BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,558,907	T→A	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,558,907	0	T	A	93.6%	318.5 / ‑5.2	112	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand): ref base T (0/7); new base A (53/50); total (55/57)
Fisher's exact test for biased strand distribution p-value = 1.32e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 115 total aligned reads displayed.

GCTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCT  >  NC_000913/4558660‑4559149

gctACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑Cattt                                                                                                                                                                                                                                                  >  2:201381/1‑251 (MQ=255)

gctACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑Cattt                                                                                                                                                                                                                                                  >  1:100140/1‑251 (MQ=255)

   aCCGTTGCCGTCGGAGCTGAGGGTGACGCTTGCCAGCGGAAGTCCCGCCTGAACGGCGCGGGCAATGCCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑Catttt                                                                                                                                                                                                                                                 <  2:626554/249‑1 (MQ=255)

      gTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGt                                                                                                                                                                                                                                            <  1:621405/251‑1 (MQ=255)

       ttGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTc                                                                                                                                                                                                                                           >  1:251382/1‑251 (MQ=255)

         gCCGTCGGAGCTGAGGGTGACGCGTGCCCGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGc                                                                                                                                                                                                                                         >  1:246829/1‑251 (MQ=255)

           cGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAACTAGGCTGTAACGC‑CATTTTACTGTCGccc                                                                                                                                                                                                                                       >  2:148483/1‑251 (MQ=255)

                 aGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGATCGTCAATGCTGCTGGTGATATCGAGGGTGCCGCCTTTGCGAGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCTCATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTATCGC‑CATTTTACTGTCGcccttcggg                                                                                                                                                                                                                                 >  2:681607/1‑245 (MQ=255)

                        ggTGACGCGTGCCAGCGGATGACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACgg                                                                                                                                                                                                                          <  1:65676/251‑1 (MQ=255)

                         gTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACggg                                                                                                                                                                                                                         >  2:53974/1‑250 (MQ=255)

                           gACGGGTGCCAGCGTAAGACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAAGAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACTCGGTg                                                                                                                                                                                                                        <  2:605246/250‑1 (MQ=255)

                            aCGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGAc                                                                                                                                                                                                                      >  1:311196/1‑251 (MQ=255)

                                  gCCAGCGGAATCCCCGCCTGAACGGCGCGGGCAATACCTCCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg                                                                                                                                                                                                                <  2:167989/251‑1 (MQ=255)

                                  gCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg                                                                                                                                                                                                                <  1:129207/251‑1 (MQ=255)

                                        ggAATACCCGCCTGAACGGCGAGGGCAATACCTTCGGCAGCGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCCAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCCGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTaac                                                                                                                                                                                                          >  2:636931/1‑249 (MQ=255)

                                           aTACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc                                                                                                                                                                                                       >  1:555488/1‑251 (MQ=255)

                                               ccGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGc                                                                                                                                                                                                   <  2:573668/251‑1 (MQ=255)

                                                      aaCGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGc                                                                                                                                                                                            >  1:527759/1‑251 (MQ=255)

                                                         ggagcgGGCAATACCTTCGGCAGGGGCGAACGGTTCGTCAATGCTGCTGGAGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGACATAACTAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACTCCAGGTTTACCGCCGAGCAAACCgaca                                                                                                                                                                                          >  2:500308/4‑247 (MQ=255)

                                                                gCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCggg                                                                                                                                                                                  >  2:76402/1‑250 (MQ=255)

                                                                 cAATACCTTCGGCAGGGGGGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGAt                                                                                                                                                                                <  2:285637/251‑1 (MQ=255)

                                                                  aaTACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGAtt                                                                                                                                                                               >  2:229495/1‑251 (MQ=255)

                                                                   aTACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTc                                                                                                                                                                              <  1:529013/251‑1 (MQ=255)

                                                                           ggCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGAGGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCa                                                                                                                                                                      <  2:251382/251‑1 (MQ=255)

                                                                            gCAGGGTCGTCCGGTGCGGCAATGCTGCTGGTGATATCGAAGGTTCGGCCTTTGCTCTCGAACTCAAGCGCTTGTTCAATCAATGGTGCGTTGCGGGTAAGGTGGGT‑CGGCAGCAGTTTGCTGAGCGGCTCAGCGCAGTTTTCCAGCTGGTAATAAATAGCCTG‑AACCCGCATTTTACTGTCGCCCATGTGAAACACGGTGACGCCAGGTTTACCGCCGAGCAATCC‑CAAAGCGCGGGATGCTGCCGCCat                                                                                                                                                                     <  2:298719/251‑1 (MQ=255)

                                                                            gCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCat                                                                                                                                                                     <  1:230805/251‑1 (MQ=255)

                                                                             cAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCCGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGACAGGTTTACCGCCGACCAAACCGCCAC‑CGCGGGATTCTgccgcaat                                                                                                                                                                     >  2:539480/1‑247 (MQ=255)

                                                                                gggCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTCCGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTg                                                                                                                                                                 >  2:458138/1‑251 (MQ=255)

                                                                                    gcccGGTTCGTAAAGGCTGCGGGTGATATCGAGGGTCCCGCCTTTGCGCGCGAACTCCAGCGCCGGCTAAAACAAGGGTCCGTTGCGGTTAACGGGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGAAACGC‑CATTTTACGGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCa                                                                                                                                                             <  2:70986/249‑1 (MQ=255)

                                                                                    gACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGACTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGcc                                                                                                                                                              >  1:644998/1‑250 (MQ=255)

                                                                                    gACCGGGTCGTCCATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGcc                                                                                                                                                              >  1:521660/1‑250 (MQ=255)

                                                                                     aCCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGcc                                                                                                                                                              >  1:590278/1‑249 (MQ=255)

                                                                                     aCCGGTTCGACAATGCGGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGATACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAACTAGGCTGTAGCGC‑CCTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGGTTACCGCCGAGCAAACAGCCAA‑CGCGGGGTTCTGCCGCCATATTGGcc                                                                                                                                                              >  2:557670/1‑249 (MQ=255)

                                                                                       cGGTTCGTCAATGCTGCTGTGGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGGGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCCCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGATCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGt                                                                                                                                                          <  1:624538/251‑1 (MQ=255)

                                                                                            cGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCAGAGCAAACCGCCAA‑CGCGGGAGTCTGCCGCCATATTGGCCAGGTGATa                                                                                                                                                      >  2:693419/1‑250 (MQ=255)

                                                                                             gTCAATGATGCTGGTGATCTCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAACCAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGATTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAC‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGAtca                                                                                                                                                     >  2:629070/1‑248 (MQ=255)

                                                                                               cAATGCTGCTGGTGATATCGATGGTGGCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCTA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACg                                                                                                                                                  >  2:259183/1‑251 (MQ=255)

                                                                                               cAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCGGCGCCTGCTCAAACATCGGTACGTTGCGGTTAACGGGGTT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTGGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCTGAGCAAACCTCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAAc                                                                                                                                                   <  1:608129/250‑1 (MQ=255)

                                                                                                aaTGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGAGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGCGCAAACCGCCAA‑CGCGGGATTCTGCCGCCCTATTGGCCAGGTGATAAACGt                                                                                                                                                 >  2:632200/1‑251 (MQ=255)

                                                                                                      gcgggTGATCTGGATGGTGCCGCCTTGACGCGCTAACTCCCGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGGGGGT‑CGGCAGCAGCTTGCGGACCGGCACAGCGCATTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGCCGCCAGGTTTCCCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGt                                                                                                                                            <  2:378802/247‑1 (MQ=255)

                                                                                                         ggtgattccGATGGTGCCGCCTTTGCCCGCGAACGCCAGCCCCTGCTCAAACAGCGGTACGGTGCGTTTAGCGTGGGT‑CGGCAGCGGCTTGCTGATCGGCACATCGCGGTTTTCCAGCAGGTGATAAATGGGCTGTAACGC‑TTTTTTACGGTCGCCCTGGGGGACCAGGGTGACGCCAGGTTTCCCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATAGTGGCCAGGTGATAAACGTCCGGTGCg                                                                                                                                         <  2:435589/242‑1 (MQ=255)

                                                                                                         ggTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCgg                                                                                                                                        >  1:8962/1‑251 (MQ=255)

                                                                                                          ggtagttggaggggcccgccTTTGCGCGCGAACCCCAGCGCCTGCTCAACCACCGGTCCGTTGCGTTTAAGGTGGGT‑CGGCAGCATCTTGCTGATCGCCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CTTTTTACTGTCGCCCATGTGGAACACGGGGACGCCAGGTTTCCCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGc                                                                                                                                       <  2:507260/236‑1 (MQ=255)

                                                                                                            gATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGTTCATAAATAGGCTGTAACGC‑CTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTTCCGCCATATTGGCCAGGTGATAAGCTTCCGGGGCGGCAg                                                                                                                                     <  1:238786/251‑1 (MQ=255)

                                                                                                            aagttCGAGGGTGCCCCTTTTGCGCACGAACCCCGCCCCCTGCTCAAACAACTTTACTTTGCTGTTAACGGGGGT‑CGGCAGCAGCTTCCTGATCGGCACATCTCAGTTTTCCAGCTGGTCATAAATTGGCGATAACGC‑CATTGTACTGTCGCCCTGGGGGACCACGGGGTGACCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGTTTCTGCCGCCATATTGGCCAGGTGATAATCGTCCGGTGCGGCAg                                                                                                                                     <  2:451019/247‑1 (MQ=255)

                                                                                                             atatCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCTGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGc                                                                                                                                       >  1:451019/1‑248 (MQ=255)

                                                                                                                  gATGGTGCCGCGTTTGGGCGGGAAATCAAGCGCCTGCTCAAACAACGGTGCGTTGCGGTTAACGTGGGT‑CGGCAGCGGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTGACCGCCGAGCAGAGCGCCCA‑CGCGGGATTCTGCCGCCATATTGACCCGGTGATACACGCCCGGTGCGGCAGAACggg                                                                                                                               >  2:680980/1‑250 (MQ=255)

                                                                                                                  gATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACACCGGTACGTTGCGGTTAACGTGGCA‑CGGCAGCAGCTTGCTCATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGt                                                                                                                               >  1:676221/1‑251 (MQ=255)

                                                                                                                   ggggTGCCGCCTTGGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTGTTCCAGCAGGTCATAAAGAGGCTGTAACGC‑CATTTTACTGTCGCCCATGGGGAACAGGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTg                                                                                                                              <  2:504673/249‑1 (MQ=255)

                                                                                                                     ggTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGTTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGGCCGGTGCGGCAGAACGGTGAt                                                                                                                            <  1:232313/251‑1 (MQ=255)

                                                                                                                      gTGCCGCCTTGGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATc                                                                                                                           <  1:340092/251‑1 (MQ=255)

                                                                                                                       ccccgccTGTGCCCGAGACCTCCAGCTCCGGCTCAAACAGCGGTTCGTTGCGGTTAACGTGGGT‑CGGCAGCCGCTTGCTGATCGGCACATCGCAGTTTTCCACCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCTATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAACCCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCa                                                                                                                          <  2:10204/249‑1 (MQ=255)

                                                                                                                        gccgccTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGA‑CGGCCGCCGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTCACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGCGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTACGGTGCGGCAGAACGGTGATc                                                                                                                           >  1:294424/1‑249 (MQ=255)

                                                                                                                         ccgccTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGGGCGGCAGAACGGTGATc                                                                                                                           >  2:146402/1‑248 (MQ=255)

                                                                                                                           gccTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGt                                                                                                                                                                                                               >  1:614510/1‑163 (MQ=255)

                                                                                                                           gccTTTGCCCGAGAAATCCCGAGCCTGCTCACCCAACGGTACGCCGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCCCAGTTTTCCAGCCGGTCATCAATAGGCTGTACCGC‑CCTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCCAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTgctccg                                                                                                                         >  2:55550/1‑243 (MQ=255)

                                                                                                                              tttGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATgg                                                                                                                   >  1:276149/1‑251 (MQ=255)

                                                                                                                                   gctcgAACTCCCGCGCCTGCCCAAACACCGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTTCTGATCGGCACATCGCAGTTTTCTAGTAGGTCAAAAATAGGCTGTAACGC‑CATTTTACTTTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCTCCGGGTAAACCTCCAT‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCAt                                                                                                              <  1:85844/248‑1 (MQ=255)

                                                                                                                                    cgcgAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCAtt                                                                                                             >  1:349321/1‑251 (MQ=255)

                                                                                                                                     gcgAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCAttt                                                                                                            >  1:506978/1‑251 (MQ=255)

                                                                                                                                      cgacccccggcgcCTGCCCAAACAACGGTCCGTTGCGGTTAAGGTGGGG‑CGGCACCAGCTTGCTGATGGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCAGGTGGAACACGGTGAGGCCAGGTTTCCCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTc                                                                                                           <  2:55015/242‑1 (MQ=255)

                                                                                                                                      cgaccTCCAGCGCCTGCTAAAACAACGGTACGGTGCGGTGAACGTGGGT‑CGGCAGCAGCTTGCGTATCGGCACATCGCAGTTTTCCAGCAGGTCAAAAATGGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGGCGCCAGGTTTACCGCCGAGCAACCCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTc                                                                                                           <  2:508135/247‑1 (MQ=255)

                                                                                                                                       aaaCTCCAGCGCCTGCTCAACCAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCa                                                                                                          <  1:491776/250‑1 (MQ=255)

                                                                                                                                        aaCTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCAc                                                                                                         >  2:221665/1‑251 (MQ=255)

                                                                                                                                         aCTCCAGCGCCTGCTAAAACAGCGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTAATAAATAGGCTGAAACGC‑CATTTTACTGTCGCCCATGTGGAACAGGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCGGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACg                                                                                                        <  2:152038/251‑1 (MQ=255)

                                                                                                                                          ccccAGCGCCGGCTCAAACAGCGGTACGTGGCGGTTAACG‑GGGTGCGGCAGCGGCTTGCTGAGCGGCACATCTCAGTTTTCCATCAGGTCAAAAATAGGCTGTAACGC‑CATTTTACTTTCGCCGATGTGGAACACGGTGACGCCAGGTTTCCCGCCGAGCAAACCGCCAA‑CGCGGGATGCTGCCGCCATATTGGCCAGGTGATAAACGGCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCAGTTCACGc                                                                                                       <  2:259074/249‑1 (MQ=255)

                                                                                                                                            ccAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGc                                                                                                       <  1:229495/249‑1 (MQ=255)

                                                                                                                                                 gcCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATcac                                                                                                <  1:180612/251‑1 (MQ=255)

                                                                                                                                                 gcCTGCTCAAACAACGGTACGTTGCGGTTAACGGGGGT‑CGGCAGCAGCTTGCTGAGCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATcac                                                                                                <  1:254336/251‑1 (MQ=255)

                                                                                                                                                      cccAAACAACGGTAGGTGGCGTGTACGGTGGGT‑CGGCAGTAGCTTGCGGATCGGCACATCGCAGTTTGCCAGCAGGTCATAAAGAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGACCAGGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcgat                                                                                           <  2:62672/249‑1 (MQ=255)

                                                                                                                                                             aacGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGGTCGATAAt                                                                                    >  2:479385/1‑251 (MQ=255)

                                                                                                                                                                       tGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTCCTGTCGCCCATGTGGAACCCGGTGACGCCGGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTc                                                                           <  1:145490/250‑1 (MQ=255)

                                                                                                                                                                         cGGTTAACGGGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCttt                                                                        <  1:148483/251‑1 (MQ=255)

                                                                                                                                                                          ggTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCACATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCtttt                                                                       >  1:624512/1‑251 (MQ=255)

                                                                                                                                                                             tAACGTGGGG‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCGTGTGGAACACGGTGACGCCGGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATGCTGCCGCCAGATTGGTCATGTGATAAACGTCTGGTGCGGCAGAACTGTGATTAGATATGTCTCATTGCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTc                                                                     <  1:88266/250‑1 (MQ=255)

                                                                                                                                                                                            agcagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGCGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAAt                                                      >  1:620610/1‑251 (MQ=255)

                                                                                                                                                                                                  ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCCGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCg                                                >  1:459220/1‑251 (MQ=255)

                                                                                                                                                                                                   tgcggATCGGCCCATCGCAGTTTTCCCGCGGGTCATAAATAGGCTGAAACGC‑CATTTTACTTGCCCCCTTGTGGAACCGGGGGACGCCAGTTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGc                                                 <  2:512675/245‑1 (MQ=255)

                                                                                                                                                                                                    gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCggg                                              >  1:565763/1‑251 (MQ=255)

                                                                                                                                                                                                      tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGa                                             >  2:116011/1‑250 (MQ=255)

                                                                                                                                                                                                       gATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGa                                             >  2:376333/1‑249 (MQ=255)

                                                                                                                                                                                                       gATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAg                                           <  1:221665/251‑1 (MQ=255)

                                                                                                                                                                                                        aTCGGCACATCGCAGTTTTCCAGCAGGTCAAAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAgg                                          <  1:570862/251‑1 (MQ=255)

                                                                                                                                                                                                         tCGGCACATCGCAGTTTTCCAGCAGTTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCCCGTCTTTTTCCACGGAACCCGCAATGGTGCGGGAAggg                                         >  2:15516/1‑251 (MQ=255)

                                                                                                                                                                                                          cGGCACCTCGCTGTTTTCCAGCATGTCATAAAGAGGTTTTAACTC‑CATTTTACTGTCGCCCTTGTGGACCACGGTGTCCCCAGGTTTACCGCCGACCAAACCCCCAA‑CGCGGGATTCTGCCGTCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGa                                        <  1:1037/251‑1 (MQ=255)

                                                                                                                                                                                                           ggcccaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGAc                                       <  1:504168/247‑1 (MQ=255)

                                                                                                                                                                                                                 tCGCAGTTTTCCACCAGGCCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCTTGTGGCACACGGTGACGCCAGGTTTAACGCCCCGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACCTCCGGTGAGGCAGAACGGTGCTCAGAGATGGCGCATTTCACGCCAATCCCACGTTCGATAATCGCCACGTCTTTTTCCACGGAACCCCTAATGGTCGGGGAAGGGACATGAt                                  >  2:638798/1‑250 (MQ=255)

                                                                                                                                                                                                                   gCAGTTTTCCAGCAGTCCATAAATAGGCTGTAACGC‑CATTTTACTGTGGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCGGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAACGGTGCGGGAAGGGACATGATAAg                               <  2:283965/251‑1 (MQ=255)

                                                                                                                                                                                                                      gTTTTCCAGCCGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAgcgc                            >  2:642596/1‑251 (MQ=255)

                                                                                                                                                                                                                        tttCCAGCAGGGACTAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGCAGGGACATGATAAACGCCg                          >  2:692642/1‑251 (MQ=255)

                                                                                                                                                                                                                         ttCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCgg                         >  1:433354/1‑251 (MQ=255)

                                                                                                                                                                                                                         ttCCAGCAGGTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCgg                         >  1:129037/1‑251 (MQ=255)

                                                                                                                                                                                                                             accagGTCATAAATGGGCTGTAACGC‑ATTTTGACTGTCGCCCATGTGGAACACGGTGACGCCGGGTTTACCCCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAg                     <  1:413871/249‑1 (MQ=255)

                                                                                                                                                                                                                                gggTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGGCGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCAt                  <  1:240809/250‑1 (MQ=255)

                                                                                                                                                                                                                                gggTCATAAATAGGCTGTAACGC‑CATTTTACTGTCGCCCATGGGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CTGGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCAt                  <  1:93015/250‑1 (MQ=255)

                                                                                                                                                                                                                                agGTCATAAATAGGCTTTAACGC‑CATTTTACTGGCGCCCATGGGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCAt                  <  1:458138/251‑1 (MQ=255)

                                                                                                                                                                                                                                      aaaaTGGGCTGTAACGC‑CTTTTTACGGTCGCCCAGGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGgc            <  1:510770/250‑1 (MQ=255)

                                                                                                                                                                                                                                       aaaTAGGCTTTATCGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCGGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGgcg           <  1:367506/251‑1 (MQ=255)

                                                                                                                                                                                                                                                gTAACGC‑CATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCct  >  1:80783/1‑251 (MQ=255)

                                                                                                                                                                                                                                                gTAACGC‑CATTTTACGGTCGCCTATGTGGACCACGGTGCCGCCGGGTTCACCGCCGAGCAAACCGCCAC‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCct  <  2:527759/251‑1 (MQ=255)

GCTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGC‑CTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAA‑CGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCT  >  NC_000913/4558660‑4559149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`