| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,851,654 | 0 | T | G | 28.4% | 175.4 / 10.2 | 75 | G218G (GGT→GGG) | hypB | hydrogenase isoenzymes nickel incorporation protein HypB |
| Reads supporting (aligned to +/- strand): ref base T (11/42); new base G (21/0); total (33/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.52e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTGAAGGCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGAC > NC_000913/2851409‑2851902 | aTTGAAGGCCACCAGCACACCGGGAACGATGCCGCTCGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGACCACCGGTAAAGGCTGCCATCTTGACGCACAGATGAGTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCGCGTATGCCCGGCCAGCTGCGATCTCGGGGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAg < 2:304187/250‑1 (MQ=255) aaGGCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACaaa < 1:155131/251‑1 (MQ=255) aaGGCAACCAGCAAACCGTTAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACaaa < 1:355830/251‑1 (MQ=255) gCGACCAGCAAACCGTGAACGATGCCGCACGCATTCTCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCa < 2:331945/251‑1 (MQ=255) aaaCCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAatat < 1:338031/251‑1 (MQ=255) aCCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATcc < 1:246344/251‑1 (MQ=255) gTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCCTTACCG‑ACGGGGAAAACAAACCACTGAAATATCCGc > 1:76097/1‑250 (MQ=255) tGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTTCCG‑AAGGGGAAGACAAACCACTGAAATATCCGCat > 1:27737/1‑251 (MQ=255) caaCGATGCCTCACGCATTCGCGCTACCGGCCCACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCat < 1:243298/249‑1 (MQ=255) aCGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATg < 1:292519/251‑1 (MQ=255) aCGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCCTTTCCG‑CAGGGGGAGACAAAACACTGAAATATcaccaaaat > 2:437699/1‑243 (MQ=255) cGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGCGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACGGAAAAATCCGCCTATGt > 1:560109/1‑251 (MQ=255) gATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGtt < 2:76097/251‑1 (MQ=255) attccGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGttt < 2:6007/248‑1 (MQ=255) aTGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGA‑TTTTCGTTACCG‑AAGGGGGAGACaaaaaaacgaaaaatcaccatatgttt > 2:240223/1‑227 (MQ=255) gcaTTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTTCCG‑CAGGGGAAGACAAACCACTCAAatataccccaatgttttccgccccc > 2:95877/1‑230 (MQ=255) cgcgCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCt < 1:240223/251‑1 (MQ=255) gcTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGAt < 1:377794/251‑1 (MQ=255) gCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGTCGCACAGATGATTGCTGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTc < 1:328435/251‑1 (MQ=255) acacCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGG‑TTTCCCGTACCC‑AAGGGGGAGAAAAAACACCGAAAAATCCGCATATGTTTTCCGCCCCCCCGCTGAtgcttcgccc > 2:107135/1‑245 (MQ=255) gCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGt < 1:173164/251‑1 (MQ=255) cGATTCAGGTGAACACCGGTAAAGGCTTCCATCTTGACGCACAGATGATTGCCGACGCCGCTCCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGtt < 1:151911/251‑1 (MQ=255) cAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGGGAAAACAAACCACCGAAAAATCCGCATTTGTTTGCCGCCCCCCCGCTGATGCTGCTCCACAAAGTTTAccc > 1:339462/1‑250 (MQ=255) cAGGGGAACAGCGATAAAGGCTGCCCTCTTGCCTCACAGATGATTGCCGACGCCGCGCCGTGTCTGCCACTTGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTGGGTGAAAAACTAAAAGGGGCGGTGC‑TACCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAGAGTTGACC‑t < 2:426466/251‑1 (MQ=255) aaCACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGcc < 2:270104/251‑1 (MQ=255) cacCGGTAAAGGCTGC‑A‑CTGGACGCACAGAGGAGTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTGCCGTTACCG‑AAGGTGAAGACAAACCACGGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTAt < 2:44006/251‑1 (MQ=255) cGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCCTTACCG‑AAGGGGAAAACAAACCACTGAAATATACGCCTAAGTTTTCCGCCGCCCCGCCGATTCTGCTACACCAAGTTGACC‑TGTTGCCggttc > 1:376775/1‑247 (MQ=255) ggTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTTCCG‑AAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCCCGCTGATGCTGCTCCACAAAATTGAAC‑TGTTGGCGTAtct > 1:330581/1‑251 (MQ=255) gTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCCGTACCC‑GAGGTGAAGACAAACCACCGAAATATCCGCATCTGTTTGCCGCCGCCCCCCTTATGCTGCTCAACAAAGTTTACC‑TGTTGCCggttctc > 1:462089/1‑245 (MQ=255) aaaGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑Ca > 1:51037/1‑250 (MQ=255) gCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTACCC‑AAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCCCCGATGCTGCTCCACAAAAATGACC‑TGTTGCCGTATCT‑CAA‑ATTTGAc > 1:394654/1‑251 (MQ=255) cacaGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATAGGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTGGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGc < 1:343069/251‑1 (MQ=255) caGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCAt < 2:394654/251‑1 (MQ=255) caGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCAt < 1:242815/251‑1 (MQ=255) aGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATc < 1:241653/251‑1 (MQ=255) gatgatTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑CTTCCGTTACCC‑AAGGGGAAGACAAACCACTGGAAAATCCCCCTATGTTTTTCGCCGCCTCCCTCATGCTCCTCAACAAAATTGACC‑TGTTGCCGGTTAT‑CCA‑CTTTGACGTTGAAAAGGGCATCg > 1:97506/1‑251 (MQ=255) gatTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCCTATGTTTGCCGCCCCCTCGCCGATGCTGCTCAACAAAGTTGACCCTGTT‑CCGGATCT‑CAA‑CTTTTACGTTGGAAAGAGCATCGccc > 1:456748/1‑250 (MQ=255) gACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTTCCG‑AAGGGGAAGACAAAACACTGAAATATCCGCATATGTTTGCCGCCCCCTCGCTTATGCTGCTCAACAAAGTTGACC‑CGTTTCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGcccc > 1:43611/1‑250 (MQ=255) ccgcACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGaa < 1:95877/251‑1 (MQ=255) cgcACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGG‑CTTCCCTTACCCCAAGG‑GAAGAAAAACCACTGTAAAAACCGCATATGTTTGCCGCCCCCTCGCTGATGCTGCTCAACAAAATTTATC‑TTTTGCCGGAACTTCAA‑‑TTTTACGTTGAAAAATGCATCGCCTGCGCCcgcgga > 2:475909/1‑248 (MQ=255) tGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTTCCG‑CAGGGGAAGACAAAACACTGAAATATCCGCATATGTTTGCCGCCGCCCCGCTGATGCTGCTCAACAAAATTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGAGCATCGCCTGCGCCCGCGAAGTCAATTCAGa > 1:431757/1‑250 (MQ=255) gCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAg < 2:79557/248‑1 (MQ=255) gCCACTGGACGATAACGGTATTCGGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGa < 1:382850/249‑1 (MQ=255) aaCGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATcc < 2:560109/250‑1 (MQ=255) aaCGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCt < 2:135604/251‑1 (MQ=255) gTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGaa < 2:7814/197‑1 (MQ=255) gTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAATTGACC‑TGTTGCCGTATCT‑CAA‑CTTTTACGTTGaa > 1:7814/1‑197 (MQ=255) aTTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCGCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTAttt < 1:23470/250‑1 (MQ=255) ttCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGGGAAGACAAAACACTGAAATATCCGCATATGTTTGCCGCCGCCCCGCTGATGCTGCTCAACAAAATTGACC‑TGTTGCCGTCTCT‑CAA‑CTTTGACGTTTAAAAGTGCATCGCCTGCGCCCGCGAAGTCAAACCAGAAATTTAAATCATCCTTATTTcc > 1:430170/1‑251 (MQ=255) ttATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGccac < 2:339462/250‑1 (MQ=255) cGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTTC‑TTTCCTTTACCC‑AAGGGGAAGACAAACCACTGAAAAATCCGCATATGTTTTCCGCCCCCTCGCTGATGCTGCTCAACAAAGGTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTTCATCCCCTGCGCCCGCCAAGTCCATTCCGGAATTGAAATCATCCTTATTTTCGCCCCCAgcg > 1:29814/1‑251 (MQ=255) gTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTc < 2:62671/122‑1 (MQ=255) gTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGAGC‑TTTCCGTTACCG‑AAGGTGAAGAAAAACCACTGAAATATCCGCATATGTTTTCCGCCGCCTc > 1:62671/1‑122 (MQ=255) tGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAggg < 1:112257/250‑1 (MQ=255) ggCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCCTTTCCCTT‑CCG‑ACGGGGGAGGAAAACCAAATCAATAATCGCATCTGTTTGCCGCCGCCTCGCTGGTGCTGCTCAACCAAGATGAAC‑CGCTGCCCTATCC‑CAAACTTT‑ACGGTTAAAAGGGCATCACCTGCGGCCGCGAAGGCAATCCAGAAATTGAAATCATCCTTTTTTCCGCCCCCAccgggccaggggt > 2:409314/1‑242 (MQ=255) tCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGGGAAAACAAAACACTGAAATATTCGCCTATGTTTTGCGCCGCCTCCCCGATGCTGCTCAACAAAATTGACC‑TGTTGCCGTATCT‑CAA‑CTTTTACGGTGAAAAGTGCCTCGCCTGCGCCCGCGAAGACAATCCACAAATTGAAATCATCCTTATTTTCGCCCCCCACGGCGGAGGGGTGGaaca > 2:126052/1‑247 (MQ=255) cGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGt < 1:5877/251‑1 (MQ=255) tATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGGGGAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAATGCATCGACTGCGCCCGCGAAGCCAATCCAGAAAATGAAATCATCCTTATTTCCGCCACCAACGGCGAAGGGATGGACCAGTgg > 1:160388/1‑251 (MQ=255) cccGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAAAATCCGCATATGTTTGCCGCCGCCCCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGGAAAGGGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCAACCTTATTTCCGCCACCAGCGGCGAAGGGAAGGaacaagggctgg > 1:24563/1‑240 (MQ=255) ggCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAAct < 2:456748/251‑1 (MQ=255) cGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCC‑CAGGGGAAGAAAAACCACTGAAAAAACCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACCAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTTGCGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATTCTTATTTCCGCCCCCCGCGGCGAAGGGATGGACCAGTGGCTTAActggctgggg > 2:368484/1‑248 (MQ=255) cGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGacac < 1:107135/248‑1 (MQ=255) cGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAg < 1:2035/250‑1 (MQ=255) aaaaaCACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGGGAAAAAAAACCACCGGAATATCCGCCTATTTTTGCCGCCGCCCCGCCGATGCTGCTCCACAAAGTTGACC‑CGTTGCCGTATCT‑CAA‑CTTTGACGTTGGAAAATGCATCGCCCGCGCCCCCGCAGTCAATCCAGAAAATGAAAACATCCTTATTTCCGCCCCCCCCGGCGAAGGGATGGACCAAAGGCTGAActggctggggaacccacgaggg > 2:234651/1‑237 (MQ=255) cacaAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATAGCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCG‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATa < 1:140422/251‑1 (MQ=255) aaaGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGc < 2:148439/251‑1 (MQ=255) aaaGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGc < 2:339578/251‑1 (MQ=255) aggtgCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGTACCAGTGGCTGAACTGGCTGGAGACGCAGCGATTTGCATAGGCGt < 2:462089/247‑1 (MQ=255) ggCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTG‑CTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTccc < 2:27737/251‑1 (MQ=255) gCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTccc < 1:344103/251‑1 (MQ=255) gTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGc > 1:225222/1‑251 (MQ=255) gTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTAGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGGGCATAGGAGTTCCCGGc > 2:346782/1‑251 (MQ=255) gC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGcc > 2:558098/1‑250 (MQ=255) ccGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATcc > 1:106380/1‑251 (MQ=255) ggTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGAc > 2:528117/1‑251 (MQ=255) | ATTGAAGGCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCG‑AAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACC‑TGTTGCCGTATCT‑CAA‑CTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGAC > NC_000913/2851409‑2851902 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |