Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	218.5 / NA	59	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (32/27);  total (32/27)

CTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCG  >  NC_000913/3560207‑3560688                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          cttcttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTAATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgc                                                                                                                                                                                                                                          >  2:70036/1‑249 (MQ=255)        tACCATCAACTTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGACGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTA‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTTGAATGCAGCGCCCCCCCGCCATGATGGcgcaggctcc                                                                                                                                                                                                                                   >  1:10533/1‑242 (MQ=255)         cccatcaccgtttaagaaaCGTTGAGATGGTTGGGGAAAGTGGGCAATTTGCTGTGATTGAGCAGTGCGGGCGCTACCGTTTCGGGGGTGGTGCCATTACCGATAAACAGCGTCGGGCCTTGGGGGATTGGCTCC‑GCCACTTTG‑CGGGCGATGCGCGCTTTTTCTTCGTTCGGGGTGGCCTTGCGATCTTGCCACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAg                                                                                                                                                                                                                                  <  1:609061/234‑1 (MQ=255)            aTCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGtt                                                                                                                                                                                                                               >  2:260823/1‑251 (MQ=255)                  gtgtTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGAGATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGCAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTc                                                                                                                                                                                                                         >  1:224698/1‑251 (MQ=255)                       aGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGcc                                                                                                                                                                                                                     >  1:77782/1‑250 (MQ=255)                             gTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAt                                                                                                                                                                                                              >  1:167529/1‑251 (MQ=255)                             gTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAt                                                                                                                                                                                                              >  1:455703/1‑251 (MQ=255)                                 aaaTTGTGGGTGACAATGCGCAATTTGTTGTGATTGAGCGGTGCGTGCGCTCCCGCTCCCGGCTGGGTGCCGATATCGATAAACAGCGTCGAGCCATGGGGGATTGGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                                                                                                                                                                          <  1:126024/249‑1 (MQ=255)                                   aTTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGt                                                                                                                                                                                                        >  2:546614/1‑251 (MQ=255)                                          gTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggcg                                                                                                                                                                                                 >  1:117885/1‑251 (MQ=255)                                             aCAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTCCCGCTTCGGGCGGGGTGCCGATATCGATAACAAGCGTCGAGCCTTTGGGGATTTGCCCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                                                                                                                              <  1:260823/251‑1 (MQ=255)                                                aTGCGCCAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt                                                                                                                                                                                           >  1:216222/1‑251 (MQ=255)                                                      aaaTTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg                                                                                                                                                                                     >  1:383052/1‑251 (MQ=255)                                                              gtgATGGAGCAGTGCGTGCGCTCCCGCTTCCGGCGGGGTGCCGATATCGATAAACGGCTGCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                                                                                                                              <  1:475344/250‑1 (MQ=255)                                                                        aGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc                                                                                                                                                                   >  1:384043/1‑251 (MQ=255)                                                                        aGTGCGTGCGCTACCGCTTACGGCGTGGTGCCGCTATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc                                                                                                                                                                   >  2:96379/1‑251 (MQ=255)                                                                                 gcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc                                                                                                                                                          >  2:132797/1‑251 (MQ=255)                                                                                    cccgtttCCGGGGGGGTGCCGATATCGATAAACGGCGTCGCGCCATGGGGGTTTTGCCCC‑GCCACTTGG‑CGGGCGATGCGCTCTTTTTCTCCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAGAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                       <  1:249244/246‑1 (MQ=255)                                                                                       gCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                                                                                                                                    >  1:440642/1‑251 (MQ=255)                                                                                            cGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                                                                                                                               >  1:230300/1‑219 (MQ=255)                                                                                             ggcgggggccatatatgaaaaaaCAGCGCCGA‑CCCTTGGGGATTTGCCCCCGCCACTTTG‑CGGGGGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCCCCCCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGAc                                                                                                                                              <  2:167529/231‑1 (MQ=255)                                                                                               cGTGGTGCCGATAGCGATAAACAGCGTAGAGCCATTGGGGTTTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGGTCACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTATGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTCCCTGCTCTTCGGTACTGACAt                                                                                                                                            >  2:58145/1‑251 (MQ=255)                                                                                                  ggtgCCGATCTCGATAAACAGCGTCGAGACATTGGAGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCAAGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCCTGATGGCGCAGGATCAGGTTTTGCTCCGCCACCTCATTGAGGTAGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCTTTCGGGACTGACATacc                                                                                                                                         >  2:674952/1‑249 (MQ=255)                                                                                                    tgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGCTGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc                                                                                                                                       >  1:353580/1‑251 (MQ=255)                                                                                                     gCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCCGCTCTTCGGTACGTACATAAcccc                                                                                                                                      >  1:166583/1‑250 (MQ=255)                                                                                                      ccaatagcgaaaaccAGCGTCGAGCAATTGGGGATTGGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTCGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                                                                                                                                     <  1:226681/237‑1 (MQ=255)                                                                                                       gacgtgggctctcccgatcggggCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc                                                                                                                                    <  1:387434/229‑1 (MQ=255)                                                                                                           tacaaTAAACAGCGTGGAGCCATGGGGGTTTTGCTCC‑GCCATTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCGCGGCGTGTTAACCGAACTGGAAGGCTGCGCCCCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGATCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt                                                                                                                                 <  1:445021/246‑1 (MQ=255)                                                                                                                  aaCAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                                                                                                                               >  1:323151/1‑229 (MQ=255)                                                                                                                   acggCGTCGGGCCATGGGGTTTTTGCCCG‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTGCGGTCTGGGGTGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGt                                                                                                                        <  1:569760/248‑1 (MQ=255)                                                                                                                     ccctggggccctgtGGGGTTTTGCCCC‑GCC‑CTTTGGCGGGCGAGGCCCTCTTTTTCTTCGTTCGGGGTGGCCTTGCACTCGTGCCACGGCGTGTTACCCGACCGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTc                                                                                                                      <  2:570396/238‑1 (MQ=255)                                                                                                                           gagctgttGGGGTTTTCCTCC‑GCCTCTTGG‑CGGGCGATGCGCTCTGTTGTGTCGGTCTGGGTGGCCTTGCGTTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCCCCGCCCCGCCATGATGGCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat                                                                                                                <  1:300407/245‑1 (MQ=255)                                                                                                                           gAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc                                                                                                                                    >  2:387434/1‑231 (MQ=255)                                                                                                                           gAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat                                                                                                                >  1:159617/1‑251 (MQ=255)                                                                                                                            aGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat                                                                                                                >  1:160250/1‑250 (MQ=255)                                                                                                                                       ttttGCTCC‑GCCACTTTG‑GGGGCGATGCGCTCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGAc                                                                                                     <  2:455703/249‑1 (MQ=255)                                                                                                                                           gCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTCAGTGGCCTTGCGATCGTGACACGCCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCCTGGTGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGGTTAACCAGTTCGATAATACCGCTGTGACgctgg                                                                                                >  1:97872/1‑247 (MQ=255)                                                                                                                                             ccc‑GCCCCTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTgtgt                                                                                              <  1:127967/250‑1 (MQ=255)                                                                                                                                              cc‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtt                                                                                             >  2:260165/1‑251 (MQ=255)                                                                                                                                                 gCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttg                                                                                           >  1:367878/1‑251 (MQ=255)                                                                                                                                                  ccACTTGG‑CGGGCGATGCGCTCTTTTGCTTCGGTCTGGGTGGCCTTGCGAGCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgt                                                                                          <  1:70036/251‑1 (MQ=255)                                                                                                                                                   ccctttggggggggATGCGCCTTTTTTCTTCGGTCGGGGTGGCCTGGCGACCGTGCCCCGGGGTGTAAACCGAACTGGAGGGCGGCCCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgt                                                                                          <  2:216222/238‑1 (MQ=255)                                                                                                                                                           ggggcggggcgcTTTTTTTTTTCGGTCGGGGTGGCCTTGCGCTCGGGCCACGGCGTGTTAACCGAACTGGAGGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTta                                                                                  <  2:440642/243‑1 (MQ=255)                                                                                                                                                                    cgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATGTATAAATCCCt                                                                         <  1:595568/251‑1 (MQ=255)                                                                                                                                                                           tttCTTCGGTCTGGGAGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAACCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGAAattt                                                                  >  2:477819/1‑250 (MQ=255)                                                                                                                                                                             tcttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAattat                                                                 <  1:174082/250‑1 (MQ=255)                                                                                                                                                                               ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAtttt                                                              >  1:388329/1‑251 (MQ=255)                                                                                                                                                                                tcGGTCTGGGTGGCCTTGCGATCGTGCCCCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAattatt                                                                <  1:96379/248‑1 (MQ=255)                                                                                                                                                                                          tGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCgcgc                                                   >  2:293024/1‑251 (MQ=255)                                                                                                                                                                                                tGCGCTCGTGCCCGGGCGTGTTAACCGAACGGGAAGCCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg                                               <  2:159617/249‑1 (MQ=255)                                                                                                                                                                                                     ccgcgCCACGGGGTGTTAACCGAACTGAAGGGCGGCGCGGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                                        <  2:189345/247‑1 (MQ=255)                                                                                                                                                                                                         gCCACGGCGTGTTAACCGAACTGGAAGGAAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGCAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGATCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaac                                    >  1:582752/1‑251 (MQ=255)                                                                                                                                                                                                         gCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa                                     <  1:461803/250‑1 (MQ=255)                                                                                                                                                                                                          ccACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaac                                    >  1:648175/1‑250 (MQ=255)                                                                                                                                                                                                            aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCTATGATGGCGCAGGCTCAGGTTTTGCTCAGCCAGCTCATTGCGGTCGCGGCGCATAGTATGCGGGCTGACGGAGAACTGCTCTACCAGATATTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaacaa                                  >  1:415179/1‑250 (MQ=255)                                                                                                                                                                                                                               ggAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACg              <  1:132797/251‑1 (MQ=255)                                                                                                                                                                                                                                 aaggacgccctccACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGTCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTGCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATGATTTTCGTTTTCGCGCATTGAGCGAACCAACAAAATCCATCGCTAAACCCACGGc            <  2:41977/239‑1 (MQ=255)                                                                                                                                                                                                                                        ggccgcTCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTGATAAATCCCTGGAATTAGTTTCGTGTTCGTGGATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCg     <  1:653394/250‑1 (MQ=255)                                                                                                                                                                                                                                          ccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACTGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc   <  1:109022/251‑1 (MQ=255)                                                                                                                                                                                                                                            acACCGCCATGATGGCGCCGGATCAGGTTTTGTCCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGACATGCTCTACCCGCTCTTCGGTACTGCCATAACCCTGCTGTTTAACCAGTTCGATCATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTCTTTTCGTTTTCGCGCCTTGAGCGAATCAACAAAAGCCATCGCTCAACCCACGGCTAACCCGGCg  >  2:129979/2‑250 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          CTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCC‑GCCACTTTG‑CGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCG  >  NC_000913/3560207‑3560688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑