Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	149.8 / NA	40	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/20);  total (20/20)

ATGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCC  >  NC_000913/1652087‑1652571                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  aTGGTGTGTTTCTGGTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAAGTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGTAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCGCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCagag                                                                                                                                                                                                                                             <  2:78924/251‑1 (MQ=255)    gtgtgtTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGt                                                                                                                                                                                                                                          <  2:499228/251‑1 (MQ=255)          ttCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTTACCTCAGAGAGTATGGc                                                                                                                                                                                                                                     >  1:503656/1‑250 (MQ=255)              gTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGcag                                                                                                                                                                                                                                   <  1:464487/248‑1 (MQ=255)                    aCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGATTATGGCAGAACAGaaaa                                                                                                                                                                                                                          <  2:276581/251‑1 (MQ=255)                    aCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGaaa                                                                                                                                                                                                                           <  1:874/250‑1 (MQ=255)                     cTGGAGTTCGCACTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACAGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAAACGGGTCCTTTTTATTATTAAGCCAGCAAACGACGCTCTCCGGGGTCAGATGACATTAACGAGACTGAGCAAAGAGAATAT‑CATTGATTTTCACCTCAAAGAGTATGGcagcaccaaaaa                                                                                                                                                                                                                          >  2:336284/1‑241 (MQ=255)                        gAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAACTTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGGCCTTCATCTTATTAAGCCCCCGATAGAAGCTCTCCCGGGTCAGATGACATTACCGAGACTGAGCCCAGAGAAACT‑CATTGATGTTCACCTCCGAGAGTATGGGCGAACAGaaaacaa                                                                                                                                                                                                                       >  1:286894/1‑247 (MQ=255)                            tCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTTATGTTCACCTCAGAGAGTATGGCATAACAGAAAAACaaaaa                                                                                                                                                                                                                   <  1:138585/250‑1 (MQ=255)                                    gTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑TATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCttt                                                                                                                                                                                                          <  2:138913/251‑1 (MQ=255)                                      gAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGt                                                                                                                                                                                                        <  2:503656/251‑1 (MQ=255)                                                     ggATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAgt                                                                                                                                                                                         <  1:169834/251‑1 (MQ=255)                                                     ggATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAg                                                                                                                                                                                          <  1:379161/250‑1 (MQ=255)                                                      gATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATTGGGTAATTCATCCTATTAAGCCAACAATACACGCTCTCCGGGGTCAGATGAAATTAACGAGACCGAGCAAAGAGCCTAT‑CATTAATGTTCACCTCACAGAGTATGGCAGGACCGAAAAACAAAAATGCACCTTTGTTTTTCTACCTGaaacg                                                                                                                                                                                        >  1:77254/1‑248 (MQ=255)                                                                   aGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑Ta                                                                                                                                                                           <  1:11457/250‑1 (MQ=255)                                                                        tGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGTAA‑CACACGGGGCATTTATCTTTTTAAGCCAGAAATCGAACATCTCCGGAGTCAGATGACATTAACGAGACTGAACAAAAAGCATAT‑CCTTTATGTTCACCTCAGAGAGCATGGCAGAACAGAAAAAAAAAAATTCCACTTTGGTTTTCAACCTGAAGTGGCAGCgcgggaaaaaaa                                                                                                                                                                       >  2:285701/1‑239 (MQ=255)                                                                            aaaTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAgt                                                                                                                                                                                         <  1:186239/228‑1 (MQ=255)                                                                            aaaTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAA‑CAAACGGGGCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAAAGAGACTGAGCAAAAAGCATAT‑CATTGATGTTCACCTCAGAGAGGATGGCAGAACAGAAAAACAAAAATGAACCTTTGTTTTTCAAACTGAAgaagaacggaaaagagtcgtgtagg                                                                                                                                                                  >  2:186239/1‑227 (MQ=255)                                                                                ggAACAATGATGATCAGGAGCAATTTAGCAAAAGACCGTTTCACGGTACCAACAACACAGGCGGCAAC‑CAAACGGGTCATTCCTCTTCTTAAGCCAGCAATCCACGCCCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAAAGACTAT‑CATTGATGTTCACCTCCGAGAGGTTGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGCGTCAGCGAGAG‑TAAAAAATTATGGTg                                                                                                                                                              >  1:588452/1‑250 (MQ=255)                                                                                  aaCAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAA‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCCGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAAAAAAATGCACCTTTGTTTTTCAACCTGAAGGGTCAGCGAGAG‑TAAAAAATTATGGTTAcc                                                                                                                                                           >  2:144973/1‑251 (MQ=255)                                                                                               aGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAA‑CAATCGGGTCATTCATATTATTAAGCCAGCAATCGACGCTCCCCGGAGTAAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAAAAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTg                                                                                                                                               >  1:59712/1‑250 (MQ=255)                                                                                                ggAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGATACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTGAc                                                                                                                                             <  1:195818/251‑1 (MQ=255)                                                                                                    aaaTTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGCAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGGTTTTCAACCTGAAGAGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTGACTCa                                                                                                                                          >  1:565713/1‑250 (MQ=255)                                                                                                        ttAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CCAACGCGTCATTCATCTTATTAAGCCAGCCATCGAAGCTCTACGGAGTCAGATGACATTAACACGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTGTGGCAGAACAGAAAAACACAAATGCCCCTTTGTTTTTCAACCTGAAGGGTCAGCGCGAG‑TAAAAAATTATGGTGACCATTTTTCCGTTGACTCAATaa                                                                                                                                      >  1:87075/1‑250 (MQ=255)                                                                                                               aaGACCGTTTCACGGTACCAAAAACACAGGCGGGTAC‑CAATCGGGGCATTCACCTTATTAAACCCGCCATCGCAGCACTCCGGGGGCAGATGAAATTAAAGAGACTGGGCAAAACGCATAT‑CATTGTTGTTCACCTCAGAGAGTATGGGCGAACAAAAAAAAAACAATGCCCCCTTGTTTTTTAACCTGCAGTGTAAGCGAGAG‑GAAAAAATTTTGGTGAACAtttttacggtgatgccaaaatgcaga                                                                                                                                >  2:235436/1‑227 (MQ=255)                                                                                                                       ttCACGGTACCCACAACACAGGCGG‑AGCACAATGGGGTCTTTCATCTTATTAAGCCACCAATCGGCGCCCTCCGGGGTCAGAAGACATTAACGCGACTGAGGACAGAGCATTTACTTTG‑TGTTTAACTCAGAGAGTATGGCAGAAAAGAAAAAAAAAAATACACCCTTTTTTTTCAACCTGGAGTGTCAGCGAGAG‑GAAAAAATTATGGTGACCATTTTACCGTTGACTTAATTAGGCAGATGgggggggc                                                                                                                      >  2:344614/1‑247 (MQ=255)                                                                                                                       ttCACGGTACCAAAAACACAGGCGGGAAC‑CAGTCGGGGCCTTCTTCTTTTTAAACCAACAACAGGAGCTCTCCGGGGGCCGGTGGAATTAAAGAGCATGAGCAAAACGCATAT‑CCTTTACGGTCAACTCCGGGAGTATGGCCGAACAGAAAAAAAAAAATGCACCATTGTTTTTCAACCTGAAGAGTCAACGGGAGACAAAAAATTATGGGGAC‑AATTTACCGTTggcttggtgatagttagggctta                                                                                                                         >  2:456969/1‑226 (MQ=255)                                                                                                                                                 aaC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGcc                                                                                            >  1:644530/1‑251 (MQ=255)                                                                                                                                                  aC‑CAATCGGGTCAGTCATCTTATTAAGCCCGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTGTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGGGACCATTTTACCGGTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCa                                                                                           <  2:286894/251‑1 (MQ=255)                                                                                                                                                      aaTCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCg                                                                                        <  1:144973/251‑1 (MQ=255)                                                                                                                                                       aTCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATAGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCg                                                                                        >  2:585076/1‑250 (MQ=255)                                                                                                                                                                                   aaTCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATg                                                           >  2:663229/1‑251 (MQ=255)                                                                                                                                                                                       gACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCACTTTACCGTGGACTCAATAAGGCAGATGTGTGACACAGCGATAAAGCGTGCCGGACGCCGCCTTCGAAAATCATATCAGTCGAGACATACTTATGCctg                                                       <  2:637389/251‑1 (MQ=255)                                                                                                                                                                                            tctcCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGt                                                  >  2:217837/1‑251 (MQ=255)                                                                                                                                                                                               ccGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTc                                                 <  2:644530/249‑1 (MQ=255)                                                                                                                                                                                                       aGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATGTATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTGACACAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCt                                       <  1:693595/251‑1 (MQ=255)                                                                                                                                                                                                        gATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTg                                      <  1:469562/251‑1 (MQ=255)                                                                                                                                                                                                                 aaCGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAAcc                             >  1:331136/1‑251 (MQ=255)                                                                                                                                                                                                                        cTGAGCAAAGAGCATAT‑CATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCAtt                      >  1:137090/1‑251 (MQ=255)                                                                                                                                                                                                                                              gATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTAGGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGcc  <  2:565713/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  ATGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATAT‑CATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAG‑TAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCC  >  NC_000913/1652087‑1652571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑