breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 498,116 C→T P21L (CCT→CTT)  hemH → ferrochelatase
RA 498,809 A→G K252R (AAA→AGA)  hemH → ferrochelatase
RA 499,060 T→G D305A (GAC→GCC)  aes ← acetylesterase
MC JC 499,082 Δ52,248 bp [aes][ybcF] 46 genes
RA 803,662 C→A L54I (CTT→ATT)  ybhJ → putative hydratase YbhJ
RA 1,905,761 G→A G25D (GGT→GAT)  mntP → Mn(2(+)) exporter
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 3,216,473 A→G Y7H (TAC→CAC)  nfeF ← NADPH‑dependent ferric‑chelate reductase
MC JC 3,216,779 53253 bp→GTC [nfeR]yhaC 50 genes
RA 3,436,697 G→A E60E (GAG→GAA trkA → NAD‑binding component of Trk potassium transporters
RA 3,449,779 G→A S35S (AGC→AGT rpsC ← 30S ribosomal subunit protein S3
RA 3,468,768 C→T W362* (TGG→TGA chiA ← endochitinase
RA 3,474,425 T→C K43R (AAA→AGA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,523,800 C→G Y310* (TAC→TAG mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,725,393 G→A intergenic (‑75/+20) glyQ ← / ← ysaB glycine‑‑tRNA ligase subunit alpha/putative lipoprotein YsaB
RA 3,725,582 C→A C44F (TGC→TTC)  ysaB ← putative lipoprotein YsaB
MC JC 3,725,590 46159 bp→ATCG [ysaB]yibI 41 genes
RA 4,123,930 A→T V176E (GTA→GAA)  cytR ← DNA‑binding transcriptional repressor CytR
RA 4,174,041 A→T E320D (GAA→GAT birA → DNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA
RA 4,279,012 T→C D178D (GAT→GAC ghxP → guanine/hypoxanthine transporter GhxP
RA 4,279,031 A→G I185V (ATT→GTT)  ghxP → guanine/hypoxanthine transporter GhxP
MC JC 4,279,050 Δ82,373 bp [ghxP][cadC] 72 genes
RA 4,362,045 T→C intergenic (‑111/+506) cadC ← / ← pheU DNA‑binding transcriptional activator CadC/tRNA‑Phe
MC JC 4,488,473 Δ111,735 bp yjgRyjjA 100 genes
RA 4,600,215 +C intergenic (‑26/+23) yjjA ← / ← dnaC DUF2501 domain‑containing protein YjjA/DNA replication protein DnaC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423802–3424234 3424456–3424238 5–655 18 [15] [17] 19 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =29 (0.490)23 (0.410) 23/458 1.7 48.9% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 21 (0.380)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780527 (0.450)29 (0.520) 27/458 1.2 55.6% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 21 (0.380)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994980 (0.000)39 (0.670) 35/482 0.8 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein