTTT‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGATAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGACCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACTGACCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCCTCTCCGCCGTAAAGTCGATCTGCGGCCCATCCTGGGTGGCACCTg                                                        >  2:108903/1‑251 (MQ=255)
                                                                                                                                                                                                                   ttCC‑GCCGCCCGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTAACCTCCGCCC‑GATTCTTT‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATCCGCCGCAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCCCCTgtg                                            >  2:347828/1‑249 (MQ=255)
                                                                                                                                                                                                                     cc‑gccgCCCGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTAACCTCCGCCC‑GATTCTTT‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GAAC‑TTTTTTGCCGCTCCCTATCCGCCAAAAAGTCGATCTGCGGACCCTCCTGAGTGGCAACTGACTTCGCCGGGGCt                                          >  2:53434/1‑251 (MQ=255)
                                                                                                                                                                                                                      cccccccccgtctttaaaCC‑GTCCCCAGGCTGGCC‑GTACCCCCCCCCC‑GATTCTTC‑CCCCA‑GATTGCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCATCTGCCTTCGCCTGGGCt                                          <  1:129906/235‑1 (MQ=255)
                                                                                                                                                                                                                              cGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTAACCTCCGCCC‑GATTCTTT‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagct                                    >  2:374566/1‑249 (MQ=255)
                                                                                                                                                                                                                                gtgttttcaccgcccccAGGCGGGCG‑ATACCCTCCGCCC‑GTTTCTTC‑CCCCA‑GATCACGGG‑AGCCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTACGCCTTGGCTTCagctagc                                 <  1:450434/237‑1 (MQ=255)
                                                                                                                                                                                                                                     tAACC‑GTACCCAGGCGGGCA‑GTAACCTCCGCCC‑GATTCTTT‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATg                           >  1:237397/1‑251 (MQ=255)
                                                                                                                                                                                                                                     tAACC‑GTACCCAGGCGGGCA‑GTAACCTCCGCCC‑GATTCTTT‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGAACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑CTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑G‑CACTTTTTTGCCGCCCCATATCCGCCGAAAAGTCGATCTGAGGACCATCCTGAGTGGCAACTACCTTCGCCTCGGCTTCAGCTAGCTGAATg                           >  1:604565/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      ggCA‑GTAACCTCCGCCC‑GATTCTTT‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑GAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAACCGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCGAccc           >  2:323113/1‑250 (MQ=255)
                                                                                                                                                                                                                                                             aaCCTCCGCCC‑GATTCTTT‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCatat     >  2:81516/1‑251 (MQ=255)
                                                                                                                                                                                                                                                                cTCCGCCC‑GATTCTTT‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGACTGCGCTGTTCAGCCACCTGCATATCCg  >  2:274330/1‑251 (MQ=255)
                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                   
GCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAA‑CGCCGCCTGGGTTTG‑CCACTCCC‑AGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTG‑CTCGC‑G‑TTCC‑GCCGCCCGTGCTTTAACC‑GTACCCAGGCGGGCA‑GTAACCTCCGCCC‑GATTCTTT‑CCCCA‑GATATCGAG‑ATGCCAGCCCGCCGTTAAGCC‑AAAAGTACC‑GTTGGTGTA‑CCACGGGCCGGTCGTACCTGCGGCCGGATCG‑TTCAGAGCAAACGGCCCCATTAAGCCTTCTGCC‑GACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCG  >  NC_000913/2228027‑2228524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑