breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 38,145 G→A T324I (ACC→ATC)  caiB ← gamma‑butyrobetainyl‑CoA:carnitine CoA transferase
RA 38,163 G→A A318V (GCC→GTC)  caiB ← gamma‑butyrobetainyl‑CoA:carnitine CoA transferase
RA 42,108 G→A intergenic (‑177/‑295) caiT ← / → fixA L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA
RA 75,070 C→T P137P (CCG→CCA thiB ← thiamine ABC transporter periplasmic binding protein
RA 81,498 C→T G154D (GGT→GAT)  leuB ← 3‑isopropylmalate dehydrogenase
RA 226,027 C→T noncoding (269/2904 nt) rrlH → 23S ribosomal RNA
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 260,015 C→T G29D (GGT→GAT)  phoE ← outer membrane porin PhoE
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 299,256 G→A A560V (GCC→GTC)  paoC ← aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
RA 300,585 G→A A117V (GCC→GTC)  paoC ← aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
RA 300,645 G→A A97V (GCC→GTC)  paoC ← aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
RA 303,911 C→T G91E (GGA→GAA)  ykgJ ← putative metal‑chelating domain‑containing protein YkgJ
RA 305,596 G→A L408F (CTC→TTC)  ecpD ← fimbrial adhesin EcpD
RA 306,474 C→T G115D (GGC→GAC)  ecpD ← fimbrial adhesin EcpD
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
RA 337,418 G→A G214D (GGC→GAC)  yahF → putative acyl‑CoA synthetase YahF
RA 342,413 A→G D430G (GAC→GGC)  yahJ → amidohydrolase family protein YahJ
RA 363,362 C→T G982S (GGC→AGC)  lacZ ← beta‑galactosidase
MC JC 402,391 Δ723 bp coding (645‑1367/1416 nt) phoA → alkaline phosphatase
RA 426,428 C→T L98F (CTT→TTT)  tgt → tRNA‑guanine transglycosylase
RA 429,132 C→T L496L (CTG→TTG)  secD → Sec translocon accessory complex subunit SecD
RA 435,272 C→T L46L (CTG→TTG)  nusB → transcription antitermination protein NusB
RA 446,642 C→T G9S (GGT→AGT)  yajR ← putative transport protein YajR
RA 456,645 C→T intergenic (+214/‑32) tig → / → clpP trigger factor/ATP‑dependent Clp protease proteolytic subunit
RA 458,220 C→T G265G (GGC→GGT clpX → ATP‑dependent Clp protease ATP‑binding subunit ClpX
RA 460,036 C→T G383G (GGC→GGT lon → Lon protease
RA 466,776 C→T G179G (GGG→GGA ybaE ← uncharacterized protein YbaE
RA 472,585 C→T intergenic (+168/‑13) mdlB → / → glnK ABC transporter family protein MdlB/nitrogen regulatory protein PII‑2
RA 474,379 C→T V261V (GTG→GTA tesB ← acyl‑CoA thioesterase II
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
RA 480,686 C→T R8K (AGA→AAA)  tomB ← Hha toxicity modulator TomB
RA 487,802 C→T L423L (CTG→TTG)  mscK → potassium dependent, small conductance mechanosensitive channel
RA 492,215 C→T L42F (CTT→TTT) 
L42F (CTT→TTT) 
dnaX →
dnaX →
DNA polymerase III subunit tau
DNA polymerase III subunit tau
RA 496,241 C→T F374F (TTC→TTT htpG → chaperone protein HtpG
RA 500,069 C→T intergenic (‑96/‑56) aes ← / → gsk acetylesterase/inosine/guanosine kinase
RA 500,641 C→T Q173* (CAG→TAG)  gsk → inosine/guanosine kinase
RA 501,193 G→A G357R (GGG→AGG)  gsk → inosine/guanosine kinase
RA 508,652 C→T intergenic (+93/+223) ybaQ → / ← copA DNA‑binding transcriptional regulator YbaQ/Cu(+) exporting P‑type ATPase
RA 511,602 G→A intergenic (‑223/‑39) copA ← / → glsA Cu(+) exporting P‑type ATPase/glutaminase 1
RA 512,904 C→T A110V (GCC→GTC)  ybaT → putative transporter YbaT
RA 518,704 C→T G149E (GGA→GAA)  ybbO ← NADP(+)‑dependent aldehyde reductase YbbO
RA 518,989 C→T R54H (CGC→CAC)  ybbO ← NADP(+)‑dependent aldehyde reductase YbbO
RA 519,280 C→T L162L (CTG→CTA tesA ← multifunctional acyl‑CoA thioesterase I and protease I and lysophospholipase L1
RA 606,604 G→A T260T (ACC→ACT ybdK ← putative glutamate‑‑cysteine ligase 2
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
RA 789,597 C→T S128N (AGT→AAT)  galK ← galactokinase
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 891,983 T→A L6* (TTG→TAG)  rimK → ribosomal protein S6 modification protein
RA 908,760 A→T C179S (TGC→AGC)  ltaE ← low‑specificity L‑threonine aldolase
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 1,005,503 A→G N246D (AAT→GAT)  pyrD → dihydroorotate dehydrogenase, type 2
RA 1,040,126 G→T L277L (CTG→CTT appB → cytochrome bd‑II subunit 2
RA 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,115,233 C→T M86I (ATG→ATA mdtG ← efflux pump MdtG
RA 1,129,324 C→T L496L (CTG→TTG)  murJ → lipid II flippase MurJ
RA 1,133,668 C→T V365V (GTC→GTT flgE → flagellar hook protein FlgE
RA 1,155,389 C→T A89V (GCT→GTT)  tmk → Tmk
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,180,426 T→A intergenic (+67/+53) cobB → / ← ycfZ protein‑lysine deacetylase/desuccinylase/lipoamidase/putative inner membrane protein YcfZ
RA 1,181,285 C→T R147R (AGG→AGA ymfA ← putative inner membrane protein YmfA
RA 1,186,294 G→A A151T (GCG→ACG)  pepT → peptidase T
RA 1,189,296 C→T V161I (GTA→ATA)  phoQ ← sensor histidine kinase PhoQ
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,278,991 C→T G345G (GGC→GGT narK → nitrate:nitrite antiporter NarK
JC JC 1,293,268 IS5 (–) +4 bp intergenic (‑346/‑256) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
RA 1,301,998 A→G S273G (AGC→GGC)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,302,268 C→T Q363* (CAG→TAG)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,397,802 G→A T274I (ACC→ATC)  uspE ← universal stress protein E
JC JC 1,431,062 IS2 (–) +5 bp coding (2014‑2018/3363 nt) stfR → putative prophage side tail fiber protein StfR
RA 1,479,799 C→T P221L (CCC→CTC)  ynbC → hydrolase/methyltransferase domain‑containing protein YnbC
RA 1,553,310 C→T A177T (GCC→ACC)  adhP ← ethanol dehydrogenase/alcohol dehydrogenase
RA 1,600,234 C→T intergenic (‑25/+54) lsrK ← / ← lsrR autoinducer‑2 kinase/DNA‑binding transcriptional repressor LsrR
RA 1,601,098 C→A S48S (TCG→TCT lsrR ← DNA‑binding transcriptional repressor LsrR
RA 1,601,103 C→T V47M (GTG→ATG)  lsrR ← DNA‑binding transcriptional repressor LsrR
RA 1,632,712 A→G intergenic (‑427/‑178) ydfJ ← / → ynfT putative transporter YdfJ/protein YnfT
RA 1,632,735 G→A intergenic (‑450/‑155) ydfJ ← / → ynfT putative transporter YdfJ/protein YnfT
RA 1,641,002 T→C intergenic (+27/+337) ynfU → / ← cspB putative zinc‑binding protein YnfU/cold shock‑like protein CspB
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,649,971 C→T P60S (CCG→TCG)  ydfD → lysis protein
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,702,757 C→T F175F (TTC→TTT add → adenosine deaminase
MC JC 1,757,529 Δ63 bp coding (109‑171/237 nt) lpp → murein lipoprotein
RA 1,793,377 Δ1 bp coding (182/750 nt) btuD ← vitamin B12 ABC transporter ATP binding subunit
RA 1,861,311 C→T G8S (GGC→AGC)  ydjL ← putative zinc‑binding dehydrogenase YdjL
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,964,254 A→C V266G (GTC→GGC)  flhA ← flagellar biosynthesis protein FlhA
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
JC 2,092,996 (TGGC)4→3 coding (599‑602/1071 nt) hisC → histidinol‑phosphate aminotransferase
RA 2,099,525 A→T I30N (ATT→AAT)  ugd ← UDP‑glucose 6‑dehydrogenase
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,155,880 C→T L206L (CTC→CTT mdtB → multidrug efflux pump RND permease subunit MdtB
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,173,448 C→T intergenic (‑575/+834) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,224,169 A→G F234F (TTT→TTC pbpG ← peptidoglycan DD‑endopeptidase PbpG
RA 2,237,105 G→T P217T (CCG→ACG)  mglC ← D‑galactose/methyl‑galactoside ABC transporter membrane subunit
JC JC 2,237,684 IS5 (+) +4 bp coding (67‑70/1011 nt) mglC ← D‑galactose/methyl‑galactoside ABC transporter membrane subunit
RA 2,264,460 2 bp→AT coding (598‑599/1182 nt) setB → sugar exporter SetB
RA 2,319,078 C→A D267Y (GAT→TAT)  rcsC ← histidine kinase RcsC
RA 2,360,550 Δ1 bp coding (865/1206 nt) rhmD ← L‑rhamnonate dehydratase
RA 2,392,233 C→G G270A (GGT→GCT)  nuoM ← NADH:quinone oxidoreductase subunit M
RA 2,423,891 C→T V207I (GTA→ATA)  hisP ← lysine/arginine/ornithine ABC transporter/histidine ABC transporter, ATP binding subunit
RA 2,443,447 G→A A562T (GCG→ACG)  mnmC → fused tRNA 5‑aminomethyl‑2‑thiouridylate methyltransferase and tRNA 5‑aminomethyl‑2‑thiouridylate synthase
RA 2,473,392 G→A intergenic (‑146/‑212) yfdO ← / → yfdP protein YdfO/uncharacterized protein YfdP
RA 2,473,397 +T intergenic (‑151/‑207) yfdO ← / → yfdP protein YdfO/uncharacterized protein YfdP
RA 2,510,609 C→T P327L (CCG→CTG)  yfeO → putative transport protein YfeO
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
RA 2,630,852 G→A S176N (AGC→AAC)  yfgI → nalidixic acid resistance protein YfgI
RA 2,732,992 G→A I394I (ATC→ATT
I394I (ATC→ATT
clpB ←
clpB ←
chaperone protein ClpB
chaperone protein ClpB
RA 2,745,565 G→T T118T (ACC→ACA rimM ← ribosome maturation factor RimM
RA 2,804,133 C→T S211F (TCT→TTT)  nrdF → ribonucleoside‑diphosphate reductase 2 subunit beta
RA 2,814,459 Δ1 bp coding (275/516 nt) luxS ← S‑ribosylhomocysteine lyase
RA 2,814,461 C→T L91L (CTG→CTA luxS ← S‑ribosylhomocysteine lyase
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,871,153 T→G N51T (AAC→ACC)  truD ← tRNA pseudouridine(13) synthase
JC JC 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase
RA 2,920,153 G→A Y198Y (TAC→TAT gudX ← glucarate dehydratase‑related protein
RA 2,954,158 G→A L616L (CTG→TTG)  recB ← exodeoxyribonuclease V subunit RecB
RA 2,955,385 C→T V207I (GTT→ATT)  recB ← exodeoxyribonuclease V subunit RecB
RA 3,010,122 G→A G32D (GGC→GAC)  hyuA → phenylhydantoinase
RA 3,019,751 C→T I197I (ATC→ATT ssnA → putative aminohydrolase SsnA
RA 3,063,827 C→T Q281* (CAG→TAG)  argK → methylmalonyl‑CoA mutase‑interacting GTPase YgfD
RA 3,076,531 G→A T309M (ACG→ATG)  yggP ← putative zinc‑binding dehydrogenase YggP
RA 3,127,435 G→T S196* (TCG→TAG)  glcD ← glycolate dehydrogenase, putative FAD‑linked subunit
RA 3,175,234 C→T V55M (GTG→ATG)  parE ← DNA topoisomerase IV subunit B
RA 3,208,066 G→A A15T (GCG→ACG)  ttdT → L‑tartrate:succinate antiporter
RA 3,220,571 G→A E360K (GAG→AAG)  patA → putrescine aminotransferase
RA 3,221,786 C→T S107S (AGC→AGT ebgR → DNA‑binding transcriptional repressor EbgR
RA 3,230,486 C→T D533D (GAC→GAT ygjK → glycoside hydrolase
RA 3,243,050 G→A Q89* (CAG→TAG)  uxaA ← D‑altronate dehydratase
MC JC 3,258,933 (ACGGC)2→1 coding (713‑717/1365 nt) tdcG ← L‑serine deaminase III
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
MC JC 3,349,206 Δ16,350 bp IS5‑mediated [mtgA][yhcE] [mtgA], elbB, arcZ, arcB, yhcC, gltB, gltD, gltF, yhcA, yhcD, [yhcE]
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,432,105 C→T L120L (CTG→CTA smg ← DUF494 domain‑containing protein Smg
RA 3,443,961 G→A A46V (GCC→GTC)  secY ← Sec translocon subunit SecY
RA 3,443,980 G→A P40S (CCG→TCG)  secY ← Sec translocon subunit SecY
RA 3,452,022 G→A P89S (CCG→TCG)  rplD ← 50S ribosomal subunit protein L4
RA 3,452,218 G→A F23F (TTC→TTT rplD ← 50S ribosomal subunit protein L4
RA 3,457,378 C→T L334L (CTC→CTT gspD → Type II secretion system protein GspD
RA 3,458,245 A→C S623S (TCA→TCC gspD → Type II secretion system protein GspD
RA 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,492,293 A→G F2S (TTC→TCC)  ppiA ← peptidyl‑prolyl cis‑trans isomerase A
RA 3,506,393 T→C D315G (GAC→GGC)  yhfT ← uncharacterized protein YhfT
RA 3,560,455 1 bp→TG intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,562,125 C→T A38V (GCT→GTT)  glpD → aerobic glycerol 3‑phosphate dehydrogenase
RA 3,563,255 C→T R415C (CGT→TGT)  glpD → aerobic glycerol 3‑phosphate dehydrogenase
RA 3,586,657 G→A A56V (GCC→GTC)  ggt ← glutathione hydrolase proenzyme
RA 3,626,067 C→T G246D (GGT→GAT)  yhhJ ← ABC transporter family protein YhhJ
RA 3,638,300 G→A G220D (GGC→GAC)  pitA → metal phosphate:H(+) symporter PitA
RA 3,682,515 C→T M311I (ATG→ATA dctA ← C4 dicarboxylate/orotate:H(+) symporter
RA 3,692,114 C→T S165N (AGC→AAC)  bcsB ← cellulose synthase periplasmic subunit
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,729,955 2 bp→AA coding (810‑811/1323 nt) xylA ← xylose isomerase
JC JC 3,731,420 IS1 (–) +9 bp coding (290‑298/993 nt) xylF → xylose ABC transporter periplasmic binding protein
RA 3,769,447 G→A E35K (GAA→AAA)  yibV → PF15596 family protein YibV
RA 3,773,040 (G)6→4 coding (760‑761/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,783,983 G→A S49F (TCC→TTC)  secB ← protein export chaperone SecB
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
JC 3,822,651 (CAGGAT)1→2 coding (252/2109 nt) spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,823,162 C→T H255Y (CAC→TAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,835,828 G→A F34F (TTC→TTT yicJ ← putative xyloside transporter YicJ
RA 3,837,106 G→A V52I (GTA→ATA)  setC → putative arabinose exporter
RA 3,837,362 G→A G137D (GGC→GAC)  setC → putative arabinose exporter
RA 3,889,668 C→T G313G (GGC→GGT tnaA → tryptophanase
RA 3,894,233 C→T P118L (CCC→CTC)  yieE → putative phosphopantetheinyl transferase
RA 3,896,888 C→T P39S (CCT→TCT)  yieH → 6‑phosphogluconate phosphatase
RA 3,899,297 C→T A33T (GCA→ACA)  yieK ← putative glucosamine‑6‑phosphate deaminase YieK
RA 3,907,444 G→A N45N (AAC→AAT phoU ← negative regulator of the pho regulon
JC JC 3,931,171 IS4 (–) +12 bp intergenic (‑78/‑134) ravA ← / → kup regulatory ATPase RavA/K(+):H(+) symporter Kup
RA 3,953,043 A→G D187G (GAT→GGT)  ilvE → branched‑chain‑amino‑acid aminotransferase
RA 3,993,531 T→C R793R (CGT→CGC cyaA → adenylate cyclase
RA 4,057,150 C→T A295A (GCG→GCA glnA ← glutamine synthetase
RA 4,058,473 G→A D23N (GAC→AAC)  bipA → 50S ribosomal subunit assembly factor BipA
RA 4,058,823 G→A W139* (TGG→TGA bipA → 50S ribosomal subunit assembly factor BipA
RA 4,085,516 G→A H103Y (CAC→TAC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,110,415 C→T S162S (AGC→AGT cdh → CDP‑diacylglycerol diphosphatase
RA 4,116,753 C→T R157H (CGT→CAT)  glpK ← glycerol kinase
RA 4,117,060 C→T A55T (GCC→ACC)  glpK ← glycerol kinase
RA 4,117,270 G→A P274L (CCT→CTT)  glpF ← glycerol facilitator
JC JC 4,117,984 IS30 (+) +2 bp coding (106‑107/846 nt) glpF ← glycerol facilitator
RA 4,119,767 C→T A195T (GCA→ACA)  menA ← 1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase
RA 4,179,329 G→A E151K (GAA→AAA)  rplA → 50S ribosomal subunit protein L1
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,242,914 C→T Q201Q (CAG→CAA malG ← maltose ABC transporter membrane subunit MalG
RA 4,259,611 G→A A131A (GCC→GCT zur ← DNA‑binding transcriptional repressor Zur
RA 4,270,596 G→A G120E (GGG→GAG)  yjbQ → PF01894 family protein YjbQ
RA 4,278,574 G→A G32G (GGG→GGA ghxP → guanine/hypoxanthine transporter GhxP
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,327,494 G→A L120L (CTG→CTA rdcA → regulator of diguanylate cyclase RdcA
RA 4,359,314 G→A Q240* (CAG→TAG)  cadB ← lysine:cadaverine antiporter
RA 4,392,997 G→A V357V (GTC→GTT queG ← epoxyqueuosine reductase
RA 4,393,552 G→A F172F (TTC→TTT queG ← epoxyqueuosine reductase
RA 4,396,373 C→T T103T (ACC→ACT amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,396,772 T→C F236F (TTT→TTC amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,400,119 G→A E290K (GAA→AAA)  miaA → tRNA dimethylallyltransferase
RA 4,413,535 G→A E170K (GAA→AAA)  yjfC → putative acid‑‑amine ligase YjfC
RA 4,416,177 C→T G39D (GGT→GAT)  yjfN ← protease activator YjfN
RA 4,420,399 G→A Q140Q (CAG→CAA ulaA → L‑ascorbate specific PTS enzyme IIC component
RA 4,425,688 C→A H57N (CAC→AAC)  priB → primosomal replication protein N
RA 4,425,696 C→A N59K (AAC→AAA priB → primosomal replication protein N
RA 4,436,956 2 bp→TT coding (202‑203/741 nt) cysQ → 3'(2'),5'‑bisphosphate nucleotidase
RA 4,472,406 C→T R43K (AGG→AAG)  pyrL ← pyrBI operon leader peptide
RA 4,486,636 G→A G140D (GGC→GAC)  lptF → lipopolysaccharide transport system protein LptF
RA 4,501,834 C→T intergenic (+245/+269) yjgZ → / ← insG uncharacterized protein YjgZ/IS4 family putative transposase
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,578,548 G→A Q264* (CAG→TAG) 
Q264* (CAG→TAG) 
mcrB ←
mcrB ←
5‑methylcytosine‑specific restriction enzyme subunit McrB
5‑methylcytosine‑specific restriction enzyme subunit McrB
RA 4,604,109 G→T A211A (GCG→GCT yjjQ → DNA‑binding transcriptional repressor YjjQ
RA 4,615,345 C→T G67S (GGC→AGC)  yjjW ← putative glycyl‑radical enzyme activating enzyme YjjW
RA 4,631,788 G→A Q352Q (CAG→CAA slt → soluble lytic murein transglycosylase
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 168355 168703 349 102 [0] [0] 124 fhuA ferrichrome outer membrane transporter/phage receptor
* * ÷ NC_000913 182569 182988 420 103 [1] [3] 127 cdaR DNA‑binding transcriptional activator CdaR
* * ÷ NC_000913 1196277 1211460 15184 36 [32] [33] 42 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1632754–1632847 1633399–1632956 110–646 34 [33] [29] 34 ynfT–ydfK ynfT,ydfK
* * ÷ NC_000913 1978495 1979270 776 130 [0] [0] 136 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423902–3424234 3424457–3424238 5–556 34 [33] [33] 36 rrlD 23S ribosomal RNA
* * ÷ NC_000913 3762431–3764074 3765125–3764076 3–2695 35 [33] [33] 35 rhsA rhs element protein RhsA
* * ÷ NC_000913 4166501–4168219 4171439–4168540 322–4939 35 [33] [27] 40 rrsB–[rrlB] rrsB,gltT,[rrlB]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1683540 (0.000)102 (0.930) 65/566 0.3 100% coding (871/2244 nt) fhuA ferrichrome outer membrane transporter/phage receptor
?NC_000913 = 182568 0 (0.000)coding (106/1158 nt) cdaR DNA‑binding transcriptional activator CdaR
* ? NC_000913 168704 =0 (0.000)124 (1.120) 68/572 0.2 100% coding (1221/2244 nt) fhuA ferrichrome outer membrane transporter/phage receptor
?NC_000913 182989 = 0 (0.000)coding (527/1158 nt) cdaR DNA‑binding transcriptional activator CdaR
* ? NC_000913 = 12994980 (0.000)140 (1.250) 83/580 0.1 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)129 (1.130) 75/588 0.1 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)136 (1.200) 71/588 0.2 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 = 4542690174 (1.530)49 (0.440) 33/570 1.9 24.5% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 133 (1.210)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit