Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,685,542	(C)6→7	coding (1047/1404 nt)	fumC ←	fumarase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,685,542	1	.	C	86.6%	211.1 / 26.8	67	coding (1047/1404 nt)	fumC	fumarase C
Reads supporting (aligned to +/- strand):  ref base . (4/5);  new base C (23/35);  total (27/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACT  >  NC_000913/1685248‑1685836                                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                       ttCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑c‑c‑c‑c‑cccatgt                                                                                                                                                                                                                                                                                                    <  1:363410/300‑6 (MQ=255)   cGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAGGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAGTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGtt                                                                                                                                                                                                                                                                                                   >  2:13029/1‑299 (MQ=255)   cGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATCGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGtt                                                                                                                                                                                                                                                                                                   >  2:13722/1‑299 (MQ=255)   cGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTATTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATATCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCACCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGCAGAAGTTCAGTTCAAAGTTACAGGAAGC‑GTC‑C‑C‑C‑CC‑ATGtt                                                                                                                                                                                                                                                                                                   >  2:12947/1‑299 (MQ=255)    gttacaaTACCCTACCGCAAGGGTAGCATCTATTAAGGGCAGCCCTGCTTTATGCTCTTCTTTTGCGATCTCGGCGGCTTTGTCATAATCAATGTGAGGGGTAAGCGCGGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTGCAATACCCACTGCGCAGTGTT‑GTGTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGTAAGC‑G‑C‑C‑C‑C‑CCCATGTTg                                                                                                                                                                                                                                                                                                  <  2:181955/293‑1 (MQ=255)        aGATACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGc                                                                                                                                                                                                                                                                                             >  1:366472/1‑300 (MQ=255)        aGATACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑AACC‑ATGTTGATCGc                                                                                                                                                                                                                                                                                             >  1:198008/1‑300 (MQ=255)            aCCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCAc                                                                                                                                                                                                                                                                                          >  1:560196/1‑299 (MQ=255)                  gcgcAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAANGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTc                                                                                                                                                                                                                                                                                   <  1:511122/300‑1 (MQ=255)                        gggCCTCAGCTTTTAAGTTCAGCCCTTCTTTGTGCGCTTTTTTGTCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑CTC‑C‑C‑CC‑ATGt                                                                                                                                                                                                                                                                                                    <  2:62232/277‑1 (MQ=255)                                gCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑CAC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAca                                                                                                                                                                                                                                                                     >  1:264904/1‑300 (MQ=255)                                  ttttAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagc                                                                                                                                                                                                                                                                   >  1:699430/1‑300 (MQ=255)                                    ttAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGTCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                                                                                                                                                                                                 <  1:359023/300‑1 (MQ=255)                                    ctgcgagatgtgtataagagacagcTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGANGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagca                                                                                                                                                                                                                                                                  >  1:509208/24‑299 (MQ=255)                                        gttcatctcgtcgttATGCGCTTTTTTGTCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGGTAAGCGCAGTCACCAGCATTAGCGATTCATTGAGGAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCATTGTTTGT‑TAAAACGTTCCATGCCATCTGCCAGCAAGCGCACCGAGTGAAGGAAAGGGTGGATAAACATGGGACGGAGGACGGTGAGGTCAGAGTTATCGGAAGG‑G‑C‑CTC‑C‑CC‑ATGTTg                                                                                                                                                                                                                                                                                                  <  2:181969/250‑1 (MQ=255)                                             gCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGtt                                                                                                                                                                                                                                                        >  1:216691/1‑300 (MQ=255)                                             gCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGAGTGCAGGGAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGTAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCaga                                                                                                                                                                                                                                                                >  2:696928/1‑292 (MQ=255)                                                    tttATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                                                                                                                                                                                                  <  1:166701/299‑1 (MQ=255)                                                         gcgcTTTTTTGTCGATCTCTGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCacac                                                                                                                                                                                                                                            <  2:695800/300‑1 (MQ=255)                                                         gcgcTTTTTTGGCGATCTCGTCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCacac                                                                                                                                                                                                                                            <  1:403737/300‑1 (MQ=255)                                                         gcgcTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCacac                                                                                                                                                                                                                                            <  1:434091/300‑1 (MQ=255)                                                                   ggCGATCTCGGCGGCGTTGTCATAACCAATGTGGGTGGTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGt                                                                                                                                                                                                                                  <  2:130700/300‑1 (MQ=255)                                                                      gATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGt                                                                                                                                                                                                                                  <  1:108434/297‑1 (MQ=255)                                                                      gATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGAGTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGt                                                                                                                                                                                                                                  <  1:434402/297‑1 (MQ=255)                                                                        tctcGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCTGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGt                                                                                                                                                                                                                                  <  2:263366/295‑1 (MQ=255)                                                                                gcTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTcccc                                                                                                                                                                                                                       >  1:393199/1‑298 (MQ=255)                                                                                           aCCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCGTTGAGTAATTGATTGATTCGTTCACGATTCGGTTCCATACCCACTGCGCAGTGTTTGT‑TAAAATTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGGAATTGTGGATCACCATTGGACGGAAGATGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCACATCACCTGACAGCAGAGCATGATTAATGCCTAACACTGTGTTGGGTTCAATTTCAACGGCATGATTGAGc                                                                                                                                                                                                          >  2:117333/1‑300 (MQ=255)                                                                                           aCCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑CAC‑CA‑ATGTTGATCGCCACGTCGTTCCCCATCAACTGACAGCAGAGCATGGTTAATGCCTAAAACTGTGTTGGGTTCACTTTCCACGGCATGATTGAGc                                                                                                                                                                                                          >  2:117128/1‑300 (MQ=255)                                                                                           aCCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGc                                                                                                                                                                                                          >  1:571655/1‑300 (MQ=255)                                                                                           aCCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGc                                                                                                                                                                                                          >  2:698250/1‑300 (MQ=255)                                                                                                     gtgtTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                                                                                                                                                                                                <  1:475052/300‑1 (MQ=255)                                                                                                     gtgtTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                                                                                                                                                                                                >  2:117680/1‑300 (MQ=255)                                                                                                       gtTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCAt                                                                                                                                                                                              >  2:686020/1‑300 (MQ=255)                                                                                                       gtTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTAACACTGTGTTGGGTTCACTTTCCACGGCATGATTGAGCTGCCCGGCTCAt                                                                                                                                                                                              >  2:686025/1‑300 (MQ=255)                                                                                                                 gTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGAt                                                                                                                                                                                    >  1:541648/1‑300 (MQ=255)                                                                                                                       aGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGAt                                                                                                                                                                              >  2:709411/1‑300 (MQ=255)                                                                                                                                                    gattCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAgg                                                                                                                                                 <  1:244983/300‑1 (MQ=255)                                                                                                                                                    gattCGCGCACGATTAGGGTCAAGACCCACTGCGCAGGGTTGGG‑TAAAGCTTTCCATGCCACCTGCCAGCAAGCGAACCGATGGCAGGAAATTGTGGATCACCATTGGGCGGAAGACGTTCAGTTCAAAGTGACCGGAAGCAC‑C‑C‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACGGCAGAGCATGGTTAATGCCTTACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCTGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCatatg                                                                                                                                                 <  2:241892/300‑5 (MQ=255)                                                                                                                                                    gatgcGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAgg                                                                                                                                                 <  1:696928/296‑1 (MQ=255)                                                                                                                                                           tCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGNAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGgccagcc                                                                                                                                           <  1:518071/299‑1 (MQ=255)                                                                                                                                                                 tttgtttaaccccccACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATc                                                                                                                                                                                                                                                                                               <  1:652276/133‑1 (MQ=255)                                                                                                                                                                    ggTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑CACC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGAcatc                                                                                                                                 <  1:49103/300‑1 (MQ=255)                                                                                                                                                                         aaTACCCACTGCGTAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGTAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCAGTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTgg                                                                                                                            <  2:366472/300‑1 (MQ=255)                                                                                                                                                                            aCCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGa                                                                                                                         >  1:51606/1‑300 (MQ=255)                                                                                                                                                                              ccACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGAtt                                                                                                                       >  2:415166/1‑300 (MQ=255)                                                                                                                                                                                     gaaGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTAGATCACCATTGGACGGAAGACGTTCAGTTCAAAGCTACCATAAGC‑G‑C‑C‑C‑C‑CCCATGTGGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTGCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACAAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACGTCATTGGCGATTTtcatc                                                                                                                 <  2:656262/297‑1 (MQ=255)                                                                                                                                                                                                   aaaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCaa                                                                                                   >  2:671803/1‑300 (MQ=255)                                                                                                                                                                                                                               gcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACTTCGTTCCCCACCACCTGACATCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCTGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAAcc                                                                       <  2:253982/300‑1 (MQ=255)                                                                                                                                                                                                                               gcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAAAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCTGCATGATTGAGCTGCCCGGCTCATTTTCCTGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAAcc                                                                       <  2:32646/300‑1 (MQ=255)                                                                                                                                                                                                                                    atttGCAGGAAATGGTGGGCCACCATTGGACGGAAGACGTTCGGTTCAAAGTTCCCGGAATTTC‑C‑C‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCTGTGCGCCTGGACCAGGGc                                                                  <  2:696953/298‑1 (MQ=255)                                                                                                                                                                                                                                          aGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATcac                                                             <  1:709411/299‑1 (MQ=255)                                                                                                                                                                                                                                          aGGAAATTGTGAATCACCATTGGACGTAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCATTCCCCATCACCTGACAGCAGAACATGTTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTTAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTGCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATcac                                                             <  2:403971/299‑1 (MQ=255)                                                                                                                                                                                                                                            gAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTNGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAgg                                                          <  1:141991/300‑1 (MQ=255)                                                                                                                                                                                                                                                   ttgATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCTGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAg                                                   <  1:456806/298‑1 (MQ=255)                                                                                                                                                                                                                                                   tgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACANCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAg                                                   <  2:572586/300‑1 (MQ=255)                                                                                                                                                                                                                                                   tgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAg                                                   <  1:456673/300‑1 (MQ=255)                                                                                                                                                                                                                                                    ggATCACCATGGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGACCGCCACGTCTTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGTTTCACTTTCCCCTGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGTCCAGATGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAgc                                                  <  2:198008/300‑1 (MQ=255)                                                                                                                                                                                                                                                     gATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAgc                                                  >  1:491149/1‑299 (MQ=255)                                                                                                                                                                                                                                                          ccATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCa                                            >  1:541264/1‑300 (MQ=255)                                                                                                                                                                                                                                                          ccATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCAAAGGTCGCCAGCGCtt                                              >  1:196737/1‑298 (MQ=255)                                                                                                                                                                                                                                                              tGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTTAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTTAAAtt                                        <  1:452959/300‑1 (MQ=255)                                                                                                                                                                                                                                                              tGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAAtt                                        <  1:452993/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 ccgagatgtgtataagagacagc‑c‑c‑cc‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACa                       >  1:651529/23‑300 (MQ=255)                                                                                                                                                                                                                                                                                   aaaGTTACCGTAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCATAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTTAGATTTCACCAATTCCGCAGCGNGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAAc                    <  2:509208/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aaaGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCTGCATGATTGAGCTGCCCTGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGNGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAAc                    <  2:516669/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aaaGTTACCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAAc                    <  1:49935/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                         aCCGGAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCANTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGTGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGcac             <  2:560196/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            ggAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATG‑TTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAgg          <  2:704154/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                            ggAAGC‑G‑C‑C‑C‑C‑CCCATGTTGATCGCCACGTCGCTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCTGCTCATTTTCCGGGATTGANATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTGCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAgg          <  2:216691/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       c‑c‑c‑cc‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACt  >  2:638201/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                       c‑c‑c‑cc‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCAAAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACt  >  2:638230/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                       TTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGT‑TAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACT  >  NC_000913/1685248‑1685836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑