| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,096,090 | 0 | A | G | 34.4% | 125.8 / 11.8 | 64 | I83V (ATT→GTT) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base A (6/36); new base G (22/0); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.69e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAA‑CCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTC > NC_000913/2095851‑2096323 | cAAAACGCATAATACCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGTTATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGAGCTGACGATCTGGGGTTCTACGAAATCACCGCGTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTAGG‑agttaaactctg > 1:227041/1‑240 (MQ=255) aaaaCGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑TTAAGTCTTT‑gg > 1:98971/1‑251 (MQ=255) aaaCGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGa < 2:309969/251‑1 (MQ=255) aCGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑A‑TTAAATCTCT‑ggg > 1:576837/1‑248 (MQ=255) cGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑GTAAGTCTCT‑GGa > 1:553725/1‑248 (MQ=255) gCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGAt < 1:46596/251‑1 (MQ=255) aTAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑G‑TTaaaatttttgaggatgc > 1:142421/1‑236 (MQ=255) aaTCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGcc < 1:71986/250‑1 (MQ=255) cccATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑G‑TTaggagtcaggcaaatgcagcgg > 2:40816/1‑230 (MQ=255) cccATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCcgc‑g < 1:124864/250‑1 (MQ=255) aTGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑aaaa < 2:121604/251‑1 (MQ=255) cGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCttt < 1:296515/251‑1 (MQ=255) ttCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑GTAAGACTCT‑GGGAGAGGTCGC‑G‑GAAAATCTTTccctt > 2:620630/1‑248 (MQ=255) cGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTgg < 1:577919/251‑1 (MQ=255) cGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCGACGCTGAAGAAGGCGCGGACGAACTGGTGTTCTACGATATAACCGCTACCGGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑CTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTgg < 1:473177/251‑1 (MQ=255) gTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAAGCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGgc < 2:258984/251‑1 (MQ=255) tGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑G‑ATAAGGTTCT‑GGGAGATGGCTC‑GGAAAATTTTTTCTTTTggg > 2:636700/1‑250 (MQ=255) cAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑G‑TTAAGTCTCT‑GGGAGAAGCCCCCG‑AAAAT‑CTTTCCTTTTGCGCGG‑ATa > 1:395976/1‑250 (MQ=255) tggtgAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑TTAAAGCTCT‑TGAAGATTCCCC‑G‑AAAATTTTTTTCttttgggccggtaaaaat > 1:562108/1‑236 (MQ=255) ggtgAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑TTAAGGCTCT‑GGAAGATGCCCC‑G‑AAAATTCTTTTCTTTTGCGCGG‑GTAAAAttt > 1:582331/1‑251 (MQ=255) gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTccc > 1:116223/1‑250 (MQ=255) aaGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑GGTTAAG‑CTCT‑TGGAGATGGCGC‑G‑GAAATTCTTTTCTTTTGGGCGGGAAAAAATTTcatt > 2:434829/1‑248 (MQ=255) aaGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑TTTATGCTCT‑GTGAGGTGCCCC‑G‑AAAAATCTTTCCTTTTTGGCGG‑GTAAAATTTTCATc > 2:165657/1‑251 (MQ=255) ggCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAAtt < 1:276566/243‑1 (MQ=255) ggCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑TTAAGTCTTT‑GGGAGATGGCGC‑GGAAAATTTTTTCTTTTgggggggtaaaaatataacggg > 2:276566/1‑231 (MQ=255) gTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑G‑TTAAGTCTCT‑GGGAGATGCGGC‑G‑AAAATTCTTTTCTTTGGGGCGG‑ATAAAATTTTCATCAActc > 1:319895/1‑251 (MQ=255) cAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTcc < 1:639391/251‑1 (MQ=255) aGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCt < 1:620630/251‑1 (MQ=255) aGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑TTAAGTCTCT‑TGAAGAAGCCGC‑G‑AAAATTCTTTCCTTTTGCGCGG‑ATAAAATTTCCATCAACTCTccc > 1:181679/1‑250 (MQ=255) gTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑TTAAATCTCTTGGAAG‑TGCCGC‑G‑AAAAATCTTTCCTTTTGCGCGG‑GAAAAAATTCCATCaaacaccctc > 1:550312/1‑243 (MQ=255) ccATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑TTTAGTCTCT‑GGAAAATACACC‑G‑AAAATTATTTCCCTTTTCGGGG‑GTAAAATTTCCACCAACTCTCCTGCGCTGGc > 2:487537/1‑249 (MQ=255) gCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACattaatt < 1:579954/251‑1 (MQ=255) gATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTAc < 1:540368/251‑1 (MQ=255) atatCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGA‑TAAAG‑CTCT‑GGAAGATGCCGC‑G‑AAAAATCTTTTCTTTTGCCCGG‑GTATAATTTCCCTCAACTATCCTGCGTTGGCCGACCCCACATTAATTAcc > 1:327492/1‑250 (MQ=255) tatCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑GTAAGTCTCT‑GGAAGAAGCCGC‑G‑AAAATTCTTTCCTTTTGGGCGG‑GTAAAATTTCCCACAACTCTTCTGCCCCGGGAGACCCAACACTAAATACTc > 1:487105/1‑251 (MQ=255) atCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCGCCTGCGCTGGCAGACCCAACATTAATTACTc < 1:35342/250‑1 (MQ=255) gTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑GAAATTCTTTCCTTTTGCGCGG‑ATGAAAATTCCCTCAACTCTCCCGCGCCGGCAGACCCAACATTTATTACttcccc > 1:248553/1‑246 (MQ=255) ggCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCTCCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATc < 1:108293/251‑1 (MQ=255) ggCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCGCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGAt < 2:134823/250‑1 (MQ=255) cAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGc < 1:389834/251‑1 (MQ=255) acgctacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTg < 1:355436/251‑1 (MQ=255) gctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑TTTAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTTGCGCGG‑GTAAAATTTTCACCACCTCTCCTGCGCTGGGACACCCCAAAATAATTACTCCCCTGGcccctcccttttgcgtggg > 2:479874/1‑234 (MQ=255) ggCGCGGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATtgt < 2:395976/251‑1 (MQ=255) ggcGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCATCTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTc < 1:437734/247‑1 (MQ=255) gACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCgg < 2:562108/251‑1 (MQ=255) gtgtTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGAGAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGTCCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATAc < 1:195655/251‑1 (MQ=255) tACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑A‑ATTAGTCTCT‑GGAAGATGCCCC‑G‑CAAATTCTTTCCTTTGGCGCGG‑ATAAAAATTCCCTCAACCCTCCTGCGCTGGCAGACCCAACATTAATTACCCGCCCGGCCGATCGCTTTGGCGCGCAGGGTATTTTGGTCGGTATTGATACCttgg > 1:167227/1‑247 (MQ=255) aCGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAAGTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTgg < 2:98971/248‑1 (MQ=255) aCGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑TTAAGTCTCT‑GGGAGATGCCGC‑G‑AAAATTCTTTCCTTTTGGGCGG‑GTTAAAATTCCATCCACTCTCCTGCGCTGGCCGACCCAACATTAATTACTCGCCCGGCCGATCGCTTTGGCGGGCCGGGTATTTTTGTCGGTATTTATACCCGGTac > 1:527729/1‑251 (MQ=255) cGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTacg < 1:440863/251‑1 (MQ=255) atatCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTacgac < 1:360132/251‑1 (MQ=255) atatCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTacgac < 1:92521/251‑1 (MQ=255) atatCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTacgac < 2:116223/251‑1 (MQ=255) catcGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAA‑CCGGTa < 2:130382/248‑1 (MQ=255) cGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGG‑G‑TTAAGACTCT‑GGAAGAAGCCGC‑G‑AAAAATTTTTTCTTTTGCGCGG‑GTAAAATTTCCATCAACCCTCCTTCGCTGGCAGAACCCACATTAATTTATCCCCTGGCCCATCCCTTTGGCGTGCAGGGGATTGTGGGTGGTCTTGATACCTCGTACGCCGCCGTAAC‑CCGGTaat > 2:111655/1‑250 (MQ=255) gCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAA‑CCGGTAAATATCAt < 2:628493/251‑1 (MQ=255) cgcgTGGCGGAAGTGATCGACATTCCGTTTTGTGTTGCGGGTGGG‑A‑TTAAGTTTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTGCCAGCAACTCTCCTGCGCTGGCAGACCCAACACTAATTACTCGCCTGGCCTATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAA‑CCGGTAAATATCATGTTAATCAATATACCGTCGATGAAAGcct < 2:227041/251‑2 (MQ=255) cgcgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAA‑CCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGcc < 2:571945/250‑1 (MQ=255) gCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTGGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAA‑CCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCcg < 2:576837/251‑1 (MQ=255) gATCGACATTCCGTTTTGTGTGGCGGGTGGG‑G‑TTTAGTCTCT‑TGAAGATGCCGC‑G‑AAAATTCTTTTCTTTTGCGCGG‑GTGAAATTTTCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTTGCGTGCAGGGGATTGTGGTCGGGATTGATAACTGGTACGACGCCG‑GAA‑CCGGGAAAAATCATTTGAATCAATATAACGGCGATGAAAACCGCACCCGCGTCACTc > 1:536902/1‑251 (MQ=255) gATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCCC‑G‑AAAATTTTTTCCTTTGGCGCGG‑GTAAAATTTCCCACAACTCCCCTGCGCTGGCAGACCCCACAATAATTAATCGCCCGGCCGATCGCCTTGGCGGGCAGTGGATTGTGGTCGGGATTGATACCTGGTAAGACGCCG‑AAAACGGGTAAA‑ATCATGTGAATCAATATACCGGCGCGGAAAACCGCCACCGCGTCCCTc > 2:483610/1‑251 (MQ=255) ccGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAA‑CCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAAc < 2:582331/251‑1 (MQ=255) gtgtgtgGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAA‑CCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTc < 2:487105/249‑1 (MQ=255) tGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAA‑CCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCAc < 1:391002/213‑1 (MQ=255) tGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAA‑CCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCAc > 2:391002/1‑213 (MQ=255) | CAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGG‑A‑TTAAGTCTCT‑GGAAGATGCCGC‑G‑AAAATTCTTTCCTTTGGCGCGG‑ATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAA‑CCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTC > NC_000913/2095851‑2096323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |