breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 36 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913941,7130TG39.5% 63.1 / 12.8 38V252G (GTG→GGG) dmsAdimethyl sulfoxide reductase subunit A
*NC_0009131,554,0000TG35.2% 64.1 / 10.2 54Y557S (TAC→TCC) maeAmalate dehydrogenase (oxaloacetate‑decarboxylating)
*NC_0009131,315,8840CT33.3% 59.4 / 11.1 37S51N (AGC→AAC) yciGstress‑induced bacterial acidophilic repeat motifs‑containing protein YciG
*NC_0009132,001,9110GA33.3% 52.6 / 14.2 36intergenic (‑122/+1)fliA/fliXRNA polymerase sigma factor FliA/small RNA FliX
*NC_0009131,413,7350AT32.3% 57.9 / 13.3 31Y2* (TAT→TAA
*65K (TAA→AAA) 
rcbA
ralR
double‑strand break reduction protein
endodeoxyribonuclease toxin RalR
*NC_000913343,4440AG32.2% 108.3 / 13.3 59G187G (GGA→GGGyahKaldehyde reductase, NADPH‑dependent
*NC_0009133,003,9210TG31.8% 142.6 / 13.5 63Y145D (TAT→GAT) xdhCputative xanthine dehydrogenase iron‑sulfur‑binding subunit XdhC
*NC_0009131,026,4460CG31.7% 95.4 / 13.0 42A659G (GCA→GGA) helDDNA helicase IV
*NC_0009133,177,7480CA31.7% 65.6 / 11.7 41E55* (GAG→TAG) nudFADP‑sugar pyrophosphatase
*NC_0009134,604,3440CT30.9% 66.4 / 12.0 42S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009133,505,0880CT30.8% 55.3 / 10.3 40S311S (TCG→TCAyhfSputative aminotransferase YhfS
*NC_0009132,950,2010TG30.3% 135.8 / 10.0 66G320G (GGT→GGGargAN‑acetylglutamate synthase
*NC_000913634,3150AG30.0% 95.2 / 13.1 50K244E (AAA→GAA) ybdLmethionine transaminase
*NC_0009132,465,5410CT29.8% 82.6 / 12.3 47Q81* (CAA→TAA) yfdCinner membrane protein YfdC
*NC_0009133,947,8500CT29.8% 89.3 / 16.7 47E40K (GAA→AAA) hdfRDNA‑binding transcriptional dual regulator HdfR
*NC_0009133,830,3250GA29.5% 64.9 / 12.9 44E461K (GAA→AAA) xanPxanthine:H(+) symporter XanP
*NC_0009133,697,7400TG28.6% 170.7 / 15.5 77V428G (GTG→GGG) bcsEc‑di‑GMP‑binding protein BcsE
*NC_0009132,851,6540TG28.4% 175.4 / 10.2 75G218G (GGT→GGGhypBhydrogenase isoenzymes nickel incorporation protein HypB
*NC_0009133,556,2000GA27.8% 59.5 / 11.8 37S217F (TCC→TTC) rtcARNA 3'‑terminal phosphate cyclase
*NC_0009134,161,1420CA27.1% 100.9 / 14.1 48Q26K (CAA→AAA) fabRDNA‑binding transcriptional repressor FabR

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 125036156 (0.940)3 (0.050) 3/490 7.1 5.1% coding (478/1071 nt) dhaK dihydroxyacetone kinase subunit K
?NC_000913 = 1307952 55 (0.930)coding (694/1461 nt) clsA cardiolipin synthase A
* ? NC_000913 = 98684341 (0.690)3 (0.050) 3/490 7.1 5.6% coding (140/1089 nt) ompF outer membrane porin F
?NC_000913 4132521 = 60 (1.010)intergenic (+254/‑95) metL/metF fused aspartate kinase/homoserine dehydrogenase 2/5,10‑methylenetetrahydrofolate reductase
* ? NC_000913 = 58953385 (1.430)3 (0.050) 3/490 7.1 3.9% coding (1422/2973 nt) nfrA exopolysaccharide secretion system outer membrane protein NfrA
?NC_000913 1509348 = 62 (1.040)coding (63/177 nt) hicA mRNA interferase toxin HicA
* ? NC_000913 = 2361788 (1.480)3 (0.050) 3/490 7.1 3.1% coding (1227/2817 nt) ileS isoleucine‑‑tRNA ligase
?NC_000913 = 3381914 99 (1.670)coding (1172/1368 nt) degQ periplasmic serine endoprotease