Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,027,332	T→A	E229V (GAA→GTA)	dgcQ ←	diguanylate cyclase DgcQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,027,332	0	T	A	97.5%	240.3 / NA	79	E229V (GAA→GTA)	dgcQ	diguanylate cyclase DgcQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (43/34);  minor base C (2/0);  total (45/34)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGACTGTCCATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATC  >  NC_000913/2027037‑2027627                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        gCGACTGTCCATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCgg                                                                                                                                                                                                                                                                                                     <  1:48434/300‑1 (MQ=255)       gTCCATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGa                                                                                                                                                                                                                                                                                               >  2:711284/1‑300 (MQ=255)       gTCCATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGa                                                                                                                                                                                                                                                                                               >  1:42788/1‑300 (MQ=255)           aTACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTCTCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCANAGCACGCCGTACCAGTAGTTATTACTGTCTAACGGAACACTGACGGTCACCTGCGGTTCAGTATTGCTGGCGTGTCCCGGTTGCGaaat                                                                                                                                                                                                                                                                                            >  2:568107/1‑297 (MQ=255)                 aTGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCa                                                                                                                                                                                                                                                                                      >  2:371238/1‑299 (MQ=255)                 aTGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCAGTTGCGAAGTGAACCAg                                                                                                                                                                                                                                                                                     <  2:667068/300‑1 (MQ=255)                 aTGCCGCCCCGCGTATCATGTTCCCTTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGATCAGGATTGGAAAAGTTCAAAAATCTCAGCTTACTGTCATAGAGCTGATCCTCACCATCGGGGTTTTTATCGATGGCGTTTCTTACCAATTGCTGCATAGTACACACGGGAATACTCATCCCCAGCCCACCATACCAGTAGTTATTACTGTCTACAGGAATACTAACGGTACCCTGTGGTTTAGCATTGCTGGCGTGTACCGGTTGCGAAATGCACCa                                                                                                                                                                                                                                                                                      >  2:370712/1‑299 (MQ=255)                      gccCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGt                                                                                                                                                                                                                                                                                  <  1:430349/298‑1 (MQ=255)                                tCATGTTCCATTGCCTNCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTCTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGNACCGGTTGCGAAGTGAACNAGCGTACCGCGCGGTGa                                                                                                                                                                                                                                                                      >  1:610706/1‑300 (MQ=255)                                            gCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGATGAAGATTTtctagt                                                                                                                                                                                                                                                          >  2:200356/1‑297 (MQ=255)                                                     aGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTAATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCCGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAAACTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTAGTTGTGAAt                                                                                                                                                                                                                                                   >  2:396311/1‑298 (MQ=255)                                                               gTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCTGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAAcc                                                                                                                                                                                                                                       <  2:699535/300‑1 (MQ=255)                                                               gTTCACGAGGATCAAAAATATTCCATGTTGGATGGTCAGGATTGGAAGCGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCAGCGGTTCAGTATTGCTGGCGTGTCCCGGTTGCGCAGTGAACCAGTGTACCGCGCGGTGACGATTTTCTAGTTGTGAATGGCCaacaa                                                                                                                                                                                                                                          >  2:295732/1‑294 (MQ=255)                                                                     gAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTCTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGt                                                                                                                                                                                                                                  <  2:610706/299‑1 (MQ=255)                                                                         agcAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGt                                                                                                                                                                                                                             <  1:711284/298‑1 (MQ=255)                                                                            aaaaaTATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTTTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGAc                                                                                                                                                                                                                          <  2:337349/300‑1 (MQ=255)                                                                               aaTATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTAAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATaa                                                                                                                                                                                                                                          >  2:205386/1‑281 (MQ=255)                                                                                       cTGTTGGATGGTCAGGNTTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAANGGCCAATAAACCAGGGTTNGGTGACATAGCCGtaat                                                                                                                                                                                                               >  1:603138/1‑300 (MQ=255)                                                                                       cTGTTGGATGGTCAGGNTTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAANGGCAAATAAACCAGGGTTNGGTGACATAGCCGtaat                                                                                                                                                                                                               >  1:603410/1‑300 (MQ=255)                                                                                       cTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGtaat                                                                                                                                                                                                               >  1:192013/1‑300 (MQ=255)                                                                                         gTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGtaataa                                                                                                                                                                                                             <  1:345534/300‑1 (MQ=255)                                                                                                ggTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGtt                                                                                                                                                                                                       >  1:366708/1‑299 (MQ=255)                                                                                                      gATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTCATAACGCGTTGGAACAt                                                                                                                                                                                                >  2:684849/1‑300 (MQ=255)                                                                                                      gATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACAt                                                                                                                                                                                                >  1:233553/1‑300 (MQ=255)                                                                                                       aTTGGAATAGGTCAAAAACCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACTGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACAt                                                                                                                                                                                                <  2:468801/299‑1 (MQ=255)                                                                                                                     aaaaTCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAgg                                                                                                                                                                                 <  1:585808/300‑1 (MQ=255)                                                                                                                         tctcAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTc                                                                                                                                                                               >  1:380915/1‑298 (MQ=255)                                                                                                                           tcAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGc                                                                                                                                                                            <  1:411000/299‑1 (MQ=255)                                                                                                                                     gTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAAc                                                                                                                                                                                     >  2:395476/1‑280 (MQ=255)                                                                                                                                     gTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAAc                                                                                                                                                                                     <  1:395476/280‑1 (MQ=255)                                                                                                                                     gTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAAc                                                                                                                                                                 >  1:684080/1‑300 (MQ=255)                                                                                                                                          agagCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACactgtctcttatacacatctcgcag                                                                                                                                                            <  1:643216/300‑25 (MQ=255)                                                                                                                                            agCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGTGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTAGGCTGCGTCGAAACGTAAAAt                                                                                                                                                          >  2:64123/1‑300 (MQ=255)                                                                                                                                            agCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAAt                                                                                                                                                          >  1:308605/1‑300 (MQ=255)                                                                                                                                                gATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGACCCAGTGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAAACAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACATAAAATcc                                                                                                                                                        >  2:352567/1‑298 (MQ=255)                                                                                                                                                              aGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGNAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGacatac                                                                                                                                        <  1:509797/300‑1 (MQ=255)                                                                                                                                                               ggTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGacatac                                                                                                                                        <  1:670707/299‑1 (MQ=255)                                                                                                                                                               ggTTTTGATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCTGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGacataca                                                                                                                                       <  2:360365/300‑1 (MQ=255)                                                                                                                                                                    ttATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGcc                                                                                                                                  <  2:175821/300‑1 (MQ=255)                                                                                                                                                                            ggCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAAc                                                                                                                          >  1:554059/1‑300 (MQ=255)                                                                                                                                                                               gTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTANCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGAAATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCa                                                                                                                        <  1:518319/299‑1 (MQ=255)                                                                                                                                                                                         aaTTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACgaggag                                                                                                             <  1:224027/300‑1 (MQ=255)                                                                                                                                                                                          aTTGCTGCATAGTACGCACGGTAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTTTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACTAGGAGt                                                                                                            <  2:83675/300‑1 (MQ=255)                                                                                                                                                                                          aTTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCGGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGTGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCTAGACATACATCGCCTGTTCAACCATCGACGAGGAGt                                                                                                            <  2:83246/300‑1 (MQ=255)                                                                                                                                                                                           ttGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGtt                                                                                                           >  1:723689/1‑300 (MQ=255)                                                                                                                                                                                           ttGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGACTGTTCAACCATCGACGAGGAGtt                                                                                                           >  1:723771/1‑300 (MQ=255)                                                                                                                                                                                            ttctgcATAGTATGTATGGGAGTACGCATCTCTAGCACGTCAGACCGGTATTTATTGCTGTCTACCGGAACAATGACGGTAACCTGCGGTTCAGTATTGTTTGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGGGACAATTTTATCGTGGTGAAGGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAATGCGTTGGAACATTGCGCGTAAATAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCTAGACATACAGCGCTTGTTCAACCATCGACGAGGAGTtg                                                                                                          <  2:587588/298‑1 (MQ=255)                                                                                                                                                                                            tgctgcATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTtg                                                                                                          <  2:587667/300‑1 (MQ=255)                                                                                                                                                                                                gcATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTgc                                                                                                       >  1:721908/1‑299 (MQ=255)                                                                                                                                                                                                  aTAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGNGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACTAGGAGTTGTgcgc                                                                                                     <  2:218830/299‑1 (MQ=255)                                                                                                                                                                                                  aTAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTgcgc                                                                                                     <  1:8168/299‑1 (MQ=255)                                                                                                                                                                                                            cgagAATACCCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACACTGCGCGTCAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACCTCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCgcagca                                                                                          >  2:16008/4‑300 (MQ=255)                                                                                                                                                                                                            cGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCACCCCTCGACGAGGAGTTGTGCGCTAGTCgcagca                                                                                          >  2:15532/1‑300 (MQ=255)                                                                                                                                                                                                              ggAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCCGTATTGCTGGCGTGTACCGGTTGAGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTAGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGAAGAGGAGTTGTGCGCTCGTCGCAGCaa                                                                                         >  2:8051/1‑299 (MQ=255)                                                                                                                                                                                                                       aTCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGTTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGTTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGTTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAAc                                                                                <  1:112578/299‑1 (MQ=255)                                                                                                                                                                                                                       aTCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAAc                                                                                <  1:111919/299‑1 (MQ=255)                                                                                                                                                                                                                                aCGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGc                                                                       <  1:438989/299‑1 (MQ=255)                                                                                                                                                                                                                                 cGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGGCGATTTTCTCGTTGTGAATGGCCAATAAAACAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCa                                                                      >  2:678812/1‑299 (MQ=255)                                                                                                                                                                                                                                 cGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTACAGTGCa                                                                      >  2:587007/1‑299 (MQ=255)                                                                                                                                                                                                                                          agtagtTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCGGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGTTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAAtt                                                             <  2:431440/299‑1 (MQ=255)                                                                                                                                                                                                                                            tagtTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCa                                                          <  1:469234/300‑1 (MQ=255)                                                                                                                                                                                                                                               ttattaCTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCAtt                                                        >  2:589181/1‑299 (MQ=255)                                                                                                                                                                                                                                               ttattaCTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCAtt                                                        >  1:714895/1‑299 (MQ=255)                                                                                                                                                                                                                                                                            aaCCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGgag                          <  1:106070/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            aaCCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGAGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGgag                          <  1:200356/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    gTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCt                  >  1:109643/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    gTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGATGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTcccc                   >  2:413770/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                         gTATTGCTGGTGTGTACCGGTTGCGAAGTGAACCAGCATACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATCAACCAAGGTTGGGTGACATAGCCATAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCCGCGTCGAAACGTAAAATCCGGCACNCGCGACATACACCGCCTGTTCAACCATCGACGGGGAGTTGTGCGCTAGTCGCCGAAAGTAACCAACTTCCAGTGCAGCGGTAATTTTATTGTCGAGGGTTTCAGTTTCGCGAGAAAGGAGATTCCCCTCGc               >  2:223325/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                         gTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTcccatagct              >  2:294950/1‑293 (MQ=255)                                                                                                                                                                                                                                                                                         gTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCt              >  1:701204/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                         gTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTAGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACNCGAGACATACATCGCCTGTTCAAACATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGACTTTCCTTTTCGCGCTACAGGAGATTCACCTCGCt              >  2:230773/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                            ttGCTGGCGTGTACCGGTTGCTAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACt          <  1:396311/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            ttGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAACATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCNAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATCTTCGCGAGACAGGAGATTCCCATCGCTGACt          >  2:564347/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 ggCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGc     <  1:64123/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  gCGTGTACCGGTTGCGAAGTGAACCAGCATACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAAAGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGACACGCAAAACCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTTCGCTAGTCGCAGCAAGTAACCAACTTCCCATGCCGCGGTAATTTCATTGTCGAGGc                                                >  2:439061/1‑256 (MQ=255)                                                                                                                                                                                                                                                                                                   cGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCCCGCGAGACATACATCGACTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCAt   >  2:279343/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   cGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGAAGAGGAGTTGTGCGCTAGTCGCAGCAAGTCACCAACTTCCAGTGCAGCGGTAATTTCATTGCCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCACTCGCTGACTAATGCAt   >  2:279482/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   cGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACAAGGGTTGGGTGAAATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGCGACAGGAGATTCCCCTCGCTGACTAATGc     >  2:279613/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                    gtgtACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACCGGAGATTCCCCTCGCTGACTAATGCATc  >  2:727193/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        GCGACTGTCCATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATC  >  NC_000913/2027037‑2027627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑