breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 3,645 G→A W282* (TGG→TAG)  thrB → homoserine kinase
RA 71,170 T→C S262P (TCG→CCG)  araC → DNA‑binding transcriptional dual regulator AraC
RA 81,102 G→A S286L (TCG→TTG)  leuB ← 3‑isopropylmalate dehydrogenase
RA 154,182 C→T L415L (CTG→CTA htrE ← putative fimbrial usher protein HtrE
RA 156,068 A→G V45A (GTA→GCA)  yadV ← putative fimbrial chaperone YadV
RA 160,864 (C)6→7 coding (623/705 nt) sfsA ← sugar fermentation stimulation protein A
RA 210,002 Δ1 bp coding (324/2142 nt) ldcC → lysine decarboxylase 2
MC JC 255,892 Δ18,063 bp IS5‑mediated gpt[ykfC] 26 genes
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 292,019 C→T L174L (CTA→TTA)  yagJ → protein YagJ
RA 309,381 C→T D216N (GAT→AAT)  ecpB ← putative fimbrial chaperone EcpB
RA 309,985 T→A G14G (GGA→GGT ecpB ← putative fimbrial chaperone EcpB
RA 321,260 A→T intergenic (+179/‑348) rclR → / → ykgE DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
RA 410,666 C→T P175S (CCT→TCT)  mak → fructokinase
RA 421,027 C→T R14R (CGC→CGT proY → putative transporter ProY
RA 431,912 C→T W34* (TGG→TGA tsx ← nucleoside‑specific channel‑forming protein Tsx
RA 465,005 G→A V181V (GTC→GTT queC ← 7‑cyano‑7‑deazaguanine synthase
RA 472,849 A→G G84G (GGA→GGG glnK → nitrogen regulatory protein PII‑2
RA 495,622 C→T T168I (ACC→ATC)  htpG → chaperone protein HtpG
RA 495,988 A→G N290S (AAC→AGC)  htpG → chaperone protein HtpG
RA 534,742 G→A G276D (GGT→GAT)  gcl → glyoxylate carboligase
MC JC 575,786 Δ3,398 bp IS5‑mediated [nmpC]borD [nmpC], essD, rrrD, rzpD, rzoD, borD
RA 582,597 C→T intergenic (+500/‑278) tfaD → / → tfaX putative tail fiber assembly protein TfaD/protein TfaX
RA 694,941 G→A L5L (CTC→CTT miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 708,774 G→A A147A (GCG→GCA chiP → chitooligosaccharide outer membrane channel
RA 721,604 G→A R44C (CGT→TGT)  kdpE ← DNA‑binding transcriptional activator KdpE
RA 780,624 C→T noncoding (71/76 nt) lysT → tRNA‑Lys
RA 789,580 C→A E134* (GAA→TAA)  galK ← galactokinase
JC JC 820,914 IS5 (+) +4 bp coding (122‑125/714 nt) ybhM → Bax1‑I family protein YbhM
RA 822,527 C→T E405K (GAA→AAA)  clsB ← cardiolipin synthase B
RA 848,112 (A)5→6 intergenic (‑108/+296) glnH ← / ← dps L‑glutamine ABC transporter periplasmic binding protein/DNA protection during starvation protein
RA 883,396 C→T intergenic (‑8/‑277) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 908,169 C→T R38R (AGG→AGA ybjT ← putative NAD(P)‑binding protein YbjT
RA 1,038,477 2 bp→AA coding (738‑739/1545 nt) appC → cytochrome bd‑II subunit 1
JC JC 1,105,699 IS1 (+) +9 bp coding (286‑294/312 nt) ymdA → uncharacterized protein YmdA
RA 1,123,823 2 bp→AG coding (231‑232/648 nt) grxB ← reduced glutaredoxin 2
RA 1,147,792 T→A S58T (TCG→ACG)  plsX → putative phosphate acyltransferase
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,194,922 G→A T10T (ACC→ACT rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,201,548 A→C T18P (ACC→CCA)  ymfI → uncharacterized protein YmfI
RA 1,201,550 C→A T18P (ACC→CCA ymfI → uncharacterized protein YmfI
RA 1,253,002 C→T intergenic (+17/+83) dhaR → / ← ycgV DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
JC JC 1,290,542 IS1 (+) +9 bp coding (301‑309/1014 nt) rssB → regulator of RpoS
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,308,684 T→C intergenic (‑39/‑104) clsA ← / → yciY cardiolipin synthase A/uncharacterized protein YciY
RA 1,331,059 2 bp→AT coding (12‑13/2598 nt) topA → DNA topoisomerase 1
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,395,367 G→A R186C (CGT→TGT)  ynaI ← small conductance mechanosensitive channel YnaI
RA 1,405,758 G→A intergenic (‑7/‑221) mcaS ← / → smrA small regulatory RNA McaS/DNA endonuclease SmrA
MC JC 1,411,925 Δ23,060 bp [ttcA]ynaM 34 genes
RA 1,460,045 A→G Q331R (CAG→CGG)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase
RA 1,497,385 4 bp→CAA coding (530‑533/1656 nt) opgD → glucans biosynthesis protein D
RA 1,517,587 2 bp→TT intergenic (+25/‑60) ortT → / → ydcY orphan toxin OrtT/DUF2526 domain‑containing protein YdcY
RA 1,522,583 C→T Q161Q (CAG→CAA yncD ← putative TonB‑dependent outer membrane receptor
RA 1,541,037 A→G I518I (ATT→ATC narZ ← nitrate reductase Z subunit alpha
RA 1,541,058 G→A T511T (ACC→ACT narZ ← nitrate reductase Z subunit alpha
RA 1,591,711 C→A E156* (GAG→TAG)  hipA ← serine/threonine‑protein kinase toxin HipA
RA 1,629,247 C→T I11I (ATC→ATT ydfZ → putative selenoprotein YdfZ
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,648,428 T→C intergenic (+87/‑80) dicA → / → ydfA DNA‑binding transcriptional dual regulator DicA/DUF1391 domain‑containing protein YdfA
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,660,102 C→T N678N (AAC→AAT ynfE → putative selenate reductase YnfE
RA 1,664,866 C→T Q121* (CAA→TAA)  dmsD → redox enzyme maturation protein DmsD
RA 1,667,460 G→A Q369* (CAG→TAG)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,685,542 (C)6→7 coding (1047/1404 nt) fumC ← fumarase C
RA 1,712,661 A→T intergenic (+503/‑108) nth → / → dtpA endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
RA 1,714,326 (T)9→8 intergenic (+55/‑51) dtpA → / → gstA dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA
RA 1,748,841 C→T R240K (AGA→AAA)  ydhT ← uncharacterized protein YdhT
RA 1,808,014 G→A V73V (GTG→GTA yniA → putative kinase YniA
RA 1,836,397 G→A E109K (GAG→AAG)  ynjB → putative ABC transporter periplasmic binding protein YnjB
RA 1,848,300 2 bp→TT coding (376‑377/552 nt) ydjA ← putative oxidoreductase YdjA
RA 1,878,937 G→A G22R (GGA→AGA)  yoaJ → uncharacterized protein YoaJ
RA 1,894,675 C→T R19R (CGG→CGA yoaH ← UFP0181 family protein YoaH
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 2,023,559 A→G T223A (ACT→GCT)  fliR → flagellar biosynthesis protein FliR
RA 2,027,332 T→A E229V (GAA→GTA)  dgcQ ← diguanylate cyclase DgcQ
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,041,278 G→A intergenic (+160/‑97) msrQ → / → zinT membrane‑bound flavocytochrome MsrQ/metal‑binding protein ZinT
RA 2,052,885 G→A intergenic (+871/‑758) yeeJ → / → shiA inverse autotransporter adhesin/shikimate:H(+) symporter
RA 2,090,780 C→T Q197* (CAA→TAA)  hisG → ATP phosphoribosyltransferase
RA 2,104,024 G→T I162I (ATC→ATA wbbK ← putative glycosyltransferase WbbK
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,120,969 G→A L196L (CTG→TTG)  wcaJ ← UDP‑glucose:undecaprenyl‑phosphate glucose‑1‑phosphate transferase
RA 2,139,387 (G)5→6 coding (1486/1584 nt) yegH → inner membrane protein YegH
RA 2,142,598 G→A L117L (CTC→CTT udk ← uridine/cytidine kinase
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,173,448 C→T intergenic (‑575/+834) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,233,609 C→T Q4* (CAG→TAG)  yeiS → DUF2542 domain‑containing protein YeiS
JC JC 2,239,316 IS5 (–) +4 bp intergenic (‑27/+31) mglA ← / ← mglB D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit/D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein
RA 2,242,247 C→T G241R (GGG→AGG)  yeiB ← DUF418 domain‑containing protein YeiB
RA 2,247,134 A→C Y467D (TAC→GAC)  lysP ← lysine:H(+) symporter
RA 2,361,210 C→G E69Q (GAA→CAA)  rhmD ← L‑rhamnonate dehydratase
RA 2,404,402 G→A S71L (TCG→TTG)  nuoB ← NADH:quinone oxidoreductase subunit B
RA 2,420,990 (C)6→7 coding (370/648 nt) yfcG → disulfide bond oxidoreductase YfcG
RA 2,481,608 A→G L190S (TTA→TCA)  emrY ← tripartite efflux pump membrane subunit EmrY
RA 2,498,392 A→C intergenic (‑97/‑279) alaC ← / → pyrS glutamate‑‑pyruvate aminotransferase AlaC/sensor histidine kinase PyrS
RA 2,546,804 T→A D44E (GAT→GAA murP → N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,577,937 T→C E147E (GAA→GAG maeB ← malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
RA 2,676,087 A→G S247P (TCT→CCT)  yphD ← putative ABC transporter membrane subunit YphD
RA 2,745,600 A→G Y107H (TAC→CAC)  rimM ← ribosome maturation factor RimM
RA 2,774,947 G→A G544S (GGT→AGT)  yfjW → uncharacterized protein YfjW
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,880,635 T→C L138L (TTA→TTG casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 3,025,601 (T)5→4 coding (1251/1401 nt) xanQ → xanthine:H(+) symporter XanQ
RA 3,070,858 T→G K129N (AAA→AAC fbaA ← fructose‑bisphosphate aldolase class II
MC JC 3,131,341 Δ128 bp IS5‑mediated yghQ ← putative transport protein YghQ
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,256,301 G→A S117S (TCC→TCT cyuA ← putative L‑cysteine desulfidase CyuA
RA 3,279,276 C→G F121L (TTC→TTG kbaZ → putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone
RA 3,296,481 G→A V25M (GTG→ATG)  dolP → division and outer membrane stress‑associated lipid‑binding lipoprotein
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,411,689 G→T E13* (GAA→TAA)  yhdJ → DNA adenine methyltransferase
RA 3,433,900 C→T L71L (CTA→TTA)  def → peptide deformylase
RA 3,441,425 C→A G84V (GGC→GTC)  rpsD ← 30S ribosomal subunit protein S4
RA 3,457,209 C→T A278V (GCC→GTC)  gspD → Type II secretion system protein GspD
RA 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,486,175 G→A C19Y (TGC→TAC)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,486,502 C→T T128I (ACT→ATT)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,507,249 C→T D30N (GAC→AAC)  yhfT ← uncharacterized protein YhfT
RA 3,511,466 C→T V293V (GTG→GTA yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,555,047 2 bp→TG coding (1963‑1964/2706 nt) malT → DNA‑binding transcriptional activator MalT
RA 3,560,362 G→A intergenic (+496/+260) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,646,970 G→T Q224H (CAG→CAT gor → glutathione reductase (NADPH)
RA 3,647,428 C→T A377V (GCG→GTG)  gor → glutathione reductase (NADPH)
RA 3,679,581 G→A G55R (GGA→AGA)  kdgK → 2‑dehydro‑3‑deoxygluconokinase
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,719,429 G→A intergenic (‑385/‑49) yiaF ← / → yiaG DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,729,440 2 bp→TT noncoding (105‑106/160 nt) xylZ ← small RNA XylZ
RA 3,730,560 C→T W69* (TGG→TAG)  xylA ← xylose isomerase
RA 3,770,539 C→T V281M (GTG→ATG)  yibH ← inner membrane protein YibH
RA 3,773,173 Δ1 bp coding (893/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,773,176 C→T S299F (TCT→TTT)  mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,817,167 C→T T164T (ACC→ACT yicC → putative RNase adaptor protein YicC
JC JC 3,890,715 IS1 (–) +9 bp coding (480‑488/1248 nt) tnaB → tryptophan:H(+) symporter TnaB
RA 3,926,991 G→A L5L (CTG→TTG)  asnC ← DNA‑binding transcriptional dual regulator AsnC
RA 3,931,779 T→C F155S (TTT→TCT)  kup → K(+):H(+) symporter Kup
RA 4,153,836 G→A Q338* (CAA→TAA)  argE ← acetylornithine deacetylase
MC JC 4,180,661 Δ6 bp coding (102‑107/366 nt) rplL → 50S ribosomal subunit protein L12
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,215,670 G→T D65Y (GAT→TAT)  aceB → malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,331,890 C→T L463L (CTC→CTT proP → osmolyte:H(+) symporter ProP
RA 4,437,890 C→T Q62* (CAG→TAG)  ytfI → protein YtfI
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,476,605 G→A S390N (AGC→AAC)  yjgL → protein YjgL
RA 4,489,016 C→T E350K (GAA→AAA)  yjgR ← DUF853 domain‑containing protein YjgR
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
JC JC 4,542,042 IS1 (+) +8 bp coding (6‑13/597 nt) fimE → regulator for fimA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 361657 362203 547 94 [1] [0] 90 [lacA]–[lacY] [lacA],[lacY]
* * ÷ NC_000913 1978484 1979270 787 110 [0] [0] 113 insB5–insA5 insB5,insA5

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 19796 =NA (NA)93 (1.020) 64/588 0.1 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 361656 0 (0.000)coding (205/612 nt) lacA galactoside O‑acetyltransferase
* ? NC_000913 = 258675NA (NA)90 (0.990) 61/590 0.2 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 362204 = 0 (0.000)coding (976/1254 nt) lacY lactose permease
* ? NC_000913 1207790 =42 (0.460)61 (0.710) 43/558 0.7 59.3% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 44 (0.510)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780540 (0.440)51 (0.590) 40/558 0.8 55.5% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 44 (0.510)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 19784830 (0.000)110 (1.200) 63/590 0.2 100% intergenic (‑286/+35) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
?NC_000913 = 2070271 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 1979271 =0 (0.000)107 (1.230)
+TTAAGTAATTGAGTG
66/560 0.1 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 2996360 = NA (NA)intergenic (+2504/+12) ygeI/insD4 protein YgeI/IS2 insertion element protein InsB
* ? NC_000913 4542682 =11 (0.120)138 (1.560) 75/572 0.0 93.0% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 10 (0.110)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269020 (0.220)128 (1.450) 73/572 0.0 89.7% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 10 (0.110)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit