| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,054,886 | 0 | G | T | 29.8% | 101.7 / 13.5 | 67 | Q187K (CAA→AAA) | ygfB | PF03695 family protein YgfB |
| Reads supporting (aligned to +/- strand): ref base G (18/29); new base T (20/0); total (38/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAG‑CTTCACCGGTTTCGCCGGTCACTTTATCCAGC‑TTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCC > NC_000913/3054598‑3055168 | aaCGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGgcg < 1:506717/300‑1 (MQ=255) aaCGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt < 2:626238/300‑1 (MQ=255) aCGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCAACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCTGTCGGTTGctggt > 1:64598/1‑295 (MQ=255) cTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTA‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGGCGGTTTTTGTCCTTc > 2:77859/1‑276 (MQ=255) gtgGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTCCTTTTTGCGCGGTCAGTTTTGGATGAGTaa > 2:584383/1‑300 (MQ=255) ggTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTAAGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTGTAcctc > 2:145178/1‑269 (MQ=255) gTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAgg < 1:439171/300‑1 (MQ=255) gTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAgg < 1:456578/300‑1 (MQ=255) gCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCCGACGGCGACGCTGAAACTCTTGCCGGGATATCCC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAAc < 2:597805/299‑1 (MQ=255) gCTTTTAATCAGCACCAACACCGCTTCCGGTTCGTTAAAGCCGTTGAAGTACCAGAAGTCACTGTTCGGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAAc < 2:362043/299‑1 (MQ=255) tttgaaTCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAg < 2:574628/295‑1 (MQ=255) ttgaaTCAGCACCATCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCTGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAg < 2:290820/295‑1 (MQ=255) accGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTTCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTTTCGTGGCATAACAGCGCTGCAACACGAACGt < 2:82646/299‑1 (MQ=255) ccGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAANCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCANCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCCTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCACAACAGCACGGCAACACAAACGt > 2:507476/1‑298 (MQ=255) gTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACCCGAACGTATTTGATGGtctc > 1:463043/1‑298 (MQ=255) aGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGc < 1:29893/300‑1 (MQ=255) gTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCCTCTGGCGCGGTCAGtt > 1:405651/1‑227 (MQ=255) atGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCGGTTGCGGATGAGTAAAAGTGTCGTGGCa > 1:405657/2‑252 (MQ=255) aCTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGGTTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGAt < 2:348640/300‑1 (MQ=255) gTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTACTTCTGGCGCGGTCGGTTGCGGGTGAGTAAAGNTGTCGTGGCATAACAGCGCGGCAACACAAACATATTCGATGATCTCTTCCAGCGA‑CATTTCCAACTCTTCCTTAtctt > 1:142796/1‑300 (MQ=255) gTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAcctc > 2:668215/1‑190 (MQ=255) gTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAACGCGGAAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGAtctt > 1:559959/1‑300 (MQ=255) gTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTGGCGCGGTCGGTTGCGAATGAGTAAAAGTGTCGTGGCACAACAACGCGGCAACACGAACGTATTCGATGATTTCTTCAAGCGA‑AACTTCCAACTCTTCCCGAtctt > 2:324338/1‑300 (MQ=255) tCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTCCTTCTTGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCACGGCAACACAAACATATTCGATGATCTCTTCCAACGA‑CATTTCCAACTCTTCCTGAtcttc > 1:609173/1‑299 (MQ=255) tGACGATAGGGGTATTCGCTGTCGGCGCTACTTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGTTTGCATTTGCTCCACCAGTGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTTTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGTATAACAGCGTGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTTAAGCTCTTCCTGAtc < 2:432341/294‑1 (MQ=255) ggggTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCACAACAGCGCGGCAACACGAACGTGTTCGATGATCTCTTCAAGCGA‑CACTTCAAGCTCTTTCTGATCTTCGTCTTcgtcgt > 1:644829/1‑300 (MQ=255) ggTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTNCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTGTCCTTCt > 2:493628/1‑180 (MQ=255) gCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTATAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGt < 2:475176/299‑1 (MQ=255) gcTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGt < 2:96331/300‑1 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGTTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAGGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGc < 2:373817/300‑1 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTNAAACTCTTGCCTGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGc < 1:145826/300‑1 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCGGTTGCGGATGAGTCAAAGTGTCGTGGAACAACAACACGGCAACACAAACGTANTCTATGATCTCCTCCAACGA‑CATTTCCAACTCTTCCCTATCTTCGTCTTCTTCTTAAccccatttcgaaatgttccgc > 1:128892/1‑282 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATCACAGCGCGGCAACACGAACGTANTCGATGATCTCTTCAAGCGA‑AACTTCCAACTCTTCCCGATCTTCGTCTTCGTCGTAACCCAATTGTGCAACGTTACGc > 1:484769/1‑300 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGAATAACAGCACAGCAACACGAACGGATTCGATGATCTCTTCAAGCGA‑CATTTCCAGCTCTTCCTGATCTTCGTCTTCGTCGTAAACCAATTGCGAAATGTTACGc > 1:103439/1‑300 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGc < 2:411797/300‑1 (MQ=255) ttACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTACCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTGAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGc < 2:27474/297‑1 (MQ=255) tACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAAcccctgtctcttatacacatctcgc < 1:56892/300‑23 (MQ=255) ggTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGNATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGAt < 2:482168/300‑1 (MQ=255) gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTGGCGCGGTCGGTTTCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACAAACGTATTCTATGATCTCNTCCAGCGAACCTTCCAAAC‑CTTCCTGATCTTCTTTTTCGTCGTCAACCAGttttgacatattcagcagattgtcaata > 1:139764/1‑274 (MQ=255) ggcgCTGCCTGTTTGTATTTGCTCCACCAGGGCCTGACGTCGACGCTTAAACTCTTGCCTGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTTTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGc < 2:179406/269‑1 (MQ=255) cTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTGCTTCt > 1:552366/1‑124 (MQ=255) ccaccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCt > 1:246289/1‑105 (MQ=255) accaGGTCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAG‑CTTCACCGGTTTCGCCGGTCACTTTATCCAGC‑TTCGGTTGCGt < 2:474074/300‑1 (MQ=255) accaGGGCCTGACGGCNACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCACAACAGCGCGGCAACACGAACGTATTCTCTGATCTCTTCCAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTCCGCAGATCGTCGATAG‑CTTCACNAGTTTCGCCGGTCACTTTNTCCCGC‑TTCGGTTGCGt > 1:568432/1‑300 (MQ=255) accaGGGCCTGACGGCNACGCTGAAACTCTTGCCGGGATATCTA‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAACGCAGCAACACAAACGTATTCTCTTACCTCTTCAAGCGA‑CACTTCAAACTCTTCCTGGTCTTCGTCTTCGTCGTGAACCAGTTGTGCAAGGTTACGAAGAGCGGCGATAGACTCCAN‑AGTTTCACCAGTCACCTTNTCCAGC‑TTCGGTTgtgt > 1:568424/1‑297 (MQ=255) accaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTGTACTTCTGGGGcggt > 2:405521/1‑111 (MQ=255) accaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCCACACAAACGTATTCGATGATCTCTTCCAGCGA‑CATTTCCAACTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAG‑CTTCACCAGTTTCGCCAGTCACTTTCTCCAGC‑TTCGGTTGCGt > 1:555908/1‑300 (MQ=255) accaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGAATAACAACGCGGCAACACGAACGTATTTGATGATCTCTTCAAACGA‑AATTTCAAACTCTTCCTGATCTTCGTCTTCGTCGTCACCCAGTTTCGCAATTTTTCGCAAATCGTTGACCG‑CTTCCCCGGTTTCGCCAGTCACTTTCTTCAAC‑TTCGGTTgtgt > 2:614111/1‑297 (MQ=255) accaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑AATCAT‑AACACTCTCATTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTGTCCTTCTTGCGCGGTCGGtt > 2:27254/1‑116 (MQ=255) gggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCCTCACAACGCGGCAAAACAAACATCTTCTATTATCTCCTCAAACGA‑CCCTTCCAACTCTTCCTGATCTTCGTCCTCATCATAACCCAATTGCGAAATGTTGCGCAGATCGTCGATAA‑ATTCACCAGTTTCGCCAGTCACCTTCCCCAAC‑TTCGGtttcatcaaa > 1:183488/1‑292 (MQ=255) gagcgccgactctgaacgctctTTCCGGGATATCTCGACTCATCGACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAG‑CTTCACCGGTTTCGCCGGTCACTTTATCCAGCGTTCGGTTGCGTAACGc < 1:235391/282‑1 (MQ=255) acgtcgactcttaaaCCCTTGCCGGGATATTTC‑GCTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGGGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTGAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCCCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAG‑CTTCACCGGTTTCGCCGGTCACTTTATCCAGC‑TTCGGTTGCGTAACGCCAAGAc < 2:269175/288‑1 (MQ=255) gCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCCTCTGGAGCGGTCGGTTGCGGATGAGTAAAAGTGTCGTGGAACAACAACGCGGCAACACGAACATATTCGATGATCTCTTCCAACGA‑AATTTCCAACCCTTCCTGATCTTCTTCTTCGTCGTAACCCAATTGCGCAATGTTACGCAGATCGTCGATAG‑CTTCACCGGTTTCGCCGGTCACTTTCTCCAGC‑TTCGGTTGTGTAAAGCCAAGACCAAGCa > 1:387993/1‑298 (MQ=255) cTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCt > 2:125042/1‑83 (MQ=255) gAAACTCTTGCCTGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAG‑CTTCACCGGTTTCGCCGGTCACTTTATCCAGC‑TTCGGTTGCGTAACGCCAAGACCAAGCAGGAAg < 1:506742/300‑1 (MQ=255) gAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAG‑CTTCACCGGTTTCGCCGGTCACTTTATCCAGC‑TTCGGTTGCGTAACGCCAAGACCAAGCAGGAAg < 1:506747/300‑1 (MQ=255) ggATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAG‑CTTCACCGGTTTCGCCGGTCACTTTATCCAGC‑TTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGAccc < 2:373382/297‑1 (MQ=255) atatCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCa > 1:538838/1‑104 (MQ=255) gtctc‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTGTACTTCTGGCGCGGTCGGTTGTGGATGAGTAAAGGTGTCGTGGCATGACAACTCGGCAACACAAACGTCTTTGATGATCTCCTCAAGCGA‑CACTTCCAGCTCTTCCCGATCTTCGTCTTCGTCGTAACCCCATTTCGCAATGTTACGCAAATCGTCGATAG‑GTTCACCGGTTTCGCCGGTCACCTTCTCCAAC‑TTCGGTTGCGTAACGCAAAAAACAAGCAGGAAGTGATTGCCCCAAACTGc > 2:669787/2‑300 (MQ=255) actcaT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCTTCt > 2:292315/1‑59 (MQ=255) ctcaT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGGGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAACACGGCAACACAAACATACTCGATGATCTCTTCCAACGA‑CACTTCAAGCTCCTCCTGACCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAAATCGTCGATAG‑CTTCCCCGGTTTCGCCAGTCACTTTATCCAGC‑TTCGGTTGCGTAACGACAAGACCAAGCAGGAAGTGATTGACCCAACCCGCCAATGc > 1:559780/1‑300 (MQ=255) caT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTGTCCTTCt > 1:188053/1‑56 (MQ=255) caT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAACGCGGCAACACGAACGTATTCGATGATCTCTTCAAACGA‑CACTTCCAACTCTTCCTGATCTTTGTCTTCGTCGTAAACCAATTGCGCAATGTTACACAGATCGTCGATAG‑CTTCCCCAGTTTCGCCGGTCACTTTATCCCGC‑TTCGGTTGc > 1:680020/1‑251 (MQ=255) acacTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCtt > 1:306628/1‑52 (MQ=255) acTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAG‑CTTCACCGGTTTCGCCGGTCACTTTATCCAGC‑TTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGcc < 2:607706/299‑1 (MQ=255) gTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAG‑CTTCACCGGTTTCGCCGGTCACTTTATCCAGC‑TTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGAcat < 2:169572/300‑1 (MQ=255) gtgtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAG‑CTTCACCGGTTTCGCCGGTCACTTTATCCAGC‑TTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATTGcc > 1:170246/1‑300 (MQ=255) | AACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTC‑ACTCAT‑AACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGA‑CATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAG‑CTTCACCGGTTTCGCCGGTCACTTTATCCAGC‑TTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCC > NC_000913/3054598‑3055168 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |