Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,947,8500CT29.8% 89.3 / 16.7 47E40K (GAA→AAA) hdfRDNA‑binding transcriptional dual regulator HdfR
Reads supporting (aligned to +/- strand):  ref base C (8/25);  new base T (14/0);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 9.36e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACTGTAAGCCTGTATGGGCATCCTGATTTTGATACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAATACTCCGCGCCATAACTAGCTCGGTCAAAGAATTAGGAGCGTGCAGGATGGCGGAAAGCTTT  >  NC_000913/3947600‑3948100
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                              
aaCTGTAAGCCTGTATGGGCATCCTGATTTTGATACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑c                                                                                                                                                                                                                                                                <  1:49002/251‑1 (MQ=255)
 aCTGTAAGCCTGTAGGGGCATCCGGATTTTGAGACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCTATAGAAAACTCGTTATGTCGTGAGGGATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑cc                                                                                                                                                                                                                                                               <  2:556077/251‑1 (MQ=255)
    gTAAGCCTGTATGGGCATCCTGATTTTGATACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGt                                                                                                                                                                                                                                                            <  1:514202/251‑1 (MQ=255)
      aaGCCTGTGTGGTCACGCTGTTTTTGATACAAGCGTTCCAGCCGCTGATTAAGCGTACATTCCCACACCGAGGCGCTGGAACATATAGAAAACTCGTTATTGCGTGAGGTATGCGCCCCCTCCTTTCTGGCGGCCTGCCAGGTGCTCATGATCGTTTCTGCTTAAGGCAGTAGTTTTTCGCCGGCAGCGGTTAAGCGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTA‑CCAGTTg                                                                                                                                                                                                                                                          <  2:464196/251‑1 (MQ=255)
        gCCTGTATGGGCATCCTGATTTTGATACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTc                                                                                                                                                                                                                                                        <  1:208210/251‑1 (MQ=255)
                                  aCAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTg                                                                                                                                                                                                                               <  1:466924/250‑1 (MQ=255)
                                           ccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAA‑GGTT‑ACACCCCGTTGATTTT‑TCAGTTGTCTTGTTTGCAAACTCACCGCTGACTGGGTCAG‑Attg                                                                                                                                                                                                                    >  1:226910/1‑249 (MQ=255)
                                                        aaGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGcc                                                                                                                                                                                                        <  1:428893/250‑1 (MQ=255)
                                                                  tCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGG‑AAAA‑AGTTCACACCCAGCTGGTTTT‑TCAGTTTTCTGGTTTGGAAACTCACTGCCGACTGGGGCAGGAT‑GAGCGGTTCAGCCCCTCGAccca                                                                                                                                                                                              >  2:451417/1‑248 (MQ=255)
                                                                    ccACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGt                                                                                                                                                                                          <  1:104558/251‑1 (MQ=255)
                                                                       caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGAc                                                                                                                                                                                       <  1:456950/251‑1 (MQ=255)
                                                                       caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGAc                                                                                                                                                                                       <  1:413604/251‑1 (MQ=255)
                                                                            aGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑GAAA‑AGTTGCCCCCCACTTGATTTT‑TCACTTTTCTTGTTTGAAAAGTCACCTCCGGCTTGGTCAG‑AAAGAGCGATTCAGCCGCTCgcacaaagggaaccgtt                                                                                                                                                                                   >  1:315813/1‑235 (MQ=255)
                                                                             ggCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGTCTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTc                                                                                                                                                                                 <  2:184027/251‑1 (MQ=255)
                                                                             ggCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTc                                                                                                                                                                                 <  1:451417/251‑1 (MQ=255)
                                                                                     cACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGtt                                                                                                                                                                                  <  2:111743/242‑1 (MQ=255)
                                                                                     cACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGG‑AAAAAGGTT‑ACACCCAGTTGATTTT‑TCCGTTGTTTGATTCGAAAGCTCACTGCTGACTTGGGCAG‑ATAGAGCGATTCAGCCGCCCGACC‑AAAATGACGCGttagatcggg                                                                                                                                                                          >  1:111743/1‑242 (MQ=255)
                                                                                              aaaaCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGG‑AAAA‑AGTTTCCCCCCAATTGGTTTT‑TCAGCTGTTTGCTTCGCAAACTCCAAGCTGACCGGGGGCG‑AGAGAGCGACTTAGCCGCCCCCCCCAAAG‑GACGCGTTCGGCTGACTTCCAGGaa                                                                                                                                                                 >  2:188312/1‑250 (MQ=255)
                                                                                                                         ccACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGG‑AAAA‑GGGTCACACCCAGGTGATTTT‑TCAGTTGTCTGATTCGAAAGCTTACTGCTGACTGGGGCAG‑ATAGAGCGGTTCAGCCGCCCGACC‑AAAGAGACGCGTTCGGCTAACTTCCAGGAAAG‑ATTTTTACAA‑TTCCGTATCCACAGTg                                                                                                                                   >  1:382280/1‑251 (MQ=255)
                                                                                                                               cctTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCtctc                                                                                                                              <  1:352003/250‑1 (MQ=255)
                                                                                                                                ctTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAA‑GGT‑CAAACCCAGGTGATTTT‑TCAGTTTTGTGAGTCGAAAGCTAAATGCTGACTGGGTCAG‑ATAGAGAGATTTAACCCCTCCACC‑ACAACGACGGGGTTGGCCAACTTTCCGGAAAG‑TTTTTTACAAATTCC‑TATCCACAGGGATCTccc                                                                                                                            >  1:431484/1‑250 (MQ=255)
                                                                                                                                 ttACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑AGGTCACACCCCGCTTATTTT‑TCAGTTGTTTGATTCTAAAACTCCCTGCTGGCTGGGGCAG‑ATAGAGAGATTCAGCCGCTCGACC‑CAAAAGACGCGTTTCGCTAACTTCCAGGAAAGGTTTTAAAAAA‑TTCC‑TATCcaccagggtctcccc                                                                                                                           >  1:555791/1‑239 (MQ=255)
                                                                                                                                      ggcggcCTGCCAGTTTCTCATGATCGTTTCTGCATTAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAat                                                                                                                      <  1:37344/251‑1 (MQ=255)
                                                                                                                                      ggcggcCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCGCAGTGCTCTCCGCAAAat                                                                                                                      <  1:331234/251‑1 (MQ=255)
                                                                                                                                                 aGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGG‑AAAAAGGTT‑ACACCCAGTTGATTTT‑TCCGTTGTCTGATTTGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGGTTCAGCCGCTCGACC‑AAAGGGACGCGTTCGGCTAACTTTCCGGAAAG‑GTTTTTACAA‑ATTCGTATCCCCAGTGCTCTCCGCCAAATTATTTTTCGtt                                                                                                           >  1:295836/1‑251 (MQ=255)
                                                                                                                                                       tCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑AGGTCACACCCCGGTGATTTT‑TCAGTTGTCTGATTTGAAAGCTCACTGCTGGCTGGGGCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGGTTGGCTAACTTCCAGGAAAG‑GTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTTTCGTTTTGAtt                                                                                                     >  1:234766/1‑251 (MQ=255)
                                                                                                                                                                   ntGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGGTCACACCCACTTGATTTTTCTTGTTG‑CTTATTCGAAAGCTCACAGCTGACCGGGGCAG‑GTAGAGGGATTTAGCCGCCCCACC‑AAAGTGACGCGTTTGGCTCACCTCCCGGAAAG‑ATTTTTACAA‑ATCCGTATCCCCAAGGCCCCCCGCAAAATTATTTTTCGTTTTTATTTTAAtgtttttt                                                                                         >  2:248345/2‑249 (MQ=255)
                                                                                                                                                                       aTAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GAGTCACACCCAGTTGATTTT‑TCTGTTGTTTGATTCGAAAGCTCACTGCTGACCGGGTAAG‑ATAGAGCGATTCAGCCGCCCCACC‑CAAGCGACGGGGTCGGCTAACTTCCAGGGAAG‑ATTTTAACAA‑ATCCGTATACACAGTGCTCTCCGCAAAATTATTTTTCGTTATGATTTAAATgttttgttttt                                                                                     >  2:490133/1‑250 (MQ=255)
                                                                                                                                                                        tAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGGTCACACCCAGTTGATTTT‑TCAGTTGTCTGATTCGAAAACTCCCTGCTTACTGGGTCAG‑ATAGAGCGATTCAGCCGCCCGACC‑AAAGTGACGCGCTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCCCAGTGCTCTCCGCAAAATTATTTGTCGTTTTGATTTAAATgttttgtttttt                                                                                    >  1:293431/1‑249 (MQ=255)
                                                                                                                                                                         aaGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTac                                                                                    <  1:170822/250‑1 (MQ=255)
                                                                                                                                                                          aGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTacac                                                                                  <  1:538438/251‑1 (MQ=255)
                                                                                                                                                                               gtagtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGt                                                                             <  1:189444/251‑1 (MQ=255)
                                                                                                                                                                               gtagtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑AGATCACACCCACGTGATTTT‑CTCGTTTTTTGTTTAGAAAGCTCACCCCTGGCTTGGTGAG‑ATAGAGCGATTCAGCCGCCCCGCC‑AAAGTGACGCGGTCGGCCAACTTCCCGGAAAG‑GTTTTTACAA‑TTCCGTATCCCCAGTGCTCTCCGCCACATTATTTTTTGTTATGATTTTAATgtttttttttttactcttt                                                                             >  2:441994/1‑242 (MQ=255)
                                                                                                                                                                                        cACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTa                                                                     <  2:295836/250‑1 (MQ=255)
                                                                                                                                                                                            ggCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGG‑AAAA‑AGATCACACCCCGTTGATTTT‑CCAATTGTCTGATTCCAAAGCTCACTTCTGACTGGGGCAG‑CTAAAGCGATTTCGCCGCCCGACC‑AAAAGGACGCGCTCGGCCAACTTTCCGGAAAGGTTTTTAA‑AA‑ATCCGTATCCACAATGCTTTCCGCCAAATTTTTTTTCGTTTTGATTTTAATttttttttttttaccctgtcactccgaaact                                                                 >  1:362558/1‑229 (MQ=255)
                                                                                                                                                                                             gCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAAt                                                               <  2:181809/251‑1 (MQ=255)
                                                                                                                                                                                                 cGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAATACTc                                                           <  1:382857/251‑1 (MQ=255)
                                                                                                                                                                                                     tAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAATACTCcgc                                                        <  1:490133/250‑1 (MQ=255)
                                                                                                                                                                                                     tAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAATACTCcgc                                                        <  1:188312/250‑1 (MQ=255)
                                                                                                                                                                                                       aaCGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTTCCACCCCGTTTATTTT‑TCAGTTGTCTTATTTGAAAGATCACTGCTGACTGGGGCAG‑ATAGAGCGATTCAGCCGCCCGACC‑AAAAGGACGCGGTCGGCTAACTTCCAGGAAAG‑TTTTTTACAA‑TTCCGTCTTCACAGTGCTCTCCCCCAAATTATTTTTCGTTTTGATTTAAATGTTTTGTTTTACACTCTTTCAAGCGTAACTAATACTCCGCGcc                                                     >  1:237222/1‑251 (MQ=255)
                                                                                                                                                                                                         cGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAATACTCCGCGCCAt                                                   <  1:35031/251‑1 (MQ=255)
                                                                                                                                                                                                                   ttCTGTGGCGGGGG‑AAAA‑AGTTCACACCCAGTTGATTTT‑TCAGTTTGCTGATTTGAAAACTCACTGCTGACTGGGTCAG‑ATAGAGCGATTTAGCCCCTCGGCC‑CAAATGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTTACAA‑TTTCCTATCCCCAGTGCTCTCCGCCAAATTATTTTTCGTTATGATTTTAATGTTTTTTTTTTCACTCTGTCAAACGTAACTAATACTCCGCGCCCTAACTAGCTcc                                         >  2:297127/1‑250 (MQ=255)
                                                                                                                                                                                                                        tgGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGAGACGCGTTCGGCTAACTTCCAGGAAAG‑GTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAATAATCCGCGCCATAACTACCTCGGTCa                                     >  2:350619/1‑250 (MQ=255)
                                                                                                                                                                                                                          gCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAATACTCCGCGCCATAACTAGCTCGGTCaa                                    >  1:346478/1‑249 (MQ=255)
                                                                                                                                                                                                                             ggTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAATACTCCGCGCCATAACTAGCTCGGTCAAAGAAt                               <  2:234766/251‑1 (MQ=255)
                                                                                                                                                                                                                              gTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAATACTCCGCGCCATAACTAGCTCGGTCAAAGAAtt                              <  1:451976/251‑1 (MQ=255)
                                                                                                                                                                                                                                          tCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAATACTCCGCGCCATAACTAGCTCGGTCAAAGAATTAGGAGCGTGc                    >  1:262960/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 cAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAATACTCCGCGCCATAACTAGCTCGGTCAAAGAATTAGGAGCGTGCAGGATgg             >  2:284484/1‑251 (MQ=255)
                                                                                                                                                                                                                                                           t‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAATACTCCGCGCCATAACTAGCTCGGTCAAAGAATTAGGAGCGTGCAGGATGGCGGAAAGCtt   >  2:436653/1‑251 (MQ=255)
                                                                                                                                                                                                                                                             ccAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAATACTCCGCGCCATAACTAGCTCGGTCAAAGAATTAGGAGCGTGCAGGATGGCGGAAAGCttt  <  2:559985/251‑1 (MQ=255)
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                              
AACTGTAAGCCTGTATGGGCATCCTGATTTTGATACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTG‑AAAA‑GGTTCACACCCAGTTGATTTT‑CCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAG‑ATAGAGCGATTCAGCCGCTCGACC‑AAAGTGACGCGTTCGGCTAACTTCCAGGAAAG‑TTTTTAACAA‑TTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGATTTAAATGTTTTGTTTTACACTCTGTCAAGCGTAACTAATACTCCGCGCCATAACTAGCTCGGTCAAAGAATTAGGAGCGTGCAGGATGGCGGAAAGCTTT  >  NC_000913/3947600‑3948100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑