breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 37 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,037,4960AT45.5% 68.4 / 253.0 135intergenic (+161/‑23)alaT/rrlAtRNA‑Ala/23S ribosomal RNA
*NC_0009133,911,5330CA44.3% 125.7 / 22.3 106intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009131,097,1180CA35.3% 12.7 / 13.4 17intergenic (+289/+447)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009131,502,7720GA32.4% 89.1 / 21.0 69A106T (GCG→ACG) ydcLDUF3313 domain‑containing lipoprotein YdcL
*NC_0009131,427,9920GT32.2% 181.5 / 13.8 121P245P (CCC→CCAinsH5IS5 family transposase and trans‑activator
*NC_0009131,502,7700TC31.9% 86.8 / 24.5 69I105T (ATA→ACA) ydcLDUF3313 domain‑containing lipoprotein YdcL
*NC_00091385,6510GT30.9% 133.0 / 12.2 94E8* (GAG→TAG) ilvIacetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
*NC_0009133,054,8860GT29.8% 101.7 / 13.5 67Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_0009133,741,8710GT28.7% 173.7 / 14.6 101S221Y (TCC→TAC) plaRDNA‑binding transcriptional repressor PlaR
*NC_0009132,477,2650AC27.9% 103.2 / 10.5 61L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009133,926,5950CA27.7% 141.8 / 13.2 83E137* (GAG→TAG) asnCDNA‑binding transcriptional dual regulator AsnC
*NC_0009134,249,8100CA27.4% 124.8 / 15.7 73P86Q (CCG→CAG) malMmaltose regulon periplasmic protein
*NC_0009133,620,8440TC27.3% 15.7 / 13.2 22R551R (CGT→CGCrhsBrhs element protein RhsB
*NC_0009131,480,7190GT27.0% 136.8 / 10.9 74E528* (GAG→TAG) ynbChydrolase/methyltransferase domain‑containing protein YnbC
*NC_0009134,296,2680TC26.9% 37.9 / 11.7 26intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_000913847,9550GA26.7% 177.9 / 32.6 105A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein
*NC_0009134,360,9650CA26.5% 228.1 / 16.0 118E324* (GAG→TAG) cadCDNA‑binding transcriptional activator CadC
*NC_0009132,490,2400GT26.1% 162.5 / 12.3 92intergenic (‑54/+16)yfdV/oxcputative transport protein YfdV/oxalyl‑CoA decarboxylase
*NC_0009133,620,8590CT25.9% 27.9 / 12.9 27R556R (CGC→CGTrhsBrhs element protein RhsB
*NC_0009133,620,8620CT25.9% 24.5 / 15.9 27F557F (TTC→TTTrhsBrhs element protein RhsB

Marginal new junction evidence (lowest skew 10 of 41 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 265341964 (0.830)14 (0.200) 13/538 3.1 17.8% noncoding (37/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653746 = 71 (1.010)intergenic (‑291/‑109) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 = 294746759 (0.770)10 (0.180) 7/422 3.8 17.5% intergenic (+4/‑30) metZ/metW tRNA‑Met/tRNA‑Met
?NC_000913 2947688 = 52 (0.940)intergenic (+5/+69) metV/amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
* ? NC_000913 = 235769088 (1.150)8 (0.110) 8/538 4.5 9.3% coding (787/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357874 = 76 (1.080)intergenic (+71/+168) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 = 4542690159 (2.070)13 (0.170) 8/570 4.7 9.2% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 103 (1.380)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 4542682 =148 (1.930)12 (0.160) 8/570 4.7 8.8% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 105 (1.410)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 78144645 (0.590)5 (0.090) 5/432 4.7 14.1% intergenic (+2/‑131) lysZ/lysQ tRNA‑Lys/tRNA‑Lys
?NC_000913 781655 = 28 (0.500)intergenic (+3/‑430) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 3599001358 (17.680)8 (0.110) 8/574 4.7 0.66% coding (412/471 nt) cynS cyanase
?NC_000913 360159 = 1076 (14.350)coding (168/1155 nt) cynX cyanate transporter
* ? NC_000913 = 439243754 (0.700)4 (0.070) 4/432 5.2 7.8% intergenic (+2/‑35) glyV/glyX tRNA‑Gly/tRNA‑Gly
?NC_000913 4392661 = 55 (0.970)intergenic (+3/‑231) glyY/yjeV tRNA‑Gly/uncharacterized protein YjeV
* ? NC_000913 = 235765283 (1.080)5 (0.070)
+GCGGCAGGAAGGACG
5/558 5.8 5.9% coding (749/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357851 = 85 (1.110)intergenic (+48/+191) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 = 3469405102 (1.330)4 (0.060) 4/556 6.2 3.9% coding (449/2694 nt) chiA endochitinase
?NC_000913 3469715 = 101 (1.390)coding (139/2694 nt) chiA endochitinase