Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	809,217	2 bp→AG	coding (40‑41/1290 nt)	bioA ←	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	809,217	0	C	A	98.7%	257.9 / ‑2.7	78	coding (41/1290 nt)	bioA	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (43/34);  minor base . (1/0);  total (44/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
*	NC_000913	809,218	0	A	G	98.7%	286.1 / NA	78	W14R (TGG→CGG)	bioA	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (43/34);  total (44/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCCAGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAA  >  NC_000913/808927‑809508                                                                                                                                                                                                                                                                                                      ||                                                                                                                                                                                                                                                                                                         aGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGc                                                                                                                                                                                                                                                                                                 <  1:104520/300‑1 (MQ=255) aGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAACTCAATGGCTGGCGCATGGGTGATACCGCCAAACACCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGc                                                                                                                                                                                                                                                                                                 <  2:125651/300‑1 (MQ=255)   tCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGt                                                                                                                                                                                                                                                                                               <  2:19510/300‑1 (MQ=255)      gcgAGAAAAACGCACTCCAGCGGTTGTGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATTGCTGGCGCATGGGTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCTCCGCATTAAGCTGCGGGTAATTTTAGCCGTGGATCGCCGCCCACCAAGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGt                                                                                                                                                                                                                                                                                               <  2:19319/297‑1 (MQ=255)                    cTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATc                                                                                                                                                                                                                                                                              >  1:401721/1‑300 (MQ=255)                                    gTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATc                                                                                                                                                                                                                                                              >  1:760087/1‑300 (MQ=255)                                       aTCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGAc                                                                                                                                                                                                                                                           <  1:44246/300‑1 (MQ=255)                                                 gTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGAGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCACAATCGACTT‑GTaaaaca                                                                                                                                                                                                                                                >  2:542042/1‑297 (MQ=255)                                                        gCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTTATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAACCGAATC‑GTAAACC‑AACTTGaa                                                                                                                                                                                                                                        >  2:133996/1‑300 (MQ=255)                                                           caGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGaaaa                                                                                                                                                                                                                                      >  2:650005/1‑299 (MQ=255)                                                           caGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACAC‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATTGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGaaaa                                                                                                                                                                                                                                      >  2:232568/1‑299 (MQ=255)                                                                      ctggcGCATGGGTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCACAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑t                                                                                                                                                                                                                            >  2:133843/1‑297 (MQ=255)                                                                           gcATGGGTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGt                                                                                                                                                                                                                    >  1:874882/1‑300 (MQ=255)                                                                              tGGGTGATACCGCCAAACCTCACAT‑GCGACATTGCATCAATTTGCGACTTCATCGCCACATGAAGCTGCGGTTAATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCTTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTc                                                                                                                                                                                                                   <  2:851224/298‑1 (MQ=255)                                                                               gggTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTac                                                                                                                                                                                                                >  1:171550/1‑300 (MQ=255)                                                                                ggTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGAGTGATTGTAGCCATGGATCGCCGCCCACCAGGACGACACACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCAGATAAACCGGCAG‑GGGGACGGTCATGGATGTGTATGAGTGC‑AGAATATGGCGTTGGTCAAAGGCAAGAGCGTCCGTTGTCATAATCGACTT‑GTCAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCt                                                                                                                                                                                                                  >  1:899192/1‑297 (MQ=255)                                                                                 ttGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTacac                                                                                                                                                                                                              <  1:795083/299‑1 (MQ=255)                                                                                 gTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTacac                                                                                                                                                                                                              <  1:795092/300‑1 (MQ=255)                                                                                   gATACCGCCAAACATCACAT‑GCTACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGTTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTacac                                                                                                                                                                                                              <  2:64793/298‑1 (MQ=255)                                                                                    aTACCGCCAAACATCACAT‑GCTACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTacac                                                                                                                                                                                                              <  2:553560/297‑1 (MQ=255)                                                                                       ccGCCAAACATCACATNGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCATGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTCGTAAACCNAAATTGAAAAGATTTAGGNTTTACAAGTCTacgacc                                                                                                                                                                                                            >  1:770585/1‑296 (MQ=255)                                                                                       ccGCCAAACATCACATNGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCATGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTCGTAAACCNAAATTGAAAAGATTTAGGNTTTACAAGTCTacaacc                                                                                                                                                                                                            >  1:770650/1‑297 (MQ=255)                                                                                            aaaCATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTaac                                                                                                                                                                                                     <  1:708526/298‑1 (MQ=255)                                                                                                         cGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAaca                                                                                                                                                                                          >  2:750375/1‑297 (MQ=255)                                                                                                               ggCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTAATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAAGCAAGATCGTCCGCTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTAAAAGTCTAAAACGAATTAACAACaaaaaaa                                                                                                                                                                                           >  2:520392/1‑289 (MQ=255)                                                                                                                gCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGgag                                                                                                                                                                                <  2:600212/300‑1 (MQ=255)                                                                                                                     aaTTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGTA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACATTTTGGAGAAGcc                                                                                                                                                                           <  1:344765/300‑1 (MQ=255)                                                                                                                      aTTTGCTACTTCATCTCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCATGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGTAGAAGcc                                                                                                                                                                           <  2:217354/299‑1 (MQ=255)                                                                                                                      aTTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGccc                                                                                                                                                                          >  1:504610/1‑300 (MQ=255)                                                                                                                               ttCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTc                                                                                                                                                                  <  1:817415/299‑1 (MQ=255)                                                                                                                                   tCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGTAGAAGCCCCATGGCTCACCGc                                                                                                                                                             <  1:800362/300‑1 (MQ=255)                                                                                                                                     gccgcATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGccc                                                                                                                                                           <  1:13458/300‑1 (MQ=255)                                                                                                                                        gcATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGcac                                                                                                                                                           >  2:609473/1‑295 (MQ=255)                                                                                                                                        gcATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑Ac                                                                                                                                                        >  1:442724/1‑299 (MQ=255)                                                                                                                                              aGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑Ac                                                                                                                                                <  1:809571/300‑1 (MQ=255)                                                                                                                                               gCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACa                                                                                                                                               <  1:350848/300‑1 (MQ=255)                                                                                                                                                    gggTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTc                                                                                                                                          >  1:704055/1‑300 (MQ=255)                                                                                                                                                        gATTGTAGCCGTGGAGCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCaa                                                                                                                                      <  2:468096/300‑1 (MQ=255)                                                                                                                                                          ttGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGt                                                                                                                                    >  1:103291/1‑300 (MQ=255)                                                                                                                                                          ttGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACAAGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGt                                                                                                                                    >  1:886461/1‑300 (MQ=255)                                                                                                                                                                               caccaGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACACCAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAAcc                                                                                                               >  1:61017/1‑300 (MQ=255)                                                                                                                                                                               caccaGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAAc                                                                                                                >  2:260140/1‑299 (MQ=255)                                                                                                                                                                               caccaGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTAAAAGAATTATTTGaaaaagc                                                                                                               >  2:1260/1‑298 (MQ=255)                                                                                                                                                                                 ccaGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGt                                                                                                             >  1:51865/1‑300 (MQ=255)                                                                                                                                                                                 ccaGGACGACATACCGNCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑NCATTGTCGCAAGTCACAGNATTATTTGAAAAACCGt                                                                                                             >  1:768822/1‑300 (MQ=255)                                                                                                                                                                                    ggACGACATACCGTCACACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGGTTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCGCCGCTGGGCCATTGTCGCAAGt                                                                                                                                    >  1:320764/1‑277 (MQ=255)                                                                                                                                                                                    ggACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGc                                                                                                          >  1:802773/1‑300 (MQ=255)                                                                                                                                                                                    ggACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAAAACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGc                                                                                                          >  1:298376/1‑300 (MQ=255)                                                                                                                                                                                        gacATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGCAAAACCGTTGCTGGa                                                                                                      >  2:744735/1‑300 (MQ=255)                                                                                                                                                                                         acATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCCGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGACA‑ACGTTGG‑AGATTGTCGCAAGTCAAAGAATTATTTGAAAACCCGTTGCTGGAt                                                                                                     >  2:511379/1‑300 (MQ=255)                                                                                                                                                                                         acATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCAACGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAATCGTTGCTGGAt                                                                                                     >  2:61259/1‑300 (MQ=255)                                                                                                                                                                                           aTACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATc                                                                                                    <  2:886461/299‑1 (MQ=255)                                                                                                                                                                                              ccGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATctgct                                                                                                >  2:188418/1‑300 (MQ=255)                                                                                                                                                                                                        aGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATACTCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAgc                                                                                       <  2:760388/300‑1 (MQ=255)                                                                                                                                                                                                        aGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAgc                                                                                       <  2:220999/300‑1 (MQ=255)                                                                                                                                                                                                                       gACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACCGCATTATTTGACAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATc                                                                         >  2:408428/1‑299 (MQ=255)                                                                                                                                                                                                                       gACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATc                                                                         >  2:840845/1‑299 (MQ=255)                                                                                                                                                                                                                       gACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCAC‑ACGCTGG‑ACATTGTAGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTATTTGAAGCGCAGCAGGTGCATc                                                                         >  2:407908/1‑299 (MQ=255)                                                                                                                                                                                                                        aCAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGc                                                                       <  1:154353/300‑1 (MQ=255)                                                                                                                                                                                                                        aCAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATGTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGc                                                                       <  1:155458/300‑1 (MQ=255)                                                                                                                                                                                                                                                        accGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTc                                         <  1:127597/298‑1 (MQ=255)                                                                                                                                                                                                                                                        accGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCA‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCGGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTc                                         <  1:258596/298‑1 (MQ=255)                                                                                                                                                                                                                                                           ggATAAACCTGCAGAGTGGA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGAGTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGTAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCAc                                    <  2:500672/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             gA‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCCACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGAc                  <  2:423547/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              gagatCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGAc                  <  1:604202/296‑1 (MQ=255)                                                                                                                                                                                                                                                                              a‑GGTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGAcc                 <  1:463429/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACANGTCTACACCGAATTAACAACAAAAAACACGTTNTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGAcc                 >  2:690864/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                 gTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGAcc                 >  1:78729/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                 gTCATGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGACTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAACTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGAc                  >  1:41248/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                    aTGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAg             <  1:731465/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                    aTGGATGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGc            <  1:96619/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        aTGTGTATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTAGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGt        >  2:435400/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          gtgtATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGCTTAAGACCGGAGCTTGTcc      >  2:114505/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          gtgtATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTcc      >  1:585046/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          gtgtATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGACAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACATTGCTGTCGATTAAGACCGGAGCTTGTcc      >  2:25712/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          gtgtATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGAAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTAAACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGAAAGTCAAAGAATTATTTGAAAAACCGTTGATGGATCTGCTGTTTGAAGCACAGCAAGTGCATCGACAGCATTTCGATCCTCGTCAGGTGCAGGTAAGCACGTTGCTGTCGATTAAGACCAGAGCTTATcc      >  2:370816/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            gtATGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAGAGATTTAGG‑TTTACAAGTCTACACCGAATTAAAAACAAAAAACACGTTTTGGAGAAGCCACATGGCTCACCGCCC‑ACGCTGG‑ACATCGTCGAAAGTCACAGAATTATCTGACAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTcc      >  2:602465/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                              aTGGGTGCAGGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGTAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGaa  <  1:114505/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      ||                                                                                                                                                                                                                                                                                                         AGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACAT‑GCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCA‑ACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGA‑GGTCATGGATGTGTATGGGTGCCAGA‑TATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTT‑GTAAACC‑AAATTGAAAAGATTTAGG‑TTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCC‑ACGCTGG‑ACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAA  >  NC_000913/808927‑809508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑