Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	213,341	0	A	G	27.4%	141.5 / 13.0	62	G337G (GGA→GGG)	tilS	tRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (3/42);  new base G (17/0);  total (20/42)
Fisher's exact test for biased strand distribution p-value = 1.54e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTG  >  NC_000913/213093‑213588                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                          gcggcgttattCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCGGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCGATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTTTGCCGGGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAg                                                                                                                                                                                                                                                         <  2:247653/241‑1 (MQ=255)   ggcgagttgccgccgCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCGGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCGGTTTACGTTTGGGCGCGTTTGCAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAGCCGCTGGAATTACCGGCGGGGCTGGGAAgtg                                                                                                                                                                                                                                                       <  2:227214/242‑1 (MQ=255)       aTTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACa                                                                                                                                                                                                                                                  <  1:616166/251‑1 (MQ=255)         tATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGTGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAg                                                                                                                                                                                                                                                 <  2:603992/250‑1 (MQ=255)            ccgccgCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTa                                                                                                                                                                                                                                             <  1:334680/251‑1 (MQ=255)              gccgcTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGT‑AAAGCTTaa                                                                                                                                                                                                                                            >  1:152513/1‑250 (MQ=255)                       tggcAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGG‑GCAGCTTAAAGCggggggg                                                                                                                                                                                                                                  >  1:329587/1‑247 (MQ=255)                        ggcagggcagAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAgg                                                                                                                                                                                                                                   <  1:167016/249‑1 (MQ=255)                             ggcagAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCGGGGGAATGG‑GCAGCTTAATGAggggggggggaat                                                                                                                                                                                                                            >  1:291901/1‑241 (MQ=255)                                agAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGG‑AAAGCTTAATGCGGGGGGCGAGAttagc                                                                                                                                                                                                                         >  1:529899/1‑248 (MQ=255)                                  aaTGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGT‑ACAGCCTAATGCGGGGGGCGATATTTGccc                                                                                                                                                                                                                       >  1:182183/1‑251 (MQ=255)                                   aTGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGG‑TCAGCTTTATGCGGGGGGGGAGATTTGCCCt                                                                                                                                                                                                                      >  1:448596/1‑251 (MQ=255)                                     gCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTcc                                                                                                                                                                                                                    <  2:586410/251‑1 (MQ=255)                                         cGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGG‑ACAGGGTTATGCCGGAGGCggtctttctcctccgcgc                                                                                                                                                                                                                >  1:32354/1‑234 (MQ=255)                                          gATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTg                                                                                                                                                                                                              <  1:99676/251‑1 (MQ=255)                                                 tCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACg                                                                                                                                                                                                        <  1:8898/250‑1 (MQ=255)                                                        aCGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGT‑AACGCTTAATTCGGGAGGCGGTATTACCCCTCCGC‑GGGCAGACGGAGCGGTc                                                                                                                                                                                                >  2:511787/1‑251 (MQ=255)                                                                    ggATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTc                                                                                                                                                                                    <  1:85706/251‑1 (MQ=255)                                                                          ggCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGT‑GCAGCTTAATGCGGGGGGCGGGATTTGCCCTCCGC‑GTGCCGACGAAGCGGGCAgcgtgcggttttaagcg                                                                                                                                                                              >  1:222065/1‑241 (MQ=255)                                                                          ggCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAg                                                                                                                                                                                <  1:332469/249‑1 (MQ=255)                                                                           gCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGT‑ACAGCTTAATGCGGGAGGCGGTAATTGCCCCCCCC‑GTGCAGACGAAGCGGGCAGCGGGCGTTTTAAAacgc                                                                                                                                                                             >  1:218698/1‑251 (MQ=255)                                                                              ggAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGT‑ACAGCTTAATGCGGGGGGGGGTATTTGCCCTCCGC‑GTGCCGACGAAACGGGCAGGGTGCGGTTCAAAGCGcccg                                                                                                                                                                          >  1:97955/1‑249 (MQ=255)                                                                                 aGTGGCGCTGGCGCGGGAAGATGCCTCCCCCTGTTTACGGTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGGTTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGAt                                                                                                                                                                       <  2:593827/251‑1 (MQ=255)                                                                                 aGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGAt                                                                                                                                                                       <  2:6285/251‑1 (MQ=255)                                                                                  gTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGAtt                                                                                                                                                                      <  2:532854/251‑1 (MQ=255)                                                                                       gctggcgcGGGAAGATGCCTCTCCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATtgctgc                                                                                                                                                                 <  2:451029/251‑1 (MQ=255)                                                                                         tggcgcGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTTAC‑GCTTAATTCGGGGGGCGATATTCTCCCCCCGCCGTG‑AGACCAAGCGGTCAGCGGGCGGTTCaaaacccccgggtttgtgcat                                                                                                                                                               >  2:321525/1‑233 (MQ=255)                                                                                                 gAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGT‑ACAGCTTAATTCGGGAGGGGGGATTTGCCCTCCGC‑GTGCCGAAGAAGGGGGCAGCGGGCGTTTCAAAACGCCAGGGTTGTTGCGTAttttctg                                                                                                                                                       >  1:369783/1‑246 (MQ=255)                                                                                                       gccgcACCCTGGTTCCGTTGGGGCGCGTTTGAAAGCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTaa                                                                                                                                                 <  2:222065/247‑1 (MQ=255)                                                                                                       gCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTa                                                                                                                                                  <  1:402953/250‑1 (MQ=255)                                                                                                        ccgcACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCGTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAc                                                                                                                                                <  2:78799/248‑1 (MQ=255)                                                                                                                 gTTTACGGTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGt                                                                                                                                       <  2:204938/251‑1 (MQ=255)                                                                                                                   ttACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTa                                                                                                                                      <  1:276147/250‑1 (MQ=255)                                                                                                                    tACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTaa                                                                                                                                     <  1:557792/250‑1 (MQ=255)                                                                                                                           gggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAg                                                                                                                              <  1:605600/250‑1 (MQ=255)                                                                                                                                            gACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGG‑ACAGCTTAATGCGGGAGGCGGTATTCGCCCCCCCC‑GTGCAGACGAAGCGGTCAGCGGGCGGTTCTAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGGCGTAAGCTAAAGAAAATCTTGGAAGAGCTg                                                                                                            >  1:41340/1‑251 (MQ=255)                                                                                                                                               gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGc                                                                                                         <  1:321525/251‑1 (MQ=255)                                                                                                                                                  aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGT‑ACAACTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGGCGACGAAGCGGGCAGCGTGCGGTTCCAAGCGCCCGGATTGCTGCATATTGTCGGGCGGAAAGGCGGGCGGAAACTAAAAAAAAACTGGCAAGAGCTggggggg                                                                                                      >  1:28862/1‑247 (MQ=255)                                                                                                                                                        cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGGAAACATTGTGCCGTGGCAGACGTGGCTTCACCCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGccgccg                                                                                                <  2:227486/251‑1 (MQ=255)                                                                                                                                                         ccAACCGTGGGGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGt                                                                                               <  1:504132/250‑1 (MQ=255)                                                                                                                                                            aCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGc                                                                                            <  1:492997/251‑1 (MQ=255)                                                                                                                                                               gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTa                                                                                          <  2:208340/250‑1 (MQ=255)                                                                                                                                                               gtggtgGACGAAATCCGTCACCGGGCTAAGCGGAAACATTGTGCCGTGTCAGACTTGTCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATAGTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTACCGGCGGTCGTAAGCTAAAGAAAATCTGGCAATAGCTGGGCTTGCCGCCGTGGCTAc                                                                                         <  2:23150/251‑1 (MQ=255)                                                                                                                                                                       ttAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGT‑AAAGCTCAATGCGGGGGGGGGTATTCGCCCTCCGC‑GTGCCGACGAAACGGGCAGCGTGCGGTTCAAAGCGCCCGGATTGCTGCATATTGTCGGGCGTAAAGGCGGGCGTAAAGTAAAAAAAAACTGGCAAAAGGTGGGGGGGGCGCCGCGGGTACGGGAcacc                                                                                 >  1:605451/1‑251 (MQ=255)                                                                                                                                                                          aaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGT‑ACCGCTTTATGCGGGAGGCGGTATTCGCCCTCCGC‑GGGCAGACGAAGCGGGCAGCGGGCGGTTCAAAGCGCCAGGGTTGCTGCCTATTGTCGGGCGTAAAGGCGGGCGGAAGCTAAAAAAAATCTGGCAAGAGAGGGGGGGGGCGCCGGGGCTACGTGAgacccac                                                                              >  1:557688/1‑248 (MQ=255)                                                                                                                                                                               gtccccGGGCAAAGCGAAAACTTTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCAct                                                                         <  2:97955/247‑1 (MQ=255)                                                                                                                                                                                       gCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGtttt                                                                 <  1:271529/251‑1 (MQ=255)                                                                                                                                                                                         aaaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTAt                                                               <  1:148364/251‑1 (MQ=255)                                                                                                                                                                                              gAAAACATTGTGCCGTGGCAGACTTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGa                                                          <  1:589309/251‑1 (MQ=255)                                                                                                                                                                                               aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGaa                                                         <  1:526307/251‑1 (MQ=255)                                                                                                                                                                                                   cATTGTGCCGTGGCAGAAGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCGTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGTGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGc                                                     <  2:536593/251‑1 (MQ=255)                                                                                                                                                                                                       gtgcggttgCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGTAAGTGT‑ACAGCTTAATGCGGGAGGCGAGATTCGCGCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATAGTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGAt                                                 <  2:49809/243‑1 (MQ=255)                                                                                                                                                                                                                     gTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAgggg                                   <  2:448596/251‑1 (MQ=255)                                                                                                                                                                                                                       ggCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTa                                 <  1:107737/251‑1 (MQ=255)                                                                                                                                                                                                                              aCCGCTGGAATTACCGGCGGGGCGGGGAAGTGT‑ACGGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGGCGAAGCGGTCAGCGTGCGTGTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGGGTGCCGCCGTGGCTACGTGACACCACGCCAGTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGa                          <  2:317590/251‑1 (MQ=255)                                                                                                                                                                                                                                    ggAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAATCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGTACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCa                      <  2:505657/249‑1 (MQ=255)                                                                                                                                                                                                                                    ggAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCa                      <  2:329587/249‑1 (MQ=255)                                                                                                                                                                                                                                             ggcggGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTgg           <  2:527828/251‑1 (MQ=255)                                                                                                                                                                                                                                               cggGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTTCGGCGGGCGGGGGATTtgtggccgaggaagggtgtggc         >  2:157719/1‑233 (MQ=255)                                                                                                                                                                                                                                                      gggAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGGCACCACGCCCCTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCGGGGGGCTTTGTGAAGCAAGAAgggtggggtgaagggg  >  2:524513/1‑237 (MQ=255)                                                                                                                                                                                                                                                      gggAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGt   <  2:41340/250‑1 (MQ=255)                                                                                                                                                                                                                                                       ggAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCGAGAAGGTGTGGCTGAAGGTg  >  1:552740/1‑250 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                          GCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGT‑ACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGC‑GTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTG  >  NC_000913/213093‑213588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑